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 BrownBear
Warrior-Traveler
Registered: 06/05/20
Posts: 1,539
Last seen: 12 days, 7 hours
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 BrownBear's Grow Log Adventure 
#27026321 - 11/07/20 08:35 AM (2 years, 6 months ago) |
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Welcome fellow Shroomerites to BrownBear's Grow Log Adventure. Feel free to give me tips and/or critique my process in anyway that is necessary for my success. I am here simply to learn. My goal is to become a highly efficient, dense af, canopy producer. With all of that said, lets move on to my process so far.
I brought 2 full quarts of oats to a boil. Turned off the heat and let steep for 30 minutes with lid on pot. I filled the water twice as high as the oats. After 30 minutes the oats had plumped up and met the water level. I strained over top of my bathtub with a window screen.
I left them overnight. I made sure the wife wasn't planning on using the shower for atleast 8 hours.  I want them evenly dry on the outside so I mixed the grain and spread it out a few times during this stage. I also picked out most of the busted grain. Lost grain was under 28 grams. Once I was satisfied with the moisture level of the grain I weighed the grain. 1080 grams of dry oats equals 1946 grams of hydrated oats at 44% hydration.
If my calculations are correct than my hydrated oats hold around 44% water by weight. I want to experiment with no prep oat master jars. If I use 78 grams of dry oats per pint and add 62 grams of water that will give me 140 grams of hydrated oats per pint.
I am only filling the jars half way because I want to slurry them after full colonization. My plan is to use one master to inoculate 7 quarts of oat supplemented bulk bags. If I were going to use them for g2g purposes I would use 200 grams of hydrated oats per pint master jar. I would use 112 grams of dry oats and 88 grams of water per pint jar.
A budy of mine was nice enough to give me some live cultures. I am so thankful. This is going to give me such a huge head start. I was given TWC, WG clone, ESS clone, APEU clone and a TYC clone. I made blended LI's with a half a plate from each of them. I used the other half of each plate to transfer to new plates.
TYC on the left and TWC on the right.
APEU LI syringe.
I wasn't satisfied with my agar recipe up to this point. It seemed too soft and not nutritious enough. The plates I received were pretty firm. So with these new plates I used 15 grams of agar powder instead of 10 that I was using per 500ml of water. I also used 10 grams of brf instead of the 5 grams that I was using. Hopefully this works out well. I do have some MEA and 4 more sleeves of plates on the way.
I made 14 oat master pint jars. Each jar contains 139 grams of hydrated oats. I am currently pc'ing the grain for 2.5 hours at 13.5 psi. I plan on inoculating 3 jars of each culture except I have to choose which culture only gets two jars. I would have made it even with 15 jars but I needed to sterilize my plastic ship lids for my LI's and sterilize a couple syringes. The specs on my 921 say it can hold 19 regular mouth pint mason jars. I'm not so sure about that. I may be able to fit 16 in it.
I guess the only question I have is how much LI would be appropriate for each oat jar?
I will continue to update this grow log as things progress. Hopefully by the end I will have dense af canopies like many of you great folks share with this community.
Edited by BrownBear (11/07/20 09:44 AM)
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 eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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 Re: BrownBear's Grow Log Adventure [Re: BrownBear] 1
#27026353 - 11/07/20 08:50 AM (2 years, 6 months ago) |
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Plates i use for LI are very firm so when its blended, its a jellylike substance that sticks to the grain when inoculated. This way when you shake, you can visibly see the pieces sticking to grain. I use pastys recipe but agar powder instead of the asian flaked agar. Its like double the amount of agar if its in poweder form. So i generally do 1 tablespoon of agar to pastys normal agar recipe instead of 2.
Damn i like that grain build, simple enough and can pack away easily! 
Edited by eatyualive (11/07/20 08:58 AM)
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BrownBear
Warrior-Traveler
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Re: BrownBear's Grow Log Adventure [Re: eatyualive]
#27026452 - 11/07/20 09:51 AM (2 years, 6 months ago) |
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I put that grain build together on a whim. Works great for small batches of grain. When I do bulk bags I am simply going to strain the grain and then imediately add it to my coir mixture. So I don't need anything bigger.
How much LI would you recommend per pint jar of oats?
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada 
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27026543 - 11/07/20 10:40 AM (2 years, 6 months ago) |
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following
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Edited by SunnyDayze (11/07/20 10:48 AM)
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BrownBear
Warrior-Traveler
Registered: 06/05/20
Posts: 1,539
Last seen: 12 days, 7 hours
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Re: BrownBear's Grow Log Adventure [Re: SunnyDayze]
#27026562 - 11/07/20 10:53 AM (2 years, 6 months ago) |
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Good morning!
I feel your struggles. I was having a hell of a time trying to get something going with old spore prints and syringes. Indeed things should go better for you next time. Practice makes perfect and as long as you learn from your mistakes you will improve. I think the quote goes something like this. "The wise never knows all, only fools know everything."
How much LI should I use to inoculate each pt oat jar?
Edited by BrownBear (11/07/20 11:07 AM)
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27026679 - 11/07/20 11:49 AM (2 years, 6 months ago) |
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haha sorry that other post was meant for the tribe is why i edited it.
I haven't ever done LI (only LC) but I would do a small squirt 2-3 ml for a pint would be my guess but Eat will let you know I'm sure.
I'm also thinking you can put 16 jars in if you put the last two on their side, middle top. I usually run my pc around 17-18, 2.5 hours for quarts.
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BrownBear
Warrior-Traveler
Registered: 06/05/20
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Last seen: 12 days, 7 hours
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Re: BrownBear's Grow Log Adventure [Re: SunnyDayze]
#27026710 - 11/07/20 12:07 PM (2 years, 6 months ago) |
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No worries. I noticed you had edited the post and copy and pasted it in the tribe thread after I responded. I just read eats LI tek and he uses 1cc per quart so I guess I should probably use less than that. I cannot get my pc to stay at 15 psi without letting the weight rock and hiss like crazy. Do you think I should incease my pc time or find a way to get the pressure up higher?
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27026738 - 11/07/20 12:24 PM (2 years, 6 months ago) |
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I've always used AA's with the petcock so never had that issue but heard that you can put a bit of extra weight on it to make it better.
I believe that you will be fine with your pressure and time though for the pints. Quarts push it up to 3 to 3.5 hours.
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BrownBear
Warrior-Traveler
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Posts: 1,539
Last seen: 12 days, 7 hours
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Re: BrownBear's Grow Log Adventure [Re: SunnyDayze]
#27026748 - 11/07/20 12:32 PM (2 years, 6 months ago) |
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Is a petcock an accessory that I can buy and install on my 921? I don't plan on going to quart jars but I do plan on using the grain slurry to inoculate oat supplemented bulk substrate bags. Would 3.5 hours be okay for those or should I increase to 4+ hours? If thats the case I may want to look into one of those petcocks.
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27026762 - 11/07/20 12:39 PM (2 years, 6 months ago) |
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oh right! you did say it was a 921. yes you can order one. I've always gotten my replacement parts at redhillgeneralstore.com. They are great to deal with.
Edit:
Forgot they no longer manufacture the petcock for the AA's. I know I found one somewhere else but having a hell of a time trying to find it again.
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Edited by SunnyDayze (11/07/20 12:48 PM)
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BrownBear
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Re: BrownBear's Grow Log Adventure [Re: SunnyDayze]
#27027104 - 11/07/20 03:57 PM (2 years, 6 months ago) |
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I inoculated all 14 grain master jars today. After a 2.5 hour pc run at 13.5 psi I let the pc cool enough to carry it down stairs into my basement and into the clean room without pot holders. I turned the flowhood on and let it run for about 15 minutes. Then I opened the pc and placed jars in front of the hood to finish cooling. They weren't hot but they were a little warm.
I then took the jars and placed them out of the way to make room to make a LI syringe for each of the 5 cultures I am working with. I first made the LI syringe then noc'd up the three grain jars before moving onto the next culture.
I wiped everything from top to bottom with 71% iso before putting it in front of the hood. I also flamed sterilized the entire 3" needle before every inoculation (finishing the flame sterilization at the tip until it was red hot). I also iso'd my gloves before every step in front of the hood.
I ended up using about 3 cc's per jar. I figured I would just split the syringe three ways. After a good shake none of the jars are showing any excess moisture on the bottom nor on the grain. There does appear to be moisture on the upper part of the mason jar. I'm sure that will dissipate over the next couple of days though.
  
I just want to let you all know before you take notice. You can critique anything and everything except my excessive use of parafilm.
Edited by BrownBear (11/07/20 04:04 PM)
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27027836 - 11/08/20 01:02 AM (2 years, 6 months ago) |
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Definitely no critiquing here but I am wondering about the parafilm. Do you have any GE holes in your lids? Why did you use parafilm around the jars?
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BrownBear
Warrior-Traveler
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Last seen: 12 days, 7 hours
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Re: BrownBear's Grow Log Adventure [Re: SunnyDayze]
#27027997 - 11/08/20 06:12 AM (2 years, 6 months ago) |
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I wrap the entire lid on my LC's and LI's after tightening down the lid. I cover the ship after every use. I don't trust ships to heal properly. I also do not like the way the syringe filter screws on with no gasket in between it and the lid. The parafilm prevents any potential leaking of liquids.
I also use the parafilm on the grain jars. Those are unmodified metal lids in which I flip the lid as to not allow it to seal to the jar during sterilization. After inoculation I do not completely tighten the lid. I close it until there is a slight resistance. Then I wrap the entire lid with parafilm. The parafilm allows safe gas exchange on the grain jars.
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27028500 - 11/08/20 12:09 PM (2 years, 6 months ago) |
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Nice, thanks for explaining  . Haven’t seen that before but there’s lots I haven’t seen lol
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BrownBear
Warrior-Traveler
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Posts: 1,539
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Re: BrownBear's Grow Log Adventure [Re: SunnyDayze]
#27028597 - 11/08/20 01:17 PM (2 years, 6 months ago) |
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Yea. No problem. I have a habit of picking up my jars where the lid meets the jar. Probably not a good idea with unmodified lids. The parafilm should close that contam vector though. I also used to have problems with plastic lc jar lids leaking. Not anymore.
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BrownBear
Warrior-Traveler
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Last seen: 12 days, 7 hours
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Re: BrownBear's Grow Log Adventure [Re: BrownBear] 1
#27030512 - 11/09/20 04:52 PM (2 years, 6 months ago) |
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Update:
I have some really nice TYC T4 clones on agar. These were transfered 4 days ago. I used 10 grams of brf and 15 grams of agar powder per 500 ml of filtered water. I think I may have finally got something right. What do you all think?
  
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Sold Out Online
Stranger Danger
Registered: 09/05/20
Posts: 264
Last seen: 11 months, 6 days
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27030536 - 11/09/20 05:24 PM (2 years, 6 months ago) |
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I think your pressure cooker needs to run at 15 PSI.
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BrownBear
Warrior-Traveler
Registered: 06/05/20
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Last seen: 12 days, 7 hours
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You're right. But I don't want to try anything dangerous. So I may have to just increase my sterilaztion time. Do you think I could be not adding enough water? That is the only thing that I can think of as to why the pressure won't get up that high.
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Munchauzen
Registered: 06/22/11
Posts: 14,335
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Re: BrownBear's Grow Log Adventure [Re: BrownBear]
#27030651 - 11/09/20 07:06 PM (2 years, 6 months ago) |
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Quote:
Iambrownbear said:
No worries. I noticed you had edited the post and copy and pasted it in the tribe thread after I responded. I just read eats LI tek and he uses 1cc per quart so I guess I should probably use less than that. I cannot get my pc to stay at 15 psi without letting the weight rock and hiss like crazy. Do you think I should incease my pc time or find a way to get the pressure up higher?
tape a couple quarters to the weight rocker. I have mine dialed in so that it starts just slightly hissing at 15 psi.
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BrownBear
Warrior-Traveler
Registered: 06/05/20
Posts: 1,539
Last seen: 12 days, 7 hours
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Re: BrownBear's Grow Log Adventure [Re: Munchauzen]
#27030677 - 11/09/20 07:20 PM (2 years, 6 months ago) |
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Thanks, munch. I'll give it a try.
It worked like a charm. Thanks again. 
 
Edited by BrownBear (11/10/20 07:08 PM)
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