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NextShop:  Spores for European Microscopy  APE Liquid Culture For Sale  Bulk Cannabis Seeds  All-In-One Bags That Don't Suck  Liquid Cultures  Unfolding Nature: Being in the Implicate Order  Bulk Substrate |
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 bigfootscreepyuncl Stranger   Registered: 11/15/20 Posts: 3,736 Loc: Gamehenge |
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I'm just making sure you understand what LAGM is all about here, incase you don't know..LAGM is all about spores to agar, not cakes (the brown rice made me think you were doing PF TEK)... Originally LAGM was started to show cultivators new and seasoned alike that agar is nothing to be afraid of and that it's actually pretty damn easy! Just wanted to make sure you're running agar not cakes! If you were planning to run cakes because you've never played with agar hit me up and I'd be happy to give you some good links to agar TEKs or if you're desperate I'm sure we could work a way out for you to get some ready to use plates... Happy LAGMing, I look forward to seeing what kind of weirdo B+ you end up getting 
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bigfootscreepyuncl Stranger Registered: 11/15/20 Posts: 3,736 Loc: Gamehenge |
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Goddamnit here's page 2 that I was waiting for
-------------------- 5318008
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 edixo Fun Guy  Registered: 12/21/23 Posts: 100 Loc: Noosphere Last seen: 1 year, 4 months |
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Wait, so it's ONLY to agar, not beyond that? I was planning on doing like 10 plates with a noc loop I'm gonna build. Edited by edixo (12/27/23 02:13 AM)
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bigfootscreepyuncl Stranger Registered: 11/15/20 Posts: 3,736 Loc: Gamehenge |
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No no no no it's absolutely beyond the plate. I'm hammered so I may not have explained it well at all...in fact I'm sure I didn't.. The whole thing is that you have to go spore to agar to grain to whatever..but your grow has to start spore to agar. You said MSS and brown rice so I was worried you were just shooting spores into cakes. My bad! Fuck yeah, noc some plates and let 'er rip!! My inoculation loop is DIY to and it works like a champ!  Even kept a picture of it for this exact drunken moment. Much foresight. Such wow! -------------------- 5318008
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edixo Fun Guy Registered: 12/21/23 Posts: 100 Loc: Noosphere Last seen: 1 year, 4 months |
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Oh fuck no. I hope I will never have to shoot spores into grain again. I've edited my post to make it clear I am gonna do spores to agar. :P
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AndImTheHighOne Stranger  Registered: 12/06/23 Posts: 64 Last seen: 1 year, 4 months |
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Might as well sign myself up for this since I'm new and I'll be doing pretty much everything from spore to start 2024. All will be done with vendor-supplied MSS unless otherwise noted
I'll be growing (in no particular order): Hanoi, Tosohatchee, I'm still waiting for my first PF jars to finish consolidating, but I've been using the last two weeks to brush up on my agar skills. So even though I'm feeling very good about this upcoming year, I might not be growing mushrooms from agar at all, stay tuned! Edit: Going to be switching up a few of the varieties I'll initially be putting to agar. I got a little too excited and already used my only redboy swab (oops) and the HBP hasn't arrived yet, so those two are out for now. I'll still do Hanoi and Tosohatchee, and I would really love to grow Nats but all of this is super new to me. Fingers crossed. The SAB will be all set up for when I get home tonight LOL. Depending on what condition I'm in I might get some of this going after midnight. I will probably pick at least one more strain of cube to culture, but I'll let the inspiration come to me in the moment.  How's my setup? Lol **UPDATE 1/1** Happy New Year!! Let's Grow Some Mushrooms! OK, so I put drops to all of my germ plates this afternoon. 11 plates total, 4 cube varieties cultured, and P. natalensis. All from MSS. Went with the aforementioned Hanoi and Tosohatchee, also threw in some Golden Koh F2 and Ecuador. Now we wait!!  **UPDATE 1/4** Could... Could It Be? Well, something is happening with one of my plates. When I first glanced at this my heart sort of sank because I was pretty damn sure it was bacterially contaminated. Oh well. We were bound to lose a few. But when I went back to take a picture for this post, I realized I might have been too quick to judge. I remembered that while I did try my best to shake up the Golden Koh syringe I was using, I still wound up shooting a large, visible pool of spores in the single drop I placed on the plate. I did my best to spread them out without my hand hovering over my work and just sort of crossed my fingers that everything I did was sterile because I'd hate to lose all those spores!  So still TBD what I'm working with here, but I'm a lot more hopeful about it than when I first checked. That was the only plate showing any activity so far. Staying patient! **UPDATE 1/6** MYC CHECK, MYC CHECK! Very excited to make an update this morning because we have action all over, ladies and gents. The spots I saw on the Golden Koh plate have now turned into little balls of cube mycelium.   A little hard to tell from the lighting in these pics (I tried a bunch of different setups and these were the best ones,) but those spots are nice little white, fluffy guys. Given my lack of experience, I had a hard time at first distinguishing spores germinating on agar from bacteria. Now that I have a better idea of what I'm looking at, I can pretty confidently say that the Hanoi, Tosohatchee, and Nat plates are all showing signs of germination. I have no clue what is going on with my Ecuador syringe. I inoculated three plates with it on 1/1 because I'd already put a couple drops to agar weeks ago and nothing happened. Literally nothing at all. Well, nothing is happening again. On all three plates. Bad syringe? I will probably use it on a few PF jars and see if anything happens  **UPDATE 1/9/24** First Transfer and Other Things  FIRST TRANSFER!!! A slightly overdue update today. I could have easily made my first transfer on the 7th, but I had a busy weekend and was not well-prepared to do so. The Golden Koh plates are still my superstars of the bunch, but the Nats are also taking off.  I can and probably should take a transfer from both Nat plates today. As you can see, I did take the first transfers from the best looking sections of my more advanced G. Koh plate. I'm probably going to wait until the bottom one grows out a bit more before I pick which section(s) to take. I'm open to suggestions!  The Tosohatchee and Hanoi plates are also growing nicely(ish). My Tosohatchee genetics seem to express as a lot of very thin, whispy mycelium, but it looks healthy for the most part, and much better on my T2 and T3 plates that were started last year. I can probably make transfers from any of these plates today. Unfortunately nothing to report on the three Ecuador plates (I won't take pictures of those,) and I'm soon going to give up hope on them. I saw what I thought might be germination while examining them closely last night, but I think it was just a very faint mark from where my inoculation loop touched the agar while streaking the plate. **UPDATE 1/16/24** (!) All T1s Complete (!), First Plates Contam, Bad Ecuador, and My Plan Moving Forward Alright, alright, alright we've got a lot to cover as this is my first update in A WEEK! For anyone who is following along, I certainly don't want to make you wait too long between updates and lose your interest. But what is LAGM without a bit of suspense, right?! So without further ado, I present my eight T1 transfers:  Organized by row, from top to bottom, we have: Tosohatchee, Hanoi, P. natalensis, and Golden Koh F2 Despite having quite a busy week, I didn't completely neglect my cult work. I took the remaining six transfers from my six different germ plates on 1/13 (Yes, the plates say the 12th. I was tired and mislabeled them. I haven't bothered to fix it. Sue me.Please don't) Feeling pretty good about these T1s. No satellite contams have appeared... yet. I'm seeing enough good growth that I could easily transfer away from anything that might pop up. I especially am liking the way my Nats plate on the left is looking already. Not even really sure what to make of the weird patterns on the golden Koh plates. I believe I did those back when I still did transfers like a moron and would put the myc side down on the new plate, suffocating the myc in agar. I don't do that anymore lol. I am planning to take my T2s from those plates today or tomorrow, and it looks like I'll have to be careful to take only small chunks to try to ensure I'm grabbing clean culture. Not all the news is good. If you saw my earlier update and thought the specks on my other Golden Koh germ plate looked kinda sus, you were correct to have your doubts. It was a little difficult to tell at first, but it looks like this now:  Fortunately, I already have the two T1s from the other germ plate I took back on the 8th, so I probably won't even bother with this one. It is kinda awesome looking though, isn't it? Well, there's nothing awesome about my Ecuador plates. I decided to re-inoculate them on the 11th because, why the hell not? The plates were just sitting there, still fresh and clean, and I didn't want to throw them out. I also figured it would be nice to check the syringe in question a few more times on plates I didn't care about. Well, something is growing on one of those plates now, but it sure as hell ain't Ecuador var. cubensis mycelium.  : Got me at first too because when it first started out, it looked like it could've been germination. I suppose it was, just not the helpful kind that I am still so obviously learning to identify. With T2 transfers on deck, I figure we can see how those look before making any concrete plans on how to proceed with actually growing these bad boys. Part of me is still loving the entire process of the PF Tek. I love the simplicity of the tek; I'm not in this for massive yields. It also doesn't require me to make my house smell like cooked fucking birdseed because that's the most readily available grain for my spawn at the moment. So we'll see I guess, but if my T2s look anywhere near the way they are starting, I'll probably consider doing some combination of sending a bit of that agar to grain, while also taking some clean T3s (*inshallah*) and preparing some of those plates to make LI for inoculating PF jars. I plan to work my way up to the monos at some point fam, but so far I only have experience with PF Tek, shoeboxes, and I'm currently attempting to fruit some bacterial spawn straight out of the jar. Baby steps, y'all! Thanks for reading! **UPDATE 1/22/24** No Pics, Just Pain Well, that was terrible... By far my sloppiest SAB session since the first time I tried using agar, maybe even more so. Really pretty unhappy with myself. I should have stopped when I first felt myself rushing and getting worked up by mistakes that normally wouldn't bother me so much. I decided to power through, and ended up making even more, bigger mistakes. Shocking! So I'm going to wait a couple of days for my next picture update. I'll be holding my breath that the rest of my T2s aren't completely fucked. Edit: Okay, I figured it could be of some value to type out exactly what went wrong this evening. It's helpful for me to write these things out, so that I won't make any of the same mistakes again. Also, for any other beginners following along that may have the same or similar processes as I do, this may serve as a warning or a reminder of potential pitfalls to avoid. Alright, so first I'll explain my new process for doing transfers, before I get into how I messed it up. I just recently started using a long stainless steel straw to cut perfect circular pieces from my transfer plates. This was my second time using it, and I really enjoyed it the first time for a couple of reasons: 1) I absolutely love the perfect circles; they look very nice and it's easy to tell early on if the myc is growing out uniformly on your new plate 2) There's no need to hold the transfer plate to keep it from sliding around while you cut. Just push down with the straw, scoop up with a scalpel, and move it to the new plate. The downsides: 1) You have to re-sterilize two different tools, and the straw takes a while to get red hot. Uses a lot of butane. 2) Depending on the size of the circles you cut, it may be difficult to get the circle off the scalpel and onto the new dish. 3) The end of the straw is much blunter than a scalpel blade. So if your myc is super thick/fluffy, it's hard to cut through with the straw and some will just push down the sides where the straw enters. Not the end of the world, but frustrating and confusing at first to say the least. Downsides #2 and #3 were my biggest sticking point this time, and unless I get better at that part, I almost prefer cutting squares and triangles. When I make transfers, here's everything I have in my SAB: –#3 scalpel with a #10 blade (I hear #11s are the way to go and I will have to get some of those) –Stainless steel drinking straw –A ~1.5" section of clingwrap –Surgical tray with all the previous things on it –A stack of transfer plates –A stack of fresh agar plates placed back in their original sleeve –Two metal racks (a bigger one that holds my tray and transfer plates, and which I also do my work on; and a smaller one that I keep the stack of fresh plates on) The SAB is wiped down somewhat half-assedly. Then, before going into the SAB, everything else is wiped with Lysol/Clorox disinfecting wipes or 70% iso soaked paper towels — whichever is closest to me when I start that part. Everything I'll be using inside the SAB is then covered each with a single paper towel to avoid it getting wet, then I give the entire box a good spray of tap water in attempt to clean the air of contaminants and wait ~5 minutes for the droplets to settle before starting my work. I take a plate from my transfer pile, hold it up inside my SAB to the light to confirm which sections I had previously identified as my transfer pieces, then remove a single new dish from the sleeve, take the cling wrap off the transfer dish and place it gently back on the rack. I am right-handed, so my transfer dish is to the right of my new plate so that I never have to be over the new plate while it is open. Then, I take my straw outside the SAB and flame sterilize it until glowing orange (up to 30s) and carefully re-enter my SAB with it. I then turn my tranfer plate to a spot with no myc, slightly open the plate with one hand while still holding the lid and with the other hand push the end of the straw into the agar to cool it. With the dish still open, I turn it to where I intend to take a transfer and cut a circle, then close the dish. I then take my scalpel from the SAB and flame sterilize it, carefully re-enter my SAB, turn the transfer plate back to the open spot, open the plate just enough cool my scalpel in the middle of the spot where I did the straw and then scoop the little circle onto my blade, close the transfer dish and open my fresh agar plate, and slide the piece onto the surface. This part can be pretty tricky, as the circle is no bigger than my blade and hardly any of it hangs over the side to catch onto the fresh agar. So a couple of times I've had to scrape the transfer piece myc side down and then try to flip it over. It gets the myc all wet and gooey and I hate doing it, so I try to slide it off right side up, if at all possible. I also hate carving up my beautiful, fresh agar plate, so it's sometimes a lose-lose situation. After one decent (but pretty ugly) transfer and then 3 absolute dogshit transfers in a row that wouldn't come off the scalpel, I was fit to be tied. Again, I should've stopped right there because usually I can roll with those kinda punches, but just couldn't tonight. It's also hot as fuck in my house right now with my dehydrator running and trying to keep my A/C off while I do sterile work, so I was sweating my goddamn balls off. I kept having to stop and take my hands out of the SAB to wipe sweat out of my eyes and then re-sanitize my gloves before resuming work. It was annoying. So now I'm sweating and rushing to get through the six remaining transfers I had to do, with a new process that I don't even really have the hang of yet, and it was messy yall. Filleted a few new plates and chopped up half my transfer pieces. My Nats were so fluffy and beautiful and it looks like the straw just stripped all the myc off the surface of the circle and down the sides, so that's cool. Lesson learned is don't rush!! And if you feel like you should stop while you're ahead, do that! Also don't try to use a blunt object to cut super thick mycelium. Just use a scalpel (duh!) Hopefully the Myco Gods were smiling upon me, and I can get a few decent transfer from all this mess. Goodnight everyone, and thank you for coming to my TED Talk. Edited by AndImTheHighOne (01/22/24 11:18 PM)
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SeventhMushroom Is this thing on?  Registered: 12/30/22 Posts: 60 |
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Reserving my spot for this year.
Just going to do only one spore sprint of golden teachers. Technically will be the first time I actually use a spore print instead of something else. Wish me luck Species: P. Cubensis Variety: Golden Teachers Day 0 Kicking off the new year! Started off getting everything ready, then realized.. I have no "swab" like utensil. Nor do I have any sterile water to mix the spores with. So, I'm just going to be using scraping the spores directly from the print, with my inoculation loop. This seems less than ideal, since it's solid metal, and likely wont grab many spores, but I mean, technically just one spore needs to come along for the ride, right? It's also a chance to test out my new flow hood I made!! More on that later, in another thread, but I built it to meet all the requirements for a flow hood from my 'research' on the topic. I used it to pour agar plates for the first time a week or so ago, and seems to have worked splendidly! I don't see any contamination on any of the plates. time will tell if this is repeatable  (my success rate with a SAB has not been great..)(plates are LMEA with 2% agar, and 0.1% LME iirc? maybe should have documented that better somewhere.. hm.) Here's one of the spore prints I'll be starting with :3  I started using the loop to scrape some spores off the print, then wipe it over the plates, but I wasn't sure I was really getting any spores. I guess time will tell. after a few plates, I tried *sanding* the surface of the loop to get more surface area for the spores. After that, I could visibly see spore clumps sticking to the loop, so I have better confidence on that. Overall process was simple. Flame the loop. Run the print. Wipe on the plate. Repeat. Total of 5 plates, using two golden teacher spore prints. (I also used some LC to inoculate some jars I had, unrelated to LAGM in the same sitting) And, my work is complete!  Day 11 First sign of life! 3 of the 6 plates have something fungus-like growing! I can't get a nice picture right now, sorry. And, it seems the 3 plates that have germinated are the 3 plates that I made after sanding my inoculation loop to get a more "grippy" surface texture to grab spores (since, well, no swabs were available). So, I guess maybe that worked? (picture taken on Day 20)  Day 17 I made a risky move today, and took some transfers directly to some LC vials. Either it works, or it won't. While I wouldn't advise others try it, I think it's going to be fine.. The plates themselves look rather clean, each one has a single germination spot of white fluffy mycellium. I'm pretty sure there's no mold at least. And, if there's bacteria, it will show up quickly in the vials in my experience. Call me lazy (: I didn't have any plates ready, but I some extra sterilized LC vials around...sooo... The plates have also been very slow to grow, I'm assuming because it's been pretty cold where they are. As for the transfer procedure, it was pretty straightforward: I use a pair of tweezers to remove the stopper from a vial under laminar flow, and place the stopper down upside-down. Take a tiny spec of myc from the plate with a flamed scalpel, and carefully wipe the myc off the blade into *the stopper*, the little well in the middle. It seemed easier than trying to shake it off directly into the vial, or scrape it off the side of the glass vial. The inside of the stopper should be as sterile as can be. Then, some tweezers to put the stopper back on. (need to find out how to rotate this...)  Day 20 My LC vials seems to be okay! I'm getting the nice, fuzzy growth I'm used to seeing, and I don't see any signs of cloudiness that I get from bacteria. Usually by day 3, the liquid gets noticeably cloudy from contamination in my (limited) experience. I also moved the vials from the cold closet, to a warmer space inside, and over the last 24 hours, there has been significantly more growth. Go figure. Here's a close-up of one of the vials, after swirling the contents around. You can see the little bit of agar, and the fuzzy "halo" around it as it's grown.  Edited by SeventhMushroom (01/20/24 03:22 PM)
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prim school cafeteria bully  Registered: 09/18/23 Posts: 47 Last seen: 2 years, 25 days |
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this is such a neat idea and i look forward to participating.
i try to grow something new every time and so to be determined. reserving my spot! Edited by prim (12/27/23 10:25 AM)
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ReverendMyc succinct is not my forte  Registered: 03/29/19 Posts: 3,465 Loc: uacious, but can't spel gud 
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Count me in please.
Let's go with:
I have several other things starting up now as well, but these are the ones I will do from spores on 2024-01-01 (the correct date format  ).2023-12-30 First update - Pre-Prep work. Making up some fresh agar plates. 16g LME, 18g Living Jin Agar Agar, 1000ml distilled water in pp pitcher in Instant Pot for 50 minutes after 10 minute vent. Pouring into 100mm Celtreat grip ring plates in front of my ffu. 2024-01-01 Put spores to grain after finishing the final season of Letterkenny and a Doctor Who episode. The original three prints and added three swabs of different lines of subtropicalis that arrived in time to play. I also have several other pan varieties on plates that I started before the first, so they are not going to be featured here.   I track everything with a serial number in sharpie and a spreadsheet for all notes.  2024-01-04 Got some action on Pan Bunnell and the Subtrop Bell Cap! Not all good. Bacterial brain blob.  But some good myc on both pan bunnell plates.   And stoked to see the subtrop bell cap already germing on one plate.  There might be some action on some of the rest, but too early to be certain.  2024-01-08 Transferred the most aggressive two plates to some new plates - Subtrop Bell Cap & Pan Bunnell Mexicana Chicon Nindo from print & Pan Cyan Bunnell from print both showing some signs of life Still waiting on the swabs to get started.  Also did some other things that I started before the 1st.  2024-01-10 Definitely seeing germ on Mex Chicon Nindo and Subtrop Hindsight. Hints of germ on the final two as well. 2024-01-12 Out of town for a while and missing them already. They grow up so fast. 2024-01-18 Home for a few days so checking in.  Here is the whole gang including both germs plates and the last 4 are t1s. Looks about time to get t1s from one of the Mexican Chicon Nindo and both of the Subtropicalis Hindsight. Still no action on the subtrop 0t0lerance and xica but I am patient.   The subtropicalis bell cap t1 plates already look really nice and may get a t2 and sent to grain soon. The pan bunnell are not as pretty but may get the same treatment because in my VERY limited experience with pans, sometimes the ugly plates out perform the pretty ones. 2024-01-19   t2 from the pan bunnells and t1 from one Mex Chicon Nindo and two Subtropicalis Hindsight. Going to do t2 transfers from the subtropicalis when I put them these plates to grain in a couple of days if the look worthy. t1 is usually too early for that, but I don't mind wasting a couple of jars on a chance. Also started some Golden Teachers from prints to plates and prints to swabs to pucks for another couple of projects. Its a grow off! Plates vs pucks. Used a print to do plates using a loop and two swabs dipped in sterile water and wiped on print to pucks. Lets see what happens.     2024-01-24 Home again so update time. Started prepping some grain for the subtropicalis bell cap t1s and 15 other non-lagm plates that are about ready. Grabbed update pix of transfer plates.  pan bunnell t1 and t2.  mexicana chicon nindo and subtropicalis t1s   golden teacher nothing to see yet Plan to get a t2 and put these t1 subtrop bell caps to grain tomorrow when the jars finish cooling.  2024-01-25 I actually executed the plan. Don't tell my wife that I can do that.   subtropicalis bell cap went to t2s and 2x1 pint millet grain jars. 2024-01-30 First shake of bell cap subtropicalis jars. Looking good so far. Forgot to snap a picture. So far nothing from the GT plates or pucks. And still nothing from from the subtropicalis xica or 0t0erance.   But it looks like time to put subtropicalis Hindsight, Mexicana Chicon Nindo, and pan bunnell to grain soon.  2024-02-01  Subtropicalis Hindsight. Sending these t1 transfers to grain after getting some t2s from them. Going to wait on the pans and mex that are moving slower. 2024-02-05  Going to grain and bunnells to t3 and mex to t2 tonight.  10 day old subtrop bell caps ready to spawn and 5 day old subtrop Hidnsight about to get first shake. And pf puck takes the early lead on the GT germination race!   No germination on the two agar plates, but one of the two pucks is taking off. I know this is an agar focused thread, but... EDIT: Correction, both pucks have germinated. Plates haven't yet. 2024-02-07 Spawned the subtrop bell cap in coir trays in tubs  Sent pan cyan bunnells to t3 and mexicana chicon nindo to t2 and both to grain.   First time using new 70mm glass petri dishes. I poured them too hot so there is a lot of condensation, but they were not too difficult to handle. Testing these out to see if I can make the switch from plastic plates. 2024-02-19 Back from 10 day trip with a progress report.  Subtropicalis Bell Caps about ready to case.  Subtropicalis Hindsight about ready to spawn.  Pan Cyan Bunnell about to shake and Mexicana Chicon Nindo also ready to spawn. 2024-02-21  Put CN to sub tray in tub with another cn started before LAGM  Cased subtrop bell caps  Put subtrop hs to trays in tubs as well. 2024-03-19 Been more than a minute since I updated LAGM. The Bell Cap Subtrops decided to come out and play.   Will do some inventory and figure out where the other LAGM projects are and do another update. Too many things going on at once to keep straight without a spreadsheet. 2024-06-17 Its been three months since my last Clones of the bell cap subtropicalis and I still have clones of the hindsight line of subtropicalis just put to a couple of trays. So I guess I am still in it.   Never got the Chicon Nindo to fruit but still have some culture running on agar plates. 2024-08-21 Been a while but these two subtropicalis grows are still going so I am sharing the tub logs of both. Subtropicalis Hindsight trays 1994 1995 from jars 1956 1957 2nd gen clone 1pt spawn 3pt compressed hpoo/coir 1/2 pt top layer Spawned 2024-06-14. Filter stickers on inside of all holes except 2 top center short side at spawn Day 3  Day 7   Lightly cased with a peat/verm/lime/sand casing I had on hand. Don't usually do sand for subtrops, but it was handy. Day 11  Day 19  Day 37    Pins Day 49  More pins and the first ones are fatties. No spores yet so I will leave them. Day 68 First harvest     Subtrop Bell Cap clone trays 1861 & 1862 from 1808 & 1809 2nd gen clone 1pt spawn 2qt highly compressed coir 1/2 pt top layer Spawned 2024-05-06 Only 2 top short side holes open on each side Day 0  Day 7   Before and after casing with pasteurized Peat/verm/gyp/lime Day 15  Day 23  Day 38  Day 42 Day 45   Day 50  Almost harvest time. Would like to get some prints. Day 52    Harvest but regrettably no time for prints this flush. Day 53  Day 58  Day 59  Day 64  Let them get a little too hot, but they are still going. Day 70  Opened two outer bottom holes on each short side. Probably too late of promote bell cap shape this flush, but they looked like they could use a bit more fae. Day 77  Ready to print I think. Day 88  Still going, but only because I don't need the space right now and I lazy. Day 107               
Edited by ReverendMyc (08/21/24 01:59 PM)
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Tiamo Trust in LITFA     Registered: 04/07/16 Posts: 1,936 Loc: Amsterdam 
Last seen: 2 years, 1 month |
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Reverend, I always knew you were a man of culture. ISO 8601 for the win.
I want to do a Ps. natalensis en Pan. cyan grow for LAGM 2024. Starting from my own prints, which I have yet to grab since I am working witb other people's prints now. Taking it from spores to clone and testing the potency of the dried fruits along the way. Let's have some fun.
-------------------- 
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just 
 Mush love
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cyb3rtr0n searching for truth   Registered: 05/06/09 Posts: 1,832 |
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For LAGM 2.024, I'll try various species/varieties in this same fruiting chamber.
Currently in FC: Panaeolus cyanescens TTBVI plan to attempt/grow: - various Panaeolus cyanescens - Psilocybe caerulescens - Zapatacorium - possibly others Update 3/16 I started some plates in early January, then made some cakes and an LC of TTBVI> This year, I plan to try a few exotics, starting with pan.cyanescens TTBVI. I've been busy, so I haven't taken many pictures, and some stages of growth to spawn were late to transfer along the way. TTBVI:  5 Days later on Shoebox, 4 days later on cakes (First Flushes)   Edited by cyb3rtr0n (03/22/24 08:55 AM)
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Dirtbaggery  Registered: 09/20/23 Posts: 71 Loc: LIGMA Last seen: 1 year, 5 months |
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might as well give it try! Right now the only thing I have on swab/print is Thai Lipa but lets run it!
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stubb Dahg Rastubfari  Registered: 03/23/19 Posts: 1,661 Loc: Memory 
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 Team 8601!
Edited by stubb (02/02/25 04:13 PM)
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SeventhMushroom Is this thing on? Registered: 12/30/22 Posts: 60 |
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🆃🄴🅰🄼 🅸🅂🅾⑧⑥🄋①
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thirdeyewild  Registered: 11/01/13 Posts: 1,718 Loc: 
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Nat3: Tidal wave Nat hybrid back crossed with Jack Frost
I think I'll join this time I have a nat x tw hybrid that I back crossed with jack frost. I'm going to grow out the f2's if I can find the swabs. Jan 2nd update. Found the swabs, there were two , looked pretty light. I'll give them a week, if no growth I'll dig up another interesting cross. Maybe brainfreeze... Jan 14: germination! Going to cook up some agar tonight for the transfer. March 12: Spawning 400ml spawn to 75 grams coir hydrated with 375ml water.  March 29: Cased a few days ago. Think I'm going to call these Nats3 (Nats cubed) April 21: 1st pin! 😁 41 days from S2B, I was starting to get worried. Looking like a Lucy.  April 27: This one was dropping spores, so I harvested it to print. These are actually F1 not f2, so I'm going to run the again and look for neat phenos. I loved the forked gills and wonky nip! There are several more coming in now and I have another mini bag about ready to fruit too      May 27, I was hoping one of these would show up.  August 8 So I ran spores from 3 phenotypes. The super nippley, the albino and the large. The albino and large are going to be s2b in a few days. The nips have just started pinning. The last batch took 5 weeks to pin I think. These are about 7 days from casing. F2 of (nat x tw) x jf. First 2 pins are albino!  
Edited by thirdeyewild (08/10/24 03:32 PM)
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EniQma PȘÝČĦËĎËŁĪČ  Registered: 11/28/23 Posts: 2,456 |
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What’s the objective? First canopy?
-------------------- Thank your postal workers when you see them. None of this would be possible without them. Necessity is the mother of invention
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WyoMX  Registered: 07/06/15 Posts: 2,356 Loc: PNW |
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Still not in a position to grow right now being in a shared house and all that jazz but love following the lagm threads so I'm excited to see what all you fine ladies and gentlemen and whoever else can get done this year!
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bigfootscreepyuncl Stranger Registered: 11/15/20 Posts: 3,736 Loc: Gamehenge |
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Not really any specific objectives necessarily..just a bunch of people growing fungus together, documenting their progress along the way! In the past there have been prizes for first fruit, first canopy, biggest blah blah blah but that is 100% up to anyone who wants to put up a prize. LAGM got started some time ago in order to show beginners that agar isn't scary or very difficult. The idea was that there would be a community of people who understood agar that would help beginners out as they needed it to move on from shooting spores into grain jars. Very noble origins indeed! Now it's mostly a dick measuring contest amongst assholes (who are still very willing to help beginners if they need it  )Go back to page 1 and you can see my example of how I started my grow log. Your grow log will be the post I'm responding to but you continuously update/edit it throughout the year, or however long it takes you to accomplish your goal. So hurry up and join it's super fun!! -------------------- 5318008
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EniQma PȘÝČĦËĎËŁĪČ Registered: 11/28/23 Posts: 2,456 |
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How do I join and is agar a requirement? For finding clone material my go to procedure is dropping spores from a print to a small amount of oats in a quart jar then adding a top layer of coir once colonized. I do use agar but not for speed and leisure
-------------------- Thank your postal workers when you see them. None of this would be possible without them. Necessity is the mother of invention Edited by EniQma (12/27/23 07:56 PM)
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WyoMX Registered: 07/06/15 Posts: 2,356 Loc: PNW |
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Yeee spores to agar is how everyone should start.
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[Re: 
Edit 
Reply 
like. Here be some nats:
| Shop: Spores for European Microscopy APE Liquid Culture For Sale Bulk Cannabis Seeds All-In-One Bags That Don't Suck Liquid Cultures Unfolding Nature: Being in the Implicate Order Bulk Substrate |
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