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Re: AGAR ENVY! (Anything and All things agar!) [Re: 999999999] * 2
    #28027263 -

Gleyck said:

Germination plate that I probably left a bit too long waiting for T1. Probably 6 oโ€™clock is the best bet?

Also: considering that this plate is clean, what are the advantages and disadvantages of sending it to grain as is?
From what I understood, and correct me if Iโ€™m wrong: if the purpose is a potential clone selection, one would want the maximum genetic variability in the tub, so a T1 is already detrimental for that goal by narrowing the gene pool with a questionable criteria, behaviour on agarโ€ฆ ?



Welcome.

You're missing the point. If you're asking what I think you're asking, you're not looking for a genetic clone with a T1. You're looking to get good strong mycelium and clean it up. Make sure there's not contam before sending to grain. Personally I'd take another small bit from that, grow out in another plate, then send a one inch-square of it to PCed grain.

6 oclock would work, so would 8 oclock or the top of that area. Any of the ends that look like roots or lightning. Take the smallest bit you can, less chance of transferring anything nasty. 

To get a good clone culture, YOU FIRST GROW SOME SHROOMS, AND EAT SOME to try them out.

Or at least pick the one that's fattest, tallest, first, healthiest, grows quickly, grows clusters, or any other things you want to have in your clone. THEN you clone that.

Grow some shrooms, pick a good one, and clone it. Pick a few good ones and clone them, grow a shoebox of each, try a bit of each, then clone those.

I see this a lot on here, and I can see how it would be an easy mistake to make.

Also, if you get a plin (plate pin), clone that. That's how I got my favorite clone, and it's mostly what I grow. Your mileage may vary, but it's an easy way to clone. Clone a plin before any other plins drop spores, and cut off above the base so you don't bring any non-clone mycelium along to your clone plate.

Also, don't fall into the trap here of trying to isolate selections of clones down to single threads. A good clone culture can have a few different strands in it, and still produce consistently good, consistent shrooms for many tubs for a long time.

If you do isolate selections of clones down to single threads, it will get pissed and start throwing up new genetic lines. Someone here did plate transfers to like T100 and still couldn't get it to one type.

Multispore (non-clone, growing from a print) has a lot of genetic variety, and grows are inconsistent. Clones, even if they have a few genetic variations in it, are far more consistent.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28027318 -

nektar61 said:
Rotnpins said:
thehiveatp said:
Rotnpins said:
The spot in the center is bacteria.. the one at the top, idk.. it's still early. It could be cube mycelium, but I'd give it a little more time and check back

Can you get a clearer picture of the spot at the top?



This is the best I managed.. gotta figure out the technique for shooting these things, glares galore. It's more textured than pictured, but still definitely has that glossy dome look the bacteria does.





Still really hard to see.. but they picture looks less like mycelium.

There is a good write-up about how to take good pictures of plates.. I'll see if I can find it and edit in the link for you

Edit: I can never seem to find the thread :shrug: it should be in my favorites.. but it's not. . Maybe someone else knows the thread I'm talking about and can share the link with you



I have this:
Taking better Petri dish and shroom grow photos:
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27726375&page=0&vc=1#27726375

That will tell you how to take a pic that's not blurry.

But I think the dot in near the top of that plate pic is bacteria. It's shiny, it's reflecting a little light.

Cube mycelium isn't shiny. Bacteria often is.

Anything I've written here that's really worth reading is linked in the article linked in my sig line called,
"New? Things I wish I'd known when I started here."





Thats funny.. I immediately thought of your write up when I was looking for it :lol: I scanned through the links like 3 times and didn't see it

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #28027629 -

y'all are too kind! thank you for the confirmation. g

onna go dig into those two threads now.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: thehiveatp]
    #28028327 -

How soft should one make agar for spore germination?

Last time i swabbed some plates zero germinated , so this time im guna make my agar nice and wet. Just wondering what ratio of MEA to water would be appropriate ?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Luminous7]
    #28028335 -

7g agar to 500ml

Can do similar ME. You decide.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #28028455 -

That will cook into a solid gel? Seems like it will be soup lol

I use a premixed MEA from sea weed labs and they recommend, 27.7g agar per 500ml


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Luminous7]
    #28028460 -

Luminous7 said:
That will cook into a solid gel? Seems like it will be soup lol

I use a premixed MEA from sea weed labs and they recommend, 27.7g agar per 500ml



That is super firm. For regular agar, people will use 10g per 500ml of water. For softer agar about 7g is a good space. 27ish grams of agar for half a liter of water is going to be mega firm.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Luminous7]
    #28028464 -

Luminous7 said:
That will cook into a solid gel? Seems like it will be soup lol

I use a premixed MEA from sea weed labs and they recommend, 27.7g agar per 500ml



That's probably 10g agar agar, 10g malt extract and w/e else.

Not all agar is created equal btw. When I was using freeze dried blocks 10g wouldn't even fully disolve in 500ml. 8 was the most I could do.

Judging by how stiff my current agar turns out at 10g for 500-600ml, I'm pretty sure I could go down to 7 and still solidify.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28028480 -

I used that sea weed solution premix and was not happy with it


--------------------

Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Luminous7]
    #28028481 -


Hello, above is a picture of a t1 or 2 of a pin. My growth has been unusual lately mainly looking like the 12 and 5 oโ€™ clock position. The mycelium looks wet and doesnโ€™t have the normal characteristics Iโ€™m used too. Iโ€™m assuming itโ€™s a contam but thought I should ask because it doesnโ€™t resemble any contam Iโ€™ve seen before or the ones on the plate shown.
    My plates are made with 5g malt extract, 5g agar and .5g yeast.
https://www.shroomery.org/forums/showflat.php/Number/26828142

  Iโ€™ve also included this thread that shows another good picture of somebody elseโ€™s plate that looks similar to my growth. Hoping that Adding this will help someone troubleshoot this with me.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dorfnob]
    #28028499 -

Dorfnob said:

Hello, above is a picture of a t1 or 2 of a pin. My growth has been unusual lately mainly looking like the 12 and 5 oโ€™ clock position. The mycelium looks wet and doesnโ€™t have the normal characteristics Iโ€™m used too. Iโ€™m assuming itโ€™s a contam but thought I should ask because it doesnโ€™t resemble any contam Iโ€™ve seen before or the ones on the plate shown.
    My plates are made with 5g malt extract, 5g agar and .5g yeast.
https://www.shroomery.org/forums/showflat.php/Number/26828142

  Iโ€™ve also included this thread that shows another good picture of somebody elseโ€™s plate that looks similar to my growth. Hoping that Adding this will help someone troubleshoot this with me.



That some 1 day late Halloween myc?

You using colorant?


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dorfnob] * 3
    #28028501 -


ESS revival project progress. The plates labled T3 and T2 are already in Muda bottles. The T1s will be going in ASAP.


Ps natalensis from a 3 month old plate, just snagged a bit of the aerial/overlay myc. No actual agar, it was a potato chip at that point


More 2 and 3 month old plates rescued. Ps subtropicalis, Omni (brain/blob mutation from Melmak TP), Squatknots, Ps hispanica and an Avery's Albino clone


Ps zapotecorum swab germination


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 1
    #28028543 -

Never had a dish of pan Cambodian pin till recently. I had around 20 little pins growing out of one spot and I couldnโ€™t resist putting them to agar

Iโ€™m assuming everything that grows from a pin will be a clone of that pin right?
If I have 20 pins on dishes, I will have 20 different clones, correct ?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28028549 -

Yes it does have food coloring in it, which I thinks expired because it doesnโ€™t produce a nice color like it used to no matter how much I put in.and yeah itโ€™s pretty scary looking. You think the food dye has something to do with it?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dorfnob]
    #28028556 -

Dorfnob said:
Yes it does have food coloring in it, which I thinks expired because it doesnโ€™t produce a nice color like it used to no matter how much I put in.and yeah itโ€™s pretty scary looking. You think the food dye has something to do with it?



You used any red?


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28028561 -

Rusty2096 said:
Dorfnob said:
Yes it does have food coloring in it, which I thinks expired because it doesnโ€™t produce a nice color like it used to no matter how much I put in.and yeah itโ€™s pretty scary looking. You think the food dye has something to do with it?



You used any red?



Yes, I do. What of it?


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 1
    #28028563 -

SirPsycho said:
Rusty2096 said:
Dorfnob said:
Yes it does have food coloring in it, which I thinks expired because it doesnโ€™t produce a nice color like it used to no matter how much I put in.and yeah itโ€™s pretty scary looking. You think the food dye has something to do with it?



You used any red?



Yes, I do. What of it?



:jimmies:


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28028571 -

SirPsycho said:
Rusty2096 said:
Dorfnob said:
Yes it does have food coloring in it, which I thinks expired because it doesnโ€™t produce a nice color like it used to no matter how much I put in.and yeah itโ€™s pretty scary looking. You think the food dye has something to do with it?



You used any red?



Yes, I do. What of it?



Can you please be serious for a minute and not bring up your lipstick in every conversation?


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096] * 1
    #28028584 -

Rusty2096 said:
SirPsycho said:
Rusty2096 said:
Dorfnob said:
Yes it does have food coloring in it, which I thinks expired because it doesnโ€™t produce a nice color like it used to no matter how much I put in.and yeah itโ€™s pretty scary looking. You think the food dye has something to do with it?



You used any red?



Yes, I do. What of it?



Can you please be serious for a minute and not bring up your lipstick in every conversation?





--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28028641 -

Maybe but I donโ€™t think so I feel like I used green. Usually my plates have a brownish tint from the malt.

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