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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] * 1
    #27654597 -

multifractal said:
Swabbed an albino riptide fruit and then rolled those swabs in a PEU×Melmak print. 4 plates that were streaked and/or have a swab jabbed in them are showing clean germination and if they remain clean I will use the plates to inoculate jars directly:


I also made several, like the one below, plates by placing transfer wedges taken from two different phenotype germination plates directly on top of each other. Each plate has two small stacks of wedges. I will probably let most of these plates pin out.


And just simply seeing if these two will intermingle meaningfully:




Oooooo me likey! I'd be cutting xfers like a MF! That swab stick is super wet - what's your agar recipe? Also I like your simple dot method for keeping track of what's what on the same plate, it's like a Lewis dot structure lol :thumbup:


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27654603 -

LME+Yeast but I use less than half the amount of agar I usually do and a little added corn syrup for plates intended to be used to germinate spores. The plates end up a little loose and wet, probably why the swab is saturated, but anecdotally I do notice quicker germination times overall and possibly stronger mycelium (for a germ plate). Sometimes I throw in a little cornmeal too.

Edited by multifractal (02/11/22 12:31 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Prakriti] * 3
    #27654740 -

Quote:
I thought maybe there was a funny story behind the name tiger drop, and maybe it could make for an interesting discussion. I had never seen the question asked. So yeah, when someone says “I found the answer in less than a minute using TFSE”, and then a second person responds with a link to search results for “tiger drop” (both very dickish responses), I got a little ticked and threw some shade back.


Surprise, I agree it was a bit "dickish". I found 80 results from TCs
for Tiger Drop. Read some of them and didn't really see the origin of it.

I confess the level of spoon feeding seems worse lately.
Sorry for all the fuss.

Aha! Beat ya! I win!  The Tiger Drop  :awedance:

Edited by Inthepit (02/11/22 06:49 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #27654785 -

All good my dude. Water under the fridge.
:chugbeer:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 2
    #27654841 -

nektar61 said:
ReverendMyc said:
So the attempt to take a transfer of myc from a fruiting jar back to agar didn't go that well.




Question for anyone: What's your general work rule on opening a plate with mold?

I've seen someone with years on here say they never open a plate with sporulating mold (turned from white to green/whatever). They said they'll only open a plate with bacteria and maybe mold before sporulation, because sporulating mold can get everywhere and infect your room / house.

I don't know this from experience, I heard it and I'm curious what others have to say.


I hear people say that a lot too and I think it's not true. There's trillions of mold spores in your house always, I don't think opening a moldy plate is going to significantly make your spore load worse.

When I am trying to salvage a moldy culture, I never work on other plates at the same time, because I don't want the mold spores to get everywhere inside my SAB. So I usually do moldy plates at the end of a SAB session, sterility be damned, because it's already moldy anyways. I open up the plate, take a transfer from the cleanest spot, and put it on a new plate. That new plate is 100% going to get mold on it, so I wait two or three days and take another transfer as soon as I see mold colonies popping up. But you've got to take that second transfer before they start sporulating.


--------------------


Important Links.

Be kind to others.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: joze] * 1
    #27654858 -

:whathesaid:
That is what I was going to say.
I don't usually bother unless it is a culture that I am really wanting to salvage. Also, I hear use a SAB rather than a flow hood/ffu. Or some have mentioned doing it at the end of a session in front of a hood just after turning it off, but I still would worry that this could increase the spore load on the face of the filter.

P.S. to say that I only kept those plates to see if any myc would eventually outrun the mold for a sample. If I was going to open it, I would have wanted to do it much earlier before the spores got that out of hand.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

Edited by ReverendMyc (02/11/22 08:47 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Prakriti] * 4
    #27654935 -

Prakriti said:
My bad for posting an agar-related question on an all things agar thread.


Just dont let it happen again


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27654988 -

bigfootscreepyuncl said:
colonized grain in a dime bag. I've even heard of putting a wedge into a dime bag. I suppose you could probably even swab some mycelium if you knew it was going to be shipped quickly and used immediately. Little <5ml vial of sterile water that you drop a transfer puck into and seal up


I saw somewhere 2ml plastic autoclave hardy vials being used for culture storage. Clean mycelium on agar in sterile water. If I wanted to send my wild oyster clone to a member, I feel like this would be a great way to do so…

If somebody accidentally sent a mislabeled speck of mycelium from an active species, I wonder if it would even contain a measurable amount of any scheduled alkaloids? Would genetic testing be required to determine the origin species?


--------------------
Two eyes to look, One eye to see.
Prying open my third eye

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MysticMycologist] * 1
    #27655137 -

What's the best method for isolating away from mold on agar? I have some mold that the cube myc is growing right along with, seemingly intertwined.

I did the sandwich tek with LME agar, and the white mold showed itself on top of the sandwich. Clumpy, disorganized looking white mold with a huge halo. The myc seems to have remained trapped under the sandwich.

Until then, I hadn't seen signs of it besides a big halo of clear growth surrounding the cultures, and when I put it to grain you can tell it smells contaminated, closer to mold than bacteria smell.

The original plate came from a BRF jar clone. What should I try at this point to isolate away from the mold? Rapid transfers, Warm pour? Saw RR recommend Antibiotic (gentamicin) sandwich method can actually help with molds? I didn't think gentamicin had an effect on mold, and that thread was pretty old.

Suggestions?

I know it doesnt look obvious, but this plate had mold.



All the transfers I made from it, and from the next plate I'll show, looked like this:




I then saw that this plate, had a "bubble" of agar near the outer edge of the plate, from when I'd poured the plate. I saw that some cleaner looking growth had grown through this bubble, but the bubble itself was still present. I took my scalpel, "popped" this bubble and made a couple transfers from it.

Here's the plate the xfers came from (inside the bubble):




These transfers are the best looking myc I've seen from this culture, and the best stuff I'm working with atm. To ENSURE that these are finally clean, what's my next best move?

This first one, was showing way healthier growth on one side, and the other side looks contamed. The side I took transfers from showed way healthier looking growth and had grown out alot farther than this weak looking side. I suspect I carried a bit of the mold along with it (the bubble was small and I may have grabbed some of the transfer from the edge of the bubble, where the mold still was). I took transfers already from the healthier looking side. Whats left is the sketchy side: (little dots arent bacteria, just lighting)





Here is the other new xfer, which I haven't xfered from yet. Should I grab that long piece of myc? Im worried that the mold is still present in this one too, and that that long piece of myc might be the piece that is outrunning the mold?  :




Previously, the only sign of the mold on agar was slow growth, a big clear halo outside of the myc, and weak transfers. When I sandwiched a piece of myc, the white mold showed itself (threw away or I'd have pics). The transfers from inside the bubble are the only ones showing this stronger growth.

Am I in the clear with this plate finally, or should I be worried? Should I rapid transfer this plate as well, or wait for it to grow out a bit more so I can observe it?

Edited by manlord (02/11/22 02:38 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: manlord]
    #27655440 -

nektar61 said:
ReverendMyc said:
So the attempt to take a transfer of myc from a fruiting jar back to agar didn't go that well.




Question for anyone: What's your general work rule on opening a plate with mold?

I've seen someone with years on here say they never open a plate with sporulating mold (turned from white to green/whatever). They said they'll only open a plate with bacteria and maybe mold before sporulation, because sporulating mold can get everywhere and infect your room / house.

I don't know this from experience, I heard it and I'm curious what others have to say.


You said you use a hood? Wait till the end of your work shut the flow off and get to any good myc quickly and efficently.Wait like 5-10 seconds after the flow stops and get moving. I've had really good luck this way. Fuck going back to a SAB.

Not seeing any semp there (maybe center of one plate?) if that's what ya said it was. I wish I didn't give out my whole print as I can't get these fuckers to pop either.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27655510 -

Hey yall just wanted to thank everyone here that has helped me along the way. Some of you have the patience of mother teresa lol

Anyway got some plates here:

This one looks good to me



the hairs are standing straight up



this one looking decent also



pretty fuzzy tho




both t3, from some tomentose looking plates
Both ready to go to grains? or T more…?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #27655516 -

The Fresh Prints said:
You guys are really beating a dead horse with this search engine/etiquette  lecture.





Nice plate!  If I may ask, what is your agar recipe for that (including food coloring ratio)?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27655533 -

rockyfungus said:
nektar61 said:
ReverendMyc said:
So the attempt to take a transfer of myc from a fruiting jar back to agar didn't go that well.




Question for anyone: What's your general work rule on opening a plate with mold?

I've seen someone with years on here say they never open a plate with sporulating mold (turned from white to green/whatever). They said they'll only open a plate with bacteria and maybe mold before sporulation, because sporulating mold can get everywhere and infect your room / house.

I don't know this from experience, I heard it and I'm curious what others have to say.


You said you use a hood? Wait till the end of your work shut the flow off and get to any good myc quickly and efficently.Wait like 5-10 seconds after the flow stops and get moving. I've had really good luck this way. Fuck going back to a SAB.

Not seeing any semp there (maybe center of one plate?) if that's what ya said it was. I wish I didn't give out my whole print as I can't get these fuckers to pop either.


i dont recommend shutting off your hood for that.  just align your plate so the myc your transferring is closer to the filter than the mold. also known as first air vs secondary air


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: teaker]
    #27655599 -

teaker said:
The Fresh Prints said:
You guys are really beating a dead horse with this search engine/etiquette  lecture.





Nice plate!  If I may ask, what is your agar recipe for that (including food coloring ratio)?


Thanks!

Roughly 16g agar, 16-18g LME (I'm all over the place in this range), 1L tap water. Food coloring is just added to taste-- one solid skeet is usually good :lol:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: san pedro guy] * 2
    #27655609 -

san pedro guy said:
Hey yall just wanted to thank everyone here that has helped me along the way. Some of you have the patience of mother teresa lol

Anyway got some plates here:

This one looks good to me



the hairs are standing straight up



this one looking decent also



pretty fuzzy tho




both t3, from some tomentose looking plates
Both ready to go to grains? or T more…?


I would send a couple wedges on the leading edge to grain AND transfer to new plates in a few days if it were me.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet] * 1
    #27655986 -

I’m going to call this

“Mission Impossible”

I have this clone culture I want to preserve and when I looked a week later I saw that I had apparently inoculated the dish liberally with penicillin or something like that when I did the transfer. I thought what the hell - challenge accepted! I identified a couple of spots I wanted to try to take a transfer from…



Then appreciating my flowhood would be worse than open air sterile work, I quickly fashioned a mini still air box with a Amazon box and some clear packing tape:



Then I held my breath and went to work:



I made two transfers onto mini plates. We will know in a few days if I brought a bunch of green stuff over with the mycelium!


--------------------
What are they saying? Listen carefully, it might be something you need to hear...




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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27655987 -

multifractal said:
LME+Yeast but I use less than half the amount of agar I usually do and a little added corn syrup for plates intended to be used to germinate spores. The plates end up a little loose and wet, probably why the swab is saturated, but anecdotally I do notice quicker germination times overall and possibly stronger mycelium (for a germ plate). Sometimes I throw in a little cornmeal too.


Did you say … “cornmeal”??

:awedance:

https://www.shroomery.org/forums/showflat.php/Number/20517365/page/5


--------------------
What are they saying? Listen carefully, it might be something you need to hear...




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Re: AGAR ENVY! (Anything and All things agar!) [Re: PsiloPsychIn]
    #27655994 -



Tell what you see. It was a print scraped off tinfoil and sprinkled. It started off good but has stalled. Somewhat whispy. Should I transfer part of the print. Another was suggested that I have wrong agar recipe. I'm running out of options with this strain.:gocanada:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CagedApe]
    #27656000 -

Looks like some lipstick mold, why does it have pink spots?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27656003 -

Pink agar

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