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OfflineMycoSnailCarl
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Registered: 11/22/23
Posts: 181
Last seen: 1 day, 3 hours
Re: The OFFICIAL Stasis Storage Thread [Re: SeventhMushroom]
    #28612307 - 01/06/24 08:34 PM (21 days, 4 hours ago)

So at this point do we think LC stored in vials is fine? I’m thinking that it’d be easier/less likely to contaminate if using a vial or LC rather than trying to fish a piece of agar out of water.

Basically, which would be better: putting a small piece of agar in sterile water, or in LC solution?


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InvisiblePhaethon
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Registered: 07/04/22
Posts: 18
Re: The OFFICIAL Stasis Storage Thread [Re: 3.A.M]
    #28614679 - 01/08/24 11:19 PM (19 days, 1 hour ago)

Quote:

3.A.M said:
And if anyone’s interested I’ve been using small autoclave pouches with great success for storing and drying grains, also use them for my swabs, they’re sanitary and literally designed to wick away moisture and dry out while at the same time maintaining a protective shield against contamination, I just pour grains straight from the jar in them when I do my g2g process, seal them and let them dry from inside the pouch, super cheap and almost too easy.




Did you find autoclave pouches that come sterile? Or are you sterilizing the empty pouches first, or just taking a chance since you'll be reviving it on agar anyway and can transfer away from any contaminant that survives dessication?

fahster has a custom Tyvek lid design which I am considering making without any silicone (I would just use them for drying, preferring inoculation without a needle). But if I could dry and store in a single cheap, lightweight, and small container, that would be pretty great.


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LAGM2.023


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Offline3.A.M
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Registered: 10/17/22
Posts: 847
Loc: Oz
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Re: The OFFICIAL Stasis Storage Thread [Re: Phaethon]
    #28614720 - 01/09/24 01:18 AM (18 days, 23 hours ago)

They’re absolutely not sterile and a couple times I’ve had to transfer away from contam but for ease and practicality they’re amazing, and no I don’t sterilise first but you could easily throw some in a jar and put them through a cycle if you like.
I originally got them as an alternative to wrapping home sterilised swabs in foil which isn’t sterile either so grains were just the next natural step, fahts methods are definitely tried, tested and guaranteed successful though if you want to avoid potential contam.


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InvisiblePhaethon
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Registered: 07/04/22
Posts: 18
Re: The OFFICIAL Stasis Storage Thread [Re: 3.A.M]
    #28615920 - 01/10/24 02:06 AM (17 days, 22 hours ago)

Cool, thank you! I think I will try that, probably starting by sterilizing the pouches (if that doesn't do something awful to the seal tape).


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LAGM2.023


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Offline3.A.M
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Re: The OFFICIAL Stasis Storage Thread [Re: Phaethon]
    #28615940 - 01/10/24 03:08 AM (17 days, 21 hours ago)

Literally got some in the pc right now, glad we had this discussion!
I don’t use the seal tape anyway, did the first few times but it’s not really designed to come back off so I just masking tape it closed like most people do with swabs.
I’ll let you know if sterilisation warps the pouches at all but I don’t imagine it will, I’ve been using autoclaves for years in various industries, those pouches are pretty hardy.
You can also get roles of the stuff and heat seal them to any length you like if you want to go that avenue as well👍

Edit: pouches pc’d fine, they were a bit loose in the jar so they sagged down a little and will probably hold that shape but won’t effect function at all (:


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Edited by 3.A.M (01/10/24 02:10 PM)


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InvisiblePhaethon
Stranger
Registered: 07/04/22
Posts: 18
Re: The OFFICIAL Stasis Storage Thread [Re: 3.A.M]
    #28618006 - 01/11/24 07:26 PM (16 days, 5 hours ago)

Nice, thanks for the update!

I've got a couple different pouch sizes in my Amazon cart, along with some bulk desiccant to speed up drying. Will post an update once I've tried it.

Thanks again for the idea! (It's funny - when I first got into the hobby, I was looking for something to enclose things in while pressure cooking, other than foil or rigid containers. I'd seen "autoclave bags" mentioned, and found those almost entirely for the purpose of sterilizing before disposal rather than use. Sterilization pouches and rolls are what I was looking for! I've used polypropylene tubing to enclose stacks of PP petri dishes, but they come out quite wet and stay that way. I'm going to try stacking them in 7.5" x 13" pouches.)


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LAGM2.023


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