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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27822621 -

Very awesome information on plins nektar61 and nichrome. I've got a Tidal Wave plate that is knotting up nicely, so hopefully I'll have my first plins soon. I found your guys posts very helpful.

I was using a torch but recently got an alcohol burner. I've noticed that both leave a black "soot" on the blade.. I was wiping it off with an alcohol swab at first,  but I have a feeling this is counter productive since flame sterilizing is supposed to be better than isopropyl.. is the soot something I need to worry about? Or do I need to change my technique somehow?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27822622 -

Plate pins are cool.



--------------------
Question everything, it's very responsive.

culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27822625 -

Rotnpins said:
Very awesome information on plins nektar61 and nichrome. I've got a Tidal Wave plate that is knotting up nicely, so hopefully I'll have my first plins soon. I found your guys posts very helpful.

I was using a torch but recently got an alcohol burner. I've noticed that both leave a black "soot" on the blade.. I was wiping it off with an alcohol swab at first,  but I have a feeling this is counter productive since flame sterilizing is supposed to be better than isopropyl.. is the soot something I need to worry about? Or do I need to change my technique somehow?




Wipe the blade off right after transfers and flame, then right to the new transfer, then wipe then flame then transfer, then wipe then flame then transfer, over and over and over till the cows come home. At least soot is sterile. Wiping is just potentially contaminating a flame sterilized implement. Dirty is fine so long as dirty is sterile. Clean is not the same as sterile.

Flame>Agar>wipe>flame>agar>wipe


--------------------
Question everything, it's very responsive.

culture storage

Edited by Nichrome (06/16/22 02:08 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 2
    #27822644 -

Rotnpins said:
Very awesome information on plins nektar61 and nichrome. I've got a Tidal Wave plate that is knotting up nicely, so hopefully I'll have my first plins soon. I found your guys posts very helpful.

I was using a torch but recently got an alcohol burner. I've noticed that both leave a black "soot" on the blade.. I was wiping it off with an alcohol swab at first,  but I have a feeling this is counter productive since flame sterilizing is supposed to be better than isopropyl.. is the soot something I need to worry about? Or do I need to change my technique somehow?



Never wipe a blade after flaming! The soot is sterile

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27822660 -

Awesome! Thanks guys. Makes a lot of sense. And now I know not to let the soot bother me :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27822668 -

Now that I’ve cling wrapped my agar plates twice around it seems like I’m having more issue…slower growth and some condensation that’s staying/leaving a drop of water on agar. I’m not saying I’m blaming y’all for what’s the correct way to do things I’m just saying I’m blaming y’all.(jk)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 2
    #27822699 -

No pics today, just wanted to vent that I had a frustrating agar session today where out of 20ish transfers, 7 had agar wedges get stuck too high above about the blade and I couldnt transfer it to the receiving plate.

Shit really grinds my gears :smokerage:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 2
    #27822750 -

Nichrome said:
Opening them up brings in fresh air, rich with brand new oxygen. A fresh supply of oxygen allows for faster metabolism.

Sometimes opening up the plate and exchanging the air can trigger pinning too. A neat trick if you hunt plate pins or want to see traits in an unknown culture like cap color or shape.



I recently had two jars go bacterial, I brought them outside and made a small bed with one and forgot about the other. I had taken the top off but turned it upside down so bugs couldn't get in. Holy crap the mycelium sped up so much its almost colonized already. Faster than my ones inside.  I guess it was all the fresh air and warmer temp.


--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc]
    #27822776 -

Mealworm flour agar.



Trying to see if I can get this Beauveria culture to run a little faster. I believe the little diamonds to be enzymes.


:gump:


--------------------
Question everything, it's very responsive.

culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27822857 -

Rotnpins said:
Very awesome information on plins nektar61 and nichrome. I've got a Tidal Wave plate that is knotting up nicely, so hopefully I'll have my first plins soon. I found your guys posts very helpful.

I was using a torch but recently got an alcohol burner. I've noticed that both leave a black "soot" on the blade.. I was wiping it off with an alcohol swab at first,  but I have a feeling this is counter productive since flame sterilizing is supposed to be better than isopropyl.. is the soot something I need to worry about? Or do I need to change my technique somehow?



A lot of us use a butane brazing torch:
https://www.amazon.com/Blazer-Stingray-Butane-Torch/dp/B07C1S6KVZ

Hotter than an alcohol lamp and doens't soot as much. Makes blade red hot way quicker than alcohol lamp.

Make sure you get butane refills that have a compatible stem for your unit. Look for "people who bought this also bought..." or find out if it's short or long stem.

When I moved from alcohol torch to this, things got better quick.

You don't have to use this one, but don't use the cheapest one. Don't cheap out on a compressed gas tank you'll hold in your hand to flame metal with. Read reviews, get one with 4 and a half stars, and read the 1 star reviews to see if any blew up.

When you say "torch" do you mean one of these? If so, why did you go from a torch to an alcohol lamp? Seems like a step back.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27822882 -

Yes. That was the type of torch I was using... idk, I liked the idea of the flame staying lit the whole time. Yes, I do realize that I could keep the torch lit the whole time, however of the 5 torches I have at home they all had one issue or another (not staying lit, not lighting properly and needing to jump the flame with a lighter, etc).. I may end up getting another torch exclusively for mycology purposes at some point. Just need to make sure the pot-heads in my household don't get their hands on it,  otherwise it will end up with the same issues as the other ones :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27823018 -

Rotnpins said:
Yes. That was the type of torch I was using... idk, I liked the idea of the flame staying lit the whole time.



I don't keep it lit all the time. Seems dangerous. If you knock it over, could quickly start a fire.

Or if it running continuously stresses the cheap design and makes it explode.

I just use it when I need to flame something. It's not hard to use with one hand.

Edited by nektar61 (06/16/22 05:46 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27823023 -

I just figured my question (agar related) might get more answers if I shared it here: https://www.shroomery.org/forums/showflat.php/Number/27823020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: asteephill]
    #27823062 -

Thanks nektar its too bad i threw that plate away cus there was a fatty that got big over 2 days. Feels fast for ape

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27823128 -

nektar61 said:
Rotnpins said:
Yes. That was the type of torch I was using... idk, I liked the idea of the flame staying lit the whole time.



I don't keep it lit all the time. Seems dangerous. If you knock it over, could quickly start a fire.

Or if it running continuously stresses the cheap design and makes it explode.

I just use it when I need to flame something. It's not hard to use with one hand.



Yeah.. idk.. that's really the main reason I switched to an alcohol burner :shrug: something about continually touching something outside my SAB makes me cringe... at least with the burner I can just move one hand out of my SAB and then right back in without touching anything.

I know the goal is to never have your hand move or hover above your plate,  but even so, I still try and keep my hands as sanitary as possible the whole time.... even if I wipe the torch handle down first, I still feel like it's going to screw everything up..

Honestly, my sterile technique is probably one of the things I'm "best at".. I don't mean that as a boast,.. it still has room for improvement, but I'm already really OCD and every tiny thing I do that could be a contam vector gives me the "cringe" feeling :lol:


Edit: thinking about using one of these... I saw a SAB video where the person used one of these and left it lit


Edited by Rotnpins (06/16/22 07:49 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27823132 -

Can I post my agar pics here for help???

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MOUSIE]
    #27823137 -

Yup, you sure can

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27823142 -

Rotnpins said:
Yup, you sure can



Goodlookin out…

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MOUSIE]
    #27823152 -

This was MSS to raz media premix MEA on 6/9 …first 3 are PE6, last 3 are JohnAllen. Kept in tub with old lady ass heating pad, temp always says like 78f… no pc tek agar, steamed my shït for like 1hr 45mins (On God), poured in front of diy flow hood in my kitchen🤣🤣🤣. Got syringes from a vendor on SHROOMERY, shout out MUSHROOMMANMYCOLOGY… SO, what my shït look like y’all… I’m kool or naw’???

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MOUSIE]
    #27823160 -

Where my pics @…

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