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Lectric
Stranger

Registered: 08/31/20
Posts: 102
Last seen: 5 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Wall.E]
#27373940 - 07/04/21 02:37 PM (5 years, 4 months ago)

Wall.E said:

Oh yeah, the second picture is bacteria lol. The first one looks better but I’d still transfer before sending

appreciate the feedback man!

I'll try to isolate that APE myc from the bacteria - grab a sample directly from the top growth.

Regardless, time for more germ plates :grin:

:grin:

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Nimpo
Big Black

Registered: 05/10/12
Posts: 2,699

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lectric]
#27374397 - 07/04/21 10:39 PM (5 years, 4 months ago)

Hey guys, how does this PE plate look to yall? It looks a little weak and sparse to me. How should I proceed?

PE T1 on agar, ingredients consist of:
1 cup water
4.5g agar
3 teaspoons potato flakes
1 drop honey

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Benson
The Kidd ⭐

Registered: 09/29/20
Posts: 898

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nimpo]
#27374572 - 07/05/21 02:28 AM (5 years, 4 months ago)

Nimpo said:

Hey guys, how does this PE plate look to yall? It looks a little weak and sparse to me. How should I proceed?

PE T1 on agar, ingredients consist of:
1 cup water
4.5g agar
3 teaspoons potato flakes
1 drop honey

This is exactly how my PE culture looks :shrug:

:shrug:

I'm guessing it just be that way sometimes. My first PE jar is like 90% colonized, fingers crossed :grin:

:grin:

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mushboy
modboy

Registered: 04/24/05
Posts: 41,005
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Benson]
#27374848 - 07/05/21 12:12 PM (5 years, 4 months ago)

i hate bubbles in my agar.

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BrownBear
Warrior-Traveler

Registered: 06/05/20
Posts: 1,539
Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27374851 - 07/05/21 12:14 PM (5 years, 4 months ago)

Bubles in my agar happens to me every once in a while. IMO, its not a big deal.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BrownBear]
#27374915 - 07/05/21 01:06 PM (5 years, 4 months ago)

I hate my new 1000ml pyrex slim line media bottles because they slosh and splash while I’m pouring making way more bubbles than I’m used to. You know me homie, I’m autistic and there comfort in symmetry and consistency. Things like bubbles or a myc disk off center can ruin my entire mood for the day.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27374959 - 07/05/21 01:37 PM (5 years, 4 months ago)

Speaking of symmetry

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Samushlove
Water

Registered: 03/09/21
Posts: 248
Loc: Foothills

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27374978 - 07/05/21 01:51 PM (5 years, 4 months ago)

D3monic said:

Speaking of symmetry

:allseeingeye:

:bow2:

:bow2:

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monkey_accident
seraphim

Registered: 05/11/19
Posts: 710
Loc: im here, bro
Last seen: 4 months, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Samushlove]
#27375040 - 07/05/21 02:47 PM (5 years, 4 months ago)

How does that top left plate look to y'all? more tomentose than the rest & the edge from ~10 to 12 o'clock looks a bit weak to me.
the rest imo look fine for grains whatcha think

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0ptiquest
Stranger

Registered: 07/05/20
Posts: 512
Last seen: 1 month, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: monkey_accident]
#27375079 - 07/05/21 03:16 PM (5 years, 4 months ago)

First plate posted here, hope the transfers go well.

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moshe1

Registered: 08/29/15
Posts: 378
Last seen: 4 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: 0ptiquest]
#27375408 - 07/05/21 07:50 PM (5 years, 4 months ago)

I realized its going to take a little longer to test each plate that i have ready- they've been sitting out for 1-2 weeks fully colonized. i just read that i shouldn't let my plates colonize to the edges to avoid contam risk but they've all pretty much done that already, so i went ahead and separated my top runners from every set i had and put them in the fridge just now. (wrapped in parafilm, then all together with saran wrap, then in ziploc bag). My initial idea was to let them grow out and sit for a little to see which dishes pin on agar so i could test those first.

how detrimental was my mistake of letting them get to the edge of my dishes? i finally have testers waiting for their turn for grains and i'd hate to have fucked it all up cause i didn't fridge them early.

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Edited by moshe1 (07/05/21 07:50 PM)

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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,346
Loc: Between me and you

Re: AGAR ENVY! (Anything and All things agar!)

[Re: moshe1]

1
#27375433 - 07/05/21 08:03 PM (5 years, 4 months ago)

Minimal risk if you're solid on sterile Tek and clean culture. Still not best practice, but if I'm confident on those other aspects, no worries.

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,225
Loc: Gaming the system
Last seen: 11 hours, 34 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: moshe1]

1
#27375435 - 07/05/21 08:06 PM (5 years, 4 months ago)

It’s fine. If you want to be extra careful just don’t use any myc from near the edge.

The edge is closest to the air so it’s easier for something to land there, but pretty much everything is obvious on agar. So if the edge looked clean a few days after you last opened the plate it should still be fine.

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27375467 - 07/05/21 08:30 PM (5 years, 4 months ago)

Has anyone ever had germ plates come out like the AA+ I have germinating here? It looks super concentrated and don’t know if this is looking right…

Also has anyone transfer what seemed to be straight rhizomorphic growth on to a plate and the plate comes on straight tomentos?

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,225
Loc: Gaming the system
Last seen: 11 hours, 34 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]

1
#27375471 - 07/05/21 08:33 PM (5 years, 4 months ago)

The aa+ is bacteria.

Myc can switch between growth types based on how the plates are made.

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monkey_accident
seraphim

Registered: 05/11/19
Posts: 710
Loc: im here, bro
Last seen: 4 months, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]
#27375474 - 07/05/21 08:34 PM (5 years, 4 months ago)

that AA on the left is some type of bacteria/mold no doubt. thats not myc
and yeah, myc can change from rhizo to tomentose and back and forth forever depending on conditions and agar medium recipe changes

edit: lol AKA beat me to it :whathesaid:

:whathesaid:

Edited by monkey_accident (07/05/21 08:34 PM)

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27375544 - 07/05/21 09:13 PM (5 years, 4 months ago)

Ok that makes sense. I lightened up ME by a gram per 500ml of H2O

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monkey_accident
seraphim

Registered: 05/11/19
Posts: 710
Loc: im here, bro
Last seen: 4 months, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]
#27375608 - 07/05/21 09:57 PM (5 years, 4 months ago)

If I remember correctly some guys were talking about adding nutritional yeast for more rhizomorphic growth
never tested it myself but worth a shot if you like the rhizo look

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Crackatoa
Stranger in a strange land

Registered: 04/01/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: monkey_accident]
#27375614 - 07/05/21 10:02 PM (5 years, 4 months ago)

Team yeast
:douchewink:

:douchewink:

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#27375621 - 07/05/21 10:08 PM (5 years, 4 months ago)

Crackatoa said:

Team yeast

Looks like I have some shopping to do lol.

Team yeast?? Whatever it takes to be apart of the Pheno Hunter gang 👉👈

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