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Amazon Shop: Agar, HEPA Filter, Laminar Flow Hood

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Invisiblenewuser1492
Registered: 06/12/03
Posts: 3,104
Glove box vs. Flow hood vs. Pressure box
    #3824512 - 02/23/05 10:47 PM (13 years, 5 days ago)

I'm looking to create a sterile environment for agar, spore prints, syringes .. etc.

The glove box looks to be about the cheapest and easiest to build but I don't see how it creates a sterile environment. You would have to open it up to put items in and then all the dirty air would be trapped inside. Would spraying lysol or oust work? I was figuring you could spray lysol or oust and let it sit for 10 minutes then start working. Would the fumes from the lysol or oust still kill spores or fungus?

If that doesn't work I was looking at a flow hood or pressure box. It seems that a pressure box would afford an environment more suitable to sterility than a flow hood.

Anyone have any suggestions?


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InvisibleHolydiver
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Re: Glove box vs. Flow hood vs. Pressure box [Re: newuser1492]
    #3824559 - 02/23/05 10:55 PM (13 years, 5 days ago)

The idea of the basic glove box (basic=sans HEPA + fan) isn't to create a sterile environment, it simply gives you a draft free area to work in, preventing the bad guys from landing on your work surface via air current, breathing, etc.

You could put a can of Lysol in the work area with all your stuff, and spray everything down, then do your work from there--that would help alot. Waiting a few minutes for the lysol to work and letting everything settle down never hurts.

For me, the simple glove box always worked great. I've also done transfers/syringes/inoculations in clear trash bags, large ziplock bags...the idea is to prevent contams from landing on your work area.


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Offlinehyphae
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Re: Glove box vs. Flow hood vs. Pressure box [Re: Holydiver]
    #3824775 - 02/23/05 11:30 PM (13 years, 4 days ago)

A simple glove box will give you damn close to 95-100% results as long as your sterile culture technique is not flawed :wink: GL


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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineVALIS
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Re: Glove box vs. Flow hood vs. Pressure box [Re: newuser1492]
    #3825101 - 02/24/05 12:25 AM (13 years, 4 days ago)



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Nature is the Technology of the Divine.


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Amazon Shop: Agar, HEPA Filter, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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