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Dendrocopos

Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 2 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]

1
#27767131 - 05/07/22 09:52 AM (4 years, 4 months ago)

I'd still buy her dinner

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MysticMycologist
Dirt Sherpa

Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]

1
#27767132 - 05/07/22 09:53 AM (4 years, 4 months ago)

For sure. Not being ageist. I’m quite seasoned myself.

--------------------
Two eyes to look, One eye to see.
Prying open my third eye

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Culliganman360
Stranger

Registered: 06/05/19
Posts: 854

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]
#27767133 - 05/07/22 09:54 AM (4 years, 4 months ago)

A chicken dinner :rofl:

:rofl:

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Rotnpins
🤮 Rotten-Pins 🍄

Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 3 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Culliganman360]
#27767135 - 05/07/22 09:55 AM (4 years, 4 months ago)

Culliganman360 said:

A chicken dinner

:laugh2:

:friedchicken:

:spazzychicken:

--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras:

bigfootscreepyuncl
Stranger

Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MysticMycologist]

1
#27767139 - 05/07/22 09:57 AM (4 years, 4 months ago)

Quote:

MysticMycologist said: Now she is a seasoned chicken.

:rofl2:

--------------------

I

:heart:

5318008

NOT a virgin!

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Dendrocopos

Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 2 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27767167 - 05/07/22 10:15 AM (4 years, 4 months ago)

Someone wants to get will smith'd

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LotKid
Never.Trust.A.Prankster

Registered: 01/07/17
Posts: 8,883
Loc: Shakedown St.

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]
#27767179 - 05/07/22 10:25 AM (4 years, 4 months ago)

:lol:

--------------------

:mushroom2:

:mushroom2:

LotKid's PE Tips :shpongle:

:shpongle:

LotKid's Chocolates :mushroom2:

:mushroom2:

:dancingbear:

:dancingbear:

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,604
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27767183 - 05/07/22 10:29 AM (4 years, 4 months ago)

OK, I'd love to talk about actresses and avatars all day, but I have an agar question.
One on the left: contam or not. If so, why?
One on the right: just sharing for fun. Dried to fruit leather. I'm going to put it in the fridge for a year and then try to reanimate it. (IT'S ALIVE!) Who knows. Maybe that works better than slants. haha.

--------------------
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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BleuMojeaux
Futzer of Things

Registered: 03/20/22
Posts: 71
Last seen: 4 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]

1
#27767467 - 05/07/22 02:44 PM (4 years, 4 months ago)

First time playing with agar :grin:

:grin:

:grin:

:grin:

From what I've read, clear mycelium is a nutrient issue in your recipe. Any suggestions as to what I should do with these?

Different plate, same recipe MEA 10/10/500

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Infinite Monkeys

Registered: 09/01/21
Posts: 408

Re: AGAR ENVY! (Anything and All things agar!)

1
#27767626 - 05/07/22 05:19 PM (4 years, 4 months ago)

Rotnpins said:

Infinite monkeys 🐒 I love your avatar did you make it?

Shroomagician made it.

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Infinite Monkeys

Registered: 09/01/21
Posts: 408

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]
#27767655 - 05/07/22 05:39 PM (4 years, 4 months ago)

Dendrocopos said:

Haha dude. This is exactly what was happening to that culture after transferring. Did you move the culture from a drawer to a table or something ? Cause I've had it happen more when i opened the plate/let it sit on the table/ put it in the fridge etc.

It was on a bookshelf the entire time. Temperatures tend to fluctuate wildly here if that could contribute to this type of growth, idk.

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SirPsycho
Purple Belt in Google-Fu

Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
Last seen: 3 months, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Infinite Monkeys]

1
#27767662 - 05/07/22 05:49 PM (4 years, 4 months ago)

There is a House in New Orleans
They call the rhizing sun

Clone from my book grow, the bunny mushroom that's my avatar

--------------------
:pm:

:pm:

Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens :pm:

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Balance in life is like running on ice.

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"Mist your balls and fan your asshole" - Pandaskis, 2023

Edited by SirPsycho (05/07/22 05:49 PM)

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,604
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Infinite Monkeys]
#27768105 - 05/08/22 02:32 AM (4 years, 4 months ago)

Infinite Monkeys said:

Rotnpins said:

Infinite monkeys 🐒 I love your avatar did you make it?

Shroomagician made it.

I like their stuff. Never seen it.

--------------------
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras:

Infinite Monkeys

Registered: 09/01/21
Posts: 408

Re: AGAR ENVY! (Anything and All things agar!)

1
#27768953 - 05/08/22 05:16 PM (4 years, 4 months ago)

nektar61 said:

Infinite Monkeys said:

Rotnpins said:

Infinite monkeys 🐒 I love your avatar did you make it?

Shroomagician made it.

I like their stuff. Never seen it.

Isn't it neat?

Pixel art is a lovely and underappreciated kind of art.

I've finally poured plates that are clear and sediment-free.

The clarity was somewhat reduced by the food coloring so the following batch will be be uncolored.

Edited by Infinite Monkeys (05/16/22 01:44 AM)

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Luminous7

Registered: 01/26/16
Posts: 1,456
Loc: Canada
Last seen: 2 days, 14 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BleuMojeaux]
#27769077 - 05/08/22 07:33 PM (4 years, 4 months ago)

BleuMojeaux said:

First time playing with agar From what I've read, clear mycelium is a nutrient issue in your recipe. Any suggestions as to what I should do with these?

Different plate, same recipe MEA 10/10/500

Ive also been wondering about the clear thin myc.

Ever since i moved living spaces I rarely see ropey rhizo. Only that really thing clear myc.

--------------------

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Rotnpins
🤮 Rotten-Pins 🍄

Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 3 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Luminous7]
#27769121 - 05/08/22 08:22 PM (4 years, 4 months ago)

Luminous7 said:

Ive also been wondering about the clear thin myc.

Ever since i moved living spaces I rarely see ropey rhizo. Only that really thing clear myc.

I was getting plates that looked like that, and they filled up in just a few days.. it was a nutrient issue... the mycelium is stretching out quickly looking for food..

***
Does this plate look alright? I'm planning on trying to get a plate pin to transfer.. is there a particular reason why the center looks this way? I was thinking it might be due to temperature change (cold->warm) but I want to be sure

--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Edited by Rotnpins (05/08/22 08:25 PM)

Extras:

bigfootscreepyuncl
Stranger

Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge

Re: AGAR ENVY! (Anything and All things agar!)

1
#27769126 - 05/08/22 08:30 PM (4 years, 4 months ago)

No. That plate is heavily enmeshed with something bad. You might still be able to get a plate pin, or maybe clean it up, but I wouldn't send that to grain

--------------------

I

:heart:

5318008

NOT a virgin!

Extras:

Rotnpins
🤮 Rotten-Pins 🍄

Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 3 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: bigfootscreepyuncl]
#27769129 - 05/08/22 08:33 PM (4 years, 4 months ago)

K, thanks.. I had a feeling something was off, but I was holding out hope because I inoculated a few jars with the T2 and they turned out alright..

Eh, I've got some better clones going now anyway, so I'll probably just toss it.

--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras:

nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,604
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rotnpins]
#27769289 - 05/08/22 11:51 PM (4 years, 4 months ago)

Rotnpins said:

Does this plate look alright?

Reminds me of pennacilin mold. Might even be that, before sporilating (turning green). I'd not open it, tape it up more, and throw it out. I'll open a bacterial plate, but not something with mold that might be dropping spores. Some people here will do that, but I'm worried about contamming the whole room.

--------------------
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras:

MyKool
Stranger

Registered: 05/04/22
Posts: 3
Last seen: 4 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nektar61]
#27769454 - 05/09/22 05:56 AM (4 years, 4 months ago)

I’m new to all this and have tried my hand a LC’s. A simple LME culture worked well for me in the beginning, then I tried all the super power LC’s out there. Couldn’t get a clear culture for the life of me, so have switched to agar.
Unlike the liquid culture, it is going better than expected. I really like the Idea of storing a LC in the fridge for a year so decided to try again emboldened by the advice to take a sample from my plates to inoculate my culture to eliminate the issues of using MSS.
Here is the problem. After going back to what works and making 6 different cultures successfully with spores, I then tried to transfer small clean samples from agar to Jars of the same batch of LC. They became slightly cloudy after only a couple hours while the MSS jars are still clear and healthy.
I am using 1.4g LME and .75g peptone filtered twice. So my question finally…. Does agar make your water cloudy?
I have heard along the way that sometimes it will clear up as the mycelium grows but have not experienced that previously. Any viable feedback would be greatly appreciated. You guys have already helped more than you so let me end this by saying Thanks!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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