|
krock
Krockodile

Registered: 12/18/19
Posts: 154
|
Interesting agar recipes from the Radical Mycology book 8
#26795122 - 06/30/20 05:56 PM (3 years, 6 months ago) |
|
|
I recently posted some agar recipes from the Radical Mycology book in the agar envy thread and D3 suggested I should put it in a new thread so that it can be found easily with the forum search.
This is how the recipes are presented in the book:
I made a transcript for better search indexing:
Agar Media Recipes
Unless noted, all agar recipes start with 500 milliliters of water and 10 grams of agar. Never use the following antifungal ingredients in media recipes: coconut, garlic, pomegranate, cinnamon, cloves, curry, and cilantro.
COMMONLY USED MEDIA
Malt Extract Agar (ME) • Dry, light malt (barley) extract - 10 g
Malt Yeast Extract (MYA) • Malt - 10 mL • Yeast - 1 mL
Malt Extract with Activated Carbon (MEAC) • Malt - 10 g • Activated carbon - 1 g
Malt Extract with Peptone (MEP) • Malt - 10 g • Peptone - 1 g
Malt Extract with Yeast and Peptone (MYAP) • Malt- 10 g • Yeast - 1 g • Peptone - 1 g
Malt Extract with Yeast, Peptone, and Activated Carbon (MEPYAC) • Malt - 10 g • Peptone - 1 g • Yeast - 1 g • Activated carbon - 1 g
Malt Extract with Milo (MEM) • Malt- 10 g • Ground milo - 10 g
Malt Extract with Corn (MEC) • Malt- 10 g • Ground corn - 10 g
Malt Extract with Honey (MEH) • Malt- 10 g • Honey- 16 g
ATTC Medium 597 • Malt - 3.75 g • Yeast - 0.3 g • Peptone - 0.5 g
MYAP with Multi-vitamin (MYMP) • Fresh ground crickets - 8 g • Multi vitamin - 0.01 g • Peptone - 1 g • Yeast - 1 g • Malt- 10 g
Add multi-vitamin after sterilization
MYAP with elements (MYPMCZ) • Yeast - 1 g • Peptone - 1 g • Calcium - 0.01 g • Magnesium - 0.01 g • Zinc - 0.01 g
MYA with Cordyceps sinensis (CS4MY) • Malt - 3.75 g • Yeast - 0.3 g • Ground Cordyceps sinensis 4 - 10 g
Potato Dextrose Agar (PDA) • Diced and boiled potatoes - 150 g OR Potato starch - 10 g • Dextrose - 7 g
In 500 ml of water, boil 150 g of potatoes. Strain out the solids, bring the solution back to 500 ml with water, then add remaining ingredients. Five grams of potato flakes can be used as a substitute.
Potato Dextrose Agar (PDA) • Dextrose or Honey/Corn syrup - 7 g or 10 ml • Diced potatoes - 150 g
Potato Dextrose Yeast Agar (PDYA) • Dextrose or Honey/Corn syrup - 7 g or 10 ml • Diced potatoes - 150 g • Yeast - 1 g
Potato, Ammonium citrate, Thiamin Agar (PAT) Cordyceps militaris is known to fruit from this media when used to inoculate brown rice.
• Potato broth - 100 ml • KH2PO4 - 1 g • MgSO4 - 0.5 g • Peptone - 1.5 g • Ammonium citrate - 0.5 g • Dextrose - 15 g • Thiamine (Bl vitamin) - 0.025 g
Potato Skin Dextrose Agar (PSDA) • Dried potato skins - 10 g • Dextrose - 7 g
Barley Flour Malt Extract Agar (BFMA) • Barley flour - 40 g • Malt extract - 2 g • Yeast extract (optional) - 1 g
Oatmeal Agar (OA) • Filtered, cooked oatmeal - 30 g
Cornmeal Malt Agar (CMMA) • Cornmeal - 10 g • Dextrose - 7 g
Dog Food Agar (DFA) • Dried, ground dog food - 10 g • Amaranth flour - 10 g • Dextrose or malt extract - 2 g
Amaranth Soy Agar (ASA) • Amaranth flour - 20 g • Soy flour - 20 g
EntheoGenesis Agar (EA) • Amaranth flour - 20 g • Soy flour - 20 g • Brown rice flour - 10 g • Potato flour - 10 g • Malted barley - 2 g
Moonflower's Rice Malt-Alfalfa-Brewer's Yeast Agar (MRMABYA) Soak 1 cup of alfalfa and 2 cups of rice in 1.5 quarts of clean water for 2 hours at room temp. Stir occasionally. Filter out solids. Pour a packet of baker's yeast in water. After 30 minutes, filter out the solids. Combine liquids and add 1 tablet of crushed dolomite. Supports luxuriant mycelial growth and germinates spores.
Spore Germination Agar# 1 • Cornmeal - 10 g • Dextrose - 1 g • Yeast - 0.5 g
Spore Germination Agar #2 • Cornmeal - 10 g • Dextrose - 3.5 g • Sucrose - 5 g • Yeast - 0.5 g • KH2PO4 - 0.5 g
Spore Germination Agar #3 • Cornmeal - 10 g • Malt - 0.8 g
Spore Germination Agar #4 • Cornmeal - 10 g • Glucose - 1 g • Sucrose - 1.5 g • Yeast - 0.5 g
JUICE / BROTH MEDIA
VB Juice Agar (VBA) • CaCO3 - 1.5 g • V8 Juice - 100 mL
Beef Broth Agar (BBA) • Beef broth - 20 mL • Malt - 5 g • Yeast - 1 g
Chicken Broth Agar (CBA) • Chicken broth - 20 mL • Malt - 5 g • Yeast - 1 g
Butternut Squash Soup Agar (BNSA) • Butternut squash soup - 20 mL • Malt - 5 g • Yeast - 1 g
Beef Yeast Agar (BYA) • H2O - 300 mL • Yeast - 2 g • Beef broth - 200 mL
Malt with Activated Carbon and KOH (R7) • Epsom salts - 0.25 g • Activated carbon - 1.3 g • Malt - 12.5 g • Humus - 2.5 g • KOH 1 % - 20 mL
CHEMICAL MEDIA
Organic Medium Agar (OMA) • Glucose - 5 g • Peptone - 0.5 g • Yeast - 0.05 g • KH2PO4 - 1 g • MgSO4 • 7H2O 0.15 g • OR MgSO4 - 0.075 g
Czapek's Medium • NaNO3 - 1.5 g • K2HPO4 - 0.5 g • MgSO4 - 0.25 g • KCl- 0.25 g • FeSO4 - 0.0187 g OR Fe SO4 • 7H2O - 0.0094 g • Dextrose - 15 g
Used for the isolation and culture of saprobic soil microorganisms.
ANIMAL RELATED MEDIA
Blood Worm Agar (BW) • Blood worms - 5 g
Krill Agar (KA) • Krill - 10 g
Fish Flake Dextrose Agar (FDA) • Fish flakes - 3 g • Dextrose - 10 g
Meal Worm Agar (MWA) • Meal worms (ground) - 10 g • Dextrose - 3 g • Yeast - 1 g
Fishy Wax Agar (FWA) • Bee's wax - 1 g • Krill, tubiflex worms, and fish flakes - 3.3 g each
MISCELLANEOUS MEDIA
Manure Agar • Ground horse manure - 50-60 g
Boil manure in water for 10 minutes, let sit for 16-20 hours, filter out solids, bring back to 500 mL and add agar.
Compost Agar Air-dry and then grind hot compost obtained during its peak state. Mix 50 g of this dried compost powder to 750 mL of water. PC for 1 hour at 15 psi. Filter twice through cheese cloth. Bring volume up to 500 mL. Add 10 g agar and PC as normal.
Complete Media (CM) • Sucrose - 15 g • Ammonium tartrate (NH4 -) - 2.5 g • Ammonium nitrate (NH4NO3) - 0.5 g • Magnesium sulfate heptahydrate • (MgSO4 • 7H2O) - 0.25 g • Sodium chloride (NaCl) - 0.5 g • Calcium chloride (CaC1 2 ) - 0.065 g • Yeast - 0.5 g • Monopotassium phosphate (KH2PO4) - 0.5 g
Richard's Medium (forT. harzianum) • Potassium nitrate - 5 g • Monopotassium dihydrogen phosphate - 2.5 g • Magnesium sulphate - 1.25 g • Ferric chloride - 0.01 g • Sucrose - 25 g
wMY Agar (For Protostelid slime molds) • Malt extract - 0.01 g • Yeast extract - 0.01 g • Dibasic potassium phosphate - 0.375 g
Distilled Water Agar (DWA) This formula should be made solely with dis- tilled water and agar.
Sea Water Agar (SWA) • H2O - 125 mL • Agar - 10 g • Sea water - 375 mL • Beef extract - 5 g • Peptone - 5 g
Dissolve the beef extract and peptone in warmed HP. Adjust the pH to 7.8 and boil for 10 minutes. Readjust pH to 7.3 and add agar.
Honey Agar (HON) • Honey- 16 g
Beet Root Agar (BRA) • Beet root supplement - 3 g • Malt - 10 g
Peaches Malt Agar (PMA) • Canned peaches mash - 10 g • Dextrose - 7 g
Spirulina Agar (SPA) • Spirulina supplement - 3 g • Malt - 10 g
Nori Agar (NorA) • Nori- 3 g • Malt - 10 g
Brine Agar (BA) • Brine - 10 g
Bean Soup Agar (BSA) • H2O-400 mL • Agar - 10 g • Bean soup - 100 mL
Carrot Malt Agar (CMA) • Carrot mash - 20 g • Malt - 5 g
Sunflower Seed Agar (SFS) • Ground sunflower seeds - 10 g
Almond Agar (AA) • Ground almonds - 10 g
Edited by krock (07/01/20 04:15 AM)
|
tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 54 minutes, 7 seconds
|
Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#26795264 - 06/30/20 06:55 PM (3 years, 6 months ago) |
|
|
|
Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 7 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: tripdawg420]
#26795520 - 06/30/20 08:35 PM (3 years, 6 months ago) |
|
|
Thanks, OP.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
|
mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 16 hours
|
Re: Interesting agar recipes from the Radical Mycology book [Re: Tight Lunchbox]
#26796037 - 07/01/20 12:53 AM (3 years, 6 months ago) |
|
|
This is pretty cool. My memory is shot and I frequently find myself looking up how much MEA to use per litre
The germination blends are interesting. Sometimes spores are so stubborn, I'm willing to try anything
--------------------
 Amateur Mycologists United AMU Q&A
|
sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
|
Re: Interesting agar recipes from the Radical Mycology book [Re: mushpunx] 1
#26796349 - 07/01/20 06:46 AM (3 years, 6 months ago) |
|
|
Imagine your wife catching you boiling 50 grams of horse shit on the stove lmao.
|
krock
Krockodile

Registered: 12/18/19
Posts: 154
|
Re: Interesting agar recipes from the Radical Mycology book [Re: sandman420]
#26796412 - 07/01/20 07:24 AM (3 years, 6 months ago) |
|
|
Quote:
Imagine your wife catching you boiling 50 grams of horse shit on the stove lmao.
LOL!!
|
Jus
Stranger



Registered: 09/27/19
Posts: 44
Last seen: 6 months, 8 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#26796427 - 07/01/20 07:38 AM (3 years, 6 months ago) |
|
|
Wow, some of these are so much work compared to others.
That butternut squash soup agar sounds delicious though.
|
amy437737
Ranger

Registered: 05/01/21
Posts: 2
Loc: LA
Last seen: 2 years, 6 months
|
Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#27293016 - 05/03/21 09:06 PM (2 years, 8 months ago) |
|
|
Thank you so much for this post. I am researching agar now to do a test and this was so helpful.
-------------------- -Amy
|
Benson
The Kidd ⭐


Registered: 09/29/20
Posts: 837
|
Re: Interesting agar recipes from the Radical Mycology book [Re: amy437737]
#27293022 - 05/03/21 09:09 PM (2 years, 8 months ago) |
|
|
Dope, thanks for transcribing it too
-------------------- [||||||||||||||||||||||||||||||||||||] [||||||||||||||||||||||||||||||||||||] [||||||||||||||||||||||||||||||||||||]
|
amy437737
Ranger

Registered: 05/01/21
Posts: 2
Loc: LA
Last seen: 2 years, 6 months
|
Re: Interesting agar recipes from the Radical Mycology book [Re: Benson]
#27294954 - 05/05/21 02:49 AM (2 years, 8 months ago) |
|
|
I made a few of these including the horse manure agar. Testing seven different syringes on LME, PDA (PDA is a Philly GT recipe), BRF, a mix of extras, HorsePooH20 and GrainSoakWater agar.
Sterilized in an All American and poured in a SAB. I'm a noob. Got no idea what I am doing.
-------------------- -Amy
|
fungilove
Stranger
Registered: 01/26/21
Posts: 265
Last seen: 8 months, 16 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: amy437737]
#27364637 - 06/26/21 10:41 PM (2 years, 6 months ago) |
|
|
I'm wondering what came of this test?
|
B H O



Registered: 03/17/13
Posts: 675
Last seen: 1 year, 21 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: fungilove]
#27364640 - 06/26/21 10:47 PM (2 years, 6 months ago) |
|
|
Quote:
fungilove said: I'm wondering what came of this test?
Right!? It’s been a month and no feedback?
|
kanemush
Grumpy


Registered: 12/07/17
Posts: 1,502
Last seen: 8 months, 28 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: B H O]
#27364661 - 06/26/21 11:12 PM (2 years, 6 months ago) |
|
|
interesting I think I may try some of these out thank you for posting.
|
multifractal
Student


Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
|
Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#27365739 - 06/28/21 12:15 AM (2 years, 6 months ago) |
|
|
lol brine agar? these are cool. I mixed up agar using the cornmeal/dextrose/yeast spore germination recipe today. I'll see how it goes.
Edited by multifractal (06/28/21 12:16 AM)
|
doubleD
Stranger


Registered: 12/08/15
Posts: 424
Last seen: 7 days, 7 hours
|
Re: Interesting agar recipes from the Radical Mycology book [Re: multifractal]
#27365839 - 06/28/21 04:35 AM (2 years, 6 months ago) |
|
|
damn, good luck amy! thats a lot to keep track of i may try something here if i hear good things about a recipe, but i usually use what i got. about 12.5g agar for 25 plates and then a couple gs of lme and a splash of nutritional yeast, and sometimes tea as a water substitute
-------------------- teach your kids to recognize and despise all the propaganda
|
fungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,028
|
Re: Interesting agar recipes from the Radical Mycology book [Re: doubleD]
#27369419 - 06/30/21 06:29 PM (2 years, 6 months ago) |
|
|
Thanks OP!
|
0ptiquest
Stranger


Registered: 07/05/20
Posts: 509
Last seen: 7 days, 11 hours
|
Re: Interesting agar recipes from the Radical Mycology book [Re: fungusul]
#27397980 - 07/22/21 12:37 PM (2 years, 6 months ago) |
|
|
Someone have tried the spore germination recipes? Will try some dextrose in my next plates because i have it.
|
multifractal
Student


Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
|
Re: Interesting agar recipes from the Radical Mycology book [Re: 0ptiquest]
#27398192 - 07/22/21 04:13 PM (2 years, 6 months ago) |
|
|
Yes! I've tried the cornmeal germination recipe to great success; I can't stop recommending it to people. I haven't conducted any formal experiments but just anecdotally germination seemed to occur far more quickly. I made 3 germination plates with subtropicalis using a modification to the recipe in this book and 3 with my usual BRF/yeast recipe. The three cornmeal dishes all showed signs of germination within just 2 days while the quickest of the 3 BRF plates germinated in 4 days and the slowest in 8! The same speed was noticed for some APE and a 1 year old mexicana print. Also anecdotally, they (the cornmeal plates) seem a little more prone to contamination but that might just be a coincidence.
I used the following:
10 g cornmeal 1 g dry active yeast 1/4 teaspoon light karo syrup 6 g agar powder
to 500 ml of water.
I'd love for some others to try this out and share their experiences.
Edited by multifractal (07/22/21 06:14 PM)
|
sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
|
Re: Interesting agar recipes from the Radical Mycology book [Re: multifractal]
#27398316 - 07/22/21 05:50 PM (2 years, 6 months ago) |
|
|
The faster cornmeal shows more contams? That makes sense if it works better for germinating.
|
multifractal
Student


Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
|
Re: Interesting agar recipes from the Radical Mycology book [Re: sandman420]
#27398343 - 07/22/21 06:18 PM (2 years, 6 months ago) |
|
|
Yeah, the ones that showed contamination had a far greater variety of contaminant organisms than I usually see when a plate goes bad. The contaminations also showed themselves within the first 12 hours and i saw no additional ones after that.
Edited by multifractal (07/22/21 06:19 PM)
|
brighteyesburner
Eager Beaver

Registered: 08/16/21
Posts: 102
Loc: MN
Last seen: 2 years, 1 month
|
Re: Interesting agar recipes from the Radical Mycology book [Re: multifractal]
#27435812 - 08/20/21 10:33 AM (2 years, 5 months ago) |
|
|
This is AWESOME! I've asked around several mycology labs and they all seem to use Potato Dextrose Agar (1X or 2X). Is there a general "Best"?
-------------------- "My father says that almost the whole world is asleep... He says that only a few people are awake and they live in a state of constant total amazement." -Patricia (Joe Versus the Volcano)
|
bradburydoom
wanderer

Registered: 06/17/16
Posts: 38
Last seen: 1 year, 2 months
|
Re: Interesting agar recipes from the Radical Mycology book [Re: multifractal]
#27884603 - 07/31/22 08:26 PM (1 year, 5 months ago) |
|
|
i know this thread is a bit old...but not too old.
what type of cornmeal are you using? how finely ground is it? just ordinary medium grind?
i may give this a shot and compare to my normal, agar, potato starch, honey recipe.
Edited by bradburydoom (07/31/22 08:29 PM)
|
BluePetri
Back Again
Registered: 01/10/23
Posts: 13
Last seen: 7 months, 13 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: bradburydoom]
#28189029 - 02/15/23 04:51 PM (11 months, 6 days ago) |
|
|
Apparently it is too old 
Just wanted to note that I discovered this book recently, did a search for the title and "pdf" and it's apparently readily available. Seems interesting.
|
balisonggrow
New Grower


Registered: 08/05/23
Posts: 16
Last seen: 4 months, 2 days
|
Re: Interesting agar recipes from the Radical Mycology book [Re: krock]
#28421880 - 08/05/23 01:21 PM (5 months, 21 days ago) |
|
|
Just finish the book, as a beginner it has given me a lot of motivation. I'm trying the honey-agar for the first time now, will update how it goes
-------------------- New grower
|
|