3.A.M said:

realised that the bacteria’s spread is highly dependent on gravity

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Can you elaborate on that? when I searched for "effect of gravity on bacteria growth" I only get studies related to space and really high/low gravity/acceleration.

Here is a photo of some bacteria on spores:



I don't think gravity is a relevant factor in how CS works. I also searched the publication for the term gravity it doesn't show up.

But in the abstract it says this:

Quote:

A handy method to remove bacterial contamination from fungal cultures said:
we developed a new method for bacterial removal from fungi cultured on solid medium, the Cabin-Sequestering (CS) method, based on the different culture characteristics between fungi and bacteria. [...] After 7–10 days of culturing, fungal hyphae grow out along the edge of the coverslip; however, the contaminating bacteria cannot pass through the space formed between the medium and the coverslip and, thus, remain in the cabin. The newly grown fungal hyphae around the coverslip are re-inoculated into fresh culture plates, where they form bacteria-free fungal colonies.

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Quote:

paper said:
The M. oryzae hyphae can penetrate into the surrounding medium and bypass the coverslip to grow and form new colonies around it. In contrast, the bacteria are restricted by the solid medium and the coverslip, and, thus, they are retained in the cabins under the coverslip. The fungal hyphae around the coverslip are reinoculated onto new CM plates.

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el boso said:

Is there a way to separate the fungi i like from molds and other contaminants besides bacteria.

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kirkeng said:

Cabin sequestering has me fully converted, currently running pretty much everything I have through it after seeing preliminary result with nasty pan cyan Bangalore post sequester.

Before:


After:


Will update later but also testing it for cloning, small mushrooms are always a pain to clone. I’ve taken 4 samples of whole stipe sections from zapotecorum and placed entire sections in the cabin. Samples of clone tissue were handled with bare hands and cut with non sanitized scissors eg handled in a very dirty way without regard. They were placed within the cabins with sterilized tweezers however, just trying to convey I took no care to ensure tissue samples were clean in any regard, rather tried to make them dirtier. All samples are now starting to show initial faint threads emerging out the cabin, no bacteria can be seen yet. Will update with pictures once things are more visible for pictures.

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sage_bd said:

3.A.M said:

realised that the bacteria’s spread is highly dependent on gravity

---

Can you elaborate on that? when I searched for "effect of gravity on bacteria growth" I only get studies related to space and really high/low gravity/acceleration.

Here is a photo of some bacteria on spores:



I don't think gravity is a relevant factor in how CS works. I also searched the publication for the term gravity it doesn't show up.

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Sorry, only just saw this and thought it best to clarify, in all other cases CS worked in halting bacteria except for whatever was growing on these wild fruits, I’d find the bacteria escaping the cabin at the same time as the myc and growing in thick viscous blobs extremely fast filling a plate within days, by angling the plate the growth and spread of bacteria upwards was slowed by gravity giving the myc a better advantage to grow through beforehand.
While many bacteria can move in their own limited way their most common form of growth is simply fission, exponential growth over a food source, that growth is not unlike water spreading over a surface from a central point and gravity will always dictate movement to certain areas quicker than others, I simply used that to my advantage