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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: The OFFICIAL Stasis Storage Thread [Re: SeventhMushroom]
    #28612307 -

So at this point do we think LC stored in vials is fine? I’m thinking that it’d be easier/less likely to contaminate if using a vial or LC rather than trying to fish a piece of agar out of water.

Basically, which would be better: putting a small piece of agar in sterile water, or in LC solution?

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Re: The OFFICIAL Stasis Storage Thread [Re: 3.A.M]
    #28614679 -

3.A.M said:
And if anyone’s interested I’ve been using small autoclave pouches with great success for storing and drying grains, also use them for my swabs, they’re sanitary and literally designed to wick away moisture and dry out while at the same time maintaining a protective shield against contamination, I just pour grains straight from the jar in them when I do my g2g process, seal them and let them dry from inside the pouch, super cheap and almost too easy.


Did you find autoclave pouches that come sterile? Or are you sterilizing the empty pouches first, or just taking a chance since you'll be reviving it on agar anyway and can transfer away from any contaminant that survives dessication?

fahster has a custom Tyvek lid design which I am considering making without any silicone (I would just use them for drying, preferring inoculation without a needle). But if I could dry and store in a single cheap, lightweight, and small container, that would be pretty great.


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LAGM 2026 grow log | My library/trade list

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Re: The OFFICIAL Stasis Storage Thread [Re: Phaethon]
    #28614720 -

They’re absolutely not sterile and a couple times I’ve had to transfer away from contam but for ease and practicality they’re amazing, and no I don’t sterilise first but you could easily throw some in a jar and put them through a cycle if you like.
I originally got them as an alternative to wrapping home sterilised swabs in foil which isn’t sterile either so grains were just the next natural step, fahts methods are definitely tried, tested and guaranteed successful though if you want to avoid potential contam.


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: The OFFICIAL Stasis Storage Thread [Re: 3.A.M]
    #28615920 -

Cool, thank you! I think I will try that, probably starting by sterilizing the pouches (if that doesn't do something awful to the seal tape).


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LAGM 2026 grow log | My library/trade list

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Re: The OFFICIAL Stasis Storage Thread [Re: Phaethon]
    #28615940 -

Literally got some in the pc right now, glad we had this discussion!
I don’t use the seal tape anyway, did the first few times but it’s not really designed to come back off so I just masking tape it closed like most people do with swabs.
I’ll let you know if sterilisation warps the pouches at all but I don’t imagine it will, I’ve been using autoclaves for years in various industries, those pouches are pretty hardy.
You can also get roles of the stuff and heat seal them to any length you like if you want to go that avenue as well👍

Edit: pouches pc’d fine, they were a bit loose in the jar so they sagged down a little and will probably hold that shape but won’t effect function at all (:


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Clean MS from fresh fruits every time.
How I prep brown rice

Edited by 3.A.M (01/10/24 02:10 PM)

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Re: The OFFICIAL Stasis Storage Thread [Re: 3.A.M]
    #28618006 -

Nice, thanks for the update!

I've got a couple different pouch sizes in my Amazon cart, along with some bulk desiccant to speed up drying. Will post an update once I've tried it.

Thanks again for the idea! (It's funny - when I first got into the hobby, I was looking for something to enclose things in while pressure cooking, other than foil or rigid containers. I'd seen "autoclave bags" mentioned, and found those almost entirely for the purpose of sterilizing before disposal rather than use. Sterilization pouches and rolls are what I was looking for! I've used polypropylene tubing to enclose stacks of PP petri dishes, but they come out quite wet and stay that way. I'm going to try stacking them in 7.5" x 13" pouches.)


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LAGM 2026 grow log | My library/trade list

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Re: The OFFICIAL Stasis Storage Thread [Re: Phaethon]
    #28666652 -

:threadmonitor:


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LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

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Re: The OFFICIAL Stasis Storage Thread [Re: fossilshark]
    #28670112 -

:tripnoob:

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Re: The OFFICIAL Stasis Storage Thread [Re: Copasetic Panda] * 1
    #28885618 -

Where did we land on the "is wood necessary or will an agar wedge suffice" argument?

I have been meaning to do something with my small collection of slants and some spore samples I may not get to for a while.


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LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

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Re: The OFFICIAL Stasis Storage Thread [Re: the_chosen_one] * 2
    #29035761 -

the_chosen_one said:
How is it a person can be here for years and all the sudden something like this pops up and it was started a year ago? :confused:

I've become a huge fan of the 2ml cryovials and the Castellani method.


OR

Just forget some syringes in the fridge for 13 years. :lol: We'll see these in a decade or so.


Works for spores though. :shrug: I've labelled these Tamp 13 for just that reason lol.


Thanks to Tree Frog for the link!
:manofapproval:





That moment when i realized it was time to replace my whole slant collection, those 2mL vials came in handy. Everything left at room temp, cool and dark in my situation. Fuck yeah! :heart::heart:


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:ancientaliens:

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Re: The OFFICIAL Stasis Storage Thread [Re: mycosispeon] * 1
    #29035762 -

mycosispeon said:
Where did we land on the "is wood necessary or will an agar wedge suffice" argument?

I have been meaning to do something with my small collection of slants and some spore samples I may not get to for a while.



Either is fine, i prefer agar.. Different strokes for different folks:heart::heart:


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:ancientaliens:

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Re: The OFFICIAL Stasis Storage Thread [Re: astra] * 6
    #29041055 -

Just can’t beat dry grain spawn. No need to over complicate the drying process…throw some in a petri dish or some other sterile container for a couple days and then store as desired.  Just waking these up after a year.


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🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: The OFFICIAL Stasis Storage Thread [Re: Mycolorado] * 2
    #29043691 -

Mycolorado said:
Just can’t beat dry grain spawn. No need to over complicate the drying process…throw some in a petri dish or some other sterile container for a couple days and then store as desired.  Just waking these up after a year.



I did the exact same thing the other day.
Seemed so much easier than playing around with distilled water and cryo vials.
I mean, I’ve got a bunch of plates stored either way, so a couple more here and there kinda seems negligible.



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= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

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Re: The OFFICIAL Stasis Storage Thread [Re: MycCycle]
    #29043894 -

I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: The OFFICIAL Stasis Storage Thread [Re: MycCycle]
    #29043906 -

Possibly dumb question - can I really store colonized, dried grains in a petri dish? Do they get stored in the freezer?  If so, this is going to get tested soon.


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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: The OFFICIAL Stasis Storage Thread [Re: ReverendMyc] * 4
    #29043914 -

ReverendMyc said:
I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.


They need to be cut off from drying at some point.. when I have them just sitting in a jar with large open filters, they tend to die ~12-18 months.  When I put them in a sealed container, they have lasted 2+ years.. I haven’t run into the limit yet.  Good rule of thumb is that if the starch inside the grain has dried and turned to dust/emptied, the myc is dead

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Re: The OFFICIAL Stasis Storage Thread [Re: fahtster]
    #29043918 -

fahtster said:
ReverendMyc said:
I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.


They need to be cut off from drying at some point.. when I have them just sitting in a jar with large open filters, they tend to die ~12-18 months.  When I put them in a sealed container, they have lasted 2+ years.. I haven’t run into the limit yet.  Good rule of thumb is that if the starch inside the grain has dried and turned to dust/emptied, the myc is dead


:takingnotes:


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: The OFFICIAL Stasis Storage Thread [Re: fahtster] * 2
    #29043976 -

fahtster said:
ReverendMyc said:
I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.


They need to be cut off from drying at some point.. when I have them just sitting in a jar with large open filters, they tend to die ~12-18 months.  When I put them in a sealed container, they have lasted 2+ years.. I haven’t run into the limit yet.  Good rule of thumb is that if the starch inside the grain has dried and turned to dust/emptied, the myc is dead


Good info. Thanks fahtster.
I put some PE and semps grains into cryo tubes in April 2022 after drying in the jar for a month or so. I will put them to agar next time I start up the ffu. Looking up those dates I discovered that the PE was the culture that produced my largest blob ever in a Faht-loc bag. I would sure love to do that again.

It would be really useful for dehydration and storage methods vs potency testing.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: The OFFICIAL Stasis Storage Thread [Re: Mycolorado]
    #29044017 -

Mycolorado said:
Just can’t beat dry grain spawn. No need to over complicate the drying process…throw some in a petri dish or some other sterile container for a couple days and then store as desired.  Just waking these up after a year.



:nowaydude::mindblown::heart::heart:

Edited by astra (12/03/24 01:00 PM)

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Re: The OFFICIAL Stasis Storage Thread [Re: ReverendMyc]
    #29044072 -

ReverendMyc said:
I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.


I'm no expert and I've certainly not put 2yo, thoroughly dried grain under a microscope, however:
Some materials, i.e. our substrates, basically turn hydrophobic after having reached a certain level of dryness. They need a certain amount of moisture to absorb more moisture.
How about soaking the grain in a cryo vial with sterile water for 24 hours, then put it on agar?


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

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Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   MagicBag.co Certified Organic All-In-One Grow Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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