I'm gonna give that one a try

The Lizard,

Link

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In the beginning the Universe was created...
This made a lot of people angry and was widely regarded as a bad move.
-- Douglas Adams, The Restaurant at the End of the Universe

So far it has worked perfectly for me. Fabulous colonization rates, MASSIVE pinhead formation.

1. Create a past of brown rice flour and water (or your choice of
substrate), spread evenly in the bottom of a canning jar, seal,
pressure cook at 15 psi for 30 minutes.
2. Pour about 1/3 cup of 3% h2o2 into a jar.
3. Obtain a clean coffee grinder; fill with water to the brim of
the metal cup, insert a fresh and cleaned mushroom stem
(no cap) and proceed to grind for 10-15 seconds.
4. Use a clean spoon to transfer some of the resulting mixture into
the h2o2 jar.
5. Let it fizzle for a couple minutes, then draw into a syringe.
6. Inoculate jar created in step#1, or simply inoculate pf-style
jars.

Tips if Culture Contaminates

1. Extract a piece of healthy mycelium.
2. Cut mycelium into tiny pieces with razor blade.
3. Transfer pieces to a small pool of h2o2 and then suck into
a syringe.
4. Inoculate another culture jar with it.

I'm guessing by the way, that organic rye flour/quinoa/etc. could be used as a substitute, I'll try and post my results.

-peace

BTW, for those of us that are anal, what is the amount of BRF/water to use for one 1/2 pt. jar?

-peace[/B][/QUOTE]

I think this one deserves the growing archives....

EXCELLENT!!!!!!!!!!

Another idea would be to use this to immedatly transfer to a liquid media like honey water or malt solution... again.. WOW!!!

ThE JafF

[This message has been edited by ThE JafF (edited February 15, 2000).]

Link

------------------
In the beginning the Universe was created...
This made a lot of people angry and was widely regarded as a bad move.
-- Douglas Adams, The Restaurant at the End of the Universe

peace
camel

I would have skipped that step, but on my Western Family bottle of h2o2 it says that it should not be shaken. Doesn't give a reason, but I just assumed they know what they're talking about.

A whole lot of it, maybe most of it will be destroyed as the mushroom comes in contact with it. The way you do it, there is a lot larger peroxide/shroom ratio... So your way is probably a better way to do it after all..

I will say that I just did my first experiments with honey water.. Whoever came up with this deserves an applause... I got growth in just under 24 hours in 4 out of 6 jars (Rye flour/Vermiculite).. I am sure the other 2 are not far behind.

From 1 squirt (1 cc) of spore water I am sure one could innoculate 100 jars just by waiting 10 days for the honeywater to grow (from spores)..

I also reinoculated some more honey jars, and of course I have growth there as well.. Damn this is quick easy and cheap.. The real test is to wait a week and make sure nothing contaminates, but I have faith..

If it works well, I think that the combination of your technique and the honey will greatly improve yeild. Isolated strains perform sOOOOooooo much better.

So guess who's tek I will be trying next?

ThE JafF

[This message has been edited by ThE JafF (edited February 16, 2000).]

1/2 teaspoon per 250ml water... I just cook it up in 1/2 pints with the lids the right way, not inverted, and a hole for the syringe. I cover with foil while cooking, remove to inoculate, and immediatly cover with electrical tape. Works fantastic so far. I will keep everyone updated.. The important thing seems to be not to overcook the honeywater, or you will carmalize it.. So try pressure cooking for 20 min, that seems to work well.

ThE JafF

Also I have no idea what I was talking about before. You could still use peroxide in the coffee grinder and then transfer a spoon or two of this to more peroxide.. No reason to introduce any more contamination than necessary.

ThE JafF

ThE JafF