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Womble
Wombling Free

Registered: 09/12/23
Posts: 97
Last seen: 2 days, 10 hours
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Personally, I'm only taking my eyes off of my plates to check this thread and then its straight back to turning and moving them around using different lights and my phone to see if there's been any germination or further growth.
On a serious note, happy to report I do have some germination.
Also not so happy top report I think I might also have the threads first mold germination, there's an award/prize for that too right?
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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Re: LAGM v. 2.024 [Re: Womble] 2
#28610424 - 01/05/24 11:55 AM (23 days, 5 hours ago) |
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I drive truck and am gone for a week at a time. I bug my wife to send me pictures
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r0kovski
Stranger

Registered: 09/24/23
Posts: 48
Last seen: 5 hours, 13 minutes
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Hi everyone, count me in.
Strains: -Shakti -Yellow Umbo x Great Monster F2 (YUxGM) -Black cap natalensis (post in transit, thank you SupaThaRipper🤗)
I have one question. I put YUxGM spores to agar few days before 1.1., should i start with new swab to agar or I'm allowed to use few days older culture? Other strains will be put on agar soon.
My SAB, looks lame with foil and ducktape, but works.

Everytime I work in SAB, I open agar plate to check cleanliness of air. That is 3rd time and still no contaminations.

6/1/24 Shakti spores put on agar



8/1/24 YUxGM spores on agar.

11/1/24 Signs of germination from both strains (Shakti and YUxGM) not fluffy white mycelium, but visible differences on top face of agar.
27/1/24
Shakti, YUxGM T1 transfer today.
Edited by r0kovski (01/27/24 11:21 AM)
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Re: LAGM v. 2.024 [Re: r0kovski] 1
#28610566 - 01/05/24 02:07 PM (23 days, 3 hours ago) |
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 This post will serve as my Official Entry and Grow Log  
I'll be attempting a few different P. Cubensis subspecies:
JMF from MSS (sus)
- TAT from Swab
- Ghost from Swab
12/09/23
Poured agar dishes
I decided to do a 56/44 MEA mixture. My media bottles are 500ml, so the recipe was split in two batches.
1000ml distilled water 20g Telephone agar-agar 16g Light dry malt extract
Water heated in microwave; mixed with combined powder; PC for 20min @ 15psi; water bath to 120°F; poured in SAB, bottom-up in stacks of 10 (came out with 48); allowed to cool, and wrapped with cling wrap
01/01/24
Inoculated Agar
I've been having a hard time getting a good culture out of a suspect syringe I received a few weeks ago, so I decided to try one more time. In my SAB, I dispensed ~10 drops of solution directly onto the the surface of two agar dishes. The dishes were re-wrapped, labeled (D-106, D-107), and stored. In the past, I've just dumped all of the solution in one spot, but this time, I decided to put one drop in ~10 different spots.
01/05/24
Observations
I had a bitch of a time figuring out how to take images that looked like what I was seeing with my eye. With light behind? With light above? My labels didn't help matters. My camera clearly sucks balls. Here's what I ended up with:

Already there is cause for alarm... I see multiple points of germination, but also some kind of wet bacteria I think. Are these tossers?
01/09/24
Observations
Okay, I'm officially giving up on these dishes.

Spores coming in the mail compliments of bigfootscreepyuncl, I will have some new entries tonight.
01/09/24 (part DOH!)
Inoculated Agar
The disappointment of having to toss the JMF dishes was replaced with the excitement of receiving new genetics (a sincerely hardy thanks to bigfootscreepyuncl!!). So I setup the SAB and went to work. I decided to inoculate two plates each with spore swabs of both TAT and Ghost. It ended up being a bit of a hack job as I had a difficult time extracting fibers from the swab using my tweezers for the first in each set of dishes, but... mission accomplished (and :fingerscrossed:).
TAT
Ghost

01/12/24
Observations
So far, nothing on the dishes I inoculated with TAT. Fortunately, I've got germination on one of the dishes of Ghost. 
TAT
 Ghost

01/15/24
Observations
Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.
TAT
 Ghost

01/17/24
Observations
The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.
TAT
 Ghost

01/18/24
Isolation
T1s taken! Though all four of my germ plates have at least two different organisms growing on them, only the two that received the swab heads germinated good candidates for isolation. The TAT one only had a single sector that looked good, so I transferred it to a new dish. The Ghost had 3 or 4 interesting sectors, but all of them appeared to have another mold growing right behind and on top of the Cubensis mycelium, so I selected the two with the greatest margin and transferred to a new dish.
TAT
 Ghost

01/22/24
Observations
T1s looking good so far. Will definitely have to do a T2 in the coming days. The germ plates really took off since.
TAT
 Ghost

01/24/24
Isolation
The T1 dish of Ghost I made on the 18th had grown out to where the two original samples had merged and there were several great looking sectors, so I decided to make T2s. I decided to transfer samples into one new MEA agar dish with yeast added and into one without. Y'know... for science and stuff. The TAT T1 has been lagging behind a bit and like I'd posted in the AGAR thread, the germ plate for that one actually grew out to exhibit some fantastic looking rhizomorphic growth, so I decided to take another round of T1's from it. Again, I transferred a sample both to a dish with, and a dish without yeast added.
No pictures because condensation in the dishes made it difficult. They should clear up in a day or so of sitting upside-down at which point I'll post some pix.
01/27/24
Observations
The new T1s of TAT are really taking off. The original T1 of TAT has one "cord" that is breaking away from the rest. Is that just a rogue strain? T2s of Ghost are looking good. You can really see the difference in agar between the one with vs without yeast.
TAT
Ghost
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
Edited by mycosispeon (01/27/24 11:49 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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I wouldn't use any of those. Sorry homie. You can clean bacterial cultures but it can be a painstaking, long process. I usually only do that to myself if it's a rare variety or a clone culture that is worth all the work.
Personally, I'd just start fresh with whatever the mailman drops off next week. Or, if you hate the thought of wasting syringes I'd give the josex poke method a try. I've cleaned up a couple highly bacterial syringes that way...it is such an ingenious TEK
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I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said: I wouldn't use any of those. Sorry homie. You can clean bacterial cultures but it can be a painstaking, long process. I usually only do that to myself if it's a rare variety or a clone culture that is worth all the work.
Personally, I'd just start fresh with whatever the mailman drops off next week. Or, if you hate the thought of wasting syringes I'd give the josex poke method a try. I've cleaned up a couple highly bacterial syringes that way...it is such an ingenious TEK
It's all good, I'm not married to 'em. At least I know now that I can toss the suspect syringe. I can (can't) wait for the mailman to come
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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RockinRobot
Stranger


Registered: 12/08/22
Posts: 863
Last seen: 8 minutes, 50 seconds
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Updated OP
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
ReverendMyc said:Try blinking less
Holy shit it worked! We have liftoff on my bisporous plate and a minor update made to my grow-log. I'll try and post pictures tomorrow. It's just the faintest of growth and doesn't show up in any pictures, but it's there!
I'm going to need to pour some new plates soon!
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I 5318008 NOT a virgin!
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Pnin
Riz Gukgak


Registered: 07/18/23
Posts: 395
Last seen: 3 hours, 57 minutes
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OP updated with some pics of plates.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: Pnin]
#28611479 - 01/06/24 09:35 AM (22 days, 8 hours ago) |
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Very minor update just to add these germination pics of my bispo:

Not the prettiest pics or plates..I do love using yeast in my plates but damn I also miss having crystal clear plates 
Also, I'm pretty certain my eyes are just playing tricks on me but I think I might have some action on my BVI plate
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I 5318008 NOT a virgin!
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r0kovski
Stranger

Registered: 09/24/23
Posts: 48
Last seen: 5 hours, 13 minutes
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: r0kovski]
#28612296 - 01/06/24 08:30 PM (21 days, 21 hours ago) |
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I'm still not 100% sure but I believe my BVI has also sprouted and Wild Coast may be showing signs of life as well. Still nothing on NecD 
I know it's only been 6 days but has anyone made a transfer yet? I'm getting EXCITED!
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I 5318008 NOT a virgin!
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EniQma
Registered: 11/28/23
Posts: 473
Last seen: 19 seconds
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No germination over here yet
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: EniQma]
#28612817 - 01/07/24 09:51 AM (21 days, 8 hours ago) |
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Nothing worthy of a full update yet but I have confirmed germination of: Bispo, BVI and Wild Coast. I'm still hopeful but I believe my Jambo and NecD prints are no longer viable. Fingers crossed!
Unless things really start growing quickly it looks like I'll be making my first transfers next weekend
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I 5318008 NOT a virgin!
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 42 minutes
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Hold out hope creepyuncl, it's only been a handful of days
Updated me log with methods + links + a few germ pics
-------------------- LAGM 2024
Thank yoU please come again FrienD
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: mnj] 2
#28613286 - 01/07/24 05:40 PM (21 days, 12 minutes ago) |
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Minor update to add some pics. Confirmed germination on BVI, Wild Coast and Bispo is growing in nicely.
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I 5318008 NOT a virgin!
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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Womble
Wombling Free

Registered: 09/12/23
Posts: 97
Last seen: 2 days, 10 hours
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Updated post with plate progress.
Any advice around when I should think about starting T1 transfers?
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Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 4 hours
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Re: LAGM v. 2.024 [Re: Womble]
#28613624 - 01/08/24 04:43 AM (20 days, 13 hours ago) |
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Updated OP
Put spores to agar, first time using an inoculation loop so I feel like I fucked it up, also was too busy to pour new plates so I used an old PDA plate from a batch I was pretty displeased with but I couldnt care less and probably neither could the spores so here we go babyyy
-------------------- Intro to Shroomery The Sexy TEKs™ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,499
Loc: USA
Last seen: 40 minutes, 36 seconds
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I don’t use a loop anymore. Sterile swabs all day ❤️
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