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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: EniQma] 3
#28609238 - 01/04/24 07:54 AM (24 days, 9 hours ago) |
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Well just as anyone would have guessed there is no action on any of my germ plates which were inoculated less than 12 hours ago the compulsive observations of my projects have officially begun!
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I 5318008 NOT a virgin!
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MojaveMyc
Fractal Farmer


Registered: 05/08/21
Posts: 656
Loc: Colorado
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Hahahah same energy here. I’m finding it fun to be back in that mindset. Fingers crossed you see some action soon 🤞🏾
OP updated
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 1 hour, 31 minutes
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Quote:
bigfootscreepyuncl said: Well just as anyone would have guessed there is no action on any of my germ plates which were inoculated less than 12 hours ago the compulsive observations of my projects have officially begun!
Pics or it didn’t didn’t happen.
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 2 hours, 43 minutes
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-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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OP updated with the following: Got some action on Pan Bunnell and the Subtrop Bell Cap! Not all good. Bacterial brain blob.

But some good myc on both pan bunnell plates.
 
And stoked to see the subtrop bell cap already germing on one plate.

There might be some action on some of the rest, but too early to be certain.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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This is serving as my grow log for the entry:
I am starting to see growth on my swabbed plates.
I have APE-R, PE6, and Pan Cyan BVI



Update 1/14
I forgot to take pictures, but I made my first transfers to home made agar.
My Pan Cyan mycelium turned yellow, so definitely dealing with some kind of bacterial infection. I took transfers from the outside edge as small as possible. I'm going to let those grow for a week and see how they look. I ordered additional Pan Cyan spores just in case I can't clean these up.
My PE6 and APE-R both looked nice. I made 4 transfers each from 5 plates.

Update 1/21
I usually shower immediately before doing my SAB work, but had a busy day so I only showered in the morning. It definitely shows in my agar work.
My clean procedure is this: -Shower -Brush Teeth and use mouth wash -Put on clean clothes -Wash hands with soap and water -Put on Gloves and Mask -Wipe inside of SAB with soap and water to clean -Wet sides of SAB with soapy water -Spray inside with lysol spray -Let sit for 2 minutes -Sanitize gloves with 70% Iso
APE-R did the worst, I didn't end up with a single sample that is 100% clean. There is still enough healthy mycelium to make another round of transfers. I'm going to take from the nicest growing ones and discard the rest.

PE6 did OK, some of the plates were extremely bacterial, but I did end up with one 100% clean plate. I plan to make a LC from that plate, and make multiple transfers to expand my culture collection.

Pan Cyan is the one I have the highest hopes for. All the cultures look really good with very little bacterial contamination. I'm going to make a LC using the whispyiest one, and transfer the rest to multiple plates.

All in all, I've learned that my clean procedure needs work. If anyone has any recommendations I'm all ears.
Edited by CaptainPuffy (01/21/24 07:57 PM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
CaptainPuffy said: Can I get in on this? I just put spores to agar a few days ago
I have APE-R, PE6, and I'm trying Pan Cyan
I'm also trying my hand at liberty caps and what lovers but I don't have the spores yet
Hell yeah you can get in on this action!
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I 5318008 NOT a virgin!
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Vibetyme
Smoke 1



Registered: 06/08/21
Posts: 1,763
Loc: Florida
Last seen: 50 seconds
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Alright I decided to go with Albino Riptide, Starry Night Ape and Toque.
Mostly because they all they look cool as hell.
I only have one swab of each, so some luck might be required.
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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Good luck mate
-------------------- LAGM 2024
Thank yoU please come again FrienD
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AndImTheHighOne
Stranger


Registered: 12/06/23
Posts: 44
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Re: LAGM v. 2.024 [Re: mnj] 1
#28609826 - 01/04/24 07:04 PM (23 days, 22 hours ago) |
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Updated my post. Seeing some type of growth. I think it's good but I honestly don't have a clue yet lol
-------------------- "[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate " ~ Bigfoot My LAGM 2.024
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: Vibetyme] 1
#28609836 - 01/04/24 07:13 PM (23 days, 22 hours ago) |
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Quote:
Vibetyme said: Alright I decided to go with Albino Riptide, Starry Night Ape and Toque.
Mostly because they all they look cool as hell.
I only have one swab of each, so some luck might be required.
Starry Night you say? I wish you all the best luck Vibe! Because once you get a flush I'm gonna hit you with that 
I'm not sure but I might have an albino riptide swab I'd be willing to share with you if yours doesn't work out..either that or an albino tidal wave. IDK it's been a long time since I've gone through my cube spores lol
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I 5318008 NOT a virgin!
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Vibetyme
Smoke 1



Registered: 06/08/21
Posts: 1,763
Loc: Florida
Last seen: 50 seconds
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It's a SN swab and while I hope for the best I honestly think it's gonna be hard getting more then one or two actual SN fruits coming from spore.
my apes have always been shit but I keep trying.
If I do get something cool you already know I'll definitely share with you homie. I'll hit you up if need be on that swab. Thanks for the offer!
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Re: LAGM v. 2.024 [Re: Vibetyme]
#28609906 - 01/04/24 08:19 PM (23 days, 21 hours ago) |
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I just heard about this... Too late to throw my hat in?
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Nope! There will be people joining over the next month or two. That's the beauty of LAGM, you can't be too late, only too early lol
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I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said: Nope! There will be people joining over the next month or two. That's the beauty of LAGM, you can't be too late, only too early lol
Okay. I was hoping to obtain some better genetics, but I DID inoculate 4 dishes on 1/1.
x2 JMF, MSS syringe (syringe is sus, I'm honestly not hopeful) x2 Enigma, LC syringe (not from spores so maybe doesn't qualify?) (hopefully one or two more to come)
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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stockw

Registered: 08/31/21
Posts: 450
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Let's gooooooo
First time growing both of these so let's see how it goes 1. Ps. Cubensis - Na Mueng 2. Pan. Cyanescens - FL Red Spore F3
Method: Spore -> Agar -> LC -> Grain -> Bulk
Edited by stockw (01/07/24 06:22 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: stockw] 2
#28610334 - 01/05/24 10:48 AM (23 days, 7 hours ago) |
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Still nothing to report on....Maybe if I check on my plates more frequently they'll germinate faster!
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I 5318008 NOT a virgin!
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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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I know that you are joking, but it really works. You probably just aren't checking often enough.  Try blinking less.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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edixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 12 hours, 23 minutes
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Imma have to go check my plates again
-------------------- ~ LAGM 2024 ~
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
ReverendMyc said:Try blinking less.
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I 5318008 NOT a virgin!
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