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psh
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very slow, clear growth on agar *DELETED*
#27297226 - 05/06/21 03:37 PM (2 years, 10 months ago) |
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Post deleted by psh
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tiptrippy
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Re: very slow, clear growth on agar [Re: psh]
#27297231 - 05/06/21 03:42 PM (2 years, 10 months ago) |
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Not sure really but I would try another batch of agar.
500ml distilled water 10g agar Whatever nutes you want
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sandman420
Saint PP
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Re: very slow, clear growth on agar [Re: tiptrippy]
#27297235 - 05/06/21 03:46 PM (2 years, 10 months ago) |
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Yea i think it has too much sugar
edit 118ml of water/1g sugar is too low
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Ashtray161
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Re: very slow, clear growth on agar [Re: sandman420]
#27297238 - 05/06/21 03:50 PM (2 years, 10 months ago) |
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ive been getting clear growth like that too on a couple of my APE plates but also mycl
-------------------- (You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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tiptrippy
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Re: very slow, clear growth on agar [Re: Ashtray161]
#27297244 - 05/06/21 03:54 PM (2 years, 10 months ago) |
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That mycelium is probably healthy, just growing THROUGH the agar instead of on top of it.
Make a new batch of agar and you can probably just transfer that clear sample to a new dish and I have a feeling it will look normal once it grows out.
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Feasoghorm
Registered: 10/24/18
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Re: very slow, clear growth on agar [Re: tiptrippy]
#27297396 - 05/06/21 05:58 PM (2 years, 10 months ago) |
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Looks like patches of germinating spores.
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Ashtray161
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Re: very slow, clear growth on agar [Re: tiptrippy]
#27297486 - 05/06/21 07:02 PM (2 years, 10 months ago) |
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Quote:
tiptrippy said: That mycelium is probably healthy, just growing THROUGH the agar instead of on top of it.
Make a new batch of agar and you can probably just transfer that clear sample to a new dish and I have a feeling it will look normal once it grows out.
How does that end up happening? 0.o Im also curious on nutrients and what the best recipe is. So far I have agar and "Light Dried Malt Extract" on the way.. Is there any other ingredients I should be ordering? Im still very confused on the actual agar ingredients and amounts because the thread on agar recipes assumes you understand a bunch acronyms which I dont and doesnt make it too easy to tell how much of what to put in there this is the thread im referencing: https://www.shroomery.org/forums/showflat.php/Number/18676888
-------------------- (You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
Edited by Ashtray161 (05/06/21 07:03 PM)
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Ashtray161
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Re: very slow, clear growth on agar [Re: Ashtray161]
#27297488 - 05/06/21 07:05 PM (2 years, 10 months ago) |
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"1) Malt-extract: 10g LME(a) / GLME (b), 10g agar
Pictured here is GLMEA, it works great, pretty much the standard I judge media against."
Like this is what im trying to make but im completely lost as to what the ingredient list is
-------------------- (You Know What Time It Is) Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086 "You never have to prove the fool a fool, just let them speak." Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED. Be Gay, Do Crime 161 1312
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tiptrippy
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Re: very slow, clear growth on agar [Re: Ashtray161]
#27297532 - 05/06/21 07:31 PM (2 years, 10 months ago) |
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Quote:
Ashtray161 said: "1) Malt-extract: 10g LME(a) / GLME (b), 10g agar
Pictured here is GLMEA, it works great, pretty much the standard I judge media against."
Like this is what im trying to make but im completely lost as to what the ingredient list is
Very simple recipe using the ingredients I linked to you in the other thread.
500ml distilled water 10g agar 7g LME
PDA potato dextrose agar Anytime you see Y it usually means yeast or nutritional yeast GSW is grain soak water. (Use the water that you soaked grains in. Diluted of course)
LME light malt extract DLME dried light malt extract ELME extra light
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LadysKnight
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Re: very slow, clear growth on agar [Re: tiptrippy]
#27298152 - 05/07/21 06:55 AM (2 years, 10 months ago) |
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If you do some transfers from this plate to new ones, I'm hoping you'll keep this plate to see what it becomes.
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psh
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Re: very slow, clear growth on agar *DELETED* [Re: LadysKnight]
#27300689 - 05/08/21 07:35 PM (2 years, 10 months ago) |
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Post deleted by psh
Reason for deletion: .
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tiptrippy
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Re: very slow, clear growth on agar [Re: psh]
#27300692 - 05/08/21 07:38 PM (2 years, 10 months ago) |
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That's better. Did you end up making a new batch of agar? If so, what was the new recipe you went with?
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psh
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Re: very slow, clear growth on agar *DELETED* [Re: tiptrippy]
#27300697 - 05/08/21 07:42 PM (2 years, 10 months ago) |
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Post deleted by psh
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Benson
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Re: very slow, clear growth on agar [Re: psh]
#27300814 - 05/08/21 09:19 PM (2 years, 10 months ago) |
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That does not seem like enough neuts. The PDYA recipe in Frank's recipe book that he says is his favorite recipe (at the time, at least) was:
10g agar 10g potatoe 7g dextrose 1g nutritional yeast 500ml water
Another way to look at it is % neuts using the ml of water. So 5g potato flakes is only 1% neuts and most people use 2-3%.
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psh
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Re: very slow, clear growth on agar *DELETED* [Re: Benson]
#27300856 - 05/08/21 09:53 PM (2 years, 10 months ago) |
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Post deleted by psh
Reason for deletion: .
Edited by psh (05/08/21 09:55 PM)
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Julius orange
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Re: very slow, clear growth on agar [Re: psh]
#27300915 - 05/08/21 10:56 PM (2 years, 10 months ago) |
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Sometimes it's your recipe. Sometimes it just be like that. Especially with germ plates. Just transfer until clean. I've made grain water agar that would get growth like this. I cleaned the cultures and put them to grain. They thickened up in the jars and fruited just fine. Pretty plates are pleasant but not the end game.
Of course with consistent looking mycelium on agar. It is easier to see contamination and easier to see sectoring.
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Benson
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Re: very slow, clear growth on agar [Re: psh]
#27300923 - 05/08/21 11:05 PM (2 years, 10 months ago) |
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Quote:
psh said: I need to do some reading on this, haha. Don't have any yeast or dextrose atm. Thanks, maybe I'll try this recipe tonight, and change it up if it doesn't work out.
500 ml water 10g potato flakes 10g agar a few drops of honey or corn syrup
That sounds like it'll probably get the job done
Dextrose is kinda a pain in the ass, I couldn't find anything less than a pound of it and all I need is 7g at a time lol
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sandman420
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Re: very slow, clear growth on agar [Re: Benson]
#27301097 - 05/09/21 04:59 AM (2 years, 10 months ago) |
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not a few drops of honey, with that recipe you need 10 grams of sugars for regular PDA recipe...
a few drops of honey you be right where you started with too low sugar ratio
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psh
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Re: very slow, clear growth on agar *DELETED* [Re: sandman420]
#27301606 - 05/09/21 12:44 PM (2 years, 10 months ago) |
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Post deleted by psh
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Stipe-n Cap
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Re: very slow, clear growth on agar [Re: psh]
#27301636 - 05/09/21 01:20 PM (2 years, 10 months ago) |
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This happens to me from time to time with germ plates. You may even notice this kind of growth forming what looks like a halo around the perimeter of your culture, it's just mycelium.
I always use the standard recipe of 10gr agar, 10gr LME 500ml h20, and then double it.
I'm not sure that it's much more than that, your mycelium just hasn't "filled in" yet. People may say that softer agar would be better for germ, but this happens infrequently ime.
If you transfer that growth to a new plate you'll have no issue, so long as it's clean.
Edit:
This happens every time I've ever tried to use grain soak water for nutes.
Edited by Stipe-n Cap (05/09/21 01:32 PM)
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psh
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Re: very slow, clear growth on agar *DELETED* [Re: Stipe-n Cap]
#27313792 - 05/18/21 12:52 PM (2 years, 9 months ago) |
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Post deleted by psh
Reason for deletion: .
Edited by psh (05/18/21 12:56 PM)
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psh
Stranger
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Re: very slow, clear growth on agar *DELETED* [Re: psh]
#27317640 - 05/21/21 02:10 PM (2 years, 9 months ago) |
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Post deleted by psh
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Mycelium Juice
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Re: very slow, clear growth on agar [Re: psh]
#27317666 - 05/21/21 02:47 PM (2 years, 9 months ago) |
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The turning yellow might just be the mycelium eating away the colored dye used for the plates. Depends on if it's on the actual mycelium or not I guess. Check the bottom of the plate, if it's losing coloring that's prbly all it is. Kind of looks like that's the case in pic 3 and 6.
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