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Invisiblenewuser1492
Registered: 06/12/03
Posts: 3,104
Flow hood question
    #3807779 - 02/20/05 07:25 PM (16 years, 8 months ago)

I've been looking at flow hood designs as I figure I'll need one eventually. Especially given my new found goal to grow vibrio phosphoreum.

All the pictures of flow hoods I've seen seem to have an open front with a hepa filter on the back and a blower on top. The mechanics seem nothing like the flow hoods from my chemistry class. The flow hoods there sucked air up and away from you through the partially open hood. How do the mycology flow hoods work if the front is completely open?

I thought a sterile environment would be more like a glove box with a hepa filter for air inlet. All outgoing air would be pulled through a blower. A single sealing door would be used to remove and return items. Once the box was sealed all air would flow through the hepa filter and out the blower giving a sterile environment.

I don't understand how the flow hoods I've seen are effective (Anno's as an example).


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OfflineAnnoA
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Re: Flow hood question [Re: newuser1492]
    #3808042 - 02/20/05 08:32 PM (16 years, 8 months ago)

They output a laminar flow of sterile air and you work in this sterile air.


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Invisiblenewuser1492
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Re: Flow hood question [Re: Anno]
    #3808082 - 02/20/05 08:42 PM (16 years, 8 months ago)

So the air is pushing towards you? If the blower is on top and the filter on the back how is the blower not pushing abient air directly at you?


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OfflineKaptKid
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Re: Flow hood question [Re: newuser1492]
    #3808084 - 02/20/05 08:42 PM (16 years, 8 months ago)

"The mechanics seem nothing like the flow hoods from my chemistry class. The flow hoods there sucked air up and away from you through the partially open hood."

Never seen one but sounds like you would be pulling contam's past the work.

How do the mycology flow hoods work if the front is completely open?


Flow of filtered air keeps comtam'sfrom falling into work.


--------------------
Child of the 60's, Tripping ever since.


:sun:


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OfflineAnnoA
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Re: Flow hood question [Re: newuser1492]
    #3808109 - 02/20/05 08:49 PM (16 years, 8 months ago)

> So the air is pushing towards you?
Yes.


> If the blower is on top and the filter on the back how is the blower not pushing abient air
>directly at you?

I don't know what you mean.



The blower blows the air through the HEPA filter.


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Invisiblenewuser1492
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Re: Flow hood question [Re: KaptKid]
    #3808120 - 02/20/05 08:53 PM (16 years, 8 months ago)

Never seen one but sounds like you would be pulling contam's past the work.

Biological contaminants weren't a problem. It was the evolution of toxic gas which was a problem. The hood sucked that gas up and away from you.

Hmm I think I'm thinking about the glove box backwards maybe?



I think I get it. The material you're working with goes in front of the hepa filter pictured above? Ahh I was thinking your workspace was actually enclosed like in a glove box.

Then what would the product I described in my post above be called? A pressure box?


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OfflineAnnoA
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Re: Flow hood question [Re: newuser1492]
    #3808149 - 02/20/05 09:01 PM (16 years, 8 months ago)

>The material you're working with goes in front of the hepa filter pictured above?

Exactly.


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InvisibleNewf
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Re: Flow hood question [Re: Anno]
    #3809066 - 02/21/05 01:12 AM (16 years, 8 months ago)

The hood you used in Chem class was a fume hood. Designed as you said to draw away potentially harmfull gases and fumes. Laminar flow hoods are what we use and they blow rather than suck.
Messing with chem= Fume hood
Messing with biology= Flow hood.


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