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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28439813 -

tryptkaloids said:
You can't see viability like that. Only way is to try and fruit it.
Could always just take it to a small cake or bottle tek and attempt to tip fruit to save some time



Ive ran this and tons of other cultures over the past ~9 months. I get good looking spawn but when its spawned itll either contam or just sit there for months without ever fruiting. Tidal wave, APE, penis envy, Natalensis, all the same thing every time. Dont understand whats going wrong. Ive cultivated in the past and did not have these issues. Tried top fruiting 2 good looking jars and even they came down with green in the coir so they were tossed.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28439876 -

Sounds like it could be many things. Check all your lids, double check your grain prep and conditions. Take a video of your sab work and look for errors. Let us look for errors.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28440029 -

PC cooling down now, excited to use my new pitcher :pipesmoke:



--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28440408 -

First ever transfer in the bag! (B+)
The little black dot you see in the bottom left is soot from my scalpel I believe.
The little triangle piece is where I found a little pink dot of contam. I cut it out in hopes it will stop spreading it showed up about 2 days ago and didn’t grow any bigger so maybe unnecessary but whatever I’m a noob just starting out. :rockon:


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28440437 -

That was a bacterial satellite.  Wasn't going to spread


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28440440 -

I didn’t think it would but just in case thank you for the info. I’m just learning.


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar] * 3
    #28440592 -

0mar said:
First ever transfer in the bag! (B+)
The little black dot you see in the bottom left is soot from my scalpel I believe.
The little triangle piece is where I found a little pink dot of contam. I cut it out in hopes it will stop spreading it showed up about 2 days ago and didn’t grow any bigger so maybe unnecessary but whatever I’m a noob just starting out. :rockon:




You should get used to making your transfer pieces a fraction of that size.



--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #28440603 -

Will do. Any reason as to why? Always learning.


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar] * 1
    #28440616 -

It's a good practice to have for when you may have a more contaminated plate.

Some contaminants are hidden, and by using larger transfer pieces, you may well be transferring some of those contaminants.

A smaller piece narrows genetic variation - this can be both favoured and unfavoured, however, I will give an example: I believe it may be user Josex that mentioned this. In a method of his he uses a syringe and needle to extract a microscopic piece of tissue. He said that in doing this, he has consistently gotten canopies and a lack of variation in fruits (all tall vs some small, medium, large). Does that make sense?

Also, as a beginner, cutting across a larger surface area may mean you're not being so careful of your tool and hand placement.

And again, as a beginner, you do not yet have a trained eye so you may end up transferring unwanted nasties. If you get mostly myc in your small wedge, but a small part of a contaminant, there's a higher chance that the recovering plate will give you better luck of recovering the mycelium you want.

And finally, you will get more out of your mycelium / plates.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28440620 -

0mar said:
First ever transfer in the bag! (B+)
The little black dot you see in the bottom left is soot from my scalpel I believe.
The little triangle piece is where I found a little pink dot of contam. I cut it out in hopes it will stop spreading it showed up about 2 days ago and didn’t grow any bigger so maybe unnecessary but whatever I’m a noob just starting out. :rockon:




Hell yeah! Did my first transfers today too. These are natalensis and it seems aggressive. My other cups are cubes and they've barely germinated.


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The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #28440692 -

Guerrilla said:
It's a good practice to have for when you may have a more contaminated plate.

Some contaminants are hidden, and by using larger transfer pieces, you may well be transferring some of those contaminants.

A smaller piece narrows genetic variation - this can be both favoured and unfavoured, however, I will give an example: I believe it may be user Josex that mentioned this. In a method of his he uses a syringe and needle to extract a microscopic piece of tissue. He said that in doing this, he has consistently gotten canopies and a lack of variation in fruits (all tall vs some small, medium, large). Does that make sense?

Also, as a beginner, cutting across a larger surface area may mean you're not being so careful of your tool and hand placement.

And again, as a beginner, you do not yet have a trained eye so you may end up transferring unwanted nasties. If you get mostly myc in your small wedge, but a small part of a contaminant, there's a higher chance that the recovering plate will give you better luck of recovering the mycelium you want.

And finally, you will get more out of your mycelium / plates.



Very informative thank you for this! This is the information I love reading.


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia]
    #28440695 -

Damn that’s some beautiful plates there.


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28440884 -

Water agar.



--------------------
Somewhere between chaos and disorder.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Camera93]
    #28441102 -

Camera93 said:
PC cooling down now, excited to use my new pitcher :pipesmoke:





Is the pitcher PC'd as well? Haven't used a pitcher before only hot poured from media bottles after PC

Edited by CocaineBuffet (08/20/23 08:51 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet] * 2
    #28441116 -

Yup,it's the sorta top center vessel in the PC
Just foil over the lid, you can kinda make out the spout outline



--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


Edited by Camera93 (08/20/23 09:22 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Camera93]
    #28441142 -

Thats a neat trick thank you!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet] * 1
    #28441639 -

It has taken a week but clone plate is starting to germinate.. with aerial mycelium




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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28441747 -

CocaineBuffet said:
Thats a neat trick thank you!



Ditto
I likes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Culliganman360]
    #28441774 -

TedsDead got me hip to them!

:cheers:


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Camera93]
    #28441995 -

Camera93 said:
Yup,it's the sorta top center vessel in the PC
Just foil over the lid, you can kinda make out the spout outline





Where did you get it? Me likey.


--------------------
Somewhere between chaos and disorder.

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Shop: Myyco.com APE Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


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