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Re: AGAR ENVY! (Anything and All things agar!) [Re: SwabMarley] * 1
    #28658649 -

Haha, hilarious.

Didn't know that one.

:bathtub40lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SwabMarley] * 2
    #28658800 -

SwabMarley said:
You ever seen Little Britain? It’s a little obscure, I’ll give you that



Computer says no.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wavyedge]
    #28658828 -

Some lm plates that have overgrown



--------------------
halfass mycology

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What is this shiny stuff running ahead of the myc? [Re: GenericHero]
    #28659167 -

Hi.

I cloned a GT and this is T1.  I took 4 different photos to try to show what I'm asking about. There is a thin "shiny" front running ahead of the growing myc.  I would appreciate help understanding what it is.

Here is the normal top view of the plate:



Here is a through view, if it helps:



Here I'm a little out of focus on purpose - if you look at the "glistening" part, you can see the shiny "thing" running ahead of the good stuff.  It is clear to my eye, but I have to hold the plate "against the light", if that makes sense, and then I see this part reflect light.  Hard to photograph.



This is as magnified as I can go:



Any ideas what this is?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28659176 -

I'm calling this LC clean. Any dissenters? Do you see something I don't? Kind regards


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Re: What is this shiny stuff running ahead of the myc? [Re: SWBZA] * 1
    #28659178 -

SWBZA said:
Hi.

I cloned a GT and this is T1.  I took 4 different photos to try to show what I'm asking about. There is a thin "shiny" front running ahead of the growing myc.  I would appreciate help understanding what it is.

Here is the normal top view of the plate:



Here is a through view, if it helps:



Here I'm a little out of focus on purpose - if you look at the "glistening" part, you can see the shiny "thing" running ahead of the good stuff.  It is clear to my eye, but I have to hold the plate "against the light", if that makes sense, and then I see this part reflect light.  Hard to photograph.



This is as magnified as I can go:



Any ideas what this is?



Congratulations. You've found the leading tips of the threads. That's where it all begins.

If you're worried about it try transfering just a bit from that part and see what you get.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Re: What is this shiny stuff running ahead of the myc? [Re: elasticaltiger] * 1
    #28659181 -

elasticaltiger said:
Quote:
SWBZA said:

Any ideas what this is?



Congratulations. You've found the leading tips of the threads. That's where it all begins.



:awethumb:

Dr. Livingston! I am :rofl:

:thanx:

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Re: What is this shiny stuff running ahead of the myc? [Re: SWBZA]
    #28659188 -

On the other hand it could also be bacteria growing slightly faster than the mycelium with the mycelium initially weakly rolling into it and then fluffing up as it grows over.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Re: What is this shiny stuff running ahead of the myc? [Re: elasticaltiger]
    #28659193 -

elasticaltiger said:
On the other hand it could also be bacteria growing slightly faster than the mycelium with the mycelium initially weakly rolling into it and then fluffing up as it grows over.



Grrrrr.... Don't go spoiling my good mood now!
:cryaboutit:

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Re: What is this shiny stuff running ahead of the myc? [Re: Tormato] * 1
    #28659560 -

Looking for feedback & critiques. blue agar is BRF, red is LME, all 2%. should i be using karo? almost all T1 plates grew tomemtose in the center until about day 10. All PE, clone was taken from a plate pin. How soon should a transfer be taken from the plate pin?


the two T2 plates have some denser spots near the donor wedge. i've noticed this happens if it doesn't easily come off the scalpel, so i'm thinking that strands landed there. thoughts on the myc?


--------------------
New or Returning Grower? Start here

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Re: What is this shiny stuff running ahead of the myc? [Re: Daeda1us] * 1
    #28660315 -

Daeda1us said:
Looking for feedback & critiques. blue agar is BRF, red is LME, all 2%. should i be using karo? almost all T1 plates grew tomemtose in the center until about day 10. All PE, clone was taken from a plate pin. How soon should a transfer be taken from the plate pin?


the two T2 plates have some denser spots near the donor wedge. i've noticed this happens if it doesn't easily come off the scalpel, so i'm thinking that strands landed there. thoughts on the myc?




The exact same thing happened to my plates. The tissue clone was super rhizo and the T1 looked exactly like yours.




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Re: What is this shiny stuff running ahead of the myc? [Re: koma23]
    #28660325 -

koma23 said:
The exact same thing happened to my plates. The tissue clone was super rhizo and the T1 looked exactly like yours.







sweet comparison, guess the difference in growth has something to do with digestive enzymes.

:takingnotes:

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Re: What is this shiny stuff running ahead of the myc? [Re: pacmanbreed]
    #28660338 -

pacmanbreed said:
koma23 said:
The exact same thing happened to my plates. The tissue clone was super rhizo and the T1 looked exactly like yours.







sweet comparison, guess the difference in growth has something to do with digestive enzymes.

:takingnotes:



Could you expand a bit on digestive enzymes please?

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Re: What is this shiny stuff running ahead of the myc? [Re: koma23]
    #28660370 -

had to stop cultivating for a while and was just studying & refreshing my memory to start over(agar).
Im no expert but that brf has vigorous & prominent - has both types of growth visible (rhizo+tememtose) to isolate.

just a guess,
brf/Grain water = more starch
LME = more maltose

Edited by pacmanbreed (02/15/24 05:46 AM)

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AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28660375 -

Fixing title.


--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pacmanbreed]
    #28660385 -

wtf are you talking about? Lol

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #28660396 -

:lol:, it just amazes me how it grows on different of nutes, will think less about it and follow KISS method instead.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pacmanbreed] * 2
    #28660400 -

lol ok that makes sense. I shouldn’t be getting on here first thing when I open my eyes in the morning 🤣

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers] * 1
    #28660464 -

Made some gnocchi (potato based) the other day, and the boil water looked thick and starchy, and since my grainwater agar has been underperforming, I figured this could be a good alternative.



I'll do 500ml, 8-10g agar and a tiny bit of sugar. I will also probably do 2 batches simultanerously at different concetrations.

Which dilutions should I test? Full gnocchi water, 1/2, 1/4?


--------------------
I call them Huskies cause they Mush!


The Official Wild Cubensis Thread
Instant Pot Agar
Gnocchi Water Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 1
    #28660540 -

This is an interesting read.

More or less says go full flavour with that potatoe water. I’d try it at full concentration and diluted 1:2. See which performs best. I suppose it depends what species you’re putting to said agar though as well.

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