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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: EniQma]
#28626045 - 01/18/24 03:15 PM (10 days, 2 hours ago) |
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Quote:
EniQma said:Haha bro I knew you were speedin your tits off with all tho posting you were doing! I was also wondering how the hell you lucked out with such fast colonizers.
Don’t beat yourself down. Shit happens
Well, several years ago JCM went from agar to harvest in like 12 days and I'm working with one of those prints so I just thought I got lucky with a lightning quick culture (and I was expecting this speed to be linear across all varieties, which is not the case). His thread is the one I studied the most, especially the culture selection section.
What I think actually happened is I have been using yeast plates lately for my cubes which provides a thicker, more robust growth habit for the mycelium, and the yeast caused the otherwise thin and wispy growth (desired for this species) to express itself in a much more tomentose form (not desired for this species) so I avoided transferring from the growth I should have been targeting. Meanwhile, mold mycelium didn't give a single fuck about the yeast in the agar and expressed itself as it naturally does, thin and wispy cultures of mycelium, which is what I was looking for! So, I believe I used an excessively dense nutrient recipe for the species causing a case of mistaken identity. I hit the trifecta for growing mold accidentally on purpose lol.
Live and learn. I need to pour some plates anyway so I'm going to pour a couple sleeves of some 'weak' plates for pans, omitting yeast and pour a sleeve or two of my regular cube plates.
Also, since I'm starting over with my entire LAGM project, I think I'm going to take this opportunity and do the entire process from pouring plates to inoculating jars in front of my shitty flow-hood. This will be a good test to see if it's actually usable. I know it's turbulent so far from ideal but if it's usable I may as well use the damn thing!
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I 5318008 NOT a virgin!
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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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Re: LAGM v. 2.024 [Re: Noncense]
#28626189 - 01/18/24 04:59 PM (10 days, 54 minutes ago) |
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2024-01-18 Home for a few days so checking in.
 Here is the whole gang including both germs plates and the last 4 are t1s. Looks about time to get t1s from one of the Mexican Chicon Nindo and both of the Subtropicalis Hindsight. Still no action on the subtrop 0t0lerance and xica but I am patient.
  The subtropicalis bell cap t1 plates already look really nice and may get a t2 and sent to grain soon. The pan bunnell are not as pretty but may get the same treatment because in my VERY limited experience with pans, sometimes the ugly plates out perform the pretty ones.
OP updated
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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In solidarity with bigfootscreepyuncl I'll turn LAGM into Cultivation Confessional and add my minor update:
My original AA+ plate had only germinated in one spot as a bright white fuzzy mass that reached as high as it did wide. Took transfers to a few plates anyway to see what was up (because the Oak Ridge I did last year also started out growing very vertical on the germ plates but performed super well!). Anyway, the two T1 plates are growing fast and fuzzy and I don't trust em. Today I put em in a ziploc and then a Tupperware and set em aside to see what happens. Either I've got two dope mold plates or I'll have plins in mid February. My gut says mold.
Also I ditched my PE because it was a translucent top crawler and overall the agar it touched looked gross. Idk wtf it was but it wasn't worth my time.
I've already noc'd up a few fresh AA+ plates bc I've never grown this variety and wanna give it a go.
The PE I'm ditching for LAGM bc I already have a few clone cultures going. Might do some top fruited cakes if I want more options but that won't be part of this thread.
Keep at it creepyuncl, I know how excited you are to run exotics
-------------------- LAGM 2024
Thank yoU please come again FrienD
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: mnj]
#28626298 - 01/18/24 06:07 PM (9 days, 23 hours ago) |
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Quote:
mnj said: In solidarity with bigfootscreepyuncl I'll turn LAGM into Cultivation Confessional and add my minor update:
My original AA+ plate had only germinated in one spot as a bright white fuzzy mass that reached as high as it did wide. Took transfers to a few plates anyway to see what was up (because the Oak Ridge I did last year also started out growing very vertical on the germ plates but performed super well!). Anyway, the two T1 plates are growing fast and fuzzy and I don't trust em. Today I put em in a ziploc and then a Tupperware and set em aside to see what happens. Either I've got two dope mold plates or I'll have plins in mid February. My gut says mold.
Also I ditched my PE because it was a translucent top crawler and overall the agar it touched looked gross. Idk wtf it was but it wasn't worth my time.
I've already noc'd up a few fresh AA+ plates bc I've never grown this variety and wanna give it a go.
The PE I'm ditching for LAGM bc I already have a few clone cultures going. Might do some top fruited cakes if I want more options but that won't be part of this thread.
Keep at it creepyuncl, I know how excited you are to run exotics 
Oh yeah, Pans got me down but not out this heavyweight match is going all 12 rounds boi!
It sucks you're experiencing difficulties but this is literally what LAGM was created for. Help beginners learn agar and show them that mistakes are inevitable and everyone, regardless of experience strikes out every now and then. Keep your stick on the ice mnj 
AA+ is a fun variety, not too picky about conditions and readily throws canopies. Be sure to harvest before spore drop because they drop a LOT of spores ALL at once. If your new AA+ plates go tits up hit me up I've got some clean prints I'd be happy to share and a bangin' PE culture to boot.
So far everyone except me and mnj are doing pretty good keep up the good work everyone!
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I 5318008 NOT a virgin!
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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Yah man we got this
Just checked the AA+ and Pan Cambo Thai plates I noc'd up on Sunday and I've got itty bitty germination happening 
Updates to follow within the next week
Edit: I'm gonna run with what I have but many thanks for the offer on prints. Solid.
-------------------- LAGM 2024
Thank yoU please come again FrienD
Edited by mnj (01/18/24 06:26 PM)
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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oxo
oxo

Registered: 11/27/22
Posts: 107
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Quote:
mycosispeon said: OP updated. T1s taken!
What are the little blips on the T1 plates?
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MojaveMyc
Fractal Farmer


Registered: 05/08/21
Posts: 656
Loc: Colorado
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Re: LAGM v. 2.024 [Re: oxo] 1
#28627616 - 01/19/24 06:31 PM (8 days, 23 hours ago) |
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OP Updated.

The pans are on grain. Gandalf needs a little love & I’m still unsure about the pollock plate. If it turns out to be mold I’ll be pulling out those lovely bell cap semps
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Re: LAGM v. 2.024 [Re: oxo]
#28627712 - 01/19/24 08:02 PM (8 days, 21 hours ago) |
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Quote:
oxo said:
Quote:
mycosispeon said: OP updated. T1s taken!
What are the little blips on the T1 plates?
Condensation. I've had the dishes stored upside-down and when I went to use them, I let as much of it drip out without fucking with it too much. I've had them stored upside-down since and now all the remaining condensation has be re-absorbed.
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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oxo
oxo

Registered: 11/27/22
Posts: 107
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Quote:
mycosispeon said:
Quote:
oxo said:
Quote:
mycosispeon said: OP updated. T1s taken!
What are the little blips on the T1 plates?
Condensation. I've had the dishes stored upside-down and when I went to use them, I let as much of it drip out without fucking with it too much. I've had them stored upside-down since and now all the remaining condensation has be re-absorbed.
Interesting, thanks.
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SeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 2 days, 8 hours
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Quote:
bigfootscreepyuncl said: Also, since I'm starting over with my entire LAGM project, I think I'm going to take this opportunity and do the entire process from pouring plates to inoculating jars in front of my shitty flow-hood. This will be a good test to see if it's actually usable. I know it's turbulent so far from ideal but if it's usable I may as well use the damn thing!
What's so shitty about your flow-hood?? And how do you know it's turbulent?
-------------------- LAGM 2024
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
SeventhMushroom said:
Quote:
bigfootscreepyuncl said: Also, since I'm starting over with my entire LAGM project, I think I'm going to take this opportunity and do the entire process from pouring plates to inoculating jars in front of my shitty flow-hood. This will be a good test to see if it's actually usable. I know it's turbulent so far from ideal but if it's usable I may as well use the damn thing!
What's so shitty about your flow-hood?? And how do you know it's turbulent?
Well, a friend of mine has a mushroom farm and he was upgrading so I traded a friend of mine a few ounces of PE for it. He took shit care of it once he got in his 3 new meyers 2x4's. All of the aluminum fins on the filter are bent and mangled. One quadrant of the filter has holes in it deep in the pleats so I can't find them to seal them, but I know they're there because every time I test the filter with plates that quadrant contaminates every single time. But, the other 3 quadrants don't.
I know it's turbulent because I took the blower out thinking I'd just get a new filter and build a new, better one. After running the numbers dozens of times the math just doesn't line up at all. The motor is WAY too powerful for the 2x4 filter I have. To have true laminar flow I'd probably need like a 3x6 or a 2x8 filter for the blower motor I have. It'll blow out a lighter from like 2 feet away lol.
BUT, I have been playing with it recently. I've done some agar transfers in front of it and they haven't contaminated yet and I inoculated 4 jars of grain and they're looking great. So, despite being able to use only 75% of the filter and knowing that it's turbulent I think it will still be usable for the little bit of work I'll do in front of it.
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I 5318008 NOT a virgin!
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SeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 2 days, 8 hours
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A hack you might try, if the blower is too powerful, rather than replace anything, just drill/poke a (small) hole in the side of the box to relieve the pressure before the filter. Make the hole larger until it is no longer turbulent. Wasted energy? sure, but if you can bleed off enough of the pressure, you can get the flow rate down to a more acceptable level. From my research, there should be a large acceptable range from ~40FPM - ~120FPM velocity, and lower is probably better. And hey, if it doesn't work you can always tape over the holes again. Just my 2 cents.
-------------------- LAGM 2024
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SeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 2 days, 8 hours
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On another note, OP updated with the status of my LC. Looks like it's doing okay so far!
-------------------- LAGM 2024
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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That's a happy update SeventhMushroom 
-------------------- LAGM 2024
Thank yoU please come again FrienD
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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Re: LAGM v. 2.024 [Re: mnj]
#28630389 - 01/21/24 08:03 PM (6 days, 21 hours ago) |
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OP updated
Ended up with a couple decent transfers and a lot of plates showing bacteria. Still salvageable.
I know the consensus of the community is to use soapy water, but I seem to be having a higher rate of bacterial contamination from just soapy water instead of 70% Iso.
I have to be extremely strict with my clean procedure with soapy water. I believe my point of failure this time was that I showered in the morning and made my transfers in the evening. In the past when I used 70% iso I didn't have to be so strict. Just wash my arms really well and make sure I showered at some point that day.
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
CaptainPuffy said: I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.
Scrub my arms like I'm prepping for surgery, gloves, hat, covid mask. Iso forearms and gloved hands. Never spray any liquids on the floor of my SAB and I don't use one of those cooling sheets, I just use blank plates as a 'false bottom' to keep everything elevated off the SAB floor. I shower every day but it rarely aligns itself with my SAB session and I pay no attention to it.
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I 5318008 NOT a virgin!
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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Quote:
bigfootscreepyuncl said:
Quote:
CaptainPuffy said: I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.
Scrub my arms like I'm prepping for surgery, gloves, hat, covid mask. Iso forearms and gloved hands. Never spray any liquids on the floor of my SAB and I don't use one of those cooling sheets, I just use blank plates as a 'false bottom' to keep everything elevated off the SAB floor. I shower every day but it rarely aligns itself with my SAB session and I pay no attention to it.
Thanks for the explanation! I definitely scrub/ISO my arms and wear gloves and a mask. I'll try running a dry SAB next time and see what happens
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Vibetyme
Smoke 1



Registered: 06/08/21
Posts: 1,763
Loc: Florida
Last seen: 55 seconds
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For all my shoebox growers out there who have had issues with fungus gnats. I think I came across a permanent solution.
The wife loves her indoor plants and we had a fungus gnat problem during the summer. I tried various things to get rid of them, like the vinegar & dish soap traps, a fan/uv light/sticky trap combo called the katchy, and various other sticky traps. They all kinda worked but didn't really take care of the issue 100%.
I started thinking about a bag to put my tubs in that would let light and air in but keep fungus gnats the hell out. I got on walmart.com and put my plan into motion. I ordered some of these fine mesh laundry bags.


The holes are so small the gnats can't get in.
The ones I ordered are a little bigger then my shoeboxes, but you could probably find some bigger ones if you search. At $2.97 per bag they are a hell of a deal if you have ever dealt with those little fucks.
I'm also gonna make a post about this in the main mushroom cultivation thread cuz the people need to know about this.
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Vibetyme
Smoke 1



Registered: 06/08/21
Posts: 1,763
Loc: Florida
Last seen: 55 seconds
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Re: LAGM v. 2.024 [Re: Vibetyme] 1
#28630589 - 01/22/24 01:17 AM (6 days, 16 hours ago) |
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We have lift off on all them thangs! Super stoked about these varieties! Toque ,Albino Riptide, and Starry Night Ape
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