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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,580
Loc: Hollywoo

Last seen: 18 hours, 36 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: TheConfluence]
#27675003 - 02/27/22 01:30 AM (4 years, 5 months ago)

TheConfluence said:

My albino took!

Surface of Titan, moon of Saturn:

--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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aosod
Stranger

Registered: 12/02/21
Posts: 14
Last seen: 3 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nektar61]
#27675067 - 02/27/22 04:53 AM (4 years, 5 months ago)

Hey guys! I´ve been trying to get into agar and made some test plates and couple transfers, however i´ve been getting weird yellow spots on the inside of the plates(not on the first ones tho). I wipe my glass petris with bleach/water solution, then pc for 20 min and dry in the oven.
Ill put couple pictures i dont know if you can see the problem in those.

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dyel

Registered: 10/15/21
Posts: 922
Last seen: 2 months, 9 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aosod]
#27675121 - 02/27/22 06:50 AM (4 years, 5 months ago)

are these cubes or something else? I was concerned about some of my older enoki cultures getting a yellow brownish hew but after looking it up it turned out it's ok

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sandman420
Saint PP

Registered: 06/17/04
Posts: 5,393

Re: AGAR ENVY! (Anything and All things agar!)

[Re: dyel]
#27675222 - 02/27/22 08:59 AM (4 years, 5 months ago)

Why the green agar eww. It's so hard to see what's happening. Anything but green homey.

That is probably some type of mold or intermeshed mold. Cubensis mycelium is not yellow under any circumstances (barring green agar)

--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Will Power
Gardener

Registered: 08/05/18
Posts: 31
Loc: South of heaven
Last seen: 2 years, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27675288 - 02/27/22 10:13 AM (4 years, 5 months ago)

Gymnopilus luteofolius knotting on the plate already. Gonna put it to grains tomorrow. Finally got dome decent OG melmak myc going too.

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aosod
Stranger

Registered: 12/02/21
Posts: 14
Last seen: 3 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sandman420]
#27675290 - 02/27/22 10:14 AM (4 years, 5 months ago)

sandman420 said:

Why the green agar eww. It's so hard to see what's happening. Anything but green homey.

That is probably some type of mold or intermeshed mold. Cubensis mycelium is not yellow under any circumstances (barring green agar)

I guess green food coloring was the only one my nearby store had, maybe i will find a different colour. For me it seems that the yellow thing is on the petri dish maybe its hard to see on pictures.I have some plates that i poured but did not put any spores into and they have the same problem, is it something with my cleaning or PC process? Or is it possible that condensation from them being on PC leaves some residue on the plates?

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,950
Loc: South Africa
Last seen: 1 day, 7 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aosod]
#27675291 - 02/27/22 10:16 AM (4 years, 5 months ago)

I'm guessing it's bacteria but without getting better pictures it's difficult to say.

Bactiera is generally related to your technique when working with the open plates.

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TheConfluence
Stranger

Registered: 12/13/21
Posts: 157

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sandman420]
#27675300 - 02/27/22 10:23 AM (4 years, 5 months ago)

I think it likely is contaminated...
I used a purple food coloring for my MEA and as this mycelium developed it turned a yellowish orange again much like the original undyed MEA would look.

I don't really know though because I'm still very new to this, but the other plates I have had that exhibited this behavior all wound up super bacterial or straight up got the Trich.

So yeah probably either today or tomorrow I'm gonna try spreading this little guy out to 8 more plates (this is also coincidentally my most aggressive genetic in terms of it's ability to myceliate grain)

--------------------
When two people dream the same dream, it ceases to be an illusion.

Philip K. Dick
Lies, Inc. (1984)

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Dendrocopos

Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 2 years, 2 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#27675302 - 02/27/22 10:25 AM (4 years, 5 months ago)

Could i get some critique on my plates ? I have been getting craazy rosecomb/mutants that cover the entire tub. I am starting to get extremely paranoid about anything that doesn't look gorgeous.

Looks good, but rhyzomorphs weirdly lift into the air like they are escaping from something.

weirdly ununiform growht

3D/fuzzy look. Looks like bacterial bubbling.

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sandman420
Saint PP

Registered: 06/17/04
Posts: 5,393

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]

1
#27675311 - 02/27/22 10:33 AM (4 years, 5 months ago)

It's possibly some kind of mycoparasite or mycovirus.

It's the least researched and knowledgeable area of home psychedelic cultivation because all of the scholarly work is on edible commercial mushrooms so far and it takes serious equipment to research (genetics, dna id, etc)

But you can see the mycelium is making multiple layers on top of each other in the first pic.

Getting mutations and blobs and stuff across multiple strains would be very statistically unlikely unless you were spraying some chemicals or something. That plus the strange mycelium leads me to believe it could be in the realm of the mycoparasite/virus.

But that's just more questions not really an answer right my bad...

I probably sound like a broken record and something like this should be super rare....

but there are more people growing mushrooms at home then ever before and the way we share our spores and make prints is ripe for mycovirus and mycoparasite transmission so it makes sense for it to grow exponentially and be more common over time.

--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (02/27/22 10:36 AM)

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Dendrocopos

Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 2 years, 2 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sandman420]
#27675322 - 02/27/22 10:49 AM (4 years, 5 months ago)

Damn man, that's fucked. That's the one thing i didnt want to hear. I suppose that shit like that is almost impossible to get rid off and i should just toss my shit and start new ?

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sandman420
Saint PP

Registered: 06/17/04
Posts: 5,393

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]
#27675331 - 02/27/22 10:56 AM (4 years, 5 months ago)

Yes I wouldn't carry on with those 3 cultures you showed. Maybe try some new source. If a supplier had the problem then it could be in all of their stuff.

Even some bacteria are mycoparasites.

--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (02/27/22 10:57 AM)

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Dendrocopos

Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 2 years, 2 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sandman420]

1
#27675360 - 02/27/22 11:16 AM (4 years, 5 months ago)

would you deem this one as clean ? From the same mother plate as A.

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aosod]

1
#27675372 - 02/27/22 11:24 AM (4 years, 5 months ago)

aosod said:

I have some plates that i poured but did not put any spores into and they have the same problem, is it something with my cleaning or PC process? Or is it possible that condensation from them being on PC leaves some residue on the plates?

Now that's a start, I call it auto contam. So something is wrong
With your sterile tek. I say start your own thread and answer these.

:towelie:

Well that's a start, from there you continue your
conversation without getting lost.

--------------------
:sporedrop:

:sporedrop:

:sporedrop:

:sporedrop:

GETTING STARTED :sporedrop:

:sporedrop:

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sandman420
Saint PP

Registered: 06/17/04
Posts: 5,393

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]
#27675374 - 02/27/22 11:27 AM (4 years, 5 months ago)

Karlinator3000 said:

would you deem this one as clean ? From the same mother plate as A.

It looks good except the green mycelium at around 1 o'clock, but green agar so i don't know if it's cause of that.

--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras:

Dendrocopos

Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 2 years, 2 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sandman420]

1
#27675400 - 02/27/22 11:45 AM (4 years, 5 months ago)

Hehe, that's a shadow from the green marker thats used to mark.

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labbar
Stranger

Registered: 10/30/19
Posts: 331
Last seen: 3 months, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dendrocopos]
#27675478 - 02/27/22 12:41 PM (4 years, 5 months ago)

Jack Frost

APE

3xJf 2xNat

--------------------

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Libhunter2021
Stranger

Registered: 09/27/21
Posts: 19
Last seen: 4 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: labbar]
#27675590 - 02/27/22 01:56 PM (4 years, 5 months ago)

First transfer from a clone plate looking well I think, I could of dropped the transfer closer to the centre I guess. Clones had whispy irregular growth but after 1st transfer they are all looking a lot stronger and showing much better more regular growth.

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Keefersutherland
Strange

Registered: 09/02/20
Posts: 154
Loc: Upstate NY
Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Libhunter2021]

1
#27676340 - 02/27/22 10:50 PM (4 years, 5 months ago)

Made some transfers of some sketch GT and KSSS plates as well as throwing a couple chunks of said sketchy plates toe some test jars via hazyhorse's recommendation as I'm in kind of a hurry to get in a bunch of jars. We'll see what happens. The new plates seam toe be recovering nicely.

Here's a pan plate recovering. Going from agar>LI>grain(any recommendations on what type)>Baba Yaga's mono tek

Lastly big thanks to Mycoplex for the swabs. I can tell these are going to rip. will start cleaning up in a few days.

Another big thanks to Smartattack for the swabs. First time starting this variety.

Even though its early I think things are progressing well. I'll have a busy couple of weeks ahead of me. If anyone see's anything sketch let me know so I can address it.

Keef

--------------------

LAGM 2023

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Crackatoa
Stranger in a strange land

Registered: 03/31/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 11 months, 12 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Keefersutherland]

3
#27676388 - 02/27/22 11:22 PM (4 years, 5 months ago)

PE6 plate pin

--------------------

Crack's Tek's and Shit

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Shop:

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