3.A.M said:

…..
Pp5 containers have a higher failure rate for me and lower visibility so they’re really not practical, and glass is so unaffordable given how much I’d need to get by so I’d love to hear your alternate suggestions as this is something myself and definitely others here have often discussed before with no clear solution, and honestly the waste is minimal when compared to say, literally 99% of industries on any scale out there there’s bigger and better drums to bang on bro and my guilt has bigger fish to fry.

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morningWood said:

I have some swabs that I really want to germinate. I haven't been having luck with swabs. What's your germination agar recipe?? Going to brew some up today. Thanks.

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Smellyhobbit said:

I have decided to use double the disposable plastic plates in retribution.

In all seriousness, despite low visibility, it’s less expensive and very easy to use 1/2 pint jars as petris. No, you can’t see super well into them, but you’ll be opening them to do work anyway. That’s an actual practical glass solution if you don’t want to use plastic 👍

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100mm glass plates are $1.70ea in a 20pk at the major online retailer. Close to the same price as half pints in my area.

LadysKnight said:

Smellyhobbit said:

I have decided to use double the disposable plastic plates in retribution.

In all seriousness, despite low visibility, it’s less expensive and very easy to use 1/2 pint jars as petris. No, you can’t see super well into them, but you’ll be opening them to do work anyway. That’s an actual practical glass solution if you don’t want to use plastic 👍

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100mm glass plates are $1.70ea in a 20pk at the major online retailer. Close to the same price as half pints in my area.

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Smellyhobbit said:

What I do is get a syringe of sterile water and put a single drop on the swab. The cotton will absorb it. Then you streak.

You can tease out cotton fibers and jam those into agar, too. Some people have success with that. I personally don’t.

Stubborn or very dirty swabs I will hydrate and then stick whole into a BRF puck. That’s PF tek substrate at the bottom of a half pint jar about 1/4 thick. That has a very high success rate for hard to germ swabs and syringes

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Luminous7 said:

Smellyhobbit said:

What I do is get a syringe of sterile water and put a single drop on the swab. The cotton will absorb it. Then you streak.

You can tease out cotton fibers and jam those into agar, too. Some people have success with that. I personally don’t.

Stubborn or very dirty swabs I will hydrate and then stick whole into a BRF puck. That’s PF tek substrate at the bottom of a half pint jar about 1/4 thick. That has a very high success rate for hard to germ swabs and syringes

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Oh good ideas, Im recently being forced into learning how to rehydrate stubborn spores. Thinking of the easiest way as i cant confirm my swabs are 100% clean, prob are but...

I feel sketched out to just leave a swab soak in sterile water for a night, but it seems that would work.

Is sucking up boiling water into a syringe a few times good enough for sterilization?

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Smellyhobbit said:

LadysKnight said:

Smellyhobbit said:

I have decided to use double the disposable plastic plates in retribution.

In all seriousness, despite low visibility, it’s less expensive and very easy to use 1/2 pint jars as petris. No, you can’t see super well into them, but you’ll be opening them to do work anyway. That’s an actual practical glass solution if you don’t want to use plastic 👍

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100mm glass plates are $1.70ea in a 20pk at the major online retailer. Close to the same price as half pints in my area.

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Oh i didn’t know that. Are they good quality?

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