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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 5 hours, 28 minutes
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Re: Ask Quick Questions Here [Re: Rotnpins] 1
#27829128 - 06/20/22 07:02 PM (1 year, 7 months ago) |
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Quote:
Rotnpins said: Have any pictures?
I didn’t see this reply, my bad.
Here’s the one that got the most growth from spore. It was thin, translucent, and hasn’t changed in I guess a week or so?

Edit: as you can see by the sharpie, this is also made from a dirty LC. It’s the only one of its kind I made, so it might not be the best example. Others are spore and one is a colonized grain. I can take pics of those if that would be helpful, but I can also just let them go for a few weeks and see if they do anything.
Edited by Smellyhobbit (06/20/22 07:07 PM)
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
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Quote:
CowsPoopShrooms said: . . Quick Question: Can these single use needles (18g) be re-sterilized in a PC? Yes, they say, "single use". Can they be reused? Can they stand the heat of a PC? If yes, What's a good PC time?

Those pictured were used to inoc PF Tek jars.
You really don't need to pressure cook needles just to push unsterilized spores through them. Put the needle on the syringe, Bring water to a boil in a frying pan, Draw boiling water into the syringe a few times and squirt it in the sink, on the last draw leave the water in the syringe, place on the cap, and wait for it to cool.
Always flame your needles before inoculation and you'll be as good as any spore syringe you buy from a vendor.
Here a tek for it https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681
Edited by rumfor69 (06/24/22 07:57 PM)
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AKAMacC
Stranger
Registered: 06/26/22
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So give it to me straight. Am I screwed? Should I throw this brick an try again or is there hopes to save?
Everything was great till my first flush. After I harvested for the first time. I soaked the bricks in distilled water for 12 hours each. It's been a week an they look like this.
For the record. Total noob here. This is my first attempt. Appreciate all the help.


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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Ask Quick Questions, Get Quick Answers [Re: AKAMacC]
#27837161 - 06/26/22 08:12 AM (1 year, 6 months ago) |
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Quote:
AKAMacC said: So give it to me straight. Am I screwed? Should I throw this brick an try again or is there hopes to save?
Everything was great till my first flush. After I harvested for the first time. I soaked the bricks in distilled water for 12 hours each. It's been a week an they look like this.
For the record. Total noob here. This is my first attempt. Appreciate all the help.



I'm not sure if they can be saved, id wait for a second opinion,. but if that picture is in your fruiting chamber you should cut back your foil. It should be barely larger than the cake, that way the perlite is able to do its job properly.
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 3 days
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Quote:
CowsPoopShrooms said: . . Quick Question: Ok, I'm hoping this is a quick Question/Answer, How do I tell if my agar plate(s) are finally "clean"? Is it by the growth/presence of a monoculture or what? Sorry, but I don't think I've found an actual explanation at a nOOb level. I'm on T3 and getting ready to do T4s. I have 1 that is very close, IMO, to a "monoculture". What am I looking for?
Some examples......
This is a T1 from multispore. There are obviously several sectors here.... several cultures growing on the same plate. To my eyes, this looks clean, but there is some risk there - perhaps the area growing around 9:00 is contaminated by some other fungus. Again, it's probably clean but some people (including myself if I've already tried growing a variety out and had contamination issues) might take more transfers until they don't see sectoring like this:

This is someone else's photo. I post this here to give an example of bacterial contamination. You can see it on the bottom. Note the sharp edges and yellowish color vs the fuzzy edges and whiter color of the mycelium above. Bacterial contamination is often round.

Bacterial contamination again. Notice the round spots with sharp edges:

Another bacterial, with some mycelium growth mixed in:

More bacteria:

You want to avoid anything of an odd color too... small black, brown, green, etc spots are contamination. I don't have any pics of that handy.
Like the first example, this is too uneven for me. I wouldn't send this:

Here are some examples of nice looking plates:

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albird71
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
#27842642 - 06/29/22 04:27 PM (1 year, 6 months ago) |
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Not sure if this worked or not? But as you can see, the tall skinny one has the point on the cap. The short fat one is more round. Both are in the process of drying so I don't have a fresh picked photo.
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Screwup
Googles your dumb questions


Registered: 01/27/22 
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Re: Ask Quick Questions, Get Quick Answers [Re: albird71]
#27842647 - 06/29/22 04:30 PM (1 year, 6 months ago) |
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ONE MORE QUICK QUESTION? Can I send it or no?
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
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Re: Ask Quick Questions, Get Quick Answers [Re: albird71] 1
#27842746 - 06/29/22 06:11 PM (1 year, 6 months ago) |
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Quote:
albird71 said:

Not sure if this worked or not? But as you can see, the tall skinny one has the point on the cap. The short fat one is more round. Both are in the process of drying so I don't have a fresh picked photo.
I'm pretty sure that the one on the left is B+
Ive gotten pointy caps from both... but I've had way more B+ look like that 
The ones in this picture are GT with pointy caps, but the B+ have more of a nipple like yours.. can't find any decent pics tho..

Just I could be wrong
Edit: here's some B+
 
idk,.. smellyhobbit's right.. just mix it all together
Edited by Rotnpins (06/29/22 06:26 PM)
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DERRAYLD
Constructus

Registered: 05/13/02
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup] 1
#27843310 - 06/30/22 03:55 AM (1 year, 6 months ago) |
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Quote:
Screwup said: ONE MORE QUICK QUESTION? Can I send it or no?

I wouldn't send that yet, still lots of different growth there.
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san pedro guy
Captain



Registered: 10/22/17
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As an example: the myc was growing on the other side but you can see the grains on the bottom side of the plate colonizing already as it’s barely passed the edge on the opposite side

Always thought it grew only on top since agar was always referred to as “2d grow medium”
But good to know, thanks hindsight!
-------------------- Noob Grow Along 2022
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mushzen
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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins]
#27847483 - 07/03/22 09:32 AM (1 year, 6 months ago) |
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Hey, new grower here!
Just had my first brown oyster flush ever and I'm getting mixed results so I was hoping it would be an easy fix.
Short summary - 3x3m plastic sheet/wood frame enclosure, cycling air 4 times per hour and maintaining humidity in the 85-95% range, small fan to ensure circulation, 1000 lumens 6500K light.
I'm getting very small caps (~2in diameter) and the first flush was around 250grams on 3kg blocks and I'm not really sure why. Attached pictures, let me know if you see anything obviously wrong with the setup or growing conditions.
Thanks a bunch in advance!




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wild_aussie
Punk rocker til I die



Registered: 01/05/22
Posts: 1,000
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27857086 - 07/10/22 09:40 AM (1 year, 6 months ago) |
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 So this is pe on oats what should I do here? Fae or case or.... Idk thanks
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Vibetyme
Smoke 1



Registered: 06/08/21
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Re: Ask Quick Questions, Get Quick Answers [Re: cozmyc] 1
#27859790 - 07/12/22 10:25 AM (1 year, 6 months ago) |
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Quick question: does anybody know what this contam is?

 Same jar
I'm trying to narrow down where it came from.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
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Re: Ask Quick Questions, Get Quick Answers [Re: Vibetyme] 1
#27860194 - 07/12/22 05:15 PM (1 year, 6 months ago) |
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Quote:
Vibetyme said: Quick question: does anybody know what this contam is?

 Same jar
I'm trying to narrow down where it came from.
Mold, set it on fire and bury it!
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Ask Quick Questions, Get Quick Answers [Re: Cob]
#27860518 - 07/12/22 10:01 PM (1 year, 6 months ago) |
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@shroomduck they can get in your fruits . Whatever you get from those tubs I would use for tea. But to each his own ...
& This is my work table btw  Bought a sheet of sheet metal threw that on a old wooden work bench And put my hood on top k.i.s.s
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Screwup
Googles your dumb questions


Registered: 01/27/22 
Posts: 6,293
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Re: Ask Quick Questions, Get Quick Answers [Re: Grogu] 1
#27861052 - 07/13/22 10:49 AM (1 year, 6 months ago) |
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Well…back at it again. Millet PC @15 for 1.5HRs inoculated with Fahts PE on 6/24. I have shaken twice and here is where I am at….so I shake again this time or just leave it be and see if it ever fully colonizes? There’s a larger clump of colonized millet in the middle I remember from the last time.
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Culliganman360
Stranger



Registered: 06/05/19
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
#27861060 - 07/13/22 10:54 AM (1 year, 6 months ago) |
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I would let that grow out untill the points of mycelium colonize at least 3 layers/rows of the surrounding grain bro then shake her
This is a little bit longer than I usually wait for a shake but good example of shake worthy You want the mycelium to have good footing before you shake , if u shake too soon then it will be counterproductive
Edited by Culliganman360 (07/13/22 10:57 AM)
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
Last seen: 9 months, 3 days
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Quote:
InfiniteLupercal said: Hello!
So quick cultivation background; I have done quite a few BRF cakes with pretty good success. Ready to take my first dive into bulk growing. Already have a grain jar almost totally colonized. However, the spawn colonization has slowed or possible halted (inoculated about a month ago). My suspicion is too much liquid in the jar (I used liquid culture and was a little overzealous on the amount). My question is; with 75-80% of the grain covered in beautiful white mycelium, is there a way to utilize what HAS colonized, and safely get rid of the uncolonized grain? Or does it matter, and should I just put the loose uncolonized grain in with my bulk substrate and not worry about it? Sorry if this is overly vague or broad, or has been touched on anywhere. Just figured for a first post this wouldn't be too bad?!?! Thanks for any help!!!
(I can snap a picture and attach it if that would help?!)
Pics might help give a better answer, depending on what the pics show. We might be able to identify some contamination in the jar depending on how it looks. If the jar is contaminated, most people don't bother trying to salvage portions of the jar because the success rate is rather low. You are best to just toss them and start over. If you REALLY want to salvage them and you don't have the culture backed up on an agar plate, I would suggest sterilizing a blade, letting it cool, then pulling out a couple of grains that look clean from the top and putting them on agar. Alternately you wrap a spoon in foil then bake it at 400F for 30 mins. Let it cool, then use it to scoop a tablespoon or two of grains from the top of the jar and dump those into a new clean jar of spawn and shake. That second option is likely to have a low success rate though. And either option should be done in your SAB or in front of your hood. Again, most of us just toss bad jars. If you have a yard, toss the grains under a bush and cover it with Blak Cow, compost, or coir and you are likely to get some good mushrooms outside:

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InfiniteLupercal
Newb

Registered: 07/14/22
Posts: 13
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
#27862377 - 07/14/22 11:20 AM (1 year, 6 months ago) |
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Good to know! Thank you for the info! Here are the pictures. Second one is to show the excess liquid culture that pooled on the bottom. The brown on the mycelium is from that liquid. Otherwise, I don't think (I am probably wrong) there is any sign of contamination?

-------------------- "You will try," he said. "But today you face the Emperor's sons and his warriors. We are the Luna Wolves, and this Legion is the anvil upon which you will be broken."
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RoyalKingdom
Stranger

Registered: 07/17/22
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Last seen: 1 year, 5 months
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Hello fellow hunters. Q question - should I wait or should I start fruiting? Got a patch on top of sub which is not colonizing for about a week. No show of other contams.
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