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Amazon Shop for: ½ Pint Jars, Agar, Salvia

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OfflineAnand
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Yello stuff on plant Tissue culture (Agar)
    #1408535 - 03/25/03 12:05 PM (13 years, 8 months ago)

i made some jars with agar and put plant pieces in it... some days later i got green contam.... but also some yellow stuff and i was wondering what the yellow stuff is






this last one is with pieces of salvia made on the same day/batch


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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1409200 - 03/25/03 04:41 PM (13 years, 8 months ago)

Cool looking.


--------------------
call me old-fashioned, but I think trains are totally kick ass


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Offlineneuro
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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1409497 - 03/25/03 06:45 PM (13 years, 8 months ago)

If you're going to make plant tissue cultures scrape tissue from the inside of the stem and smear it on the agar, more prone to contams if you just stick blocks of stem.


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Re: Yello stuff on plant Tissue culture (Agar) [Re: neuro]
    #1409835 - 03/25/03 09:05 PM (13 years, 8 months ago)

Als antwoord op:

If you're going to make plant tissue cultures scrape tissue from the inside of the stem and smear it on the agar, more prone to contams if you just stick blocks of stem.




thanks for the advice i'll try it the next time.

the pieces were put in household-bleach water solution and then rinsed with sterile water.
the next time i'll try also by adding some H2O2 to the Agar


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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1412007 - 03/26/03 05:20 PM (13 years, 8 months ago)

I forgot to say before did you add any plant hormones to the agar? You're not gonna get anything if you don't add any hormones.

I'm assuming you know something about what i'm talking about:

[IAA (rootting hormone)] > [CK (Cytokinin)] you'l get roots and no shoots from your culture

[CK] > [IAA] you'll get shoots and no roots from your culture

[CK] = [IAA] you'll get what is called a callus, it's a mass of fluffy white cells called parenchyma cells. Parenchyma cells are generic non descript plant cells.



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OfflineAnnoA
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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1412646 - 03/26/03 09:46 PM (13 years, 8 months ago)



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OfflineAnand
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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anno]
    #1412828 - 03/26/03 11:42 PM (13 years, 8 months ago)

i know that that book would be very usefull but i just can't get a creditcard here in holland (you need a above average income) so ordering at Amazon.com is not realy an option. A bookstore in Amsterdam sells the book but costs twice as much as on Amazon.

for the Agar i made i used a guide that was on the net: http://www.une.edu.au/~agronomy/AgSSrHortTCinfo.html


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Offlineneuro
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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1413901 - 03/27/03 10:37 AM (13 years, 8 months ago)

From my experiences from school, that is the basic and perhaps the worst way to get anything, it's slow painful and weak. Adding hormones to your culture is the best way.


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Re: Yello stuff on plant Tissue culture (Agar) [Re: neuro]
    #1415738 - 03/28/03 12:25 AM (13 years, 8 months ago)

i've got some rooting hormone(for making cuttings) but i think its the wrong kind for tissue culture, most other hormones are hard to get here in holland


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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1415823 - 03/28/03 10:50 AM (13 years, 8 months ago)

Well there isn't really a wrong kind, you just need semi-pure compounds and mix up the complimentary concentrations for what you wish to get.


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Re: Yello stuff on plant Tissue culture (Agar) [Re: Anand]
    #1415936 - 03/28/03 02:22 PM (13 years, 8 months ago)

When you order from amazon, 8$ shipping adds to the price of the book, so it would be 25?.

If you want, I could order it for you and you send me the money.


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Amazon Shop for: ½ Pint Jars, Agar, Salvia

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