I got my eye out for a good method to go from spore print to liquid mycellium (honey or malt). This supposedly could be used to innoculate honey/malt mixture from mushroom tissue?

What would you do different if you had a spore syringe, just scrape some spores into the h202 jar?

thanks.

There are 3 major things you need to know about H2O2:

1. It gets broken down by mushroom/mycelial tissue when the cell walls are breached
2. Most growth medium also breaks it down unless heat treated.
3. It kills spores!

So if you put it in contact with spore prints or spore water then you will just kill the spores.

IMHO the recent enthusiasm about it's use in shroom growing is mostly from people who have not actually tried to use it. It has severe limitations.

I have developed a method which uses it at every stage, including in the actual growth medium. This virtually eliminates all contamination and means that you can colonise your substrate in the same trays that you fruit in. You have to use pressure cooked grain (wheat or rye) to prevent the H202 being degraded or you can use Weetabix (spun, whole grain pillows) as this is unreactive.

Having got it to work I have returned to using straight-forward agar in a ?5.00 home-made glovebox. It works out far simpler and quicker.

Rush Wayne's book on the subject has some methods which look promising for wood loving shrooms. It uses compressed-paper fuel pellets. He also have a recipe for H2O2 agar which is excellent for cloning.

Rad

that would be cluB99.

peace
hip

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[This message has been edited by Hippie3 (edited April 11, 2000).]

What do you do with the rice/water mixture after the mycelum grows?

Thanks.

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Away!

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Away!

Look, agar is just plain EASY, ok? PF tek only made it harder to teach people how to actually culture sterile mycelium properly.

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First agar..

Choose your agar mixture (there are hundreds of recipes to choose from) and prepare it. You can get premade mixtures, or mix your own.
note: this involves boiling all your ingrediants, sometimes straining out solids (depending on the recipe). You will need containers to do the agar work in, typically petri dishes, but wide mouth jars will work.. If you use plastic petri dishes, you must sterilize your agar mixture in a sperate container and somehow manage to pour it into your dishes, WHILE HOT! You must do this in a sterile location and keep them in a sterile location so they don't suck in dirty air while they cool.

Then once they solidify you just innoculate them, either by isolating a strain from another agar dish, cutting it out (hoping you have selected a single strain) and transfering it (in open air again) into the new fresh petri dish.

Of you can skip right to the culturing of a fresh mushroom to isolate a strain. This involes slicing open a stem and cutting out a section of the inside mycellium, and transfering to the fresh dish (all while praying your scalpel is clean and that nothing gets in your dish while it is open, or lands this clean piece of mycellium while you transfer it, or that you didn't contaminae it by touching the outside of the mushroom with ANYTHING once you sliced open the mushroom)...

And now you still have to wait until the dish grows out until you can use it. If there is contamination you have to keep trying to reisolate your strain from this dish into another dish. Then if you have managed to do this without contaminating it, you still have to take a risk again when you use this stuff to innoculate your substrate... either by usuing wedges (and openeing your substrate jars wide open) or blending it up.. (another open container)...
time... minimum 10 days... practically... 14-21 days.

Ok and now the new peroxide method..

Put piece of stem in coffee grinder with water or h2o2... No sterile conditions necessary...

Blend....No sterile conditions necessary...

Spoon into 1/3 cup peroxide...no steil conditions necessary...

Suck up with syring... No sterile conditions necessary...

Innoculate... time 5 minutes...

Yeah thats the same...

As a side note:

I do have quart jars of birdseed with all three of those species growing,(shitake, crimini, chantarelles) although I did make one stupid mistake... I bothered with the Chantarelles... the mycellium is growing, but I am pretty sure they can't be cultivated... I didn't realize this when I started.. Oh well... I will try anyway for the fun of it..

ThE JafF