SingularFusion said:

yeah I have long been wanting to see a thread with some sort of visual comparison of the same culture on various agar mixes. I see here locally the difference in morphology of the mycelia can be quite notable dependent on the mix. I have developed a fondness for wheat malt extract with a bit of yeast in recent times but my sterile work is pretty rusty at the minute sadly. Must take some time to re-dedicate myself

So at the minute I have been working up this Semperviva which has been a real bitch. Here is the latest iteration, a T3



I have returned to the old paper print and started again many times, going back to like november. I threw out a lot of grain jars that start from plates fairly like this and look good in the jars until a shake or two, and then some sort of sweaty socks/armpit smelling white mold/yeast takes over. I got so frustrated with it I even spawned a jar of it just to make sure I wasn't wrongly judging my jars, promptly molded also and grain did not jump off or recover on substrate

That may be the cleanest looking plate I produced to date...

What does anyone running Semps regularly think about the quality of that plate please?

It looks good to me in person although it looks a little thin or something? I rather not waste any more resources on this if it is garbage and try and source some fresh spores or something

My other work on other cubes, cyan and bispo looks acceptable, but I just can't get these Semps right

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chris77 said:

playa x fiji f6 germ plate

from where would you transfer and why?

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SWBZA said:

Porn plate, because why not



T2 of clone of this chap:



Went to grain 5 days ago:



Hoping it will be as clean on the grain as it looks to me on the agar.

BTW, I'm still getting used to Spitball's grain prep tek.  Those grains look dry to me!  Following the Cult advice of following a tek to the T several times before asking questions about it.  At least the myc seems to be jumping off the agar wedge, so let's hope there is enough moisture inside the grains.  Time will tell.

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elasticaltiger said:

SWBZA said:

BTW, I'm still getting used to Spitball's grain prep tek.  Those grains look dry to me!  Following the Cult advice of following a tek to the T several times before asking questions about it.  At least the myc seems to be jumping off the agar wedge, so let's hope there is enough moisture inside the grains.  Time will tell.

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Those grains are perfect.

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Thanks, Tiger.  New processes always feel weird, but what felt "right" didn't work, eh, so this is the "new right"   Looking forward to a healthy jar to take to bulk soon.

SingularFusion said:

Isn't it really only effective against bacteria and stuff that runs fast on sugar media? I mean if mold is running alongside the culture somehow I assume that carries over onto water agar still?

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