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Offlinefossilshark
DouchebagDonny


Reged: 08/05/20
Posts: 132
The OFFICIAL Stasis Storage Thread
      01/28/23 12:06 PM

I've been waiting a long time to make this thread as this is something I am very interested in. The goal of this thread is to share the many different methods of storing cultures in stasis. I will be posting my method and I would love for everyone to contribute their method of storing cultures in stasis. I in NO way came up with stasis storage and it has been around in many different forms for decades.

What is stasis storage?
Stasis storage is storing a sterile sample of mycelium in a no-oxygen no-nutrient environment causing the mycelium to go into stasis ceasing all metabolic activity.

Why stasis storage?
There are multiple advantages of stasis storage over other long term culture storage methods.

#1: The biggest advantage of stasis storage is the complete inaction of metabolic activity. Other long term culture storage methods (slants, cryo) only slow the metabolic activity down. In theory this can lead to changes in the culture that is not desired. Furthermore this means that unlike slants you do not have to "wake up" the mycelium at any point to continue to preserve it. Stasis has been shown to be effective for 30 years in https://sci-hub.st/10.1139/w08-049 and thats just when they stopped the study. In theory stasis storage is indefinite.

#2: Stasis storage is super simple and can be done with solid media or liquid or just straight up agar wedges. Very universal technique for anyone's style.

#3: My favorite part, stasis storage works at room temperature. Absolutely no need to take up valuable space in your moms fridge. :wink:

My method:

My initial trials 2 years ago were done to preserve Enigma x Rusty Whyte F2 from Solipsis using paper strips. I plan on switching over to wood skewers to make things easier to handle. First I hydrated paper strips by dunking paper strips in distilled water for a few seconds and sterilizing them for 20 minutes in my trusty presto along with some vials of distilled water.

After the paper strips and water were prepped I laid the strips on colonized germ plates (more strains more better baby) and let the mycelium colonize them for about a week.



After the paper strips were colonized I transferred them into vials of distilled water (along with some fat plate pins to try and clone them in the future).



And done. Thats the whole tek. Stasis storage is stupid simple. I ran the cultures about a year after I made them and they bounced back very quickly and I plan on doing a 2 year test next month when I have time.

NOTES: While the original stasis vials I made are useable its apparent that my sterile technique back then was sub par. One of the vials had a scoby on top but the mycelium was just fine.

For coprophilic species paper strips, wood skewers, toothpicks ECT work just fine with no issues. I theorize that if you want to store lignophilic species this would be too much available nutrients and effectively make the solution into LC. This would still work as once LC runs out of nutrients it goes into stasis, but to me that does not seem ideal. A better solution would be to use SFD discs like in the original paper.

I appreciate any questions or discourse on this thread on the topic. I think this method is an amazing tool for a mycology enthusiast. I appreciate those of you who take the time to read through this post. :mushroom2:

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LITFA LITFA LITFA

Post Extras Print Post Remind Me! Notify Moderator
. * * Re: The OFFICIAL Stasis Storage Thread VietboyX   01/28/23 12:12 PM
. * * Re: The OFFICIAL Stasis Storage Thread side-eye   01/28/23 12:16 PM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   01/28/23 12:23 PM
. * * Re: The OFFICIAL Stasis Storage Thread Nextcontestant   01/28/23 12:34 PM
. * * Re: The OFFICIAL Stasis Storage Thread fahtsterT   01/28/23 03:33 PM
. * * Re: The OFFICIAL Stasis Storage Thread bakedbeings   01/28/23 03:45 PM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   01/28/23 05:17 PM
. * * Re: The OFFICIAL Stasis Storage Thread PastywhyteM   01/29/23 08:01 AM
. * * Re: The OFFICIAL Stasis Storage Thread bakedbeings   01/29/23 08:13 AM
. * * Re: The OFFICIAL Stasis Storage Thread fahtsterT   01/29/23 08:32 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   01/29/23 11:37 AM
. * * Re: The OFFICIAL Stasis Storage Thread fahtsterT   02/11/23 07:45 PM
. * * Re: The OFFICIAL Stasis Storage Thread side-eye   02/16/23 04:09 PM
. * * Re: The OFFICIAL Stasis Storage Thread Guerrilla   02/16/23 04:17 PM
. * * Re: The OFFICIAL Stasis Storage Thread Kabbal   02/22/23 05:15 PM
. * * Re: The OFFICIAL Stasis Storage Thread SporeJockey   06/11/23 06:30 PM
. * * Re: The OFFICIAL Stasis Storage Thread Ultravisit0r   06/13/23 03:57 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   01/29/23 11:36 AM
. * * Re: The OFFICIAL Stasis Storage Thread bakedbeings   01/29/23 11:44 AM
. * * Re: The OFFICIAL Stasis Storage Thread dna24   01/29/23 11:54 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   01/29/23 06:45 PM
. * * Re: The OFFICIAL Stasis Storage Thread fungusul   01/30/23 08:02 PM
. * * Re: The OFFICIAL Stasis Storage Thread Range Spacer   01/31/23 04:22 PM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   02/01/23 03:31 AM
. * * Re: The OFFICIAL Stasis Storage Thread Guerrilla   02/01/23 03:54 AM
. * * Re: The OFFICIAL Stasis Storage Thread AyePlus   02/01/23 06:00 AM
. * * Re: The OFFICIAL Stasis Storage Thread HILLBILLY OUTLAW   01/31/23 04:37 PM
. * * Re: The OFFICIAL Stasis Storage Thread LadysKnight   02/01/23 06:03 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   02/01/23 08:24 AM
. * * Re: The OFFICIAL Stasis Storage Thread towndaze   02/02/23 06:33 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   02/02/23 07:30 PM
. * * Re: The OFFICIAL Stasis Storage Thread towndaze   02/05/23 10:35 PM
. * * Re: The OFFICIAL Stasis Storage Thread Sulfurshelfsean   02/06/23 05:09 AM
. * * Re: The OFFICIAL Stasis Storage Thread Hindsight   02/06/23 09:19 AM
. * * Re: The OFFICIAL Stasis Storage Thread bakedbeings   02/06/23 10:29 AM
. * * Re: The OFFICIAL Stasis Storage Thread side-eye   02/11/23 08:50 AM
. * * Re: The OFFICIAL Stasis Storage Thread nektar61   02/11/23 07:32 PM
. * * Re: The OFFICIAL Stasis Storage Thread nektar61   02/05/23 07:50 PM
. * * Re: The OFFICIAL Stasis Storage Thread B Traven   02/05/23 08:32 PM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   02/05/23 09:53 PM
. * * Re: The OFFICIAL Stasis Storage Thread dna24   03/30/23 07:53 AM
. * * Re: The OFFICIAL Stasis Storage Thread rxb   04/23/23 01:02 PM
. * * Re: The OFFICIAL Stasis Storage Thread mycodependent   03/08/23 05:08 PM
. * * Re: The OFFICIAL Stasis Storage Thread 174BPM   03/27/23 08:32 AM
. * * Re: The OFFICIAL Stasis Storage Thread astra   03/30/23 04:25 AM
. * * Re: The OFFICIAL Stasis Storage Thread SoFlown99   04/28/23 07:24 PM
. * * Re: The OFFICIAL Stasis Storage Thread side-eye   04/28/23 07:39 PM
. * * Re: The OFFICIAL Stasis Storage Thread SporeJockey   06/11/23 06:18 PM
. * * Re: The OFFICIAL Stasis Storage Thread SporeJockey   05/28/23 12:08 AM
. * * Re: The OFFICIAL Stasis Storage Thread Way   05/28/23 12:09 AM
. * * Re: The OFFICIAL Stasis Storage Thread SporeJockey   05/28/23 12:19 AM
. * * Re: The OFFICIAL Stasis Storage Thread Screwup   05/28/23 02:39 AM
. * * Re: The OFFICIAL Stasis Storage Thread SporeJockey   05/28/23 02:48 PM
. * * Re: The OFFICIAL Stasis Storage Thread Rushing   06/10/23 08:57 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   06/11/23 08:30 AM
. * * Re: The OFFICIAL Stasis Storage Thread DredgeMyEyes   06/13/23 04:50 AM
. * * Re: The OFFICIAL Stasis Storage Thread Ultravisit0r   06/13/23 05:32 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   06/13/23 10:01 AM
. * * Re: The OFFICIAL Stasis Storage Thread DredgeMyEyes   06/15/23 06:43 AM
. * * Re: The OFFICIAL Stasis Storage Thread Screwup   06/15/23 06:48 AM
. * * Re: The OFFICIAL Stasis Storage Thread DredgeMyEyes   06/15/23 06:51 AM
. * * Re: The OFFICIAL Stasis Storage Thread Screwup   06/15/23 06:52 AM
. * * Re: The OFFICIAL Stasis Storage Thread DredgeMyEyes   06/15/23 07:03 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   06/15/23 12:15 PM
. * * Re: The OFFICIAL Stasis Storage Thread bongoman   06/19/23 09:12 PM
. * * Re: The OFFICIAL Stasis Storage Thread Unikern13   06/23/23 07:26 PM
. * * Re: The OFFICIAL Stasis Storage Thread side-eye   08/03/23 04:48 PM
. * * Re: The OFFICIAL Stasis Storage Thread Leche de   07/27/23 11:52 AM
. * * Re: The OFFICIAL Stasis Storage Thread SporeJockey   06/11/23 06:16 PM
. * * Re: The OFFICIAL Stasis Storage Thread fiddle_head   06/15/23 07:07 AM
. * * Re: The OFFICIAL Stasis Storage Thread fossilshark   07/23/23 03:55 PM
. * * Re: The OFFICIAL Stasis Storage Thread side-eye   08/03/23 04:50 PM
. * * Re: The OFFICIAL Stasis Storage Thread bongoman   08/08/23 08:15 PM
. * * Re: The OFFICIAL Stasis Storage Thread Bigworm   08/08/23 08:22 PM
. * * Re: The OFFICIAL Stasis Storage Thread astra   07/27/23 11:43 AM
. * * Re: The OFFICIAL Stasis Storage Thread KHRIST0PH0R0US   08/20/23 10:19 AM
. * * Re: The OFFICIAL Stasis Storage Thread rizingfire   09/03/23 04:21 PM
. * * Re: The OFFICIAL Stasis Storage Thread Screwup   09/03/23 04:22 PM
. * * Re: The OFFICIAL Stasis Storage Thread fahtsterT   09/29/23 12:01 AM
. * * Re: The OFFICIAL Stasis Storage Thread Mycomedicine888   10/10/23 12:27 PM
. * * Re: The OFFICIAL Stasis Storage Thread tree frog   10/12/23 07:37 AM
. * * Re: The OFFICIAL Stasis Storage Thread DERRAYLD   10/12/23 07:40 AM
. * * Re: The OFFICIAL Stasis Storage Thread tree frog   10/12/23 08:31 AM
. * * Re: The OFFICIAL Stasis Storage Thread soppos   10/17/23 04:16 PM
. * * Re: The OFFICIAL Stasis Storage Thread bongoman   10/18/23 04:15 PM
. * * Re: The OFFICIAL Stasis Storage Thread ReverendMyc   10/18/23 04:25 PM
. * * Re: The OFFICIAL Stasis Storage Thread bongoman   10/18/23 04:34 PM
. * * Re: The OFFICIAL Stasis Storage Thread Nichrome   10/18/23 06:47 PM
. * * Re: The OFFICIAL Stasis Storage Thread bongoman   10/21/23 07:39 PM
. * * Re: The OFFICIAL Stasis Storage Thread Mycolorado   10/21/23 07:41 PM
. * * Re: The OFFICIAL Stasis Storage Thread SerMantis   10/27/23 08:46 AM
. * * Re: The OFFICIAL Stasis Storage Thread Floret   12/03/23 12:24 AM
. * * Re: The OFFICIAL Stasis Storage Thread GuyOnaBuffalo   12/03/23 11:28 AM
. * * Re: The OFFICIAL Stasis Storage Thread c01h   12/16/23 06:34 PM
. * * Re: The OFFICIAL Stasis Storage Thread 3.A.M   01/01/24 05:28 AM
. * * Re: The OFFICIAL Stasis Storage Thread c01h   01/01/24 09:42 AM
. * * Re: The OFFICIAL Stasis Storage Thread 3.A.M   01/01/24 01:09 PM
. * * Re: The OFFICIAL Stasis Storage Thread the_chosen_one   01/04/24 04:40 PM
. * * Re: The OFFICIAL Stasis Storage Thread SeventhMushroom   01/04/24 06:23 PM
. * * Re: The OFFICIAL Stasis Storage Thread MycoSnailCarl   01/06/24 08:34 PM
. * * Re: The OFFICIAL Stasis Storage Thread Phaethon   01/08/24 11:19 PM
. * * Re: The OFFICIAL Stasis Storage Thread 3.A.M   01/09/24 01:18 AM
. * * Re: The OFFICIAL Stasis Storage Thread Phaethon   01/10/24 02:06 AM
. * * Re: The OFFICIAL Stasis Storage Thread 3.A.M   01/10/24 03:08 AM
. * * Re: The OFFICIAL Stasis Storage Thread Phaethon   01/11/24 07:26 PM


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