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OfflineDredgeMyEyes
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Registered: 11/05/14
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28360491 - 06/15/23 06:43 AM (7 months, 9 days ago)



What do you guys think about packing a microfuge tube with  boiled hydrated wood pieces, wrapped in foil and sterilized in a jar with a sfd and then innocoulated with a few drops of known to be good LC or tiny sample from  a plate for long term storage?

Pull out a twig and toss it on a plate to revive

Gonna try it


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OfflineScrewup
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Re: The OFFICIAL Stasis Storage Thread [Re: DredgeMyEyes]
    #28360500 - 06/15/23 06:48 AM (7 months, 9 days ago)

Why can’t I use filtered or bottled water? Buying a gallon of distilled is so wasteful.


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OfflineDredgeMyEyes
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Re: The OFFICIAL Stasis Storage Thread [Re: Screwup]
    #28360503 - 06/15/23 06:51 AM (7 months, 9 days ago)

I'm fairly certain you can use tap water without issue


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OfflineScrewup
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Re: The OFFICIAL Stasis Storage Thread [Re: DredgeMyEyes]
    #28360505 - 06/15/23 06:52 AM (7 months, 9 days ago)

Quote:

DredgeMyEyes said:
I'm fairly certain you can use tap water without issue



WELL FUCK


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OfflineDredgeMyEyes
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Registered: 11/05/14
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Re: The OFFICIAL Stasis Storage Thread [Re: Screwup]
    #28360509 - 06/15/23 07:03 AM (7 months, 9 days ago)

Probably not 100% ideal just cause there are some dissolved minerals and other stuff but it's fine for agar and LC and all.

Alternatively you could gather some distilled water at home  if you look around on the internet there are some methods that involve boiling water in a pot and collecting the condensate in a smaller vessel sitting in the center of the pot I believe.

Someone else will hopefully chime in as I'm no expert lol


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Offlinefiddle_headS
I'm not the dude, guy
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28360511 - 06/15/23 07:07 AM (7 months, 9 days ago)

I’ve been reading on cryovials and centrifuge tubes, think I’m gonna give it a try for my next project. Bout time I got some of these winners in the Rolodex
Thanks Donny 🀘


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Offlinefossilshark
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Registered: 08/05/20
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Re: The OFFICIAL Stasis Storage Thread [Re: Screwup] * 1
    #28360775 - 06/15/23 12:15 PM (7 months, 9 days ago)

I use bottled water just because its what I have on hand.

As for wooden pieces I am finding that putting boiled and sterilized pieces of wood skewers does work but takes a long time for myc to colonize it. I do not know how well it would work just putting a wedge or a bit of LC on it.


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LITFA LITFA LITFA


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Offlinebongoman
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28366314 - 06/19/23 09:12 PM (7 months, 5 days ago)

Let's say I have some spore prints on hand that I don't intend to grow out in the new future.

Would it make sense to start up these spore prints on agar just to get some clean growth to then store using this method?

Would it better to preserve genetics using an agar wedge in water or as a spore print?


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OfflineUnikern13
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Registered: 05/11/23
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Re: The OFFICIAL Stasis Storage Thread [Re: bongoman]
    #28371733 - 06/23/23 07:26 PM (7 months, 1 day ago)

Would plucking invitro pins and dropping them into sterile water tubes work for stasis storage? If no one knows, I’m gonna find out sooner or later because that’s what I ended up doing, but if anyone has any experience that would save me time that would be awesome.


Edited by Unikern13 (06/29/23 11:00 AM)


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Offlinefossilshark
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark] * 3
    #28405356 - 07/23/23 03:55 PM (6 months, 2 days ago)

Threw some Gymnopilus Purperatus and Gandalf in stasis today, decided to switch over to wood sticks as they are way easier to handle then paper.

I wonder how necessary the water even is?





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Offlineastra
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark] * 3
    #28410278 - 07/27/23 11:43 AM (5 months, 29 days ago)

dHβ‚‚O 2ml cryovials






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:ancientaliens:


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OfflineLeche de
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Registered: 09/05/22
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Re: The OFFICIAL Stasis Storage Thread [Re: DredgeMyEyes]
    #28410295 - 07/27/23 11:52 AM (5 months, 29 days ago)

Hesitant on the lc part- but it’s a neat idea


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He need some milk


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Offlineside-eye
potate


Registered: 09/01/22
Posts: 55
Last seen: 1 month, 23 days
Re: The OFFICIAL Stasis Storage Thread [Re: bongoman]
    #28419032 - 08/03/23 04:48 PM (5 months, 22 days ago)

Quote:

bongoman said:
Let's say I have some spore prints on hand that I don't intend to grow out in the new future.

Would it make sense to start up these spore prints on agar just to get some clean growth to then store using this method?

Would it better to preserve genetics using an agar wedge in water or as a spore print?




That's what I'm doing with all of my spore prints/swabs now. I don't plan on growing for a while, but I'm getting everything germed and put in stasis. Not only is it a great secondary backup, but I think it'll help to speed things up when I'm ready to grow again.


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LAGM 2023


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Offlineside-eye
potate


Registered: 09/01/22
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28419036 - 08/03/23 04:50 PM (5 months, 22 days ago)

Quote:

fossilshark said:
Threw some Gymnopilus Purperatus and Gandalf in stasis today, decided to switch over to wood sticks as they are way easier to handle then paper.

I wonder how necessary the water even is?








How did you like this compared to paper, other than handling? Guess we won't know til revived. Any issue with the sticks floating when you drop em in?


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LAGM 2023


Edited by side-eye (08/03/23 04:57 PM)


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Offlinebongoman
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Re: The OFFICIAL Stasis Storage Thread [Re: side-eye]
    #28426261 - 08/08/23 08:15 PM (5 months, 17 days ago)

Why use paper etc when the original research study at https://sci-hub.st/10.1139/w08-049 uses 8mm agar punches?

It seems way simpler to just drop a wedge to sterile water no?


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InvisibleBigwormS
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Registered: 03/19/22
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Loc: Murica
Re: The OFFICIAL Stasis Storage Thread [Re: bongoman] * 2
    #28426269 - 08/08/23 08:22 PM (5 months, 17 days ago)

Cryo storage works well. I can't say I would trust anything but distilled water with my cultures but I'm sure it's worth a try. I also made mini slants with boiled toothpicks and agar for a shorter storage method compared to the distilled cryo storage.


Edited by Bigworm (08/08/23 08:22 PM)


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OfflineKHRIST0PH0R0US
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Registered: 08/23/17
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28440429 - 08/20/23 10:19 AM (5 months, 5 days ago)

I was just thinking about this the other day. Advanced methods of storage or even breeding/selecting somehow for hardiness in long term storage. You said
"In theory this can lead to changes in the culture that is not desired." - is that covered in the paper you linked to or can you elaborate a little as to what changes or does it just stop at "in theory"? May it be truly random changes that could affect any aspects of it's makeup and manifest itself in an infinite number of ways?


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Offlinerizingfire
Mycoticus psychoticus
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Re: The OFFICIAL Stasis Storage Thread [Re: KHRIST0PH0R0US]
    #28457378 - 09/03/23 04:21 PM (4 months, 22 days ago)

Wish I would have known about this back in the day, literally lost gwm iso b cuz the 13yr old print was completely dead but an older version of the original was able to be brought back by Murderlabz so we made iso d from it...tho now we have a nat cross so the new gwmn will be a hybrid. I will save my stuff using cold stasis from now on...way easier


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aka NHMI


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OfflineScrewup
Googles your dumb questions
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Registered: 01/27/22
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Re: The OFFICIAL Stasis Storage Thread [Re: rizingfire]
    #28457381 - 09/03/23 04:22 PM (4 months, 22 days ago)

Are you just doing marketing for that company lol?


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InvisiblefahtsterM
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Re: The OFFICIAL Stasis Storage Thread [Re: Screwup] * 3
    #28486326 - 09/29/23 12:01 AM (3 months, 28 days ago)

Getting to be about that time of year for me.  Started reviving some dried out grain cultures 8 days ago.  I GLC the dry grains and inject them into fresh grains.  I can (and sometimes do) go straight to qts but I like to test the cultures first in small jars just to be sure. 

Claketi (dried out April 2023)



PE x RW f7 clone (dried out March 2023)



Mak TP (this one is from March 2021)



Fp+ f8 clone (dried out April 2023)



Shit amazes me every time.  These new jars will be used to glc to qts for tubs.  When I made these new jars, I made a second one of each that will be dried out again for further storage


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