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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,499
Loc: USA
Last seen: 40 minutes, 55 seconds
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Ahhh well if they’re not a need, then fuck em 🤣
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Silentraindrops
mushlove student

Registered: 12/23/23
Posts: 222
Loc: pnw
Last seen: 17 hours, 50 minutes
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Quote:
bigfootscreepyuncl said:
Quote:
Silentraindrops said:Idk if my prints are sterile but its fun lol
In this hobby there is a huge difference between clean and sterile and it's critical to know the difference.
I know I know , I'll change to very clean. Also the ones i grab for prints are grown in a "sealed chamber", I 100% know that I'm only lowering the odds but it's nice to think I'm giving them a good chance. I've also been goofing off with some open air experiments with my agar. When I go looking for contams i cant get them, But when I .... bam.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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You could have cleaned those up in a number of transfers using a few little tricks, but yeah, your other plates are much better. Good call it's easy enough for work to pile up in this hobby so if you don't need to save a culture, then fuck it
--------------------
I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said: You could have cleaned those up in a number of transfers using a few little tricks, but yeah, your other plates are much better. Good call it's easy enough for work to pile up in this hobby so if you don't need to save a culture, then fuck it 
Yeah I definitely have a good amount of irons in the fire
- AA+ tub goin (albeit badly)
- KSSS tub goin (albeit badly)
- inoculated 4 grain jars with JMF last night
- inoculated 4 grain jars with Mazatapec last night
- have 2 plates of Enigma rockin
- have several spore samples in the mail, of which I'll be selecting two for LAGM
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AndImTheHighOne
Stranger


Registered: 12/06/23
Posts: 44
Last seen: 24 minutes, 12 seconds
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An update. First Transfers!
-------------------- "[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate " ~ Bigfoot My LAGM 2.024
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EniQma
Registered: 11/28/23
Posts: 473
Last seen: 38 seconds
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Op updated don’t know how to link it. Just some germ pics
Nvm, thanks captain! OP up hither
Edited by EniQma (01/09/24 01:57 PM)
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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Re: LAGM v. 2.024 [Re: EniQma]
#28615298 - 01/09/24 01:51 PM (19 days, 4 hours ago) |
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Quote:
EniQma said: Op updated don’t know how to link it. Just some germ pics
Copy the link (right click on PC or Hold on Phone) the blue #xxxxxxxxx number on your OP. Choose copy link address.
You then press the globe with an infinity symbol at the top of the text box for your new post. It will open a window where you can paste the link you copied.
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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Vibetyme
Smoke 1



Registered: 06/08/21
Posts: 1,763
Loc: Florida
Last seen: 41 seconds
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Finally nocced up my plates! Better late then never!
Update
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: Vibetyme] 3
#28616274 - 01/10/24 11:21 AM (18 days, 6 hours ago) |
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Minor update with no pics, just updated to say that we've got confirmed germination on my Jambos and now every one of my varieties has sprung to life 
Mycelium is growing from a clump of spores but there is also a very small colony of bacteria growing directly on my streak line so this may be a difficult culture to clean. Time will tell and I'll give 'er hell!
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I 5318008 NOT a virgin!
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Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 4 hours
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Updated OP
Put more spores to agar this time with a swab, really hit me in that moment- you can make metric fuck tons of swabs from a single print if you're so inclined, neat.
-------------------- Intro to Shroomery The Sexy TEKs™ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
Edited by Tri-Polar (01/10/24 09:33 PM)
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SeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 2 days, 8 hours
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OP updated. I have life! My plates are a bit behind the curve, but 3/6 have some sort of germination today!
-------------------- LAGM 2024
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r0kovski
Stranger

Registered: 09/24/23
Posts: 48
Last seen: 5 hours, 13 minutes
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11/1/24 Signs of germination from both strains (Shakti and YUxGM) not fluffy white mycelium, but visible differences on top face of agar.
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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Re: LAGM v. 2.024 [Re: r0kovski]
#28617174 - 01/11/24 04:33 AM (17 days, 13 hours ago) |
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Updated me log
The slow growth is agonizing
-------------------- LAGM 2024
Thank yoU please come again FrienD
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edixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 12 hours, 23 minutes
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Re: LAGM v. 2.024 [Re: edixo] 1
#28618262 - 01/12/24 02:49 AM (16 days, 15 hours ago) |
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Quote:
edixo said: 12/01/2024
Plate 1 has some very even, solid growth going on, so did 3 transfers of that to some store-bought PCA. The homemade agar -seems- to be working, but it's slow.

-------------------- ~ LAGM 2024 ~
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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OP updated
Quote:
mycosispeon said: 01/12/24
Observations
So far, nothing on the dishes I inoculated with TAT. Fortunately, I've got germination on one of the dishes of Ghost. 
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
mycosispeon said: OP updated
Quote:
mycosispeon said: 01/12/24
Observations
So far, nothing on the dishes I inoculated with TAT. Fortunately, I've got germination on one of the dishes of Ghost. 
Fuck yeah, be patient. I'm surprised you've got something so soon
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I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: OP updated
Quote:
mycosispeon said: 01/12/24
Observations
So far, nothing on the dishes I inoculated with TAT. Fortunately, I've got germination on one of the dishes of Ghost. 
Fuck yeah, be patient. I'm surprised you've got something so soon 
After my hack job of inoculating with swabs, I was a bit concerned
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Time will tell. There's nothing you can do now anyway except sit back and observe. Even if it is mold watch it and see how it behaves so you can spot it again in the future.
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I 5318008 NOT a virgin!
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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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Minor updates
Quote:
ReverendMyc said: 2024-01-10 Definitely seeing germ on Mex Chicon Nindo and Subtrop Hindsight. Hints of germ on the final two as well.
2024-01-12 Out of town for a while and missing them already. They grow up so fast.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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