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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Spore Bacterial Infection?
    #1557782 - 05/18/03 02:54 PM (18 years, 5 months ago)

Afriend of mine made up a batch of syringes, 7 to be exact, and shared 3 of them with a girl, My friend innoculated 12 jars with one syringe and so far 5 have survived she on the other had used 3 syringes for 6 jars, and all of hers have contamned or stalled, anyways she says the spores have a bacterial infection which ive never heard of, and that even though the surviving jars look healthy they are contamned. IVe never heard of a bacterial infection, these spores where made from a spore print and my friend tried to spread out the print as much as possible (making 7 syringes) as to avoid contamns. Personally I think the fact that she used 3 syringes for 6 jars is why none of her jars survived, and my friend using only 1 syringe for 12 is why 5 of my friends survived. Anyways has anyone heard of a spore bacterial infection or is she just blowing it out her ass?


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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/21/03 10:50 AM)


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Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1557792 - 05/18/03 03:04 PM (18 years, 5 months ago)

There may be bacteria in the print, which causes an infection after inoculation. How long did it take for the jars to go bad? Did they all go bad with the same thing?

Yes -- the more liquid you use the more chance of contamination unless you are sure it's 100% sterile.

--
Micro


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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: micro]
    #1557797 - 05/18/03 03:07 PM (18 years, 5 months ago)



--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/20/03 12:49 AM)


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OfflineMykeOfile
stranger thanmost
Registered: 04/30/03
Posts: 212
Loc: behind the bookshelf
Last seen: 17 years, 8 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1557811 - 05/18/03 03:16 PM (18 years, 5 months ago)

yo, uve already expressed u respect for my opion and all, but theres this book u might of heard of called The Mushroom Cutivator, and i believe the authors a pretty excepted expert, and says that the spores themselves can be a source of contamination, btw, not everybodies has been on this board as long as theyve had this hobby, no need to be an ass and gett all high and mighty(but lets not blow up the thread, if that pisses u off, u can pm me)


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u know it aint funny when u aint got no money and the clock strikes 420 and u wanna get high
~Kottonmouth Kings


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: MykeOfile]
    #1557859 - 05/18/03 03:55 PM (18 years, 5 months ago)



--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/20/03 12:50 AM)


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Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1557869 - 05/18/03 04:02 PM (18 years, 5 months ago)

Understandable, but I'm no newbie. Just haven't been on the Shroomery for that long.

I wouldn't make anything up, however. There is such thing as peer review.

Next time if you only want people to answer who have been here for a while you should put it in your post so I don't waste my time.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineRustik
Where am I?

Registered: 04/18/03
Posts: 289
Last seen: 6 years, 11 months
Re: Spore Bacterial infection? [Re: micro]
    #1557933 - 05/18/03 04:41 PM (18 years, 5 months ago)

Sounds like the syringes were contaminated.

Bacterial contamination results in a foul odor (do NOT take a whiff of your jars, if you must smell them then slightly open the lid a 'waft' some air from the lid in your direction.) If it smells like rotten apples/smelly feet, it's probably bacterial. Bacterial contaminations aren't always obvious, but you may notices slowed/stalled growth followed by a milky tan colored liquid forming in the bottoms of your jars.

Quote:

yo, uve already expressed u respect for my opion and all, but theres this book u might of heard of called The Mushroom Cutivator, and i believe the authors a pretty excepted expert, and says that the spores themselves can be a source of contamination, btw, not everybodies has been on this board as long as theyve had this hobby, no need to be an ass and gett all high and mighty(but lets not blow up the thread, if that pisses u off, u can pm me)




TMC is a very good book, and yes, the writer and contributors are very well respected individuals. However, I think you may have misconstrued the information. Spores themselves can NOT be a source of infection (so long as they are completely isolated). Spore syringes can contain contaminants (including molds/yeast/competitive fungi/bacteria), as can spore prints. P. cubensis spores cannot contaminate themselves.

The exact answer to your question is this: The spores, which are very tiny, cannot be infected by a bacterium themselves. There may have been bacteria in the solution they were in (inside the syringe), but you cannot infect a spore with bacteria.


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The blue... the blue!!!


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: micro]
    #1557945 - 05/18/03 04:45 PM (18 years, 5 months ago)



--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/20/03 12:50 AM)


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1557989 - 05/18/03 05:04 PM (18 years, 5 months ago)

There are several variables where a clean syringe can GO WRONG.

First, the syringe may carry a contam.

A contam can be introduced during innoc procedures.

Content of jar may not be sterile, leading to contam's germinating in it.

To much spore solution in any jar can overload the moisture content of the jar, leading to a bacteria like contam.

The list goes on & on.......what may be wrong.

6T  :wink:


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineMykeOfile
stranger thanmost
Registered: 04/30/03
Posts: 212
Loc: behind the bookshelf
Last seen: 17 years, 8 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1557995 - 05/18/03 05:07 PM (18 years, 5 months ago)

to quote from pg 23 of TMC, "A spore print carelessly taken or stored can easily become contaminated" and if u look on pg 28 there is a pic of P.Cubensis spores that are infected with rod shaped bacteria, how can one misconstrue that?!?....(and TMC is the bible, i was being sarcastic)


--------------------
u know it aint funny when u aint got no money and the clock strikes 420 and u wanna get high
~Kottonmouth Kings


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: MykeOfile]
    #1558019 - 05/18/03 05:18 PM (18 years, 5 months ago)

You said, and I qoute "spores THEMSELVES can be a source of contamination", Your statement seems to suggest that cubenis spores themselves can be a source for contamination, plainly put that is a miscontrued statement. There is a difference between a spore print which has become contaminated, and spores themselves being a source for contamination. You proved it yourself with your qoute.


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/18/03 05:23 PM)


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OfflineMykeOfile
stranger thanmost
Registered: 04/30/03
Posts: 212
Loc: behind the bookshelf
Last seen: 17 years, 8 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1558041 - 05/18/03 05:27 PM (18 years, 5 months ago)

ok, now we're gettin picky, i did not say that the spores were the contamination, but the source, as in where it came from, obviously a mushroom spore is not going to itself grow into bacteria or mold or ne thing other than mushroom fungus, but if the spores are infected with bacteria(as in figure 29 on pg28 of TMC), then that is the source of ur contamination,


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u know it aint funny when u aint got no money and the clock strikes 420 and u wanna get high
~Kottonmouth Kings


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: MykeOfile]
    #1558048 - 05/18/03 05:31 PM (18 years, 5 months ago)

but that was not the question my question was Can spores (meaning spores themselves, not spore prints, this doesnt need to be clarified because if I meant spore prints i would have said spore prints) get a bacterial infection?, How is this picky, that was the question just because you chose to answer a different question doesnt mean im getting picky...Spores cannot be the source for contamination once again spores & spore prints are two different terms, you seem to think they are one in the same. As Rustik said spores themselves cannot become infected by bacterium,THAT was the question, and he answered it.And you did say spores themselves can be a source for contamination, just scroll up.


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/18/03 05:36 PM)


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OfflineMykeOfile
stranger thanmost
Registered: 04/30/03
Posts: 212
Loc: behind the bookshelf
Last seen: 17 years, 8 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1558064 - 05/18/03 05:40 PM (18 years, 5 months ago)

OMG, dog a spore print is nothing but the spore that have fallen out of a mushroom, if it gets infected then guess what , u have infected spore, and yes i did say the spores can be ur source of contamination, if 6T give u some info, 6T is not ur info he is ur source for that in for, if u touch ur substrate with a dirty fork then the dirty fork is not ur contamination, it is ur source of contams, if u inject infected spores into ur jars then the spores themselves are the source(WHERE IT CAME FROM) of ur contams, u just like tellin folks theyre wrong dont u


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u know it aint funny when u aint got no money and the clock strikes 420 and u wanna get high
~Kottonmouth Kings


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: MykeOfile]
    #1558085 - 05/18/03 05:50 PM (18 years, 5 months ago)

and im not talking about spore prints im talking about spores, not the collective spores which make up a print, but the spores themselves on an individual level, you are just missing the point completly my question was can spores themselves become infected with bacteria, how simple is that, I know unwanted spores can find their way onto spore prints, but that was not the question, My question was can spores get a bacterial infection meaning the spores on an individual level become contaminated, that was my question. Its not hard to comprehend a questioin for its wording. If spores could become infected with a bacteria, then the spores themselves could be the source of a contamination, but since they cant the only source for the contamination is unwanted spores. If I inject spores into a jar, the spores are not my source for contamination, the invader spores are the source of the contamination the mushroom spores dont affect it. Because the contamination doesnt come from the spores it comes from the invader spores.

if spore prints give u some spores, then spore prints are not ur spores they are ur source for those spores if spore prints become infected then the print has invader spores on it, that does not mean the spores are infected.



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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/18/03 05:57 PM)


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OfflineWahoo
Cap'n
Registered: 06/15/99
Posts: 32
Loc: right behind you
Last seen: 10 years, 7 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1558098 - 05/18/03 05:56 PM (18 years, 5 months ago)

For probably the 5th time in this thread: YES, spores can become infected by bacteria. Allow me to quote from the book of Stamets, chapter 2
Quote:

The greatest danger of doing concentrated multispore germinations is the increased possibility of contamination, especially from bacteria. Some bacteria parasitize the cell walls of the mycelium, while others stimulate spore germination only to be carried upon and to slowly digest the resulting mycelia. Hence, some strains are inherently unhealthy and tend to be associated with high percentage of contamination. These infected spores, increase the likelihood of disease spreading to neighboring spores when germination is attempted in such high numbers. (TMC, page 28)


He goes on to say that some fungi actually require another bacteria to be introduced into a culture before it will germinate.

Seriously, its a great resource book, no matter what your skill level.
ISBN: 0-9610798-0-0


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OfflineMykeOfile
stranger thanmost
Registered: 04/30/03
Posts: 212
Loc: behind the bookshelf
Last seen: 17 years, 8 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1558105 - 05/18/03 06:02 PM (18 years, 5 months ago)

my answer to ur question is yes, the spores can get a bacterial infection on an individual level, according to THe Mushroom Cultivator,i think u do not seem to understand my answer, the pic i keep refering to shows a microscopic veiw of individual spores with rod shaped bacteria growing on the spores themselves,.........u can argue symantics with me all day but i phrased it that way b/c thats what the book says: "There are five primary SOURCES of contamination in mushroom culture work:
1)the external environment
2)the culture medium
3)THe culturing equipment
4)THe cultivator and his or her clothes
5)THe mushroom spores or the mycelium"


--------------------
u know it aint funny when u aint got no money and the clock strikes 420 and u wanna get high
~Kottonmouth Kings


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: MykeOfile]
    #1558127 - 05/18/03 06:15 PM (18 years, 5 months ago)

If thats what you meant then thats what you should have said "A spore print carelessly taken or stored can easily become contaminated" That was your answer, but it was too the wrong question, you could argue semantics, or we could argue about your spelling, but its unnecessary since you dont seem to be able to make a very clear point. Also the picture doesnt seem to show spores being consumed by bacterium more like spores with bacterium.


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


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InvisibleArmFromTheAbyss
Old Hand

Registered: 10/09/02
Posts: 1,368
Loc: Down here in Babylon
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1558128 - 05/18/03 06:16 PM (18 years, 5 months ago)

Aight dude first off, there's no need for unnecessary bickering. Positive vibrations.

Second, I think the girl you mention in the original post was wrong to narrow it down to the spore print as the contam source. As 6t said, it could be a number of vectors of contamination. She couldn't know too much if she used 3 syringes for 6 jars. Thats excessive. Most likely, she was the source of contamination since your jars were fine. She meaning her doing all the procedures carelessly.


My question was can spores get a bacterial infection meaning the spores on an individual level become contaminated, that was my question.

It is not that likely for the actual spores to get infected by bacteria. Sure, there could be bacteria mixed in with the spores. But bacteria need h20 to germinate. I doubt the spores on the spore print would have any sort of bacterial germination. Inside the spore solution yes, maybe.

How long were these syringes sitting around?

Btw, it doesn't really matter how long you've been around here, it matters how much experience you have in cultivation.


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: ArmFromTheAbyss]
    #1558134 - 05/18/03 06:22 PM (18 years, 5 months ago)

My friend used his spores almost immedietly after making them from a print and allowing the jars too cool around the same times. she picked up her syringes a few days later.  Some of his jars survived.  As I said earlier I dont have a problem with new members especially the experienced ones who offer up their invaluable knowledge, but it seem most of the bs, invalid info comes from newer members who just read the FAQ, and are raring to give advice. anyways ive gotten off-topic.  Thanks for all the answers  :wink: 


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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/19/03 09:38 AM)


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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1558194 - 05/18/03 07:02 PM (18 years, 5 months ago)

I hope nobody ever gives you anymore advice or bothers to
answer your posts.

1. the question is positively retarded, EVERYTHING is a potential
source of contamination. ESPECIALLY carelessly prepared prints
and syringes.

2. insulting or otherwise belittling people who have attempted to
address your question over things as superfluous and generally
irrelevant as registration date is idiotic.

look at your reg date...2000...and you asked a question as remedial
as this? based on your logic, you should be an expert by now, right?

the very first reply not only answered the question but also sought
more information in an attempt to help you narrow things down.

may a wave of contamination infect your crop, ingrate.


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Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 142
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1559410 - 05/19/03 05:53 AM (18 years, 5 months ago)

You have been getting some good and clear answers to your questions and being a total ass about it.
And yes, spores can become infected. Bacteria can breach the cell walls and migrate inside to munch on the goodies. Spores rot like everything else organic but the bacteria must first come from a source outside the spores. This outside source could be the print itself or the spore suspension or any number of sources. If spores are contaminated with bacteria on the inside you can bet they are also contaminated on the outside. When your girl friend said she thought the "spores" were contaminated with bacteria she was obviously not referring to the intracellular integrity of the spores themselves but to their collective environment both internal and/or external. Spore prints, spore syringes, and even spores themselves can become contaminated with bacteria, yeasts, molds, and even viruses. Whether the contam is intracellular or extracellular doesn't make a hoot of difference. The result is the same...contam, contam, contam.


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InvisibleBlueDemon
stranger thanever
Registered: 05/19/03
Posts: 4
Loc: Gate of Hell
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1561767 - 05/20/03 12:06 AM (18 years, 5 months ago)

Dear Squirrely Blade,

I am not new to this hobby by any means, but I am a new member, so no doubt you would demean any advice I give you. In this thread, and many others where you posted, I noticed that you do not show very much respect to your fellow Shroomerites (unless you are kissing up to a senior member), and many of them have remarked, even in this thread, how assholic you are when they try to help you. Since this is your thread, I don't feel I must restrain myself in providing a mirror for you to look at yourself. Your motto in your sig, shown below, is perfect. You are completely subjective to the point of being squirrely, IMO, so YOU ARE the "Squirrel Master". Please read a history of your own posts below, nothing taken out of context, just a nice little history of you, for you to think about the next time you decide to crap on somebody as a Newbie, Mr. Squirrel. Make up your mind...are YOU a newbie too, or do you really know what you're talking about FROM EXPERIENCE in all your posts quoted below, where YOU have provided advice ? Happy READING !!!!

YOUR SIG: "We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity"

....yeah, right.

APRIL 8, 2003, YOU ADMIT THAT YOU DON'T EVEN HAVE A PRESSURE COOKER: "No can do...I dont have a PC...I was thinking of boiling some poo & water together letting it cool then using the water in the jars before i boil the jars is this a bad idea?"

MAY 19 YOUR THANK-YOU FOR AN ANSWER (IN THIS THREAD) IS THIS, WHERE YOU TOTALLY "DIS" NEWBIES: "I was looking for someone whos been here for say more than 30 days to answer this question... I'd just rather have more experienced mycologist posters, give me my cultivation information, thats all...nothing about high and mightyness its about getting information from a reputable source You didnt even answer the question i asked, dont waste my time...I just think there is an over abundance of bs advice on these boards coming from people who havnt been here very long, dont know what their talking about, and answering questions which were not asked."

MAY 15, YOU ARE SUDDENLY A TOTAL NEWBIE JUST IN TIME FOR THE CONTEST: "Well am I newbie, I must say I've been around the community for quite some time, and on certain occasions which seem to be decades away I have been blessed with mushrooms. Unfortunatly all attempts minus my first two have been horrible failures. I'll start with my first grow after a 3 month waiting period begginning with the ending of my last successful grow. Using 1syringe of the
PF classic strain I innoculated 10 jars of BRF/Verm boil sterilized for 1 hour, well needless to say most of the jars stalled, and contamned and the others didnt germinate, and then contamned. Well needless to say this sucked, but i wasnt discouraged I quickly cleaned the jars sterilized the enviroments and set out again. then innoculating 8 pf jars with strophoria strain from pf, well again the jars failed, and contamned, So thats pretty much my experience in mycology"

MAY 13 YET, TWO DAYS EARLIER, YOU ARE AN EXPERT ON PRINTS: "Well it depends, If the print is contamned but the un-wanted contamn only occupies one section of the print, and you put it all into one syringe, your screwed, you literally just put all your eggs into one basket, and it turned out for the worse. on the other hand, if you are sterile, and it works out for you, and you have the prints to spare, why not"

MAY 13, your helpful answer to a question on coir: "Read the Tek..."

MAY 13, TWO DAYS EARLIER THAN THE CONTEST,YOU ARE AN EXPERT ON CLONES AND ISOLATES: "They are pretty similiar the partially attached veil is still noticeable, I would say the one kush posted are isolates, and clones, and the ones in my picture are the result of multi-spore innoculation..."

MAY 12: A POST BY ANOTHER SHROOMER WHERE HE ACTUALLY QUOTES A SHROOMERY FAQ: " Well... if your humidity gauge only shows 60, mabe you didn't put enough water in the perlite, in about an inch of perlite, at least 1/2 an inch should be water, make sure you maintain that 1/2 inch of water in 1 inch of perlite... just some info from the perlite FAQS around here."

MAY 12: YOUR ANSWER TO THE ABOVE POST (HIS QUOTE OF A FAQ), CRAPPING ON HIM: "1/2 inch of water is NOT neccessary in 1in of perlite, I know people who dont have any standing water, they soak the perlite in water, and then put that wet perlite in the bottom of their terrarium, This keeps the terrariums plenty humid, It doesnt matter how much water is in with the perlite aslong as the perlite is not fully submerged, and can wick the moisture into the air. so once again we have a NEWBIE GIVING BS ADVICE..."

MAY 12 A LITTLE CONFUSING ADVICE FROM YOU: "but remember these casing layers are non-nutritous so the smaller you get the less nutrients available for your fruits."

May 12: YOU ADVISE AGAINST GIVING ADVICE... "one question why are you giving direction to others especially information which can be found in the sticky..."

May 10:, TODAY, YOU ARE NOW AN EXPERT on POO, IN CONTRAST TO HOW YOU PRESENT YOUR FAILURES, ABOVE, FOR THE CONTEST: "Keeping jars sterile is an issue, but Colonized poo would not introduce any un-wanted organism's as long as sterile conditions are kept during the process, and especially if H2O2 is used. Also I gave two example's one other than the poo to poo, and that is using colonized poo in a casing."

MAY 10, YOU TOTALLY UNDERSTAND EXTRACTIONS: "As far as using 100% colonized jar to extract psilocybin, well it's highly recognized that mycellium has very little psilocybin prior to pinning, so eventhough everclear can be used to extract psilocybin, you will end up with none or very little, and that jar of crushed up BR well, most everything else just doesnt add up too well"

May 8: YOU ASK A REAL NEWBIE QUESTION: "afoaf sterilized jars and made spore syringes at the same time, he innoculated jars when they were cool about 10hrs later, did the spores have time to rehydrate, and can this slow the germination"

Apr 29: IN CONTRAST TO HOW YOU TREAT NEWBIES, YOU ARE ASS-KISSING NICE TO A SENIOR SHROOMERY MEMBER: "Good Lord man those are some AMAZING fruits my hat goes off to you Joshua."

Apr 3: YOU ASK ANOTHER REAL NEWBIE QUESTION: "when mixing the water from the syringe to the spores into a shot glass, how much water should i expell from the syring into the glass, the entire syringe worth isn't neccessary is it?, also how long should i wait after flame sterilzizing the blade for scraping the spores off the print, and flaming the syring needle?"

So, are you just another NEWBIE who SHOULDN'T BE GIVING ADVICE, like the ones you keep crapping on ?

Squirrel. Hypocrite.


Edited by BlueDemon (05/20/03 12:27 AM)


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Invisiblezero
???
 User Gallery
Registered: 11/02/99
Posts: 234
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1561949 - 05/20/03 01:14 AM (18 years, 5 months ago)

3 syringes for 6 jars is wayyy
too much moisture.
cause of stalling or bac. infection.
right??
seen it.
someone back me up.
peace,
zero


--------------------
sk8 or die


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InvisibleBlueDemon
stranger thanever
Registered: 05/19/03
Posts: 4
Loc: Gate of Hell
Re: Spore Bacterial infection? [Re: BlueDemon]
    #1562403 - 05/20/03 07:53 AM (18 years, 5 months ago)

Oh,

ALSO, for all NEWBIES reading this who have been crapped on by Mr. Squirrel, apparently when you disagree with him or critique him in any way (even though he thinks he can freely critique YOU) , he immediately goes in and RATES you with "one mushroom".....looks like that's what he did to me.

SO, just remember that YOU CAN RATE BLADELSD TOO! Just simply click on his name next to his avatar, and it will take you to a screen where you can rate him AS YOU BELIEVE HE DESERVES!. DON'T FORGET.....ONE is the LOWEST RATING !!!!



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OfflineDank420
Shrooms...Mmm

Registered: 04/12/03
Posts: 548
Loc: 'burbs of philly
Last seen: 13 years, 7 months
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1562415 - 05/20/03 08:23 AM (18 years, 5 months ago)

I never really liked BladeLSD, he's always assuming crap.


--------------------
"I tried marijuana once. I did not inhale." -William Clinton.


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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,145
Loc: my room
Last seen: 21 hours, 34 minutes
Re: Spore Bacterial infection? [Re: BlueDemon]
    #1562535 - 05/20/03 10:25 AM (18 years, 5 months ago)

5 shrooms for you.


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InvisibleBlueDemon
stranger thanever
Registered: 05/19/03
Posts: 4
Loc: Gate of Hell
Re: Spore Bacterial infection? [Re: Anno]
    #1563850 - 05/20/03 09:15 PM (18 years, 5 months ago)

Anno and Dank420......Thanks.

The Shroomery is a wonderful place...... for great sharing, learning, camaraderie, and fun.

If we all act with integrity and treat fellow Shroomerites - including "newbies" - with genuine respect, it can only get better and better.





Edited by BlueDemon (05/20/03 09:30 PM)


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OfflineGoodOlBattonAss
Stranger

Registered: 03/31/03
Posts: 333
Last seen: 17 years, 9 months
Re: Spore Bacterial infection? [Re: BlueDemon]
    #1563905 - 05/20/03 09:44 PM (18 years, 5 months ago)

Ha friggin ha..you did your homework didn't you?


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Invisibleafoaf
CEO DBK?
 User Gallery

Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
Re: Spore Bacterial infection? [Re: BlueDemon]
    #1563986 - 05/20/03 10:10 PM (18 years, 5 months ago)

phenomenal work!

/me wipes a tear from my eye.

I think you might just be DBK material...



--------------------
All I know is The Growery is a place where losers who get banned here go.


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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
Re: Spore Bacterial infection? [Re: BlueDemon]
    #1564016 - 05/20/03 10:21 PM (18 years, 5 months ago)

LOL! :grin:

thank you so much for doing that...

you would not believe the private messages this asshole
sent to me after I made my post above.

I tried to talk reason with him, but he continued to call me
a whore bag and that I should be gang raped and that I
was a fucking bitch.

funny enough, I pointed out a couple of those very same
posts to him, most notably the contest one and he flipped
his lid...

I strongly urge that people boycott this guy, don't reply
to his threads, don't trade with him, don't take his advice.
I know the shroomery well enough that this is not the
norm and that people like this need to be taught a lesson.

I'm a bitch, but I ain't a whorebag! LOL! :wink:

please feel free to send him a PM expressing your feelings
about his behavior! :smirk:


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InvisibleBlueDemon
stranger thanever
Registered: 05/19/03
Posts: 4
Loc: Gate of Hell
Re: Spore Bacterial infection? [Re: shroomQT]
    #1564326 - 05/21/03 12:02 AM (18 years, 5 months ago)

Everybody,

Thanks for speaking out !!!!!

and.... ShroomQT,

Sorry you had to put up with that PM crap from asshole BladeLSD. But thanks for standing up, stating your case, and helping to bring the truth out into the open. BladeLSD SHOULD be boycotted.....you simply don't treat other people that way, especially when they are trying to help you. And quite frankly, the kinds of things he was writing to you in the PMs sound a little psycho and sicko.

I noticed that a couple of "The Blade Squirrel's" posts, even in this thread, have already been edited and deleted by him......they are now blank. Maybe he intends to go back and try to find every instance where he shat on somebody and wipe out history. But enough people have spoken out now that everybody now knows the truth, and it won't matter..... but also,

HEY BLADE !!!! Don't bother. I downloaded enough of your crap to fill a BOOK while I was doing my "research", and if you abuse anyone else, I'll just post it again, so everyone can again see the "real you", and how poorly you regularly treat other people and misrepresent your level of experience and knowledge.

Again, folks, don't forget, you can RATE BladeLSD !!!!! Give him what he has earned !!!


Edited by BlueDemon (05/21/03 12:19 AM)


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 809
Last seen: 7 days, 17 hours
Re: Spore Bacterial infection? [Re: BlueDemon]
    #1565169 - 05/21/03 10:02 AM (18 years, 5 months ago)

I thought I would let it have a rest, but oviously you couldnt do the same, and there is no way I will let you make these statements which have been completly taken out of context, and miscontrued to your liking Blue. First you start with a yeah right of my sig, is this because you don't understand it ?, anyways thats not a big deal. So now to your exaggerations... ahh April 8th where I admit I dont have a pressure cooker, woohoo good for you found I dont have a pressure cooker, ok...I dont, never found it neccesary to move onto grains yet, Yes my thank you for an answer in this thread which did not answer my question, but did in fact answer a question which was not asked, but had you took the time to read further you would have discovered this. Also I entered a newbie contest, ok whats your point so did lots of other people who have been here for awhile. Now here is where you go for a long shot.

TWO DAYS EARLIER THAN THE CONTEST,YOU ARE AN EXPERT ON CLONES AND ISOLATES: "They are pretty similiar the partially attached veil is still noticeable, I would say the one kush posted are isolates, and clones, and the ones in my picture are the result of multi-spore innoculation..."

I claim to be an expert???? umm ya, sure if you think so...not...when talking about the malabar I said that one, was obviously due Multi spore innoculation, and the other to isolation and cloning, I devised this remark after talking to a friend who had grown malabar (he even sent me a print), and I think you will see I was backed up in that post smart guy.
Then there is the perlite post, ahh well I dont know what it says in the FAQ, and im sure not gonna take a gander cause you told me too, but It still stands 1/2in of water is not necessary in 1in of perlite, as long as the perlite is not fully submerged it is fine. so again we have an exaggeration by you.
Maybe the information was confusing for you, but if you took the time to see the question it answered you would notice its not so confusing. I answered a question asking How small can a casing be, and my answer was simple, casing is adding a non-nutrtious layer so the smaller you get the less nutrients available for fruiting, that may be confusing for you to understand, but If that member had a problem with it, I would have no problem clarifying for him in a pm, or subsequent post, I guess it wasnt a problem for that member Mr. exaggeration. Ohh so now to give information about something you are automatically an expert, well it seems like you are asking a bit more than even me, All I asked was they be here longer than 30 days by your own post, but Now by giving information that is claim to expertise, Well never have I claimed to be an expert, ever, so your just chock full of bullshit I have experience with colonized pasturized poo, thats why I gave up my advice because I have in fact used colonized pasturized poo, to innoculate jars, plain and simple I had experience with so I offered up my experience, that is all I do, and that is all I ask for... As for your final 3 exagerations, I needed helped so I asked a question. Joshua is an amazing mycologist, we had just become aquanted, and I found it fitting to congradulate his amazing fruits, so I did. So I think you will find I never claim to be an expert, but I do think I am qualified to give advice on certain topics which I do, and will continue to do regardless of what you think, and as for shroomqt, well I asked her many times to discontinue her pm messages to me, and only recently did they stop, She has said many things aswell, about me, about my mother, about me being dead, and frankly I think she got what she deserved since she harrassed me just as much as I to her, So have fun with your research blue, glad you have found a way to fill your days, With Crap, not to mention the fact that I stated in this post, and in others that I have no problem with new members at all, just new members who give bullshit advice, it sucks, and it has been cause for failure, but that isnt the point of this post, come to think of it neither is any of the second page to this post...


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


Edited by BladeLSD (05/21/03 10:10 AM)


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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1566146 - 05/21/03 03:49 PM (18 years, 5 months ago)

couterinsults were fired only after multiple
messages attempting to make things right
with you.

and I never wished you dead...now who's
exaggerating?

the fact that you edited all your posts is
enough of a concession of your guilt so
I won't hold my breath waiting for any
overt apologies from you regarding the
way you treated me and the other members
on the board.

best wishes and good luck with all your
future endeavors...


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OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,396
Last seen: 24 days, 22 hours
Re: Spore Bacterial infection? [Re: BladeLSD]
    #1566441 - 05/21/03 05:30 PM (18 years, 5 months ago)

This thread is getting out of hand and way off the original mark. The original question was answered [semantics aside].

Please go to PM for all things slander-related.


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