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OfflineFGL
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Registered: 06/25/05
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Re: First AGAR atempt log... [Re: veda_sticks]
    #7575517 - 10/30/07 06:23 AM (16 years, 3 months ago)

sorry man...have you read my questions? what aam i doing fine so far?:rolleyes:


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Offlineveda_sticks
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Re: First AGAR atempt log... [Re: FGL]
    #7575610 - 10/30/07 07:21 AM (16 years, 3 months ago)

lol, just commenting that while u have questions and a few problems it looks like your doing a not to bad job so far.

If it where my first attempt, i probably be throwing petri dishes of green mold away.


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OfflineRogerRabbitM
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Re: First AGAR atempt log... [Re: veda_sticks]
    #7575625 - 10/30/07 07:32 AM (16 years, 3 months ago)

FGL
Hot MEA with Gentmycin sulphate as antibiotic is what I use. Normally, I let the agar cool until I can hold the bottle to pour without any heat protection for my hands. If I'm trying to pour over bacterial contamination, I'll pour it a bit hotter. The idea is to knock the bacteria back with heat, but the stronger mushroom mycelium will survive the hot agar to grow up through to the top.
RR


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OfflineFGL
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Registered: 06/25/05
Posts: 572
Last seen: 9 years, 9 months
Re: First AGAR atempt log... [Re: veda_sticks]
    #7575648 - 10/30/07 07:57 AM (16 years, 3 months ago)

Quote:

veda_sticks said:
lol, just commenting that while u have questions and a few problems it looks like your doing a not to bad job so far.

If it where my first attempt, i probably be throwing petri dishes of green mold away.




Ahhh:grin: but this is not my first attempt but MushroomJoe (the original poster) first attempt...:tongue::grin:


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OfflineFGL
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Posts: 572
Last seen: 9 years, 9 months
Re: First AGAR atempt log... [Re: RogerRabbit]
    #7575652 - 10/30/07 07:59 AM (16 years, 3 months ago)

Quote:

RogerRabbit said:
FGL
Hot MEA with Gentmycin sulphate as antibiotic is what I use.  Normally, I let the agar cool until I can hold the bottle to pour without any heat protection for my hands. If I'm trying to pour over bacterial contamination, I'll pour it a bit hotter.  The idea is to knock the bacteria back with heat, but the stronger mushroom mycelium will survive the hot agar to grow up through to the top.
RR




Perfect!:thumbup:
Many thanks for the answer RR!;):thumbup::thumbup::thumbup:


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