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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #27199842 -



Does this look infected? Lol

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27199876 -

The yellow parts look a bit infected, but you've got a lot of myc free from contam to xfer!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycanthrope]
    #27199918 -

Pretty cool looking, even if it is contamed...I love all the crazy myc that comes off plate pins.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #27200044 -

D3monic said:
I’m in the process of trying to Aquire another but the gentleman that made it shop isn’t heated so will be a few months before that happens.



Heated? Like he needs to be pissed off to make these? :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27200055 -

D3monic said:
clockworkshroom said:
I saw you've evolved to a metal straw or something now, I was wondering about how long it takes to cool a punch. I'll try it soon



It’s a custom made tool with brass tubing and an acrylic handle.





What’s the diameter?



8mm
$8.49
Handle wrapped in silicone fusion tape.(not sure it’s even necessary)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #27200060 -

Lmao, he lives in maine or some place cold as hell.

I ordered some 8mm tubing to try and make my own when I got free time. It was bigger than my tools so maybe 7-7.5 mm

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27200177 -

D3monic said:
It’s a custom made tool with brass tubing and an acrylic handle.






Cool!

how do you get the punched agar / mycelium core sample out of there?

Does it just fall out into the transfer plate, or do you have to tap the tube with something?

Edit: Now I see there's a chamber in the side. Do you poke something through there to push out the agar plug?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27200284 -

nektar61 said:
D3monic said:
It’s a custom made tool with brass tubing and an acrylic handle.






Cool!

how do you get the punched agar / mycelium core sample out of there?

Does it just fall out into the transfer plate, or do you have to tap the tube with something?

Edit: Now I see there's a chamber in the side. Do you poke something through there to push out the agar plug?



Click the agar link in my sig.

It’s more like a cookie cutter, the agar wedge stays on its plate. You use your scalpel to lift it to the new plate.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27200292 -

Anyone else do dumb shit like this? No this is not super clear agar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ModularMind]
    #27200298 -

ModularMind said:
What’s the diameter?



8mm
$8.49
Handle wrapped in silicone fusion tape.(not sure it’s even necessary)




Got a link for that? Been looking for something cheap that's right angled like that so I don't have my hand over the plate. Looks kind of thick though how fast does it cool?


--------------------
:chems:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheBoJim]
    #27200308 -

TheBoJim said:
Anyone else do dumb shit like this? No this is not super clear agar.




:lol:

Is that a transfer to an empty petri or something else? Is there a goal or is it just for shits and giggles?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ambrose] * 1
    #27200347 -

Didn’t realize I had an empty dish in a stack  :foreheadslap:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lenz]
    #27200370 -

Lenz said:
ModularMind said:
What’s the diameter?



8mm
$8.49
Handle wrapped in silicone fusion tape.(not sure it’s even necessary)




Got a link for that? Been looking for something cheap that's right angled like that so I don't have my hand over the plate. Looks kind of thick though how fast does it cool?



Dental Tissue Punch Angled 8mm...on the a8==Dz website

Edit: I’ve only used a 4mm version who’s walls are much thicker and it is difficult to cool. These walls are thinner. I’ve yet to use it though. I’m sure it will work for my purposes.

Edited by ModularMind (02/11/21 09:11 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheBoJim]
    #27200441 -

TheBoJim said:
Didn’t realize I had an empty dish in a stack  :foreheadslap:



Aaah I see. No pouring noob here so I didn't even think of that :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycanthrope]
    #27200844 -

Mycanthrope said:
Big day tuesday! I've got 11 agar plates active right now, 3 clones from my malabar grow, 3 transfers from melmac germ plates (thanks Qirk!), and 6 germ plates with spores from Roar!

The melmac transfers:



The malabar clones (rather bacterial I know, but one I can transfer away and the other looks clean so it's a success in my book!):



All the plates together:






Those lids says PET1. I couldn't of sterilized them so have do you go about this?:takingnotes:

Edited by Puduwoke (02/12/21 04:16 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheBoJim] * 1
    #27200856 -

TheBoJim said:
Didn’t realize I had an empty dish in a stack  :foreheadslap:



Ah taking no pour to a new level! Let us know...  :lol:

Today I transfered 12 wedges, a block of 4 tiny slices from the same spot, three times for three plates.
All from T3 meh crappy looking APE. I'm determined to stop cultivating contam! :awedance:


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Edited by Inthepit (02/12/21 05:42 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Puduwoke]
    #27200945 -

Puduwoke said:
Mycanthrope said:
Big day tuesday! I've got 11 agar plates active right now, 3 clones from my malabar grow, 3 transfers from melmac germ plates (thanks Qirk!), and 6 germ plates with spores from Roar!

The melmac transfers:



The malabar clones (rather bacterial I know, but one I can transfer away and the other looks clean so it's a success in my book!):



All the plates together:






Those lids says PET1. I couldn't of sterilized them so have do you go about this?:takingnotes:



Tbh I just soaked the lids in iso and hoped for the best. None of them ended up contammed so it worked out. The cups themselves are pp5 and I properly PCed them.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycanthrope]
    #27200949 -

Lol at the transfer to empty dish. I've opened empty ones in a stack ready to transfer but stopped before actually putting anything on them.

I saw a post someone made about pouring 40 plates and then realising they didn't have agar agar in :smile:


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p9hu7's clean spawn thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: clockworkshroom]
    #27200964 -

I mixed 1g activated charcoal in 500ml water for last nights run, it looked an ok colour in the media bottle but produced a really shitty, messy, light grey. Should have been 1g per 100ml. I will not be produced D3monic looking plates just yet :smile:


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p9hu7's clean spawn thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: clockworkshroom]
    #27201028 -

Yea ... I 1g per 100ml but honestly I don’t measure. I just do like half a spoon scoop full 🤣 but peeps wanted exact measurements so I weighed a scoop and went with the average for the tek. I’m never that “exact” with it personally.

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Shop: North Spore Injection Grain Bag   Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds


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