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Re: AGAR ENVY! (Anything and All things agar!) [Re: Horsewithnoname] * 6
    #28237559 -

Horsewithnoname said:
Quick agar noob question: I sometimes see T1, T2, T3, etc for talking about agar. I assume we're talking about the transfer count since the first agar plate was inoculated, right?



Yes. If you put spores on agar, that's a "germ plate" (germination plate.) That would be T0, but I don't often see it called that. Just "germ plate."

Take a transfer from that, put on new plate, that's T1. Let that grow out take a transfer from T1 to a new plate, that new plate is T2. etc.

Transfers are often used to get clean mycelium to send to grain. Or to clone a good pheno.

F1, F2, etc, doesn't have to involve agar, and is used to stabilize a phenotype. Like if you have one mutant show up and you like it, it's big, or strong, fat, grows fast, looks cool, etc, you take spores from that, (send to agar just to clean up), send T1 or so to grain and grow out to shrooms, then the spores from that shroom grow is F1. Shrooms grown from spores of that are F2, as explained above. There is no F0, that's called “P” for parental generation.
https://biologydictionary.net/f1-generation/

Do that 6 to 8 times and sometimes it will get spores with a lot of that pheno showing up in a stable way. That's part of how new "varieties" (wrongly called "strains") are made. If you did it 6 times that would be called F6.

The agar is good to clean up for stabilizing, but not needed. Correct me if I'm wrong fahtster, but I think fahtster stabilizes new varieties without even using agar.

Though agar is highly recommended for anyone starting out, and is still used constantly by most excellent growers here. Someone starting should be doing transfers, T1, maybe T2.

F1, F2, for noobs, not so much. That's better after you've mastered the basics.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (03/20/23 07:04 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28237646 -

Oh wow. Thank you so much for the quick lesson! :heart:


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---------------------------

Horsie's Distilled TEKs
Grow log 2023: New beginnings
Homemade malt extract because what you bought was not malt extract but malted barley in some form TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Horsewithnoname] * 6
    #28237864 -

These Chitwan have been generous with the plate pins. These have cleaned up nicely and should do well.



--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] * 1
    #28238316 -

Scemilope said:
These Chitwan have been generous with the plate pins. These have cleaned up nicely and should do well.




Ive always had amazing luck with chitwans. Literally spore syringe into grain got multiple flushes once spawned to shoeboxes then cloned amazingly. Great strain :awecluster:


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:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix] * 2
    #28238328 -

Chitwan popped my cherry :love:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: stubb] * 3
    #28238336 -

Damn wish i could say the same. I did some unnamed field shrooms that were over grown and black from sporulation.

My Chitwans from 21:


My lovely ex doing some chitwan chakra cleansing :heart:


And the clones... Wish I still had these tbh, oh how things change am I right? :smirk:


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


Edited by Ababyphoenix (03/20/23 07:37 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix] * 1
    #28238355 -

Now that’s a happy trail!


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Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix]
    #28238362 -




Ive always had amazing luck with chitwans. Literally spore syringe into grain got multiple flushes once spawned to shoeboxes then cloned amazingly. Great strain :awecluster:



Well, that's welcome news. 💜 Those are beauties! My tubs are super spore coated and ugly. These things drop spore like I've never seen. 😂
I have some fruiting now, this pin came from a clone of those. I'll be sending lil buddy soon.


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

Edited by Scemilope (03/20/23 07:48 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope]
    #28238381 -

Scemilope said:

Ive always had amazing luck with chitwans. Literally spore syringe into grain got multiple flushes once spawned to shoeboxes then cloned amazingly. Great strain :awecluster:



Well, that's welcome news. 💜 Those are beauties! My tubs are super spore coated and ugly. These things drop spore like I've never seen. 😂
I have some fruiting now, this pin came from a clone of those. I'll be sending lil buddy soon.</font></font>


Thanka thanks. and yeah they really do I tried to get them right before veil breakage

Hells Yess

:snoopyes:


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


Edited by Ababyphoenix (03/20/23 08:02 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale] * 1
    #28238385 -

Eugene Gesuale said:
Now that’s a happy trail!


Right?!? :tellmeastory:

God I miss that woman. :crazy:


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix]
    #28238783 -

Question for everyone:

What do you do with your T1/T2 plates once you've taken your transfer(s) from them?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 2
    #28238796 -

If they weren’t good enough to send and I’ve already taken transfers oftentimes I just toss them. You can keep them around to see if they make pins, but if they weren’t clean then I don’t see the point.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 3
    #28238811 -

Depends on what you want to do and how big you want to go. If ur t2s are clean and ur pulling good myc off them you can just in theory make more t3s and sending to grain. Or take ur t3s and make a bunch of t4s. Its all about how comfortable u are with ur contam levels and what you want to do. If anything fridge them till you decide you want to work with them :shrug:

but if contamed just work past them/trash them. eventually petris pile up if u don't

edit: keeping earlier iterations around can help go back closer to MS if ur genetics eventually don't turn out. Keeping them around if ur not drowning in petris/projects could save u from having to go back to MS if u want to find different genetics


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:


Edited by Ababyphoenix (03/20/23 11:05 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 1
    #28238820 -

I keep mine until I need to reclaim plates. They can produce pins to play with. After that... I have a hole out back in trying to fill up.


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope]
    #28238825 -

Scemilope said:
These Chitwan have been generous with the plate pins. These have cleaned up nicely and should do well.




If that was mine I'd post it also to the Daily Plin:
https://www.shroomery.org/forums/showflat.php/Number/28190507/fpart/2/vc/1


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28238831 -

Smellyhobbit said:
If they weren’t good enough to send and I’ve already taken transfers oftentimes I just toss them. You can keep them around to see if they make pins, but if they weren’t clean then I don’t see the point.


Ababyphoenix said:
Depends on what you want to do and how big you want to go. If ur t2s are clean and ur pulling good myc off them you can just in theory make more t3s and sending to grain. Or take ur t3s and make a bunch of t4s. Its all about how comfortable u are with ur contam levels and what you want to do. If anything fridge them till you decide you want to work with them :shrug:

but if contamed just work past them/trash them. eventually petris pile up if u don't

edit: keeping earlier iterations around can help go back closer to MS if ur genetics eventually don't turn out. Keeping them around if ur not drowning in petris/projects could save u from having to go back to MS if u want to find different genetics




I should have been more clear, I meant this question assuming all plates are clean.

I started with several LC syringes -- I made a streak plate for each and then a drop in the middle of two t0s, so 3 plates total per syringe. Streaks and t0s all came back clean. I initially took 2 t1s per syringe 10 days ago. I then went back and took additional t1s 4 days later after seeing the myc rope up towards the outer edges of the agar

I'm done taking transfers from the plates but am trying to determine if it is worth throwing those plates to grain given that everything has been clean as a whistle and has pieces of nice looking growth on each plate

Edited by tholos (03/20/23 11:17 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 1
    #28238858 -

Clean? Send.


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🅃 🄴 🄰 🄼  🄲 🅄 🅁 🄸 🄾 🅄 🅂

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Re: AGAR ENVY! (Anything and All things agar!) [Re: dowodenum] * 1
    #28238880 -

What are you going to do with a clean plate if you aren't going to use it inoculate grains or LC? Either use it or let the plate dry out and be of no use :shrug:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28239337 -



Bottom of a no pour plate. 1% MEA. T0. Multispore. Inoculated with a tiny piece of PF tek cake. 7 days old. Already taken transfers from 3-4 o'clock. Does it look like satellite mold at 12 o'clock? Guessing that's what's preventing growth in that direction. Otherwise does it appear healthy? Just want confirmation. Will post again when transfers grow out.


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Edited by AspectOfTheCreator (03/21/23 05:36 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28239653 -

Hi everyone,

Any idea what's going on here? What is this myc trying to run from?
I keep thinking the agar surface was too wet and it's trying not to drown.

This t2 plate is 18 days old going nowhere. Seemed it had no contam, but when I opend it up to toss this it smelled a bit cheesy.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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