BOD's DIY Inoculation Loop TEK - Mushroom Cultivation

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bodhisatta
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Registered: 04/30/13
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BOD's DIY Inoculation Loop TEK

9
#24292526 - 05/03/17 03:47 PM (4 years, 9 days ago)

This tek is part of bod's cultivation is easy as fuck link list

Bod's DIY Inoculation Loop TEK
Using X-Acto style handles and nichrome wire or easily found as vape coil wire

You Will Need

Some wire, experiment with different lengths 3.5-4 inches works well for me.
some sort of thin rod to make the loop with, I used a thermometer
and a handle, I used the x-acto for the clamping mechanism.

This is how we do
Take the x-acto knife apart

fold a wire over to make a U shape and put the pointy ends into the blade receiver of the x-acto handle

now you have one of these

put your thin rod thing through, then push the wire so that it forms a loop around the utensil before you start to twist

if you don't do that

ugly mess

if you spend the time to smooth it out

I also buy sterile disposable ones with much smaller loops 1 microliter(uL)

they will both work for streaking spores out. With these ones you can bend your loop's eye to make it smaller to hold onto a drop of solution.
for example take this

then crimp it into

this

Flame away

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hamloaf
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Re: BOD's DIY Inoculation Loop TEK

[Re: bodhisatta]
#24292533 - 05/03/17 03:49 PM (4 years, 9 days ago)

Nice one.

--------------------

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Organic_Magic
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Re: BOD's DIY Inoculation Loop TEK

[Re: bodhisatta]
#24292541 - 05/03/17 03:51 PM (4 years, 9 days ago)

This is EXACTLY how I do it. People always forget the versatility of an x-acto handle.

Good stuff bod. :thumbup:

--------------------
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mushboy
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Re: BOD's DIY Inoculation Loop TEK

[Re: hamloaf]
#24292545 - 05/03/17 03:53 PM (4 years, 9 days ago)

tight

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mynakedrat
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Registered: 02/16/17
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Re: BOD's DIY Inoculation Loop TEK

[Re: mushboy]

1
#24292551 - 05/03/17 03:55 PM (4 years, 9 days ago)

I do this too!
I use a bread tie, remove the paper /plastic edges.
Awesome once again sir! :thumbsup:

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SuperFly
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Re: BOD's DIY Inoculation Loop TEK

[Re: mynakedrat]
#24292579 - 05/03/17 04:05 PM (4 years, 9 days ago)

Quote:

mynakedrat said:
I do this too!
I use a bread tie, remove the paper /plastic edges.
Awesome once again sir!

I do the same thing lol. I was thinking this looks so much nicer than my garbage twisty-tie.

Bod is swiping two prints at one time... Now that's gangster :laugh2:

Edited by SuperFly (05/03/17 04:18 PM)

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mynakedrat
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Re: BOD's DIY Inoculation Loop TEK

[Re: SuperFly]
#24292583 - 05/03/17 04:07 PM (4 years, 9 days ago)

Can contams get in the twists in the loop though?
Do you always flame the entire thing?

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hamloaf
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Re: BOD's DIY Inoculation Loop TEK

[Re: mynakedrat]
#24292589 - 05/03/17 04:09 PM (4 years, 9 days ago)

I always flame the entire wire.

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bodhisatta
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Re: BOD's DIY Inoculation Loop TEK

[Re: mynakedrat]
#24292595 - 05/03/17 04:14 PM (4 years, 9 days ago)

Quote:

mynakedrat said:
Can contams get in the twists in the loop though?
Do you always flame the entire thing?

in my agar tek I talk about how I keep a jar of 91% iso like a baby food jar. I dip my tools in there first and then flame them red hot. this way flame gets into all the crevices on tools.
This is how a glass spreading rod would be sterilized since you can't hold glass in a flame till it's red hot and then touch a petri dish, it won't cool down fast enough. And it will eventually shatter too.

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Munchauzen
Mushroom Maharaja

Registered: 06/23/11
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Re: BOD's DIY Inoculation Loop TEK

[Re: bodhisatta]
#24292742 - 05/03/17 05:25 PM (4 years, 9 days ago)

Im sure you meant to say 70%, not 91%.

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bodhisatta
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Re: BOD's DIY Inoculation Loop TEK

[Re: Munchauzen]
#24292746 - 05/03/17 05:26 PM (4 years, 9 days ago)

Nope 91 is more flammable burns hotter. And i would imagine has lower surface tension to get in more places

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Munchauzen
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Re: BOD's DIY Inoculation Loop TEK

[Re: bodhisatta]

2
#24293018 - 05/03/17 07:32 PM (4 years, 9 days ago)

Quote:

bodhisatta said:
Nope 91 is more flammable burns hotter. And i would imagine has lower surface tension to get in more places

butane burns at 3578°F. 91% burns at 750°F.

70% is proven the more effective sterilizer. and the cracks and crevices get red hot from your butane torch.

so I guess im just not seeing the reasoning here. are you not using butane? if not, why not?

Edited by Munchauzen (05/03/17 07:55 PM)

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bodhisatta
Smurf real estate agent

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Re: BOD's DIY Inoculation Loop TEK

[Re: Munchauzen]
#24293145 - 05/03/17 08:40 PM (4 years, 9 days ago)

Propane torch anyway. 91% is just always what I've seen used for flaming glass rods. You're not using the alcohol to sanitize

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d0urd3n
Just call me "D"

Registered: 09/15/10
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Re: BOD's DIY Inoculation Loop TEK

[Re: Munchauzen]
#24293149 - 05/03/17 08:41 PM (4 years, 9 days ago)

Really simple, but great. I have an exacto sitting around since I switched to an actual scalpel. Will be trying this even though regular wire worked just fine. This looks a bit more pro/easier to grab with gloves on.

:amusedapplause:

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Kenetic
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Re: BOD's DIY Inoculation Loop TEK

[Re: d0urd3n]
#24293163 - 05/03/17 08:49 PM (4 years, 9 days ago)

Damn this is an excellent tek! Too bad i already have one lol. Thats creative though and looks nice.

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markymark777
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Re: BOD's DIY Inoculation Loop TEK

[Re: bodhisatta]
#24461606 - 07/06/17 12:09 PM (3 years, 10 months ago)

I am very inexperienced here, and maybe this is a stupid question, but what is the reason you wouldnt just use the syringe to squirt a drop on the petri dish. Is it just to get the spores to more areas on the dish, or am i missing the point entirely?

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bodhisatta
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Re: BOD's DIY Inoculation Loop TEK

[Re: markymark777]
#24461613 - 07/06/17 12:13 PM (3 years, 10 months ago)

That is the reason.
You don't want spores all sloppy over a dish
And the more spore solution you use the higher your chance of contamination.
And too much spore solution makes all the growth happen irregularly all over the dish so it's harder to grab glean mycelium.

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mushpunx
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Re: BOD's DIY Inoculation Loop TEK

[Re: bodhisatta]
#24461633 - 07/06/17 12:20 PM (3 years, 10 months ago)

Nice write up!

This is exactly the same as the loops they sell on lots of cultivation sites (I think these come from China).
Its just a handle and it comes with a bunch of nichrome wires

You can save yourself some $ doing it yourself

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3B3B4E
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Re: BOD's DIY Inoculation Loop TEK

[Re: mushpunx]
#24461981 - 07/06/17 02:59 PM (3 years, 10 months ago)

Cool tek Bod :thumbup:

This is the exact same thing I did, it works perfectly
I didn't use braided wire though, it's there a reason for choosing the braided?

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bodhisatta
Smurf real estate agent

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Posts: 60,910
Loc: Milky way

Re: BOD's DIY Inoculation Loop TEK

[Re: 3B3B4E]
#24461988 - 07/06/17 03:01 PM (3 years, 10 months ago)

use what you have

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