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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27758505 -

Anyone ever successfully take transfers from a plate with trich satellites?

I don't wanna lose the cultures


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โ€œThe strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.โ€

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Re: AGAR ENVY! (Anything and All things agar!) [Re: anatomality] * 1
    #27758507 -

Yes


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27758597 -

LotKid said:




:holyfuckdude:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: anatomality] * 1
    #27758598 -

anatomality said:
Anyone ever successfully take transfers from a plate with trich satellites?

I don't wanna lose the cultures



I just cut those bitches out. Unconventional, but works if you're a cheapskate.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos] * 1
    #27758769 -

Small wedge of agar suspended in sterile distilled water appears to be working fine though these are less than a year old. Real test will be when I wake it up. A very fine halo of myc extends off the disk then stops.



Agar work still has to be one my favorite aspects of the hobby. Never grow tired of it.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27758806 -

D3_Myc said:
Small wedge of agar suspended in sterile distilled water appears to be working fine though these are less than a year old. Real test will be when I wake it up. A very fine halo of myc extends off the disk then stops.



Agar work still has to be one my favorite aspects of the hobby. Never grow tired of it.





Nice mate, had a thought the other day. I have some pretty similar vials and have had some cultures stored for a few weeks. I wrapped the tops with clingfilm just because and threw them in a ziplock and put them in a tote.

Looked at them the other day and realised itโ€™s probably best to store these upright at all times so the agar wedge stays in the water.

The agar wedge could go AWOL if say, stored on their side.

I guess what Iโ€™m trying to say is do you think itโ€™s detrimental for the wedge to not be completely submerged in the sterile water at any point?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SwabMarley]
    #27758868 -

Should I make more transfers from this?



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wildcat1883]
    #27758876 -

What is it? I'd take one transfer and drop that whole thing to grains. Best of both worlds :awesomenod:


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SwabMarley]
    #27758877 -


I am not sure whether to throw this out or not. It's supposed to be cubensis mycelium. I took two transfers from a MS plate (which I threw). It seemed fine in the beginning but the way it's growing looks weird to me, almost looks like trich. The first two pictures are the second transfer plate, and this is what the first transfer after germination plate is looking like:

Should I throw it out? Keep in mind these are glass plates sealed with cling wrap, they are pretty much airtight, so mycelium isn't getting any gas exchange.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 5
    #27758891 -



Ropes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mimir] * 1
    #27758913 -

mimir said:

I am not sure whether to throw this out or not. It's supposed to be cubensis mycelium. I took two transfers from a MS plate (which I threw). It seemed fine in the beginning but the way it's growing looks weird to me, almost looks like trich. The first two pictures are the second transfer plate, and this is what the first transfer after germination plate is looking like:

Should I throw it out? Keep in mind these are glass plates sealed with cling wrap, they are pretty much airtight, so mycelium isn't getting any gas exchange.




Begin anew.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27758915 -

A lot of time, unless it's a culture you know performs amazing but somehow got fucked, it's overall the right thing to toss that shit. I had a culture that was garbage looking. Spent about 50 plates and don't know how much time trying to save it. If I had just started from scratch, would've saved a lot of resources. With weird viral plates cultures often perform weird even when saved, but maybe that's just me.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos]
    #27759200 -

Thatโ€™s what I was thinking about doing bigfoot. So it shall be done. And itโ€™s HU.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wildcat1883]
    #27759221 -

Would you throw these in grain?



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wildcat1883] * 2
    #27759225 -

nope. Too spiky and zero uniformity. If you can, try and grab one of the aerial threads/ropes of mycelium and just transfer that


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos] * 1
    #27759287 -

Dendrocopos said:


Ropes



Ooh you got a good one goinโ€™ there. :awesomenod:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wildcat1883] * 1
    #27759293 -

Wildcat1883 said:
Would you throw these in grain?





Definitely not. Mycelium running/reaching away like that is a big red flag. You want nice, even, relatively symmetric growth from the initial transfer. Do what bigfoot said.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Boozie] * 2
    #27759305 -



Got a little cockeyed on the right stack. Gotta pay more attention when leveling my rack!


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 1
    #27759468 -

LotKid said:
Pretty sure morelman was doing a freezing test.

And freezing is talked about in one of the mush cult books i have.





https://www.shroomery.org/forums/showflat.php?Cat=0&Number=27759467&page=0&vc=1#27759467

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 1
    #27759788 -

SirPsycho said:
Found the thread I was reading recently.

https://www.shroomery.org/forums/showflat.php/Number/27738850#27738850

That comment sums it up and links further on



I think this is the tek that these threads refer to.
Distilled water; A tribal way for long term storage

I have it on my list of things to try, but haven't yet.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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