Home | Community | Message Board


World Seed Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Malt Extract, Paul Stamets

Jump to first unread post. Pages: 1
Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 16 years, 1 month
Agar and growing that white stuff!
    #424216 - 10/13/01 04:40 PM (16 years, 8 months ago)

Hello, I am growing cultures from multi spore inoc and have a few questions.

1Mycelium is growing slow and light it also seems to be growing below the serface. I used 12.5g of MEA to 500ml h2o.
Is this the proper ratio of agar to water?

2. I transfered the rizomorphic sections and they have covered about 1/2 the plate but the growth is barly visible, it looks like the serface of the agar has just been textured.

What causes this?

thanks a lot PJ


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 16 years, 1 month
Re: Agar and growing that white stuff! [Re: pj541]
    #424731 - 10/14/01 02:24 AM (16 years, 8 months ago)

Most preparations call for at least 25g of MEA for 500 ml of H2O so you only have half enough. That may be your problem.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 16 years, 1 month
Re: Agar and growing that white stuff! [Re: sylo]
    #426307 - 10/15/01 02:36 PM (16 years, 8 months ago)

thanks, That makes sense but the vendor i bought from told me to use 12.5g for 500ml. He was having quite the stressful day.

Will the fact that the mix is low cause any problems other than slowed colinization time, or will it cause the formation of a weak strain?

I really need to know because this was to be my first shot at agar/grain/wormpoo/straw.

thanks PJ


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 16 years, 1 month
Re: Agar and growing that white stuff! [Re: pj541]
    #426316 - 10/15/01 02:44 PM (16 years, 8 months ago)

I still have 12.5g MEA do you guys think that it would be advisable to cook it up in the proper proportions and transfer again?

To see if i can get some heavier growth.

thanks PJ


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleoscill8
*bondage fairy*
Registered: 02/27/01
Posts: 1,215
Re: Agar and growing that white stuff! [Re: sylo]
    #426370 - 10/15/01 03:31 PM (16 years, 8 months ago)

mea if you make it yourself is 20 g malt and 20 g agar per 1 L (TMC, p 20). so 40g mea to 1 L, 20 g mea to 500 mL. myc. should just grow on the surface... perhaps the ratio is your problem. likely your agar media is too thin and doesnt have the correct amount of nutrition (has about 60% of whats standard)... thats why its slower and thinner than it should be. also, you just may be working with a print thats difficult to germinate- but prolly its your agar mix more than anything :)

if you wanted to make media with your remaining 12.5 g, ... you could use 10 g of your mix to 250 mL of water to match the TMC's recipe of mea. id repour some new plates, do some transfers, and it should work. id also ask the vendor who told you the wrong recipe if they could replace the messed up batch for you as well.


--------------------
? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepj541
member
Registered: 02/04/01
Posts: 128
Loc: fuck off
Last seen: 16 years, 1 month
Re: Agar and growing that white stuff! [Re: oscill8]
    #426486 - 10/15/01 06:06 PM (16 years, 8 months ago)

Thanx a lot Oscill I apreaciate it.


--------------------
?Well, this is how the world works. All energies flow according to the whims of the great magnet. What a fool I was to defy him.?



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Malt Extract, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* using agar? downforpot 1,232 6 12/02/01 09:00 PM
by Anno
* When using agar dishes wouldn't it be wise too TheGreenLantern 1,005 3 04/13/02 03:35 PM
by ParticleMan
* the problems w/ friends.... Bob_J 994 11 04/25/02 04:04 PM
by Alien
* Agar questions-I may have goofed blahblahblah 1,409 6 01/11/02 04:58 PM
by ar393
* Re: Agar w/ h202, any info?? 3Mshroom 1,283 8 05/08/00 06:25 AM
by mycofile
* Agar + Oven Tek? Maddoc 956 5 07/22/01 06:37 AM
by Anonymous
* Growth problems. Blunderbuss 467 1 02/09/02 03:20 AM
by Glacius
* Shroom Growing Outside? TheJoker 2,258 17 06/17/01 01:50 AM
by Gilgamesh

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,043 topic views. 38 members, 192 guests and 29 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
High Mountain Compost
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.041 seconds spending 0.009 seconds on 19 queries.