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Offline174BPM
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Registered: 05/12/19
Posts: 309
Last seen: 7 months, 24 days
Re: The OFFICIAL Stasis Storage Thread [Re: mycodependent]
    #28248914 - 03/27/23 08:32 AM (9 months, 27 days ago)

:like:


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Offlineastra
Mycelia-Of-House-Fungi
Female


Registered: 12/24/21
Posts: 81
Loc: Japan Flag
Last seen: 4 hours, 17 minutes
Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28254070 - 03/30/23 04:25 AM (9 months, 24 days ago)

:threadmonitor:


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:ancientaliens:


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Invisibledna24
Darth Randal
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Registered: 04/19/22
Posts: 360
Loc: Savages
Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark] * 1
    #28254230 - 03/30/23 07:53 AM (9 months, 24 days ago)

Quote:

fossilshark said:
Spores are just gametes and not fully developed cells so they wouldnt have the resources to completely shut down and stay viable.

Based on what methods people use at the moment if you get rid of just one of the three oxygen, water, or nutrients the mycelium will go into stasis and be viable until it is either destroyed or wakes up.

Stasis storage of mycelium cultures needs no overcomplicating.



THIS, this is the way.


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:flipthebird::stonedjerk: FUCK STIPE :stonedjerk::flipthebird:


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Offlinerxb
n00b-sabot
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Registered: 08/24/13
Posts: 9,482
Loc: FREE PSYCHONAUTICA
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Re: The OFFICIAL Stasis Storage Thread [Re: dna24]
    #28291407 - 04/23/23 01:02 PM (9 months, 12 hours ago)

i think im going to try using sterile swabs.

they are cheap, they are already sterile, they will soak up an lc.

soak as many as you like in clean(ish) lc, then put them into a vial of DI water. ship anywhere...save 500 an hour if you want to do the work, and potentially legally sell and ship as much as you like (not for me but maybe for you)

i think its easier than paper, and i think its faster to soak up lc than a skewer, i dont think the cotton is enough of a nutrient to be an issue, the only foiling factor might be if a swab didnt get any tissue on it, so run that stir bar at warp5.

this thread is a great inspiration!!! thank you :smile:


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->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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OfflineSoFlown99
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Registered: 02/25/23
Posts: 5
Last seen: 7 months, 13 days
Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark] * 1
    #28299254 - 04/28/23 07:24 PM (8 months, 26 days ago)

Stasis has been achieved :mushroom2:

Thank you for this wonderful writeup and all your help on stasis!



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Offlineside-eye
potate


Registered: 09/01/22
Posts: 55
Last seen: 1 month, 23 days
Re: The OFFICIAL Stasis Storage Thread [Re: SoFlown99] * 1
    #28299273 - 04/28/23 07:39 PM (8 months, 26 days ago)

Put a few more cultures into stasis today. PC'd 18 of these tubes, cut some long wedges, and dropped em in. Wrap, label, into the rack.



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LAGM 2023


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OfflineSporeJockey
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Registered: 05/28/23
Posts: 6
Last seen: 7 months, 3 days
Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28337682 - 05/28/23 12:08 AM (7 months, 28 days ago)

Just what I needed, this the one. ^^^


Edited by SporeJockey (05/28/23 12:20 AM)


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InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: The OFFICIAL Stasis Storage Thread [Re: SporeJockey] * 1
    #28337684 - 05/28/23 12:09 AM (7 months, 28 days ago)

Quote:

SporeJockey said:
.




Excellent first post.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


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OfflineSporeJockey
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Registered: 05/28/23
Posts: 6
Last seen: 7 months, 3 days
Re: The OFFICIAL Stasis Storage Thread [Re: Way] * 1
    #28337690 - 05/28/23 12:19 AM (7 months, 28 days ago)

Lmao, I wanted to save the post but didn’t know how. So at least I can access my threads. 😂


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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,293
Last seen: 7 hours, 5 minutes
Re: The OFFICIAL Stasis Storage Thread [Re: SporeJockey] * 1
    #28337750 - 05/28/23 02:39 AM (7 months, 27 days ago)

Quote:

SporeJockey said:
Lmao, I wanted to save the post but didn’t know how. So at least I can access my threads. 😂



Do people have zero idea how to navigate on browsers for bookmarks/favorites? Y’all kill me.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineSporeJockey
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Registered: 05/28/23
Posts: 6
Last seen: 7 months, 3 days
Re: The OFFICIAL Stasis Storage Thread [Re: Screwup]
    #28338354 - 05/28/23 02:48 PM (7 months, 27 days ago)

Save within the website, not bookmarks…


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OfflineRushing
Stranger


Registered: 01/09/23
Posts: 10
Last seen: 14 days, 21 hours
Re: The OFFICIAL Stasis Storage Thread [Re: SporeJockey] * 1
    #28354239 - 06/10/23 08:57 AM (7 months, 14 days ago)

Started putting some culture into the stasis library







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Offlinefossilshark
DouchebagDonny
I'm a teapot User Gallery


Registered: 08/05/20
Posts: 132
Last seen: 4 days, 7 hours
Re: The OFFICIAL Stasis Storage Thread [Re: Rushing] * 1
    #28355329 - 06/11/23 08:30 AM (7 months, 13 days ago)

Love seeing everyone's take on stasis storage! Thats exactly why I created this thread, would love to see more!


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LITFA LITFA LITFA


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OfflineSporeJockey
Stranger

Registered: 05/28/23
Posts: 6
Last seen: 7 months, 3 days
Re: The OFFICIAL Stasis Storage Thread [Re: Rushing]
    #28355994 - 06/11/23 06:16 PM (7 months, 13 days ago)

Could you send a link for those bottles in the last pic? What’s the measurement on them??


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OfflineSporeJockey
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Registered: 05/28/23
Posts: 6
Last seen: 7 months, 3 days
Re: The OFFICIAL Stasis Storage Thread [Re: side-eye]
    #28356004 - 06/11/23 06:18 PM (7 months, 13 days ago)

Would it actually go into stasis though since it has more agar to feed on??


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OfflineSporeJockey
Stranger

Registered: 05/28/23
Posts: 6
Last seen: 7 months, 3 days
Re: The OFFICIAL Stasis Storage Thread [Re: Guerrilla]
    #28356034 - 06/11/23 06:30 PM (7 months, 13 days ago)

Would that not effectively turn the whole shebang into an LC because of the nutes in the agar?


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InvisibleUltravisit0r
apprentice
Male


Registered: 04/03/23
Posts: 55
Re: The OFFICIAL Stasis Storage Thread [Re: SporeJockey] * 1
    #28357907 - 06/13/23 03:57 AM (7 months, 11 days ago)

Want do you think:
Make transfers to water agar plates (no nutes), then transfer to distilled water.

Is that a viable option theoreticaly? Am I missing something?


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OfflineDredgeMyEyes
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Registered: 11/05/14
Posts: 273
Last seen: 28 days, 4 hours
Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28357927 - 06/13/23 04:50 AM (7 months, 11 days ago)

what's the revival procedure like as far as taking a sample from the vial of distilled water? do you sort of fish it out with tweezers?  pin it to side with a scalpel and slide it to the top and remove it? pour out the water completely then remove? I'm probably over thinking it but just would like to know how this part goes lol

Does the water itself become myceliated and can be used to get a plate going?

Best tool and method for each

-Wood sticks
-Paper
-Wedges


Edited by DredgeMyEyes (06/13/23 04:54 AM)


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InvisibleUltravisit0r
apprentice
Male


Registered: 04/03/23
Posts: 55
Re: The OFFICIAL Stasis Storage Thread [Re: DredgeMyEyes]
    #28357954 - 06/13/23 05:32 AM (7 months, 11 days ago)

I'd drain the little container and poke little pieces of agar with a scalpel and put it on a new plate. Same thing with paper I guess


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Offlinefossilshark
DouchebagDonny
I'm a teapot User Gallery


Registered: 08/05/20
Posts: 132
Last seen: 4 days, 7 hours
Re: The OFFICIAL Stasis Storage Thread [Re: DredgeMyEyes] * 1
    #28358195 - 06/13/23 10:01 AM (7 months, 11 days ago)

The medium you use to get myc into your stasis vials makes no difference in the long run. Choose something that you feel comfortable handling.

As far as too much nuts on a medium like an agar wedge, even if the vial does become LC at some point it will run out of nuts and go into stasis. The only concern with this is possibility of it becoming too thick to get out easily but i doubt that would be a concern with just a slice of agar.


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LITFA LITFA LITFA


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