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Tiamo
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Miraculix Psilocybin QTest Results 5
#28554722 - 11/24/23 07:39 AM (2 months, 2 days ago) |
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Miraculix is a German company that has developed a rapid quantitative psilocybin equivalent test kit for at home use. At the time of writing a single application can be purchased starting at €12,95 in the EU or $18 in the US. Miraculix claims their quantitative psilocybin tests deviate at most 10% from laboratory analysis using the HPLC technique. Now, this is not an advertisement for these quantitative tests per se, but I do think it is a good thing that relatively inexpensive quantitative psilocybin test kits are available on the market and I think we could all benefit from sharing the results of these tests with each other for harm reduction purposes and to possibly identify factors that influence the potency of cultivated magic mushrooms.
Today, I used one of these tests to test a recent harvest of Psilocybe cubensis Penis Envy (PE). This culture of PE was acquired from a commercial vendor in The Netherlands, purchased as a liquid culture (LC) and grown out on a substrate of coco coir and vermiculite, using a roughly 1:4 spawn ratio, using a p9 style top layer, in an unmodified 45 liter monotub at a temperature of roughly 19 degrees Celcius or 66 Fahrenheit. The time from spawning to harvest was approximately 5 weeks.
A sample of 5 random mushrooms weighing around 4 grams was taken, ground up in a mortar and pestle and tested. The results of this test were as follows:

Now, although the image makes it look like the sample was tested at 24 mg psilocybin equivalent per gram, in real life the color actually appeared much closer to 21 mg psilocybin equivalent per gram.
I call to action any Shroomerites who have used this test kit or are planning to use this test kit to share their results and any other factors they would find relevant to add data points to the alkaloid contents of cultivated magic mushrooms. Thank you!
Shroomery's Miraculix Psilocybin QTest Results

Definitions
- Mono = monotub
- Shoe = shoebox
- CV = coco coir, vermiculite
- PC = pseudocasing
- OTS = off the scale
More interesting experiments with the Psilocybin QTest:
Edited by Tiamo (12/30/23 02:58 PM)
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cidero
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 2
#28554786 - 11/24/23 09:09 AM (2 months, 2 days ago) |
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Hope many people share their results. Would be great to have a table with all the results in the first post
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JW123
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 4
#28554818 - 11/24/23 09:50 AM (2 months, 2 days ago) |
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ReverendMyc has been working with them alot. https://www.shroomery.org/forums/showflat.php/Number/28478167#28478167 Hopefully they drop in here.
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Tiamo
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Re: Miraculix Psilocybin QTest Results [Re: JW123]
#28555042 - 11/24/23 01:52 PM (2 months, 2 days ago) |
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Quote:
JW123 said: ReverendMyc has been working with them alot. https://www.shroomery.org/forums/showflat.php/Number/28478167#28478167 Hopefully they drop in here.
I have been following that thread. They are looking at the potency of dried grain spawn for Psilocybe subtropicalis. Not exactly a very standard scenario. Maybe they have also tested other varieties, I'd like to hear it.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Kinoko314
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 2
#28555135 - 11/24/23 03:25 PM (2 months, 2 days ago) |
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I think the overall amount of testing has been pretty low. Not a lot of people want to spend that much on testing when that money could go to other mycology stuff.
With the link in your sig, maybe we can slowly get people to post their data if they have it. Now you just have to post everywhere always and we'll be all set!
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JW123
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Re: Miraculix Psilocybin QTest Results [Re: Kinoko314] 1
#28555363 - 11/24/23 06:41 PM (2 months, 2 days ago) |
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Tiamo
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Re: Miraculix Psilocybin QTest Results [Re: JW123]
#28555780 - 11/25/23 08:18 AM (2 months, 1 day ago) |
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Quote:
JW123 said: Subtropicalis fruits from Rev https://www.shroomery.org/forums/showflat.php/Number/28517696#28517696
Yea, those are definitely way above 24 mg/g. 20 mg is on the upper end for cubensis and a lot of exotics are considered up to five times as strong, such as Panaeolus species.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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ReverendMyc

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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 6
#28558894 - 11/27/23 09:22 PM (1 month, 29 days ago) |
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Nice thread, Tiamo. Cross posting new results that I just got for Pan BVI (British Virgin Island).
I expected the pans to be too potent for the test chart so I used half the amount per instructions from Miraculix (the maker of the potency test kits). So using 0.075 grams instead of 0.150 grams means you double the reading on the test.
   
I am reading 21mg/g x 2 for 4.2% actives. Tallest reading for me to date.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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Tiamo
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Re: Miraculix Psilocybin QTest Results [Re: ReverendMyc] 2
#28559013 - 11/28/23 02:01 AM (1 month, 29 days ago) |
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42 mg/g is an insane result. That's about 4 times as potent as your average cube. Wow! 
Might want to use a fourth of 150 mg for Panaeolus and other potent exotics in the future to have some margin.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
Edited by Tiamo (11/28/23 03:15 AM)
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LogicaL Chaos
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 1
#28559029 - 11/28/23 02:33 AM (1 month, 29 days ago) |
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This is so cool! 
Thanks for sharing!
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Muffa
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Re: Miraculix Psilocybin QTest Results [Re: LogicaL Chaos] 4
#28567973 - 12/04/23 08:49 AM (1 month, 23 days ago) |
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Here is my test for APE:
 This was my first test ever and I didn't belive it, even with the 10% error range the result felt too high so I decided to give it to four people to test it and I also tested it myself. With 0.5g I had a really strong trip but lasted only 3 hours with some visuals but nothing extreme, with 1g it lasted at least 5 hours and my face melted with everything around me, others reported similar experiences.
I will be testing every batch I make every 100-150g, it doesn't feel right to me to give others something that I don't know the potency of.
The procedure I follow is to powder the 100g and mix it to even the distribution of active compounds, then take a sample using a high precision scale and test it.
Edited by Muffa (12/04/23 08:50 AM)
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Tiamo
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Re: Miraculix Psilocybin QTest Results [Re: Muffa] 1
#28567987 - 12/04/23 09:02 AM (1 month, 23 days ago) |
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I can believe it after seeing my PE results. I tried microdosing at 200 mg and got some visuals in the tiles when going to the bathroom and felt generally inebriated. That wasn't a microdose. 
Thanks for sharing!
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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elasticaltiger
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 2
#28568094 - 12/04/23 10:32 AM (1 month, 23 days ago) |
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Quote:
Today, I used one of these tests to test a recent harvest of Psilocybe cubensis Penis Envy (PE). This culture of PE was acquired from a commercial vendor in The Netherlands and grown out on a substrate of coco coir and vermiculite, using a roughly 1:4 spawn ratio using a p9 style top layer in an unmodified 45 liter monotub at a temperature of roughly 19 degrees Celcius or 66 Fahrenheit. The time from spawning to harvest was approximately 5 weeks.
I wish every n00b's posts were this succinct when they post about their grows.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Zwinst
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Here are some older results from my testing:

Cubes seem to fall in the same range with little deviation. And i think the cause for mine beeing so strong has more to do with my substrate than genetics. Except for the mutants, if they take longer to mature their spores they get stronger.
Of course i plan on doing more in the future, but i have something like 20 different species and about 40 strains i could test...
I also tested allenii from my garden this fall and they went above 2,4%. Duh. But i am not going to retest them with only 75mg until i have samples of all the subaeruginosa subspecies in my collection. I want to know if azurescens really is stronger than cyanescens for example. And i kind of want to know if my subaeruginosa strain from New Zealand is as weak as the ones i ate in Australia. They were kind of disappointing...
Edited by Zwinst (12/17/23 04:25 PM)
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Tiamo
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Re: Miraculix Psilocybin QTest Results [Re: Zwinst]
#28573816 - 12/08/23 03:07 AM (1 month, 19 days ago) |
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Quote:
Zwinst said: Here are some older results from my testing:

Cubes seem to fall in the same range with little deviation. And i think the cause for mine beeing so strong has more to do with my substrate than genetics. Except for the mutants, if they take longer to mature their spores they get stronger.
Of course i plan on doing more in the future, but i have something like 20 different species and about 40 strains i could test...
I also tested allenii from my garden this fall and they went above 2,4%. Duh. But i am not going to retest them with only 75mg until i have samples of all the subaeruginosa subspecies in my collection. I want to know if azurescens really is stronger than cyanescens for example. And i kind of want to know if my subaeruginosa strain from New Zealand is as weak as the ones i ate in Australia. They were kind of disappointing...
Thanks for sharing! That's a lot of data. 
Why do you believe your substrate affects the potency of your mushrooms?
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Zwinst
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 2
#28573820 - 12/08/23 03:20 AM (1 month, 19 days ago) |
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Quote:
Tiamo said:
Why do you believe your substrate affects the potency of your mushrooms?
I started using only wheat as substrate and my cubes weren´t really that strong and i have seen lots of people doing the same over the years, using only one type of grain and they typically also got weak cubes. PF tek is also a good example, rice sucks ass for potency. For a while now i am using a mix of 2 brands of birdfeed that contain probably 15 or more different types of grain including hemp. And now i get pretty strong cubes. Using fertilised coir and letting the fruiting bodies mature as long as possible may play a role too. I was surprised by my results, to be honest. I knew that my GT strain is pretty good, but i never imagined the others where too. The only real outlier was the John Allan strain i rescued from an ancient print, but still it did really good with 1,6%.
Edited by Zwinst (12/08/23 03:22 AM)
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Tiamo
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Re: Miraculix Psilocybin QTest Results [Re: Zwinst] 1
#28573828 - 12/08/23 03:29 AM (1 month, 19 days ago) |
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I am really sceptical that the grain used could affect the potency. Can you point out the tests that used wheat as a grain vs. other grain?
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Zwinst
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Re: Miraculix Psilocybin QTest Results [Re: Tiamo] 2
#28573832 - 12/08/23 03:41 AM (1 month, 19 days ago) |
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There were no q-tests yet when i was still using mono-grain. Thats like 10 years ago.
But it would be a nice test to do.
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LogicaL Chaos
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Re: Miraculix Psilocybin QTest Results [Re: Zwinst]
#28573871 - 12/08/23 05:25 AM (1 month, 19 days ago) |
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Maybe do a single grain wheat grow, for old times sake
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pucktheduck
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Re: Miraculix Psilocybin QTest Results [Re: Zwinst] 1
#28580589 - 12/12/23 09:48 PM (1 month, 14 days ago) |
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Quote:
Zwinst said:
Quote:
Tiamo said:
Why do you believe your substrate affects the potency of your mushrooms?
I started using only wheat as substrate and my cubes weren´t really that strong and i have seen lots of people doing the same over the years, using only one type of grain and they typically also got weak cubes. PF tek is also a good example, rice sucks ass for potency. For a while now i am using a mix of 2 brands of birdfeed that contain probably 15 or more different types of grain including hemp. And now i get pretty strong cubes. Using fertilised coir and letting the fruiting bodies mature as long as possible may play a role too. I was surprised by my results, to be honest. I knew that my GT strain is pretty good, but i never imagined the others where too. The only real outlier was the John Allan strain i rescued from an ancient print, but still it did really good with 1,6%.
When I first started growing in like 07 I used only rye and that worked out ok.
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