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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 14 days, 8 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Normlizer420]
#26925072 - 09/08/20 06:01 PM (5 years, 11 months ago)

Yes. Good to see members from almost 20 years ago still around!

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 2 days, 5 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26925175 - 09/08/20 06:57 PM (5 years, 11 months ago)

I’ve had a couple swabs that I streaked a bunch of plates and got nothing until I cut the whole handle off and jammed the swab into the agar.

Some people do the smarter version and tweezer some threads off to stick in the plate but I couldn’t get any to come loose.

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Darkshadow
Waiting In The Shadows

Registered: 06/11/20
Posts: 55

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26925448 - 09/08/20 10:42 PM (5 years, 11 months ago)

Thanks for the reply, I think I'll just have to transfer out what I come across and see what happens, learning experience.

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Ignored by me? Leave a rating.

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PNDub
Traveler

Registered: 05/19/10
Posts: 52
Last seen: 5 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Darkshadow]
#26926520 - 09/09/20 03:11 PM (5 years, 11 months ago)

.

Edited by PNDub (09/24/20 03:21 AM)

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redpillcottage
Mr Fantastic

Registered: 07/28/20
Posts: 45
Loc: United Kingdom

Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PNDub]
#26927875 - 09/10/20 09:15 AM (5 years, 11 months ago)

PNDub said:

This is my first attempt with agar, looking for some opinions, please! Do you see anything worth transferring?? Any and all feedback welcome.

These are from a print I took from a huge Amazonian cap I harvested:

Anyone see anything they would transfer? Are the pics clear enough? I've done TONS of reading on this, would love some feedback if anyone has a spare minute!! Thank you

Hey man, I don't know which ones are best to harvest, but if you find out, can you tell me? I have some at the same stage by the look of it 10 days.

My plan was to just start picking out bits that start looking ropey and grain spawn from that but not sure now. what do you know?

Cheers

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====================================

We're all going to die

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Funky Monkey
Human Suppository

Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot

Re: AGAR ENVY! (Anything and All things agar!)

[Re: redpillcottage]
#26927898 - 09/10/20 09:28 AM (5 years, 11 months ago)

Hey red,

:grin:

it is perfectly acceptable for you to post pics as well to ask people about yours too.., no need to wait for people to give advice to some random dude for his trays, as his trays are not your trays lol :lol:

:lol:

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26928040 - 09/10/20 10:52 AM (5 years, 11 months ago)

So this is an all out attempt to reduce the rate of auto-contams running about 30-40% currently.
Just say no to immaculate contamination!

9/9 Wednesday - Plates, Corning Borosilicate 100x15mm
20 plates in foil oven sterilized, 400 F, 2 hrs. ... should be 450 F
Cool down in oven 2 hrs
Carry in shoeboxes to SAB

9/10 9:00 Thursday - “Team Telephone” Agar
8g 1.6% Agar, 7.5g 1.5% Pilsner LME, green, no broth
Soak LME in 500mL bottled water before - 1 hr
Agar 20 min PC, took at least 20 to cool till pressure lock clicked.
Then opened the PC and tightened the media cap with foil still on.
Then off to the SAB to cool to 120F, this time.
Washed 90 Qrt SAB, rack, and table, w/Clorox & towel
Books on top of SAB to anchor it down and reduce effects of bumping.
Wet towel under 3” high rack. Foil wrapped plates in SAB.
Pour in 2 hrs.
What a pain taking the foil off in the SAB
Media bottle ring fell off, but only a couple drips.
I marked the drip spots on each plate with a Sharpie.
Maybe 200mL left over, some extra plates on top, to reduce condensation, of 4 stacks of 5 ea. 11:00
Oooh, really clear green!
Not going to let them sit too long before wrapping stacks of 5.
Trying to avoid auto-contam, immaculate contam.
But if there’s too much condensation,
then I’ll try putting a plate sleeve on them and cool next time...
Also this time, I have a sheet over the SAB,
because I might have a visitor.
12:00 wrapped! That’s a wrap! :awedance:

:awedance:

--------------------
:sporedrop:

:sporedrop:

:sporedrop:

:sporedrop:

GETTING STARTED :sporedrop:

:sporedrop:

Extras:

PNDub
Traveler

Registered: 05/19/10
Posts: 52
Last seen: 5 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: redpillcottage]
#26928154 - 09/10/20 12:03 PM (5 years, 11 months ago)

.

Edited by PNDub (09/24/20 03:21 AM)

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Keefersutherland
Strange

Registered: 09/02/20
Posts: 154
Loc: Upstate NY
Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PNDub]
#26928541 - 09/10/20 03:41 PM (5 years, 11 months ago)

Hey. So I’m on my second transfer of PE. I cut a large wedge of some rhyzomorphic myc which has continued to grow but it’s hovering over the new agar. My question is would you guys cut the wedge into thirds while the myc is strong and place onto new plates or just let it keep growing out until it hits the plate on its own. I know it was a large transfer but I’m still not great with the scalpel and I’m paranoid about contams so I was working quickly. I’m working on purging my agar thinner so this doesn’t happen again.

Another question. Do you guys transfer as soon as you think you have contamination on the plate or do you wait until the myc grows out but is still away from contamination. The plate has a spot of bacteria but it’s small so far. Thanks!

--------------------

LAGM 2023

Extras:

tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 14 days, 8 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Keefersutherland]

1
#26928545 - 09/10/20 03:45 PM (5 years, 11 months ago)

I would transfer in 2 or 3 days personally.

Mold i try to transfer away from asap. But bacterial sattelites usually stay put

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

sh4d0ws
LSx

Registered: 02/26/08
Posts: 12,089

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Keefersutherland]
#26928550 - 09/10/20 03:48 PM (5 years, 11 months ago)

Depends what the contam is.

Mycelium generally can "outrun" bacteria efficienctly enough that waiting for it to grow out more will work well.

If it's thought to be contaminated with some type of sporulating mold...well then transfering ASAP is ideal.

So there are different answers I guess depending on what you're dealing with. Hopefully that provides some insight. Surely some other members have advice on this too.

I'm working on a RW clone culture but the mycelium is looking iffy. I think it might be the formula I've been using for agar as of late but IDK. Might try to whip up a batch with some grain soak water, see if I can get better looking growth :confused:

:confused:

It's a seriously kick ass clone so, I reallly don't want to give up. Worried it has some hidden mold or something but who knows. The investigation continues

edit:

I really need to get on my slant game. Kind of embarassing to admit I've never made a slant in my life

--------------------

Extras:

sh4d0ws
LSx

Registered: 02/26/08
Posts: 12,089

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26928551 - 09/10/20 03:49 PM (5 years, 11 months ago)

tryptkaloids said:

I would transfer in 2 or 3 days personally.

Mold i try to transfer away from asap. But bacterial sattelites usually stay put

Basically what I said in 1 sentence instead of several :lol:

:lol:

trypt

:thumbup:

--------------------

Extras:

tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 14 days, 8 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sh4d0ws]
#26928561 - 09/10/20 03:52 PM (5 years, 11 months ago)

I need to make slants too.

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

mcnugget
Stranger

Registered: 06/22/13
Posts: 283
Last seen: 2 months, 8 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26928605 - 09/10/20 04:24 PM (5 years, 11 months ago)

How does this plate look? I've never had one try to pin so hard in the dish. Worried something might be off.

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Keefersutherland
Strange

Registered: 09/02/20
Posts: 154
Loc: Upstate NY
Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mcnugget]
#26928646 - 09/10/20 04:48 PM (5 years, 11 months ago)

Thanks. I’ll wait a few days and post the results.

--------------------

LAGM 2023

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Shu

Registered: 11/20/11
Posts: 888
Loc: PA, USA and FL, USA
Last seen: 2 days, 16 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26928653 - 09/10/20 04:54 PM (5 years, 11 months ago)

ruK said:

Lil B+ snowflake on PDA.

That's some clear pda you made. How did you filter it?

Extras:

Alvina
Stranger

Registered: 05/12/20
Posts: 6
Last seen: 4 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#26928981 - 09/10/20 08:33 PM (5 years, 11 months ago)

hey y'all
first time posting
first time pouring agar (telephone+LME)

first run was a little bumpy... er... chunky... but poured some decent plates and learned a lot along the way, pumped to get something growing on these ^_^

s/o to everyone on here for the knowledge this place is incredible <3

Edited by Alvina (09/10/20 08:45 PM)

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roarkell
The Roar Master!

Registered: 08/29/19
Posts: 2,223
Loc: Southern WI

Last seen: 2 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Alvina]
#26928998 - 09/10/20 08:45 PM (5 years, 11 months ago)

Alvina said:

hey y'all
first time posting
first time pouring agar (telephone+LME)

first run was a little bumpy... er... chunky... but poured some decent plates and learned a lot along the way, pumped to get something growing on these ^_^

s/o to everyone on here for the knowledge this place is incredible <3

What are you gonna put on those? I'm at the tail end of a spore print giveaway if you need something to put on those plates! Shoot me a pm if you're interested!

Extras:

seand04
It is what it is

Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 20 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: roarkell]
#26929030 - 09/10/20 09:04 PM (5 years, 11 months ago)

First PF classic transfer to MEA

Extras:

Alvina
Stranger

Registered: 05/12/20
Posts: 6
Last seen: 4 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: roarkell]
#26929037 - 09/10/20 09:07 PM (5 years, 11 months ago)

roarkell said:

Alvina said:

hey y'all
first time posting
first time pouring agar (telephone+LME)

first run was a little bumpy... er... chunky... but poured some decent plates and learned a lot along the way, pumped to get something growing on these ^_^

s/o to everyone on here for the knowledge this place is incredible <3

What are you gonna put on those? I'm at the tail end of a spore print giveaway if you need something to put on those plates! Shoot me a pm if you're interested!

I've got some golden teacher LC en route and a probably filthy cambodian print from my first grow earlier this year :crazy:

:crazy:

I'll slide you a PM :grin:

:grin:

Edited by Alvina (09/10/20 09:08 PM)

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