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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Cabin Sequestering
    #29086059 -

Looking for better understanding of how to do this properly. I know to cut a square in the center, put contaminated piece in center and cover with a sterile piece of agar like a roof but I’m lacking knowledge of details. Like should the receiving bottom square be clean all the way to the dish? Should contaminated wedge be in center without  touching the walls? Etc. please help 🙏🏼

Edited by Ill-bird (01/12/25 09:56 AM)

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Re: Cabin Sequestering [Re: Ill-bird]
    #29086798 -

you'll have quicker response Here

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Re: Cabin Sequestering [Re: Ill-bird]
    #29086961 -

Hello!

I've been using this technique for (? some small time - weeks ?) & I've used it as my starting point for several experiments. I cut my cabins clean through the agar to the dish & keep them quite small, maybe the size of a pea. I inoculate the cabin using an inoculation loop, but you could do the same with any tool.

Here's the original article with full details - it's a quick read, can download PDF from there.

Shi XX, Qiu HP, Wang Jy, Zhang Z, Wang YL, et al. (2019) A handy method to remove bacterial contamination from fungal cultures. PLOS ONE 14(11): e0224635. https://doi.org/10.1371/journal.pone.0224635

Take care - hope to see ya around!


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Re: Cabin Sequestering [Re: LewDoja]
    #29090012 -

I appreciate the replies

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Re: Cabin Sequestering [Re: Ill-bird]
    #29106145 -

In this post are the following nuggets:
Spores and Cultures: A Comprehensive Guide

Quote:
SupaThaRipper said:

Cabin Sequestering Text

Cabin Sequestering Video




The demonstration in the video starts at 23:28 he kinda talks a lot lol but it seems like a conference talk or something so that's to be expected.

I don't know why this took me so long to find, I feel like I had seen people talking about mentioning Cabin Sequestering (CS) in the agar thread but I hadn't really come across it in any of the comprehensive agar guides or teks.

It seems like its a really good way to get rid of bacteria it seems like it should be standard practice after a germ plate.

I'm surprised no one mentioned it to me when they said my spawn looked bacterial. Is there a reason folks don't like this technique? or have I just had my head under a rock for the few months I've been on here and this is common knowledge?

edit: a little write-up about how the paper describes CS working - and another useful post about sequestering

Edited by sage_bd (03/05/25 12:44 PM)

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Re: Cabin Sequestering [Re: sage_bd] * 1
    #29106245 -

It’s only really become common knowledge on the shroomery recently


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Re: Cabin Sequestering [Re: sage_bd]
    #29109799 -

It's just a variation or amalgamation of trenching and hot pour.

The latter I've been using since I started with success. Also helped my comeback after 10yr hiatus... on a matured invitro (plin) half pint ag-jar

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Re: Cabin Sequestering [Re: sage_bd]
    #29113147 -

Thanks for the insight everyone, I had heard about this method from someone, and while I got the jist of it, there were some misunderstandings as they were pretty vague about it.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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