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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: J. Jack Flash]
    #25726982 -

The room wouldn t have to be the same temp as the agar.  The gradient is on a curve.  See β€œdew point”.  I can’t quite remember the data from biology courses, but it’s not as tight as you think it is. 

Frankly, I ain’t gonna fuck with it.  I might try bods fix at some point but as long as the droplets stay out on the lid, I don’t care much.


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Need a lift?

Edited by BeefExtreme (01/06/19 11:17 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 1
    #25727010 -

yes it would. it's why stacking works. the lid is heated to the same temp as the agar, so the vapor, which is also the same temp as the agar can't transfer heat to it and condense. it's not biology, but thermodynamics.

edit: and i agree the excess moisture isn't a problem of its own. storing plates upside down helps too.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens]
    #25727042 -

Duhrwood Stevens said:
Not too much water, too hot when poured


Exactly this... condensation is caused by a temperature difference on either side of a given barrier (petri lids in this case) so the stacked plates have heat above and below the lid, which reduces/eliminates condensation... the larger the temperature difference  (cooler the room), the more condensation that occurs... if your technique was sterile, it won't hurt anything, just unsightly and more difficult to view... I mix agar with as little as 3g agar powder to 500ml of water, it's not too much water in the mix... turn the heat up when you pour for about 30 minutes after: I run it about 80, and pour when I can comfortably hold the vessel for a  long period... about the temperature of coffee you can drink quickly... sometimes it's starting to gel up before I get the last few poured; that's when I know most of my plates will be dry


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25727772 -

These guys took off quick..just did some transfers to agar and lc. About to put it on wbs.



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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #25727776 -

I let mine go longer than that.


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Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #25728009 -



I know I got the funk but what about the 3 white spots?


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Spore Collection
Thai Lapi Yai 2018 end of year grow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25728034 -

Hard to say from the photo... they're small too... I typically wait until the growth is dime sized to determine if it's mycelium


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25728046 -

Caps I only got a few funky plates out of 8. Hopefully I'll see some growth soon. This is my pictures from my current thread.




Praying to the mycelium  gods


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Spore Collection
Thai Lapi Yai 2018 end of year grow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25728303 -

For the last many years I have only used spore prints to agar.
I acquired a few spore syringes and want to inoculate to agar. I attempted one of them and both agar dishes were contaminated.  Any good procedures to get those syringes going good on agar.

- Used SAB
- Flame sterilized needle point
- Pushed out a bunch of drops before going to agar
- dropped approx 5 drops to agar
- Flame sterilized a paper clip to use as an inoculation loop to smear around.


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Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhosCap]
    #25728368 -

Shake the syringe well before flaming... Only use one drop to cool... Only use one drop to inoculate... be sure to hold your syringe nearly horizontal to avoid hovering over the dish, and drop one drop in the center of the dish without touching the agar with the needle... dont worry about spreading it and transfer once the mycelium is dime sized or outgrows any obvious contamination


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25728404 -

Thanks McGee.
Will do that on next agar operation :awesome:


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Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhosCap]
    #25728687 -

I sometimes  get a contam or two on in germ plates,  not always.  Just transfer the best, leave the rest.


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Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25728696 -

Fungeyes said:
Caps I only got a few funky plates out of 8. Hopefully I'll see some growth soon. This is my pictures from my current thread.




Praying to the mycelium  gods


Hard to tell from the angle of the pics, but I don't see any myc in there.


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It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25728715 -

All things agar!  So far, so good.  As noted in previous posts I poured and added tissue samples for the first time, fingers crossed on those plates as the fruits were meaty.  I picked up some great anti-condensation tips for the future and all is well in the world.  Now for some clarification.... 
I believe it was Bod that pointed out the massive amount of transfers from a MS inoculation of agar for strain isolation and recommended just fruiting from MS and then cloning the best fruits.  I have ordered my last grip of different MS syringes from my favorite vendor and they should arrive this week.  I am ultra comfortable with the PF tek and I'm under the impression that using the MS syringes for the PF tek and then picking the best fruits to clone would be the quickest and simplest way to continue the best genetics of the differing strains with fewer plate transfers.  Anybody want to chime in their own two pennies?  I'm cool with doing the fruit to sample to agar but I'm down to try out MS to agar as well.  Shout if you have better ideas.  I have time and a great workspace, plus plenty of millet WBS mixes too, so if MS to grain to fruit is a better method I'm down like a clown for that project as well. Side note, I have read through the entire agar tek both for clone transfers and MS plate streaking. Just fishing for other opinions on best route for these MS syringes.
Thanks for previous help and look forward to continuing thoughts.


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It looks a lot more like it does now than it did...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
    #25728870 -

I think it was my suggestion, but a little elaboration:
Isolating from spores (without lab equipment) is a painstakingly long process,and could potentially yield a strain that is weak, low yielding, or won't fruit at all... by cloning a cluster fruit, you ensure a base culture to work from that in fact fruits, and in clusters... potency is still undetermined until bioassay, which is why I like to pick 5 clones from MS, grow them out side by side in shoeboxes to determine a winning culture based on colonization speed, yield, potency, and  phenotype... I've found that original clone cultures outperform 11 of 12 isolates derived from it... don't necessarily need a true isolate, just a well performing monoculture:  which most clones will provide


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25729105 -

Sorry Caps for the potential misquote!  I read so much the names tend to blur a bit.  Sounds straight up to me and definitely how I am leaning.  I'll put together some jars and get em going so I have some pretty cluster specimens to choose from.  I keep a very detailed notebook journal of all of my info, so this will be pretty easy to track all labeled out.  Thanks for the reply!
HH


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It looks a lot more like it does now than it did...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
    #25729120 -



Gettin ready to throw this in the PC



Hey Oak Ridge, cant wait to see how she does.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RandyFeller]
    #25729122 -

Holy fucking rhizo Batman!


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25729134 -

The most rhizo growth I've seen so far outta Hualta, PE, PEU, RW, Cambo, and Tidal Wave.

Hope its a sign of good things to come. Feel like I need to get more non fussy varieties in the mix as I have a shit ton of PE going. Growing something faster than PE will help soothe my A.D.D. haha

Sure does look cool af, like strands of dental floss or something.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RandyFeller]
    #25729149 -

I'll post this here since it seems I've beat my battle with reoccurring condensation on petri cultures and can hopefully help others. Lots of good input from others and an eventual fix.


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When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps

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