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RockinRobot
Stranger


Registered: 12/08/22
Posts: 863
Last seen: 9 minutes, 9 seconds
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Updated OP with new pics
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edixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 12 hours, 23 minutes
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Re: LAGM v. 2.024 [Re: edixo] 1
#28613877 - 01/08/24 09:26 AM (20 days, 8 hours ago) |
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Quote:
edixo said: 08/01/2024
Liftoff! We have germination on plate 1! Now we wait for more.

-------------------- ~ LAGM 2024 ~
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: Womble]
#28614060 - 01/08/24 12:15 PM (20 days, 5 hours ago) |
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Quote:
Womble said: Updated post with plate progress.
Any advice around when I should think about starting T1 transfers?
It's hard to say. I usually recommend to take a transfer as soon as you're certain it's mushroom mycelium and are able to make the transfer without any risk of grabbing spores in the process. But, on the other hand I do sometimes like to let it grow out and better establish itself incase it wants to present different growth habits.
It's hard to see what's going on in your plate so it's hard for me to give you better advice Whenever you choose to take transfers though make sure you take several of each. Frequently I will put 2 or 3 transfer pieces to the same plat and make 2 plates. You have to work a little faster doing this method as the plates will become overgrown quickly, but it gives you a wide variety or growth to select from and it increases you chances of being able to select clean mycelium.
Good work so far, keep it up!
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I 5318008 NOT a virgin!
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Womble
Wombling Free

Registered: 09/12/23
Posts: 97
Last seen: 2 days, 10 hours
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Quote:
bigfootscreepyuncl said:
Quote:
Womble said: Updated post with plate progress.
Any advice around when I should think about starting T1 transfers?
It's hard to say. I usually recommend to take a transfer as soon as you're certain it's mushroom mycelium and are able to make the transfer without any risk of grabbing spores in the process. But, on the other hand I do sometimes like to let it grow out and better establish itself incase it wants to present different growth habits.
It's hard to see what's going on in your plate so it's hard for me to give you better advice Whenever you choose to take transfers though make sure you take several of each. Frequently I will put 2 or 3 transfer pieces to the same plat and make 2 plates. You have to work a little faster doing this method as the plates will become overgrown quickly, but it gives you a wide variety or growth to select from and it increases you chances of being able to select clean mycelium.
Good work so far, keep it up!
I appreciate that the pics are really not that helpful, I really struggled with getting clear photographs. I'll have to have a play around with that.
Thanks for the pointers, I feel quite confident in what looks good and what doesn't and I know it takes time and experience, as with everything else in this hobby, so I don't feel like I'm completely fumbling about in the dark but it's good to get a feel for what works for everyone else and take it from there.
I'll pour more plates and take transfers tomorrow I see how that goes.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM v. 2.024 [Re: Womble] 1
#28614096 - 01/08/24 01:10 PM (20 days, 4 hours ago) |
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very minor update to report the appearance of satellite growth on my Wild Coast plate which I actually don't think is contamination though! Also I believe I have germination on NecD finally
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I 5318008 NOT a virgin!
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Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 4 hours
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Quote:
SupaThaRipper said: I don’t use a loop anymore. Sterile swabs all day ❤️
Yeah thats gonna be my go to from now on, was kicking myself for not using one like halfway through the process haha
-------------------- Intro to Shroomery The Sexy TEKs™ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
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edixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 12 hours, 23 minutes
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Re: LAGM v. 2.024 [Re: edixo] 1
#28614747 - 01/09/24 02:56 AM (19 days, 14 hours ago) |
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Quote:
edixo said: 09/01/2024
Really nice growth on plate 1 already, so did a small transfer to some homemade agar to see if it work. If it does, I'll try to put it to grain.

-------------------- ~ LAGM 2024 ~
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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Quote:
Tri-Polar said:
Quote:
SupaThaRipper said: I don’t use a loop anymore. Sterile swabs all day ❤️
Yeah thats gonna be my go to from now on, was kicking myself for not using one like halfway through the process haha
Swabs:
I've never used one
What does everyone like about them?
When you're using a swab, what's your preferred source of spores, meaning swiping a fresh gill, swiping a print, or shooting spore solution onto the tip?
-------------------- LAGM 2024
Thank yoU please come again FrienD
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 1 hour, 31 minutes
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Re: LAGM v. 2.024 [Re: mnj] 5
#28614821 - 01/09/24 05:31 AM (19 days, 12 hours ago) |
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Quote:
mnj said:
Swabs:
I've never used one
What does everyone like about them?
When you're using a swab, what's your preferred source of spores, meaning swiping a fresh gill, swiping a print, or shooting spore solution onto the tip?
I hate swabs, and wouldn’t use them if I didn’t have to. I only use them to grab spores from variants that won’t drop them.
I’ve tried using them instead of a loop to transfer from a print to agar. They work, but hold a lot of spores in the tip. I end up having to break it off and shove it in. A loop works well, is easy to flame sterilize, and is not yet another disposable thing to get tossed in the landfill. I don’t know why you would ever have to shoot spore solution onto a swab when you can just drop it onto a plate.
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Silentraindrops
mushlove student

Registered: 12/23/23
Posts: 222
Loc: pnw
Last seen: 17 hours, 50 minutes
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Quote:
RoscoeReturns said:
Quote:
mnj said:
Swabs:
I've never used one
What does everyone like about them?
When you're using a swab, what's your preferred source of spores, meaning swiping a fresh gill, swiping a print, or shooting spore solution onto the tip?
I hate swabs, and wouldn’t use them if I didn’t have to. I only use them to grab spores from variants that won’t drop them.
I’ve tried using them instead of a loop to transfer from a print to agar. They work, but hold a lot of spores in the tip. I end up having to break it off and shove it in. A loop works well, is easy to flame sterilize, and is not yet another disposable thing to get tossed in the landfill. I don’t know why you would ever have to shoot spore solution onto a swab when you can just drop it onto a plate.
never used a swab everything you said is about why I don't go through the effort of a clean swab... I like loops and MSS. I can see why you'd use them for gathering the spores tho i will try that one day soon .
Squeezing the side of a syringe for a drop is so easy .... I even like turning my print into a syringe . Idk if my prints are very clean but its fun lol. My b+ print was surprisingly clean I didn't get a ton of contams when i was testing all sorts of methods. idk. i did loop , sprinkle/tap , mss, hell i let the water run off the foil into the agar lol.... I didn't use a q tip... that's what would of done it :P.
Edited by Silentraindrops (01/09/24 11:14 AM)
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
Silentraindrops said:Idk if my prints are sterile but its fun lol
Prints, no matter how clean they are, will not ever be sterile. For one, if it were truly sterile your spores would no longer be viable. Plus, mushrooms are grown in open air and handled a significant amount in order to harvest the spores. Even if the fruits were grown in a positive pressure clean-room it is still highly likely that there would be some bacteria and/or mold present.
In this hobby there is a huge difference between clean and sterile and it's critical to know the difference.
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I 5318008 NOT a virgin!
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,499
Loc: USA
Last seen: 40 minutes, 55 seconds
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Re: LAGM v. 2.024 [Re: mnj]
#28614972 - 01/09/24 08:41 AM (19 days, 9 hours ago) |
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I’ll swab gills or a print and both work extremly well. I still have my loop. Need to throw that thing in the trash 😂 useless pos lol. If I use a mss, i just squirt directly to agar. Loops are shit for grabbing spores compared to swabs.
To test my statement, take a spore print that you think has been wiped completely clear of all spores. Now use a swab on it, bet you’ll fill that swab right up.
@roscoe, yeah I definitely break my swabs off and leave them in the dish. You’re definitely right about that. They do hang on. I’ll usually swipe a dish, and then on a second dish, I’ll break the swab off. They both usually germinate though. Sometimes at the same time. Sometimes one before the other or vice versa
Edited by SupaThaRipper (01/09/24 08:47 AM)
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 644
Loc: Inner Space
Last seen: 8 hours, 43 minutes
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Thanks for the replies y'all.
Silenraindrops, I never thought of squeezing the sides of a syringe to get a small drop out, might have to give that a go. But would the syringe suck in ambient air to replace the loss of mss when I release my squeeze? I imagine that's why the plunger is used.
5 out of 8 plates I streaked with the loop have not germinated, but also haven't shown any contam, so I may open them back up and shoot a drop off mss in there.
-------------------- LAGM 2024
Thank yoU please come again FrienD
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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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Quote:
RoscoeReturns said: I hate swabs, and wouldn’t use them if I didn’t have to. I only use them to grab spores from variants that won’t drop them.
I’ve tried using them instead of a loop to transfer from a print to agar. They work, but hold a lot of spores in the tip. I end up having to break it off and shove it in. A loop works well, is easy to flame sterilize, and is not yet another disposable thing to get tossed in the landfill. I don’t know why you would ever have to shoot spore solution onto a swab when you can just drop it onto a plate.
x 100.
I use them to get spores from non-dropping varieties but then usually resort to making brf pucks to germinate them. Then transfer to agar to clean, so just extra steps. Plus, you basically get one or at most two uses out of a swab while a loop on a print can be used many times. They work from prints, but seem wasteful to me since most of the spores end up staying on the swab. EDIT: plus more of a pita to ship than prints. Love to share, hate shipping and handling and special non-bendable postage and envelopes required with swabs.
Speaking of which, that is what I did last night with the remainder of a couple of swabs that also went to agar for LAGM. The remaining spores on the swabs got dipped in sterile water and shoved into bfr pucks. As well as a few transfers from two of the more aggressive LAGM plates.
Quote:
ReverendMyc said: 2024-01-08 Transferred the most aggressive two plates to some new plates. Mexicana Chicon Nindo from print & Pan Cyan Bunnell from print Still waiting on the swabs to get started.

Also did some other things that I started before the 1st.

OP updated.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (01/09/24 09:01 AM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,499
Loc: USA
Last seen: 40 minutes, 55 seconds
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I’ll usually hand out swabs in my giveaways. Can make dozens off one print vs taking dozens of prints. Saves a lot of time. I also started getting 3 or 4” swabs. Which have been fine st the post office for sending as regular, flexible mail. For the longer swabs though, you’re right. You need to pay $1 for the non flex mail, otherwise they’ll send them through the rollers and break the sticks. You can also swab to swab to get more spores as well. I’ve done hundreds of swabs and have only ever had one set not germinate. Same thing can happen with prints though. I’ve had prints not germinate as well. It’s also why I recommend breaking the swabs off in the dish and smashing them in the agar. This is the way to go. Swipe one plate, smash another.
Here’s a picture of an aggressive swab I had just for shits and giggles on 1% lol

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ReverendMyc

Registered: 03/29/19
Posts: 1,495
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Ya. Just banter. I know that swabs work fine, I just prefer prints and loops. I have success and failures with both depending a lot on age and species. I will have to look for the short flexible swabs in the future for PE varieties.
This is a Jack Frost from swab plate that I took transfers from last night.
 If I do swabs (not from prints), I usually wet them in water that I sterilized in cryo tubes for the purpose. Then tear off little pieces and shove them into the agar, or a brf puck if they are stubborn. Works all the time most of the time.
Just sharing why I prefer one method over the other for those who don't have our level of experience that will read our witty repartee.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (01/09/24 09:33 AM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,499
Loc: USA
Last seen: 40 minutes, 55 seconds
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Oh dude I’m strictly here just to argue 🤣 no I know man it all comes down to preference and we all have our own methods for sure. Prints are definitely better when it comes to always having spores to go back to as well!
So water on the swab is the trick for pulling off fibers huh. I’ve tried doing it dry before and couldn’t understand how people make it work lol.
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,499
Loc: USA
Last seen: 40 minutes, 55 seconds
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Dude don’t give up on those plates! Transfer to brf pucks!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 22 hours, 49 minutes
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Quote:
SupaThaRipper said: Dude don’t give up on those plates! Transfer to brf pucks!
I actually don't NEED them anyway. I had some better looking ones from another MSS syringe I'd started earlier in December, so they don't qualify for LAGM, but I put it to grain last night
 J-014 thru J-017 taken 01/08/24
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
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