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Offlinepj541
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Agar and growing that white stuff!
    #424216 - 10/13/01 04:40 PM (15 years, 1 month ago)

Hello, I am growing cultures from multi spore inoc and have a few questions.

1Mycelium is growing slow and light it also seems to be growing below the serface. I used 12.5g of MEA to 500ml h2o.
Is this the proper ratio of agar to water?

2. I transfered the rizomorphic sections and they have covered about 1/2 the plate but the growth is barly visible, it looks like the serface of the agar has just been textured.

What causes this?

thanks a lot PJ


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?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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Offlinesylo
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Re: Agar and growing that white stuff! [Re: pj541]
    #424731 - 10/14/01 02:24 AM (15 years, 1 month ago)

Most preparations call for at least 25g of MEA for 500 ml of H2O so you only have half enough. That may be your problem.


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Offlinepj541
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Re: Agar and growing that white stuff! [Re: sylo]
    #426307 - 10/15/01 02:36 PM (15 years, 1 month ago)

thanks, That makes sense but the vendor i bought from told me to use 12.5g for 500ml. He was having quite the stressful day.

Will the fact that the mix is low cause any problems other than slowed colinization time, or will it cause the formation of a weak strain?

I really need to know because this was to be my first shot at agar/grain/wormpoo/straw.

thanks PJ


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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Offlinepj541
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Re: Agar and growing that white stuff! [Re: pj541]
    #426316 - 10/15/01 02:44 PM (15 years, 1 month ago)

I still have 12.5g MEA do you guys think that it would be advisable to cook it up in the proper proportions and transfer again?

To see if i can get some heavier growth.

thanks PJ


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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Invisibleoscill8
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Registered: 02/27/01
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Re: Agar and growing that white stuff! [Re: sylo]
    #426370 - 10/15/01 03:31 PM (15 years, 1 month ago)

mea if you make it yourself is 20 g malt and 20 g agar per 1 L (TMC, p 20). so 40g mea to 1 L, 20 g mea to 500 mL. myc. should just grow on the surface... perhaps the ratio is your problem. likely your agar media is too thin and doesnt have the correct amount of nutrition (has about 60% of whats standard)... thats why its slower and thinner than it should be. also, you just may be working with a print thats difficult to germinate- but prolly its your agar mix more than anything :)

if you wanted to make media with your remaining 12.5 g, ... you could use 10 g of your mix to 250 mL of water to match the TMC's recipe of mea. id repour some new plates, do some transfers, and it should work. id also ask the vendor who told you the wrong recipe if they could replace the messed up batch for you as well.


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Offlinepj541
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Re: Agar and growing that white stuff! [Re: oscill8]
    #426486 - 10/15/01 06:06 PM (15 years, 1 month ago)

Thanx a lot Oscill I apreaciate it.


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?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



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