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Melliferous
Σκωλοτοι

Registered: 10/01/20
Posts: 1,060

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27201036 - 02/12/21 08:06 AM (5 years, 6 months ago)

D3monic said:

Yea ... I 1g per 100ml but honestly I don’t measure. I just do like half a spoon scoop full 🤣 but peeps wanted exact measurements so I weighed a scoop and went with the average for the tek. I’m never that “exact” with it personally.

Is there an advantage to using the charcoal in the mix? I searched a little and read some RR notes on the subject about healthier myc and less bacterial contams? Is there anything else going on?

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Melliferous]

1
#27201046 - 02/12/21 08:12 AM (5 years, 6 months ago)

Not that I’ve seen. Just a color contrast without any additional nutrients added to the plate.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Melliferous
Σκωλοτοι

Registered: 10/01/20
Posts: 1,060

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27201052 - 02/12/21 08:18 AM (5 years, 6 months ago)

D3monic said:

Not that I’ve seen. Just a color contrast without any additional nutrients added to the plate.

I might give it a try. I've read that mycorrhizal fungi love charcoal and seem to develop more prolifically when it's present in the soil. It serves as a habitat/nutrient storage location for them -- I suppose the same would happen in a petri, but I'd be interested to see if all the carbon particles would act like a grain and be another inoculation source?

Edited by Melliferous (02/16/21 06:27 PM)

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Melliferous]

1
#27201091 - 02/12/21 08:48 AM (5 years, 6 months ago)

Melliferous said:

D3monic said:

Not that I’ve seen. Just a color contrast without any additional nutrients added to the plate.

I might give it a try. I've read that mycorrhizal fungi love charcoal and seem to develop more prolifically when it's present in the soil. It serves as a habit/nutrient storage location for them -- I suppose the same would happen in a petri, but I'd be interested to see if all the carbon particles would act like a grain and be another inoculation source?

I did a little experimenting which included the black. https://www.shroomery.org/forums/showflat.php/Number/27003803#27003803

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Melliferous
Σκωλοτοι

Registered: 10/01/20
Posts: 1,060

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27201160 - 02/12/21 09:33 AM (5 years, 6 months ago)

Thanks for the link D3, I will definitely try out the charcoal with the next batch of plates. :thumbup:

:thumbup:

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Tucky
Smart ass.

Registered: 12/13/20
Posts: 139
Loc: Near a tree by a lake

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27201181 - 02/12/21 09:47 AM (5 years, 6 months ago)

So I found some premade agar petri dishes with malt extract agar in my surfing of the google box and ordered a few. Am I going to have to pressure cook these when they show up or will that fuck em up? I'm new to agar and these seemed cheap enough so if this is something that I should not use I wont be out much.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tucky]

1
#27201189 - 02/12/21 09:50 AM (5 years, 6 months ago)

If they are premade I’d hope they was sterilized.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Tucky
Smart ass.

Registered: 12/13/20
Posts: 139
Loc: Near a tree by a lake

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27201200 - 02/12/21 09:56 AM (5 years, 6 months ago)

D3monic said:

If they are premade I’d hope they was sterilized.

You and me both. Ill contact the manufacturer and ask them. I'll also inspect the packaging really well before I do anything with them

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TheDoobsker
Amateur

Registered: 05/22/20
Posts: 815

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27201567 - 02/12/21 12:46 PM (5 years, 6 months ago)

D3monic said:

It’s a custom made tool with brass tubing and an acrylic handle.

I’m in the process of trying to Aquire another but the gentleman that made it shop isn’t heated so will be a few months before that happens. Wanted to use it in a contest/giveaway

Just wanted to add my input to this for anyone wanting a similar tool.
I started using a metal straw only for my clean cultures, inspired by D3

In high school I used to take a small screwdriver, get the metal part red hot and pull it out of the plastic. Then I'd take my honda civic keys and do the same and transfer it into the screwdriver handle. The result is cleaner than you'd expect

I'm sure this could be done with a metal straw as well. just cut it down to a few inches and I'd think you'd have a nearly identical tool

--------------------

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clockworkshroom
Stranger

Registered: 02/20/20
Posts: 689
Last seen: 6 months, 30 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tucky]
#27201613 - 02/12/21 01:01 PM (5 years, 6 months ago)

Drunken Lullabies1 said:

D3monic said:

If they are premade I’d hope they was sterilized.

You and me both. Ill contact the manufacturer and ask them. I'll also inspect the packaging really well before I do anything with them

They won't sell them non sterile so will only answer yes to you, definitely no need to pressure cook yourself. The question is whether they sterlised them properly. You are definitely best off doing it yourself if you have a pressure cooker.

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p9hu7's clean spawn thread

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Gastronomicus
3-0-G

Registered: 03/31/05
Posts: 9,746
Last seen: 1 month, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: clockworkshroom]
#27201866 - 02/12/21 02:54 PM (5 years, 6 months ago)

Finnnnaaaaallly found some LME. Lets see if I can get that elusive ryzo growth now

--------------------
Make my Funk the P Funk, I wants to get Funked up

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PitcherCrab
Crescent Fresh

Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 7 months, 21 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: TheDoobsker]
#27201877 - 02/12/21 03:00 PM (5 years, 6 months ago)

TheDoobsker said:

D3monic said:

It’s a custom made tool with brass tubing and an acrylic handle.

I’m in the process of trying to Aquire another but the gentleman that made it shop isn’t heated so will be a few months before that happens. Wanted to use it in a contest/giveaway

Just wanted to add my input to this for anyone wanting a similar tool.
I started using a metal straw only for my clean cultures, inspired by D3

In high school I used to take a small screwdriver, get the metal part red hot and pull it out of the plastic. Then I'd take my honda civic keys and do the same and transfer it into the screwdriver handle. The result is cleaner than you'd expect

I'm sure this could be done with a metal straw as well. just cut it down to a few inches and I'd think you'd have a nearly identical tool

Yup! I did almost exactly that with some brass 1/4" tubing and 2 part epoxy puddy and made this lil guy:

--------------------
PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear

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Nichrome
Skank

Registered: 12/17/18
Posts: 8,899

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PitcherCrab]
#27201901 - 02/12/21 03:10 PM (5 years, 6 months ago)

I'd like to se one with an angle for personal reasons... :shrug:

:shrug:

--------------------
Question everything, it's very responsive.
culture storage

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TheDoobsker
Amateur

Registered: 05/22/20
Posts: 815

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27201945 - 02/12/21 03:26 PM (5 years, 6 months ago)

Nichrome said:

I'd like to se one with an angle for personal reasons...

Metal straws usually have an angle

Big thanks to Quirk for the MM swabs and Ambrose for the ATL#7 prints

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 9 hours, 23 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: TheDoobsker]

1
#27203003 - 02/13/21 04:37 AM (5 years, 6 months ago)

Haven’t seen this in a while.

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27203010 - 02/13/21 04:44 AM (5 years, 6 months ago)

Whenever I used to have something like that it was always 100% meshed-in mold, had me hella puzzled for a while the times that happened.
Might be about the most difficult contam to get rid of. Some people have had success with a hot-pour and I had success once poking it and transferring to pucks, the mold and the cube myc separated themselves and I could save the culture.

--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 9 hours, 23 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]
#27203048 - 02/13/21 05:21 AM (5 years, 6 months ago)

Yeah if the t3s I took a few days ago do it I’m gonna try poking them next.

Last year pesa kept doing this and I eventually ran it but it took like five weeks to pin.

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King87
Stranger

Registered: 06/04/16
Posts: 60
Last seen: 3 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27203118 - 02/13/21 06:49 AM (5 years, 6 months ago)

Total noob here, trying to go from print to agar. I have PC'd the q tip for 20 mins and left it in the jar, the paper towel has ISO on there. Any feedback for improving technique is welcomed!

Edited by King87 (02/13/21 01:53 PM)

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Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: King87]
#27203130 - 02/13/21 06:59 AM (5 years, 6 months ago)

~~Upload the picture here.~~ People don't like to open outside links.

Just realized that it was a video. I'm not a fan of swabs. You can make an inoculation loop with a piece of wire and an Xacto Knife handle.

When you PC tools, it's better to wrap them in aluminum foil. That way you can carefully unwrap the tool vs fidgeting with a big jar and you Definitely don't want to be reaching over anything that you want to keep sterile. Shirt sleeves are good at dropping contams all over the place and you want to use slow movements inside the box.

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Edited by Doctor Mario (02/13/21 07:22 AM)

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King87
Stranger

Registered: 06/04/16
Posts: 60
Last seen: 3 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27203145 - 02/13/21 07:10 AM (5 years, 6 months ago)

Doctor Mario said:

Upload the picture here. People don't like to open outside links.

It's a vid, cant seem to embed it properly.

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Shop:

Isolated Cubensis Liquid Culture For Sale

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