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Invisibleohmatic
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storage of liquid cultures / mycelium syringes in fridge
    #4094265 - 04/25/05 08:05 AM (11 years, 7 months ago)

ey!

how long should one be able to store a liquid culture in a fride?

i know that spore syringes can be stored for up to a year and even
longer and were reported to be viable, but since the liquid culture
is ALIVE i wonder how it would be like with it.

ofcoz the jar would have a air filter ..

also, how long should one be able to store a mycelium syringe in the fridge?
peace ohm :mushroom2:


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Edited by ohmatic (04/25/05 08:06 AM)


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OfflineIGnosticAbhorI
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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094272 - 04/25/05 08:09 AM (11 years, 7 months ago)

Cold will slow growth...So it'll have food for longer.....I think the longest i heard was 4 months....I wouldn't go past six....IMO

-Gnostic


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: IGnosticAbhorI]
    #4094273 - 04/25/05 08:10 AM (11 years, 7 months ago)

Quote:

IGnosticAbhorI said:
Cold will slow growth...So it'll have food for longer.....I think the longest i heard was 4 months....I wouldn't go past six....IMO

-Gnostic




is this talking for the jar WITH the filter or for the syringes?
syringe -> no gas 'exchange'
peace ohm :mushroom2:


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OfflineIGnosticAbhorI
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Re: storage of liquid cultures / mycelium syringes in fridge [Re: IGnosticAbhorI]
    #4094283 - 04/25/05 08:23 AM (11 years, 7 months ago)

I was talking about myc.(lc's)jars.....I heard of prints lasting for as long as 13 (17?) years, and syringes for up to a year....


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: IGnosticAbhorI]
    #4094292 - 04/25/05 08:31 AM (11 years, 7 months ago)

I have a syringe over 3 years old that is still viable, has been at room temp for the last 2 years.
I don't bother with gas exchange for my liquid innocs. If you shake up the solution after it should be aerated enough. During boiling amost of the oxygen is driven out of the liquid.

I have heard mycelium is meant to last for years if in distilled water. Need a way to separate it though. I once tried putting a piece of mushroom in sterile water with the intention of adding sugars to it at a later date to make a cloned liquid innoc. Didn't work though (for me)


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: IGnosticAbhorI]
    #4094299 - 04/25/05 08:51 AM (11 years, 7 months ago)

Yea, distilled lasts for a llloooonnngggg time....Myc. should work in distilled water...(like broken off a pf cake....It puts it in statis....

-Gnostic


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: IGnosticAbhorI]
    #4094314 - 04/25/05 09:12 AM (11 years, 7 months ago)

but given that the mycelium is actually in a nute sollution -> liquid inoculant,
the syringe in the fride would make it grow SLOWER but it would still grow -> co2 ???
peace ohm :mushroom2:


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RIP #cultivation! ....can't associate? well FUCK U !


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094320 - 04/25/05 09:19 AM (11 years, 7 months ago)

It would make co2, but at a very slow rate...

You'll notice it'll barely grow within a month at such cold temps...

-Gnostic


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: IGnosticAbhorI]
    #4094321 - 04/25/05 09:21 AM (11 years, 7 months ago)

Quote:

IGnosticAbhorI said:
It would make co2, but at a very slow rate...

You'll notice it'll barely grow within a month at such cold temps...

-Gnostic




well i guess as it is with most of these questions ill simply have to make couple of
syringes and see myself how long they actually last ..
peace ohm :mushroom2:


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094325 - 04/25/05 09:22 AM (11 years, 7 months ago)

After it is grown, the sugars are used up so it is basically in a mixture of distilled water and unused nutrients, but those nutrients might grow contams if any get in.

I wonder if it has a better chance if you suck in a small amount from the jar and squirt it in a second jar of distilled water and a little H2O2, thereby diluting the nutrients


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: blackout]
    #4094328 - 04/25/05 09:26 AM (11 years, 7 months ago)

Quote:

blackout said:
After it is grown, the sugars are used up so it is basically in a mixture of distilled water and unused nutrients, but those nutrients might grow contams if any get in.

I wonder if it has a better chance if you suck in a small amount from the jar and squirt it in a second jar of distilled water and a little H2O2, thereby diluting the nutrients




say for example, have a 1000ml jar, with 200ml of throughout colonised liquid media,
pour in additional 700ml of sterile water to bring to 900ml in total,
add some h2o2, flip on magnetic stirrer and suck up that liquid?
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleBrotherJohn
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Re: storage of liquid cultures / mycelium syringes in fridge [Re: blackout]
    #4094335 - 04/25/05 09:28 AM (11 years, 7 months ago)

Quote:

blackout said:
I wonder if it has a better chance if you suck in a small amount from the jar and squirt it in a second jar of distilled water and a little H2O2, thereby diluting the nutrients




What i always do is once the Myc is going strong(after about 1 week) I take an empty sterile syring and inject a feww CCs of H2O2 into my liquid Myc jar.This helps to battle the nasties if they wanna come into play later on.You just wanna make sure that the spores you injected have all germinated because although the peroxide wont hurt the myc,spores wont be able to germinate.


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094355 - 04/25/05 09:42 AM (11 years, 7 months ago)

Quote:

ohmatic said:
say for example, have a 1000ml jar, with 200ml of throughout colonised liquid media,
pour in additional 700ml of sterile water to bring to 900ml in total,
add some h2o2, flip on magnetic stirrer and suck up that liquid?
peace ohm :mushroom2:



I use mainly 300-400ml jam jars with a hole and a blob of silicon for a self healing patch, no air filters, lid always full tight. I was thinking you could syringe in a tiny bit of mycelium water, say 1-2ml and inject into a freshly prepared jar of just 200ml water. Then inject in some H2O2. If you are careful you can move around the jar and suck the small bit of mycelium back out. Otherwise you could inject a concentrated liquid innoculant, say 40% sugar, at a later date and revive the jar fully.

Yours would also work but I don't have room for a load of 1 litre jars in my fridge!


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Edited by blackout (04/25/05 09:43 AM)


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094423 - 04/25/05 10:19 AM (11 years, 7 months ago)


Culture started 2/11. Pic taken 4/25 (you do the math)
Stored @ 65F in total darkness.
The lid has a gas exchange filter.
Perfectly healthy & uncontaminated.
Without a gas exchange filter, this culture would be DEAD.


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: agar]
    #4094430 - 04/25/05 10:23 AM (11 years, 7 months ago)

Quote:

agar said:

Culture started 2/11. Pic taken 4/25 (you do the math)
Stored @ 65F in total darkness.
The lid has a gas exchange filter.
Perfectly healthy & uncontaminated.
Without a gas exchange filter, this culture would be DEAD.




lovely thats what ive been asking for.

now, without gas exchange, its CLEAR that it would be dead b/c it would eat up all
the avaible air and then, well, suffocate ..

what about mycelium syringes - they dont have any air exchange,
how long should they survive in the fridge?
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094455 - 04/25/05 10:36 AM (11 years, 7 months ago)

I would guess MAX about 4 months, if (big IF)the temp was a steady 40F & in total darkness. I have kept a few 2 months, not longer.

For longer term storage I take clean myc isolate off an agar petri, asperate into a syringe of sterile distilled water & inject it into a 10ml vacutainer tube. Those will last for YEARS, as there is no nutes, or air. Myc goes into suspended animation.


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: agar]
    #4094476 - 04/25/05 10:42 AM (11 years, 7 months ago)

Quote:

agar said:
I would guess MAX about 4 months, if (big IF)the temp was a steady 40F & in total darkness. I have kept a few 2 months, not longer.

For longer term storage I take clean myc isolate off an agar petri, asperate into a syringe of sterile distilled water & inject it into a 10ml vacutainer tube. Those will last for YEARS, as there is no nutes, or air. Myc goes into suspended animation.




allright :smile:

so, if i had one of these lovely eberbach blenders and simply blended a piece of agar
in sterile water and sucked that up for syringes, those syringes should be able
to be stores in the fridge for an indefinite ammount of time as the
mycelium would simply be in a NUTELESS sterile water environment ..

basically the same as with your vacutainers !

riiiiiiiiiight?
peace ohm :mushroom2:

*edit*
it just hit me that the so called 9er tek is like a "homemade eberbach blender"
it should work just as good using that baby aiiight?


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RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (04/25/05 10:44 AM)


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094486 - 04/25/05 10:44 AM (11 years, 7 months ago)

Colonized agar has nutes, otherwise myc would not colonize it. So, you would have nutes in the solution. Which is not the same as I do.


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: agar]
    #4094489 - 04/25/05 10:45 AM (11 years, 7 months ago)

Quote:

agar said:
Colonized agar has nutes, otherwise myc would not colonize it. So, you would have nutes in the solution. Which is not the same as I do.




damn right :/
sometimes i think a little bit too far .. damn :frown:
peace ohm :mushroom2:


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RIP #cultivation! ....can't associate? well FUCK U !


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Re: storage of liquid cultures / mycelium syringes in fridge [Re: ohmatic]
    #4094535 - 04/25/05 11:05 AM (11 years, 7 months ago)

But should a piece of mushroom tissue in water be ok?
I tried this and thinking back I think I tried to fish it out and put it on an agar plate. A better way is to add some sterile concentrated sugar solution as I mentioned before.


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Edited by blackout (04/25/05 11:05 AM)


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