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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27942564 -

What's you favorite color?!

To use in your agar...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27942569 -

Blue! Though I’d prefer the myc not eat it.

Here’s a T2 I’m really liking the look of. I think after another transfer it should be good.



Then I have a question: this is a T4 and it keeps growing out the same way every time I transfer it. Aggressive growth in one direction and slow growth in the other. I always take the most organized sample I can from the aggressive side, but lo and behold it grows out the same way. Should I keep transferring? It’s definitely a little tiny bit less chaotic each time, but a fully lopsided T4 seems weird to my inexperienced hand.



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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Edited by Smellyhobbit (09/09/22 10:07 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PBJ710]
    #27942683 -

You should use a pc to sterilize your glass Petris the pressure and steam is what sterilizes and kills any heat resistant endo spores if I’m not mistaken.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #27942730 -

Smellyhobbit said:
Blue! Though I’d prefer the myc not eat it.

Here’s a T2 I’m really liking the look of. I think after another transfer it should be good.



Then I have a question: this is a T4 and it keeps growing out the same way every time I transfer it. Aggressive growth in one direction and slow growth in the other. I always take the most organized sample I can from the aggressive side, but lo and behold it grows out the same way. Should I keep transferring? It’s definitely a little tiny bit less chaotic each time, but a fully lopsided T4 seems weird to my inexperienced hand.




What the heck is Vader, I'm agaring Jedi so...?

:tongue2:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27942739 -

Fingers crossed I start seeing plate pins this week from a couple germ plates. I believe I see knots forming on a few. Here's a B+ I took four transfers from, it didn't contam and kept growing clean:



B+ germ plate, no transfers taken yet:



Two in the front are RustyWhyte germ plates looks like knots starting to form too:



So looks like my first pins will be plins! I'll take biopsies and then possibly put the poked plins on agar too just to see the growth difference. I'm a believer in using a needle biopsy where I can. It has worked great for me.


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****FUGGIN' FROGZ****

Trade List

Edited by Cob (09/10/22 12:12 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bab.D]
    #27942823 -

So, after a whole night of research, I found out that my PC only cooks at 10PSI, and I was sterilizing with 15PSI times, which is probably the cause of the bacterial contamination.

Any suggestions on the time I would need to sterilize dishes under 10psi? I've only found grain sterilizing time (4 hours), but nothing on petri/agar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bab.D]
    #27942953 -

Bab.D said:
So, after a whole night of research, I found out that my PC only cooks at 10PSI, and I was sterilizing with 15PSI times, which is probably the cause of the bacterial contamination.

Any suggestions on the time I would need to sterilize dishes under 10psi? I've only found grain sterilizing time (4 hours), but nothing on petri/agar.


I guess I'd probably double it? So 40mins instead of 20mins.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bab.D]
    #27943352 -

Bab.D said:
I use no-pour, and PC 10 petris inside a closed plastic bag (to avoid wasting foil).


Wondering if having them in a sealed plastic bag is preventing the PC from fully venting around your dishes leaving you with an incomplete sterilization. If you have any mushroom growing bags try using those but leave it unsealed. It will seal itself on cooldown. If not you might try one round with foil just to see if that’s the issue.

Others might do this successfully though so I’m just throwing out an idea. I wrap my pre-pours in foil but I reuse that foil until it’s torn and unusable.

Edited by Southerner (09/10/22 10:39 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PBJ710]
    #27943363 -

PBJ710 said:
I'm having a similar issue with bacteria in my poured glass petris, but not in my disposable plastic petris. 

As I'm still new to glass plates, I'm convinced that I'm not getting the plates fully cleaned/sterilized despite wrapping in foil and using 1h @ 350f in a toaster oven.  Maybe I'm overwrapping them or doing something that's preventing them from getting all the way up to sterilization temps. :shrug:

I left the poured glass plates out in front of my FFU for an hour to set/cool before bagging them upside down.  They were poured at 118f and the plates/lids were still warm (~100f) from being processed in the oven.  I was hoping having the petris be near the same temp would stop the condensation, but it didn't help much at all.


I PC my glass petris before pouring. I don't trust anything else.


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Repaying my karmic debt to the Shroomery.
Looking for a Pan cambo print.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Southerner]
    #27943377 -

Yeah I tried reusing foil, but it always has some micro torns that can only be seen if you place some light behind, so I stopped with that.

I actually don't believe that the bag prevents much, since agar inside them is supposedly boiling again from the temperature, and they still get as much condensation as when I used the foil.

Ps. I got similar contamination when I used the foil too. Now that I remember I had to discard a whole petri batch once a couple of months ago!

Quote:
I guess I'd probably double it? So 40mins instead of 20mins.


It's probably more. According to the pressure chart someone published here for grains for example, a jar that took 90min to sterilize under 15psi, would take 240min to do so under 10psi.... Thats like a 2.7X increase.

So for the 20min, theoretically it would be somewhere around 60min. But I'm not sure if the agar would resist so much boiling without evaporating the water from it?

Awestruck said:
I PC my glass petris before pouring. I don't trust anything else.


I would gladly do that If I had the money to buy a flowhood lol

Edited by Bab.D (09/10/22 10:55 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #27943420 -

:lol:

Nichrome had an adjacent tub spore up another. They “invaded.”


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #27943438 -

shroomsurf said:
What the heck is Vader, I'm agaring Jedi so...?

:tongue2:


Quote:
LulzSwag said:
Vader
Cross of Melmak & Blue Meanie




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Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27943604 -



Decided to start playing with selection and isolation, this is my favorite plate so far.

The culture was cloned and further selected from a B+ MSS grow.


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༔༑Entwined༔with༔all༔nature's༔creations༑༔

Edited by abs0lem (09/10/22 02:15 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: abs0lem] * 1
    #27943810 -

I've been picking plins for a few days now. I also have 3 grain jars waiting for inoculation.

Can I just cut some wedges off this plate and stick in the jars?


Or should I wait and use new growth from the clones?


Not sure i did this right, this clone keeps growing and is now touching the plate cover


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bismillah] * 1
    #27943890 -

those photos are awesome - macro lens ftw :super:
you could take wedges from that plate if youd like
if it is a germ plate i personally wouldnt, but if its t1 or t2+ im sure it would be fine
overgrown but looks clean to me


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helpful links
my FH build

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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident]
    #27943931 -

Thanks! It's T1. My eyesight has gone to shit in the past couple of years, macro lens helps me see :smile:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27944180 -

Reishi, getting slanted and sent to grain tomorrow



--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27944198 -

Rotnpins said:
Reishi, getting slanted and sent to grain tomorrow




Very nice, got a good symmetrical culture there.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27944214 -

Rotnpins said:
Reishi, getting slanted and sent to grain tomorrow




Nice!


--------------------
Repaying my karmic debt to the Shroomery.
Looking for a Pan cambo print.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 4
    #27944274 -

Who's gonna be doing a lot more agar work soon?



ME! :dancer:


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Shop: Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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