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InvisibleGuerrilla
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Registered: 01/30/21
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28167030 - 02/01/23 03:54 AM (11 months, 19 days ago)

:popcorn:


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InvisibleAyePlusS
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Re: The OFFICIAL Stasis Storage Thread [Re: Guerrilla]
    #28167111 - 02/01/23 06:00 AM (11 months, 19 days ago)

:solidnod:

Hate losing cold storage cultures. Definitely trying this out


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InvisibleLadysKnight
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28167113 - 02/01/23 06:03 AM (11 months, 19 days ago)

Quote:

fossilshark said:

After the paper strips and water were prepped I laid the strips on colonized germ plates (more strains more better baby) and let the mycelium colonize them for about a week.





Since spores are dirty, germ plates are dirty. I guess this is where you got the contam.


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Offlinefossilshark
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Registered: 08/05/20
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Re: The OFFICIAL Stasis Storage Thread [Re: LadysKnight]
    #28167217 - 02/01/23 08:24 AM (11 months, 19 days ago)

Probably lol. I definitely didnt lose the genetics tho as ive been able to revive them on 2 seperate occasions.


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Offlinetowndaze
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28168599 - 02/02/23 06:33 AM (11 months, 18 days ago)

Does that mean we finally have a mass-market ready Rusty Whyte & APE distribution technique?

Because shit son with this you could create [url=https://www.shroomery.org/forums/showflat.php/Number/27331873#27331873 ]giga mega super ultra isolated genetics across thousands of sheets of paper[/url] worth of completely standardized mycelium. Is it natural? Of course not. Is it useful? Quite likely.


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Time is nature's way of making sure that everything doesn't happen at once.


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Offlinefossilshark
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Re: The OFFICIAL Stasis Storage Thread [Re: towndaze]
    #28169710 - 02/02/23 07:30 PM (11 months, 18 days ago)

Not a clue what you just said but hell yes brother I love the energy.


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Offlinenektar61S
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28173923 - 02/05/23 07:50 PM (11 months, 15 days ago)

Fascinating stuff. Thank you.

I'm curious why this will grow and old spores won't seem to. I know someone revitalized a 20 plus year old print for Redboy, but I've seen people fail to grow spores that are under 5 years old. I believe stasis works but don't know why it does and storing spores seems to not work well.

Would removing all oxygen help? What about storing cultures without the water, and in argon or similar? I know that's complicating something simple, but I'm wondering if a tiny bit of oxygen is needed.

I looked up that arctic seed vault where Bill Gates stores his seeds to grow on all the farm land he's buying after he and his friends wreck the world (just kidding, maybe), this is the tek. It's seeds, not growing culture, and plants, not fungus, but interesting:

"The seeds are stored in sealed three-ply foil packages and then placed into plastic tote containers on metal shelving racks.[51] The storage rooms are kept at โˆ’18 ยฐC (โˆ’0.4 ยฐF). The low temperature and limited access to oxygen will ensure low metabolic activity and delay seed ageing. The permafrost surrounding the facility will help maintain the low temperature of the seeds if the electricity supply fails."

https://en.wikipedia.org/wiki/Svalbard_Global_Seed_Vault#Seed_storage



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OfflineB Traven
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Re: The OFFICIAL Stasis Storage Thread [Re: nektar61]
    #28173969 - 02/05/23 08:32 PM (11 months, 15 days ago)

:threadmonitor:


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Offlinefossilshark
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Re: The OFFICIAL Stasis Storage Thread [Re: nektar61] * 3
    #28174161 - 02/05/23 09:53 PM (11 months, 15 days ago)

Spores are just gametes and not fully developed cells so they wouldnt have the resources to completely shut down and stay viable.

Based on what methods people use at the moment if you get rid of just one of the three oxygen, water, or nutrients the mycelium will go into stasis and be viable until it is either destroyed or wakes up.

Stasis storage of mycelium cultures needs no overcomplicating.


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Offlinetowndaze
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28174227 - 02/05/23 10:35 PM (11 months, 15 days ago)

Quote:

fossilshark said:
Not a clue what you just said but hell yes brother I love the energy.



Sorry I was high. And now I'm high again. Let's break it down. Many more exotic variants don't or don't easily produce spores, which heavily restricts their spread. If you do a super isolated LC then freeze it onto paper you can create hundreds of hyper-standardized vials a day.


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OfflineSulfurshelfsean
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Re: The OFFICIAL Stasis Storage Thread [Re: towndaze]
    #28174393 - 02/06/23 05:09 AM (11 months, 14 days ago)

I've had lcs last years. One out out clean mycellium for six years but eventually they contam. I always use very little nute so I always wondered if they were in stasis. After about a year the culture liquid is completely clear. This looks like a much better way to store thank you for the write up. I'm going to try this.


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OfflineHindsight
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Re: The OFFICIAL Stasis Storage Thread [Re: Sulfurshelfsean] * 1
    #28174613 - 02/06/23 09:19 AM (11 months, 14 days ago)

Interesting idea. I do the same thing but just take a piece of colonized agar and put it in sterile distilled water vial. It has worked well for me. There is, of course, a small amount of nutrient from the agar, but it doesn't seem to impact storage life. Paper is a nutrient source so it seems to be a roughly equal approach.


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Invisiblebakedbeings
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Registered: 09/01/20
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Re: The OFFICIAL Stasis Storage Thread [Re: Sulfurshelfsean] * 1
    #28174705 - 02/06/23 10:29 AM (11 months, 14 days ago)

Quote:

Sulfurshelfsean said:
I've had lcs last years. One out out clean mycellium for six years but eventually they contam. I always use very little nute so I always wondered if they were in stasis. After about a year the culture liquid is completely clear. This looks like a much better way to store thank you for the write up. I'm going to try this.



ive noticed the same thing with low nute LCs. they colonize about 80% and then freeze


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Offlineside-eye
potate


Registered: 09/01/22
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Re: The OFFICIAL Stasis Storage Thread [Re: bakedbeings] * 4
    #28181951 - 02/11/23 08:50 AM (11 months, 9 days ago)

I dig it so I tried it.

I got some 5mL tubes with a rack, and wood stir sticks. Each one breaks into three or four pieces that fit in the tubes. Then I filled a bunch of the bottles with distilled water.



I soaked the stir sticks in the bottle for about 30 minutes, figured they needed a little more time than paper. Poured water out of the stir stick bottles, then PC'd everything in my IP for 45 minutes. Stir sticks onto a few plates. These pictures were taken two days apart, myc is already starting to get into the wood. Be sure all the broken pieces are ripped apart from each other, you can see how the Reishi plate has a big V shape that I didn't want to risk pulling apart.


Once this grows into the wood I'll drop em into the distilled water. I'll report back in 30 years to check if your tek actually works.


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Edited by side-eye (02/11/23 08:51 AM)


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Offlinenektar61S
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Re: The OFFICIAL Stasis Storage Thread [Re: side-eye] * 3
    #28182942 - 02/11/23 07:32 PM (11 months, 9 days ago)

Quote:

side-eye said:
I'll report back in 30 years to check if your tek actually works.




Excellent.

I have an experiment I've set up on here that should last 15 years. (vacuum packing very dry clone shrooms into 2 gram packs, then taking one every year and reporting back. First one will be pretty soon.


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InvisiblefahtsterM
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Re: The OFFICIAL Stasis Storage Thread [Re: fahtster] * 4
    #28182962 - 02/11/23 07:45 PM (11 months, 9 days ago)

Quote:

fahtster said:
That mak 118 is just about start knotting/pinning so weโ€™ll see how it does.  Youโ€™d think after being dried out for 10 months itโ€™d slow it down or wear out.  We shall see.  Ime, it does not






Bag did aight.



The question is if itโ€™s able to go 10 months dried out at room temp and come back and do that ^^^ whatโ€™s stopping it from going 10 more, or even more than that? 

I have dried out cultures in jars that are over a year old.  I can revisit this thread in a couple years and give those cultures a try at reviving.. see ya then :lol:


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Offlineside-eye
potate


Registered: 09/01/22
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Last seen: 1 month, 23 days
Re: The OFFICIAL Stasis Storage Thread [Re: fahtster] * 1
    #28190459 - 02/16/23 04:09 PM (11 months, 4 days ago)

1

Alright so I got two plates' sticks in water.


I ran into a few problems on this one. First was I let the plates colonize the sticks a little too much, especially on the Reishi plate. I tried picking it up with tweezers, but it was so stuck to the plate that I lifted up the whole puck of agar out of the dish. I tried cutting it out but it still grabbed any agar underneath the stick. Reishi was by far the worst, but all of the sticks lifted a bunch of myc off the plate. Here's how colonized the plates were:


Second, the wood sticks float. These tubes are 5mL so pretty short, and when I let go of the stick it floated about halfway out of the tube. I used the lid to push them down as I capped em. Longer tubes/sticks may not be as bad, and paper may not be as bad either. I'm worried they picked up some bac hitting the rim of the tube, so I will give this another go soon with paper vs sticks.

Lastly, my fault, I dropped some sticks in the wrong tube of water. Not sterile so I had to toss those. Don't work high, kids.


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Edited by side-eye (02/16/23 04:10 PM)


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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: The OFFICIAL Stasis Storage Thread [Re: side-eye] * 4
    #28190466 - 02/16/23 04:17 PM (11 months, 4 days ago)

What's the purpose of using wood or paper when a wedge of myc will suffice and last indefinitely? Seems like you're making it way more complicated than it needs to be.


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OfflineKabbal
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Registered: 06/22/22
Posts: 48
Loc: The Netherrealm
Last seen: 2 months, 30 days
Re: The OFFICIAL Stasis Storage Thread [Re: Guerrilla]
    #28199567 - 02/22/23 05:15 PM (10 months, 29 days ago)

Good shit here Boyo. I will post some results from 5 months ago when you told me about it.


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Offlinemycodependent
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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28221141 - 03/08/23 05:08 PM (10 months, 15 days ago)

oh fuck yeah I'm trying the fuck outta this shit ASAP.


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