AGAR ENVY! (Anything and All things agar!)

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,595
Loc: Hollywoo

Last seen: 12 hours, 34 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Horsewithnoname]

6
#28237559 - 03/20/23 02:23 AM (3 years, 6 months ago)

Horsewithnoname said:

Quick agar noob question: I sometimes see T1, T2, T3, etc for talking about agar. I assume we're talking about the transfer count since the first agar plate was inoculated, right?

Yes. If you put spores on agar, that's a "germ plate" (germination plate.) That would be T0, but I don't often see it called that. Just "germ plate."

Take a transfer from that, put on new plate, that's T1. Let that grow out take a transfer from T1 to a new plate, that new plate is T2. etc.

Transfers are often used to get clean mycelium to send to grain. Or to clone a good pheno.

F1, F2, etc, doesn't have to involve agar, and is used to stabilize a phenotype. Like if you have one mutant show up and you like it, it's big, or strong, fat, grows fast, looks cool, etc, you take spores from that, (send to agar just to clean up), send T1 or so to grain and grow out to shrooms, then the spores from that shroom grow is F1. Shrooms grown from spores of that are F2, as explained above. There is no F0, that's called “P” for parental generation.
https://biologydictionary.net/f1-generation/

Do that 6 to 8 times and sometimes it will get spores with a lot of that pheno showing up in a stable way. That's part of how new "varieties" (wrongly called "strains") are made. If you did it 6 times that would be called F6.

The agar is good to clean up for stabilizing, but not needed. Correct me if I'm wrong fahtster, but I think fahtster stabilizes new varieties without even using agar.

Though agar is highly recommended for anyone starting out, and is still used constantly by most excellent growers here. Someone starting should be doing transfers, T1, maybe T2.

F1, F2, for noobs, not so much. That's better after you've mastered the basics.

--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (03/20/23 05:04 AM)

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