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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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OP updated with pix
Quote:
mycosispeon said: 01/15/24
Observations
Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
mycosispeon said: OP updated with pix
Quote:
mycosispeon said: 01/15/24
Observations
Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.
Very nice! Looks like you'll definitely have enough myc to work with but you'll likely need to do a couple transfers. I'm sure you're aware but you've got some major bacteria colonies on some of your plates (bacteria in isolated colonies is usually a non-issue at all to work around).
The spot(s) that have me the most concerned are on your Ghost swab plate are circled in blue, particularly the patch of mycelium forming below/behind the swab stick. I would be looking to transfer from the areas circled in red, and avoid/keep an eye on the areas circled in blue:

If you have the option, meaning, if the fuzz underneath the stick doesn't start changing colors I would wait a little long to let the red areas grow out a little further to before transferring. But, if you have to transfer sooner to avoid contaminants then that's the name of the game.
Looking pretty good
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I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: OP updated with pix
Quote:
mycosispeon said: 01/15/24
Observations
Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.
Very nice! Looks like you'll definitely have enough myc to work with but you'll likely need to do a couple transfers. I'm sure you're aware but you've got some major bacteria colonies on some of your plates (bacteria in isolated colonies is usually a non-issue at all to work around).
The spot(s) that have me the most concerned are on your Ghost swab plate are circled in blue, particularly the patch of mycelium forming below/behind the swab stick. I would be looking to transfer from the areas circled in red, and avoid/keep an eye on the areas circled in blue:

If you have the option, meaning, if the fuzz underneath the stick doesn't start changing colors I would wait a little long to let the red areas grow out a little further to before transferring. But, if you have to transfer sooner to avoid contaminants then that's the name of the game.
Looking pretty good 
Yep I keyed in on those red areas too. I'll keep an eye on it. What do you think about the other 3 dishes? It looks like both of the Ghost dishes have some of that "wet" mold. The two TAT dishes don't have any of that, but whatever is growing looks a little fuzzy, like cottony.
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
Edited by mycosispeon (01/15/24 11:53 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
mycosispeon said:
Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: OP updated with pix
Quote:
mycosispeon said: 01/15/24
Observations
Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.
Very nice! Looks like you'll definitely have enough myc to work with but you'll likely need to do a couple transfers. I'm sure you're aware but you've got some major bacteria colonies on some of your plates (bacteria in isolated colonies is usually a non-issue at all to work around).
The spot(s) that have me the most concerned are on your Ghost swab plate are circled in blue, particularly the patch of mycelium forming below/behind the swab stick. I would be looking to transfer from the areas circled in red, and avoid/keep an eye on the areas circled in blue:

If you have the option, meaning, if the fuzz underneath the stick doesn't start changing colors I would wait a little long to let the red areas grow out a little further to before transferring. But, if you have to transfer sooner to avoid contaminants then that's the name of the game.
Looking pretty good 
Yep I keyed in on those red areas too. I'll keep an eye on it. What do you think about the other 3 dishes? It looks like both of the Ghost dishes have some of that "wet" mold. The two TAT dishes don't have any of that, but whatever is growing looks a little fuzzy, like cottony.

The area in blue is a large, isolated bacteria colony. Is that what you were referring to as the wet mold? Those are easy to work around. Basically just don't touch them. Bacteria doesn't move very fast or sporulate so as long as it's away from your culture so is generally nothing to worry about. Bacteria within your culture is another story.

That blue circle is gonna be real nice in a few days. Be patient and transfer from there (assuming nothing weird happens between now and then).

I would transfer from any of these red areas. It all looks pretty clean for a germ plate. But, if it comes down to it I'd definitely use the mycelium from the swab plate. And why the fuck did you stab the plate so many times?
I'm just bustin your balls. Swabs are a MF to deal with if you're not used to them. That's why if I have 2 I'll just jam the first one in and if it doesn't work then on the second swab I'll tease out fibers and be a little more delicate.
Everything looks good amigo keep us posted!
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I 5318008 NOT a virgin!
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 34 minutes
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OP updated.
Dealing with bacteria on my Pan Cyan plates. Attempting to clean it up. Plan to do 1 transfer and then transfer again to water agar asap. I'm leaving for work tomorrow, so it will have to wait a week.
Edited by CaptainPuffy (01/15/24 01:08 PM)
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said:

I would transfer from any of these red areas. It all looks pretty clean for a germ plate. But, if it comes down to it I'd definitely use the mycelium from the swab plate. And why the fuck did you stab the plate so many times?
I'm just bustin your balls. Swabs are a MF to deal with if you're not used to them. That's why if I have 2 I'll just jam the first one in and if it doesn't work then on the second swab I'll tease out fibers and be a little more delicate.
Everything looks good amigo keep us posted!
LOL yeah yeah yeah. I hear ya. Hack job, like I said. I couldn't for the life of me get the gd fibers to come off of my tweezers!
It's all good. I'll see how they look tomorrow or Wed
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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LyleChipperson


Registered: 09/29/23
Posts: 61
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Can I join in? I got new spores last week for my second grow ever so I put them to agar.
1/11
Inoculated 6 plates each with Burmese PE spore print and Natalensis swab. Agar recipe was PDA from this forum. 5 days in and there is no growth yet.
1/18 There is visible growth on the Natalensis swab plate, all the others have only developed small contaminate blotches so far.

1/19
The BPE is still showing no signs of growth, so today I'll get more plates started with Pan Cyans and PF Redspore. I wasted 2 months trying to clean up dirty GT cultures unsuccessfully, so this time I'll get everything I have on agar and I'll pick the cleanest growth in the end. Hopefully this carpet bombing approach will give better results.
1/25
Took 10 transfers from the Natalensis swab plate, all the other plates still have nothing going on. I couldn't see the tiny contaminate well in the SAB so I may have transferred it along to some plates, but hopefully I will get enough clean ones out of the 10 to keep it going. This time I tried oven sterilizing my plates for 90 mins at 200 c and my agar for 1 hour in the PC to see if it will make a difference, since I didn't have that contaminate before, but it was present in my last 2 sessions and there have been no changes in the way I do it.
Edited by LyleChipperson (01/26/24 07:32 AM)
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 2 hours, 43 minutes
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Of course! Welcome
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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Nikoyo
FUN GUY



Registered: 01/29/17
Posts: 288
Last seen: 6 days, 14 minutes
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Small update on the op with some pics. Taking t2s on some of the cultures tonight; my mvp plates are far ahead of the rest
-------------------- Gettin back to work
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AndImTheHighOne
Stranger


Registered: 12/06/23
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Last seen: 24 minutes, 4 seconds
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Re: LAGM v. 2.024 [Re: Nikoyo]
#28623671 - 01/16/24 03:55 PM (12 days, 1 hour ago) |
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I have typed out a nearly finished update twice today only to forget about it and have it deleted by a page refresh 😭 From now on I'm starting every update in my notes and only adding pictures at the end. Bear with me guys, I will re-type it once again and get an update out today.
-------------------- "[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate " ~ Bigfoot My LAGM 2.024
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AndImTheHighOne
Stranger


Registered: 12/06/23
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Updated my OP. Finally.
Quote:
AndImTheHighOne said: **UPDATE 1/16/24** (!) All T1s Complete (!), First Plates Contam, Bad Ecuador, and My Plan Moving Forward
Alright, alright, alright we've got a lot to cover as this is my first update in A WEEK! For anyone who is following along, I certainly don't want to make you wait too long between updates and lose your interest. But what is LAGM without a bit of suspense, right?!
So without further ado, I present my eight T1 transfers:

Organized by row, from top to bottom, we have: Tosohatchee, Hanoi, P. natalensis, and Golden Koh F2
Continued...
-------------------- "[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate " ~ Bigfoot My LAGM 2.024
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SeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 2 days, 8 hours
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OP updated. Making a risky bet on some LC :P
I'm being very lazy here. I don't feel like making more agar plates, but happen to have some ready-to-go LC vials sitting around. So, "transfer" to LC, everything should be fine. nothing could possibly go wrong. And if it does, I mean, I guess that just means I need to do the thing I wasn't doing anyways. No real loss but time.
-------------------- LAGM 2024
Edited by SeventhMushroom (01/17/24 01:20 AM)
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Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 4 hours
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Updated OP
good news we got germination, bad news im sick af with covid
Win some lose some
-------------------- Intro to Shroomery The Sexy TEKs™ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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OP Updated...
The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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fiddle_head
I'm not the dude, guy



Registered: 08/05/08
Posts: 1,877
Last seen: 8 hours, 40 minutes
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Re: LAGM v. 2.024 [Re: Noncense] 2
#28624718 - 01/17/24 03:29 PM (11 days, 2 hours ago) |
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Everyone is looking great so far. I´m excited for my second lagm to begin i just have to finish my current projects. alot of my germ plates will be f1 and f2 generations of my current grows. there are some i am germing like kodama which i have not grown previously. best of luck to everyone and cant wait to join in.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
mycosispeon said: OP Updated...
The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.
Those satellite colonies on your ghost plate have me a little concerned though. Did the swab roll around on the plate at all or did you streak it on the surface? If not I'd be looking into transferring as soon as you have time. If those satellites are in fact mold, once they start sporulating it will get a lot harder to clean a culture.
Lookin' good though!
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I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: OP Updated...
The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.
Those satellite colonies on your ghost plate have me a little concerned though. Did the swab roll around on the plate at all or did you streak it on the surface? If not I'd be looking into transferring as soon as you have time. If those satellites are in fact mold, once they start sporulating it will get a lot harder to clean a culture.
Lookin' good though!
Shit... No, the swab didn't roll around, nor did I streak. I'm pouring new agar dishes right now. Hopefully, if it IS mold, it doesn't sporulate overnight.
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
mycosispeon said:
Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: OP Updated...
The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.
Those satellite colonies on your ghost plate have me a little concerned though. Did the swab roll around on the plate at all or did you streak it on the surface? If not I'd be looking into transferring as soon as you have time. If those satellites are in fact mold, once they start sporulating it will get a lot harder to clean a culture.
Lookin' good though!
Shit... No, the swab didn't roll around, nor did I streak. I'm pouring new agar dishes right now. Hopefully, if it IS mold, it doesn't sporulate overnight.
don't let me get you worked up, I've already cried mold once but this time I'm more certain...the neat thing about agar is you can't really screw up on an irreparable level. You have as many 'do overs' as you want up until you drop a wedge into the grain jar. Nothing to lose any sleep over, just transfer away from it as soon as you get a chance 
I didn't even have time look at my plates yesterday. I'm sure they're due for a transfer which works out pretty nicely. I've got 5 varieties and 5 poured plates left. Now I just need to find a little time to make some cuts. That won't be today though.
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I 5318008 NOT a virgin!
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Major update without any pics - Bigfoots going back to spore because I'm pretty certain I have only been growing (and transferring) mold at this point in time lol. I'm waiting to hear back from some experts on the species but I know what I'm looking at...
This isn't exactly how I wanted to spend my day recovering from an adderall relapse bender but it seems like a justified and fitting punishment if I'm being honest. It's just one of those days!
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I 5318008 NOT a virgin!
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EniQma
Registered: 11/28/23
Posts: 473
Last seen: 30 seconds
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Quote:
bigfootscreepyuncl said: Major update without any pics - Bigfoots going back to spore because I'm pretty certain I have only been growing (and transferring) mold at this point in time lol. I'm waiting to hear back from some experts on the species but I know what I'm looking at...
This isn't exactly how I wanted to spend my day recovering from an adderall relapse bender but it seems like a justified and fitting punishment if I'm being honest. It's just one of those days!
Haha bro I knew you were speedin your tits off with all tho posting you were doing! I was also wondering how the hell you lucked out with such fast colonizers.
Don’t beat yourself down. Shit happens
Edited by EniQma (01/18/24 02:03 PM)
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