I'm gonna give that one a try

The Lizard,

Link

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In the beginning the Universe was created...
This made a lot of people angry and was widely regarded as a bad move.
-- Douglas Adams, The Restaurant at the End of the Universe

So far it has worked perfectly for me. Fabulous colonization rates, MASSIVE pinhead formation.

1. Create a past of brown rice flour and water (or your choice of
substrate), spread evenly in the bottom of a canning jar, seal,
pressure cook at 15 psi for 30 minutes.
2. Pour about 1/3 cup of 3% h2o2 into a jar.
3. Obtain a clean coffee grinder; fill with water to the brim of
the metal cup, insert a fresh and cleaned mushroom stem
(no cap) and proceed to grind for 10-15 seconds.
4. Use a clean spoon to transfer some of the resulting mixture into
the h2o2 jar.
5. Let it fizzle for a couple minutes, then draw into a syringe.
6. Inoculate jar created in step#1, or simply inoculate pf-style
jars.

Tips if Culture Contaminates

1. Extract a piece of healthy mycelium.
2. Cut mycelium into tiny pieces with razor blade.
3. Transfer pieces to a small pool of h2o2 and then suck into
a syringe.
4. Inoculate another culture jar with it.

I'm guessing by the way, that organic rye flour/quinoa/etc. could be used as a substitute, I'll try and post my results.

-peace

BTW, for those of us that are anal, what is the amount of BRF/water to use for one 1/2 pt. jar?

-peace[/B][/QUOTE]

I think this one deserves the growing archives....

EXCELLENT!!!!!!!!!!

Another idea would be to use this to immedatly transfer to a liquid media like honey water or malt solution... again.. WOW!!!

ThE JafF

[This message has been edited by ThE JafF (edited February 15, 2000).]

Link

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In the beginning the Universe was created...
This made a lot of people angry and was widely regarded as a bad move.
-- Douglas Adams, The Restaurant at the End of the Universe

peace
camel

I would have skipped that step, but on my Western Family bottle of h2o2 it says that it should not be shaken. Doesn't give a reason, but I just assumed they know what they're talking about.

A whole lot of it, maybe most of it will be destroyed as the mushroom comes in contact with it. The way you do it, there is a lot larger peroxide/shroom ratio... So your way is probably a better way to do it after all..

I will say that I just did my first experiments with honey water.. Whoever came up with this deserves an applause... I got growth in just under 24 hours in 4 out of 6 jars (Rye flour/Vermiculite).. I am sure the other 2 are not far behind.

From 1 squirt (1 cc) of spore water I am sure one could innoculate 100 jars just by waiting 10 days for the honeywater to grow (from spores)..

I also reinoculated some more honey jars, and of course I have growth there as well.. Damn this is quick easy and cheap.. The real test is to wait a week and make sure nothing contaminates, but I have faith..

If it works well, I think that the combination of your technique and the honey will greatly improve yeild. Isolated strains perform sOOOOooooo much better.

So guess who's tek I will be trying next?

ThE JafF

[This message has been edited by ThE JafF (edited February 16, 2000).]

1/2 teaspoon per 250ml water... I just cook it up in 1/2 pints with the lids the right way, not inverted, and a hole for the syringe. I cover with foil while cooking, remove to inoculate, and immediatly cover with electrical tape. Works fantastic so far. I will keep everyone updated.. The important thing seems to be not to overcook the honeywater, or you will carmalize it.. So try pressure cooking for 20 min, that seems to work well.

ThE JafF

Also I have no idea what I was talking about before. You could still use peroxide in the coffee grinder and then transfer a spoon or two of this to more peroxide.. No reason to introduce any more contamination than necessary.

ThE JafF

ThE JafF

I got my eye out for a good method to go from spore print to liquid mycellium (honey or malt). This supposedly could be used to innoculate honey/malt mixture from mushroom tissue?

What would you do different if you had a spore syringe, just scrape some spores into the h202 jar?

thanks.

There are 3 major things you need to know about H2O2:

1. It gets broken down by mushroom/mycelial tissue when the cell walls are breached
2. Most growth medium also breaks it down unless heat treated.
3. It kills spores!

So if you put it in contact with spore prints or spore water then you will just kill the spores.

IMHO the recent enthusiasm about it's use in shroom growing is mostly from people who have not actually tried to use it. It has severe limitations.

I have developed a method which uses it at every stage, including in the actual growth medium. This virtually eliminates all contamination and means that you can colonise your substrate in the same trays that you fruit in. You have to use pressure cooked grain (wheat or rye) to prevent the H202 being degraded or you can use Weetabix (spun, whole grain pillows) as this is unreactive.

Having got it to work I have returned to using straight-forward agar in a ?5.00 home-made glovebox. It works out far simpler and quicker.

Rush Wayne's book on the subject has some methods which look promising for wood loving shrooms. It uses compressed-paper fuel pellets. He also have a recipe for H2O2 agar which is excellent for cloning.

Rad

that would be cluB99.

peace
hip

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[This message has been edited by Hippie3 (edited April 11, 2000).]

What do you do with the rice/water mixture after the mycelum grows?

Thanks.

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Away!

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Away!

Look, agar is just plain EASY, ok? PF tek only made it harder to teach people how to actually culture sterile mycelium properly.

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Visit My website!
http://titan.spaceports.com/~spore/

First agar..

Choose your agar mixture (there are hundreds of recipes to choose from) and prepare it. You can get premade mixtures, or mix your own.
note: this involves boiling all your ingrediants, sometimes straining out solids (depending on the recipe). You will need containers to do the agar work in, typically petri dishes, but wide mouth jars will work.. If you use plastic petri dishes, you must sterilize your agar mixture in a sperate container and somehow manage to pour it into your dishes, WHILE HOT! You must do this in a sterile location and keep them in a sterile location so they don't suck in dirty air while they cool.

Then once they solidify you just innoculate them, either by isolating a strain from another agar dish, cutting it out (hoping you have selected a single strain) and transfering it (in open air again) into the new fresh petri dish.

Of you can skip right to the culturing of a fresh mushroom to isolate a strain. This involes slicing open a stem and cutting out a section of the inside mycellium, and transfering to the fresh dish (all while praying your scalpel is clean and that nothing gets in your dish while it is open, or lands this clean piece of mycellium while you transfer it, or that you didn't contaminae it by touching the outside of the mushroom with ANYTHING once you sliced open the mushroom)...

And now you still have to wait until the dish grows out until you can use it. If there is contamination you have to keep trying to reisolate your strain from this dish into another dish. Then if you have managed to do this without contaminating it, you still have to take a risk again when you use this stuff to innoculate your substrate... either by usuing wedges (and openeing your substrate jars wide open) or blending it up.. (another open container)...
time... minimum 10 days... practically... 14-21 days.

Ok and now the new peroxide method..

Put piece of stem in coffee grinder with water or h2o2... No sterile conditions necessary...

Blend....No sterile conditions necessary...

Spoon into 1/3 cup peroxide...no steil conditions necessary...

Suck up with syring... No sterile conditions necessary...

Innoculate... time 5 minutes...

Yeah thats the same...

As a side note:

I do have quart jars of birdseed with all three of those species growing,(shitake, crimini, chantarelles) although I did make one stupid mistake... I bothered with the Chantarelles... the mycellium is growing, but I am pretty sure they can't be cultivated... I didn't realize this when I started.. Oh well... I will try anyway for the fun of it..

ThE JafF