AGAR ENVY! (Anything and All things agar!) - Mushroom Cultivation - Shroomery Message Board

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,217
Loc: Gaming the system
Last seen: 8 hours, 45 minutes

Re: AGAR ENVY! (Anything and All things agar!)

2
#27242104 - 03/07/21 04:05 PM (5 years, 6 months ago)

From the spore print. :eek:

:eek:

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Extras:

The Fresh Prints
Smell ya later

Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27242111 - 03/07/21 04:10 PM (5 years, 6 months ago)

Lol that was a plate I finger fucked and left open for 24 hours in my grow room 😬. Gnarly shit

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Nichrome
Skank

Registered: 12/17/18
Posts: 8,920

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Fresh Prints]
#27242188 - 03/07/21 05:40 PM (5 years, 6 months ago)

You have a healthy biome going on there in your grow room.

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Question everything, it's very responsive.
culture storage

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The Fresh Prints
Smell ya later

Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Fresh Prints]
#27242192 - 03/07/21 05:43 PM (5 years, 6 months ago)

Just for fun. Mystery plate pin cap "clone"

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Extras:

The Fresh Prints
Smell ya later

Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27242195 - 03/07/21 05:45 PM (5 years, 6 months ago)

Nichrome said:

You have a healthy biome going on there in your grow room.

For real. I was horrified when all that shit started popping up.

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,590
Loc: Hollywoo
Last seen: 1 hour, 19 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27242361 - 03/07/21 07:52 PM (5 years, 5 months ago)

Nichrome said:

You have a healthy biome going on there in your grow room.

Wonder if you could transfer that hellscape, add a good shroom clone slice, then use multiple transfers / then selection to grow mold-resistant bacteria-resistant shrooms?

I wonder if someone trying would end up with them in the hospital on a ventilator?

--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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mushboy
modboy

Registered: 04/24/05
Posts: 40,467
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

1
#27242407 - 03/07/21 08:33 PM (5 years, 5 months ago)

then this agar thread would have zero use :sad:

:sad:

drag

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:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Nichrome
Skank

Registered: 12/17/18
Posts: 8,920

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27243495 - 03/08/21 03:27 PM (5 years, 5 months ago)

Fucking around with anthocyanin in my mea. As the mycelium moves it eats the blue and turns it pink. Pretty sure this is due to enzyme production as the discoloration happens out in front of the leading edge (where no hyphae are present) to varrying degrees depending on the culture. Spores germinating seem to eat up the blue a lot more.

I have yet to notice any bacteria in my blue plates. Soon I'll do a test and move some from another plate onto a blue one to see if it dies or spreads.

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Question everything, it's very responsive.
culture storage

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Weedy

Registered: 01/11/09
Posts: 108
Last seen: 3 years, 4 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27243830 - 03/08/21 06:41 PM (5 years, 5 months ago)

APE clone

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Crackatoa
Stranger in a strange land

Registered: 03/31/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 1 year, 1 day

Re: AGAR ENVY! (Anything and All things agar!)

1
#27243843 - 03/08/21 06:47 PM (5 years, 5 months ago)

Pink buffalo and WG, a little yeast in this agar recipe.

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Crack's Tek's and Shit

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mushboy
modboy

Registered: 04/24/05
Posts: 40,467
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]

2
#27243911 - 03/08/21 07:26 PM (5 years, 5 months ago)

micro penis for dinner :dealwithit2:

:dealwithit2:

--------------------
:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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NickShade
Macronutrient

Registered: 10/09/14
Posts: 272
Loc: Earth
Last seen: 2 years, 10 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27244024 - 03/08/21 08:40 PM (5 years, 5 months ago)

Doinks

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bodhisatta said:
figure it out listen to the cake

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,590
Loc: Hollywoo
Last seen: 1 hour, 19 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27244363 - 03/09/21 04:34 AM (5 years, 5 months ago)

mushboy said:

micro penis for dinner

Mushboy:
Are plins (plate pins) always good candidates for cloning? Are they always likely among the better genetics for a particular MS plate?

thank you

--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras:

ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 3,485
Loc: uacious, but can't spel gud

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nektar61]
#27244644 - 03/09/21 08:41 AM (5 years, 5 months ago)

NEWS FLASH!

Caught on film. Lion escaping pen.

germ plate getting out of hand. this is the nursery plant "parafilm" type tape. I guess it does allow some fae. Just not enough to satisfy the Lion's Mane.

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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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King87
Stranger

Registered: 06/04/16
Posts: 60
Last seen: 3 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ReverendMyc]
#27244758 - 03/09/21 10:12 AM (5 years, 5 months ago)

Thought I'd share my latest noob fuck up with agar for your entertainment.

After months of trying to get a couple of solid looking plates, I finally took the 'plunge' to go to agar 2 grain in a SAB using the tic tac toe method.

Im using PP5 plastic containers. I put the lines pattern on the agar and sort of put a line all the way around the outer edge, then turned the pot upside down directly over the quart receiving jar and tried to give it a squeeze/shake/tap but I ended up having to use my scalpel again to scrape it into the jar because it was stuck to the pot.

Then... when I shook the jars, the little pieces instantly stuck to the upper sides of the glass and for the life of me I cant get them off the jar walls and into the grain, Ive shaken and bashed a lot! And the idea of going back in the SAB to scrape the walls sounds like a terrible idea.

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Crackatoa
Stranger in a strange land

Registered: 03/31/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 1 year, 1 day

Re: AGAR ENVY! (Anything and All things agar!)

[Re: King87]

1
#27244772 - 03/09/21 10:21 AM (5 years, 5 months ago)

Yeah, I've had to lay my jars in weird ways to get the grain on the agar that's stuck to the sides. Usually after a few days I'll go back shake and it'll come right off. Only had 1 stay stuck and it was half a 90mm petri. Before I drop a wedge I'll shake ask my grain to one side and let the wedge fall on the empty side and then just gotta shake since grain on top and done.
I've noticed several people don't do this, after you shake your jars. Pack it like a pack of cigarettes. You want all of your grain as close to each other as possible. It's easier for myc to spread if all the grains are touching. I feel like I see jars with allot of gaps.

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Crack's Tek's and Shit

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joze

Registered: 11/10/20
Posts: 1,058
Loc: PNW

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#27244783 - 03/09/21 10:26 AM (5 years, 5 months ago)

I feel like such a moron right now. I transferred four plates a few days ago, and one of the first plates I transferred was a PESA isolate that had a patch of mold. Today, three of the four transfers have identical small mold spots on them. I'm guessing it's because I opened up that moldy PESA plate and some spores started floating around inside my SAB, which then affected the next transfers I made.

I guess the best solution is to handle any plates with mold in a separate SAB session, or do them last?

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Important Links.

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Hide
I Have No Bung Hole

Registered: 11/25/14
Posts: 159
Last seen: 1 year, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: joze]
#27244784 - 03/09/21 10:27 AM (5 years, 5 months ago)

Yeah doing them last will help.

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clockworkshroom
Stranger

Registered: 02/20/20
Posts: 689
Last seen: 7 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Hide]
#27245024 - 03/09/21 01:23 PM (5 years, 5 months ago)

I had the same Joze, what does your mold look like?

I've finally got rid of it after a couple of transfers. I've done them in same SAB sessions but each time gone from cleanest to dirtiest.

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p9hu7's clean spawn thread

Extras:

joze

Registered: 11/10/20
Posts: 1,058
Loc: PNW

Re: AGAR ENVY! (Anything and All things agar!)

[Re: clockworkshroom]
#27245251 - 03/09/21 03:24 PM (5 years, 5 months ago)

clockworkshroom said:

I had the same Joze, what does your mold look like?

I've finally got rid of it after a couple of transfers. I've done them in same SAB sessions but each time gone from cleanest to dirtiest.

I think it's probably penicillium. White colony with a green/blue center.

--------------------

Important Links.

Be kind to others.

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